Fenbendazole에 저항성과 감수성을 지닌 염전위충의 분비배설물에서의 DNase 활성 비료

곽동미,Kwak, Dongmi 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3

Change in ${\beta}$-tubulin nucleic acid and protein sequences was the only known difference between Haemonchus contortus fenbendazole (FBZ)-resistant and -susceptible isolates. This change was sufficient to determine the pathologic effect induced by FBZ treatment. This research was initiated to investigate further differences from these two isolates. Since ${\beta}$-tubulin is involved in formation of microtubule, which has functions in secretory vesicle transport, DNase activities from excretory/secretory products (ESP) of the two isolates were compared, based on pH, sensitivity to DNase inhibitors, molecular masses and production of 3'-OH. The most significant difference detected was that a 38.5 kDa DNase activity was identified from ESP of H. contortus FBZ-susceptible isolates but not from those of H. contortus FBZ-resistant isolates. However, it was shown that the 38.5 kDa DNase is expressed with similar level of activity in intestine and whole worm of H. contortus FBZ-resistant and -susceptible isolates. This result demonstrated that the secretory transport pathway of the 38.5 kDa DNase was inhibited by unknown mechanisms, which may be related with ${\beta}$-tubulin sequence change in FBZ-resistant isolates. Other DNases of 34, 36 and 37 kDa were detected from ESP of both H. contortus FBZ-resistant and -susceptible isolates. Overall DNase activities found from ESP of these two isolates were not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. In addition, DNase activities in two isolates produced DNA fragments with mixtures of 3'- hydroxyls (OH) and 3'-phosphates (P) at each pH although the 3'-end labeling ratios at pH 5.0 and 7.0 were shown different. Identification of inhibition of the 38.5 kDa DNase secretion in FBZ-resistant isolates suggests existence of further differences, in addition to ${\beta}$-tubulin sequence change, in two isolates. This shows complex effect of FBZ on H. contortus biological mechanisms.

염전위충(Haemonchus contortus) 생식기관의 DNase활성에 관한 연구

곽동미,Kwak, Dongmi 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3

DNase activity in Haemonchus contortus reproductive tissue was characterized and compared to that in whole worm. DNase activity in reproductive tissue was detected throughout pHs 4-10 with high activity under acidic conditions. The activity was not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. The activity produced DNA fragments with mixtures of 3'-hydroxyls (OH) and 3'- phosphates (P) at each pH. Three distinct DNase activities were identified and had $M_rs$ of 34, 36 and 38.5 kDa in zymograms, which were distinguished according to pH requirement and sensitivity to EDTA. Among them, the 36 kDa reproductive tissue DNase had predominant activity at pH 5.0, but very weak at pH 7.0, and this activity was not inhibited by EDTA at pH 5.0. These characteristics of the 36 kDa reproductive tissue DNase resemble those of classic acidic DNases. In contrast, 36 kDa whole worm DNase activity had high activity at both pH 5.0 and 7.0. While the 36 kDa DNase activity at pH 5.0 was similar in both reproductive tissue and whole worm samples, the activity at pH 7.0 was predominantly detected in whole worm sample. This suggests that the 36 kDa whole worm DNase at pH 5.0 differs from that at pH 7.0. Thus, results indicate that the EDTA-insensitive 36 kDa DNase at pH 5.0 is specific for H. contortus reproductive tissue.

동물원 새끼 사자의 Trichophyton mentagrophytes 감염과 사육사에 전파된 원형피부백선 증례

김규태,이승헌,곽동미,Kim, Kyoo-Tae,Lee, Seung-Hun,Kwak, Dongmi 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.2

Dermatophytosis was found on the right front leg of a 4-month-old female African lion cub (Panthera leo) kept at a zoo with locally marginal alopecia. For diagnosis, culturing on sabouraud dextrose agar was performed and skin scrapings from the lesion were analyzed. The ones from the culture and skin scrapings were identified as Trichophyton mentagrophytes. A zookeeper that had been in contacted with the lion for artificial rearing developed skin lesions with well-defined erythematous plaques on the right arm about 1 month after the lesion in the lion was observed. The ringworm was probably transmitted from the lion through continuous contact.

아메리카 흑곰에서 발생한 소장 염전에 의한 장 파열 증례

김규태,이승헌,곽동미,Kim, Kyoo-Tae,Lee, Seung-Hun,Kwak, Dongmi 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.3

Intestinal rupture caused by small bowel volvulus was diagnosed in a captive 13-year-old male American black bear. The animal presented with decreased appetite, depression, lethargy, and mild abdominal distention for 3 days. The animal was treated with antibiotics, hypermetabolites, and digestive medicine daily; however, it died on the third day of treatment. The clinical symptoms included hemorrhagic ascites, gaseous extension of the small intestine, and intestinal rupture caused by small bowel volvulus. Hemorrhagic signs were observed in the lungs and heart. This is the first case to describe small bowel volvulus in mammals of the family Ursidae.

한국재래산양 신생자축의 혈액학치 변화에 관한 연구

조광현 ( Kwang-hyun Cho ),박용수 ( Yong-soo Park ),김성국 ( Seong-guk Kim ),어경연 ( Kyung-yeon Eo ),곽동미 ( Dongmi Kwak ),권오덕 ( Oh-deog Kwon ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.1

The present study was undertaken to clarify the changes in hematological values of Korean indigenous goats according to age. Blood samples were collected from 16 goats (5 females and 11 males) from birth up to the age of 6 weeks and the levels of various hematological values were analyzed. The body weight was increased from 2.54 kg at birth to 6.41 kg at 6 weeks age. The erythrocytes (RBC) counts and hemoglobin (Hb) values of goats were decreased at 2 weeks after birth, and increased gradually from 4 weeks after birth. The hematocrit (Ht) values were decreased until 4 weeks after birth, and increased at 6 weeks after birth. The mean cell volume (MCV) and mean cell hemoglobin (MCH) were decreased until 6 weeks after birth. The mean cell hemoglobin concentration (MCHC) was increased slightly from 2 weeks after birth. The red cell distribution width (RDW) was increased significantly at 2 weeks after birth, and decreased from 4 weeks after birth. The platelet (PLT) counts were increased significantly from 2 weeks after birth. The mean platelet volume (MPV) was decreased at 2 weeks after birth, and increased gradually from 4 weeks after birth. The total white blood cell (WBC) counts and the mean absolute numbers of neutrophil, lymphocyte, monocyte and eosinophil were increased from 2 weeks after birth. In conclusion, the data obtained from this study may be valuable as a standard for interpreting results of hematological analyses in Korean indigenous goats.

칡소 송아지의 생후 2개월간 혈액화학치 양상에 관한 연구

조광현 ( Kwang-hyun Cho ),박용수 ( Yong-soo Park ),김성국 ( Seong-guk Kim ),도재철 ( Jae-cheul Do ),어경연 ( Kyung-yeon Eo ),곽동미 ( Dongmi Kwak ),권오덕 ( Oh-deog Kwon ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.3

The present study was undertaken to clarify the changes in blood chemical values of indigenous Korean striped calves according to age. Blood samples were collected from 15 calves (8 females and 7 males) reared at the Gyeongsangbukdo Livestock Research Institute and the levels of various chemicals in se-rum were analyzed. The mean total protein, albumin and globulin concentration in calves increased from 2 weeks after birth, and the levels in females were higher than those in males at 8 weeks after birth. The mean glucose concentration increased significantly at 2 weeks after birth. The mean blood urea ni-trogen concentration increased from 2 weeks after birth, whereas the mean creatinine concentration de-creased significantly from 2 weeks after birth. The mean uric acid concentration increased gradually from 2 weeks after birth. The mean glutamic oxaloacetic transaminase and glutamic pyruvic trans-aminase concentration increased significantly from 2 weeks after birth. The mean total bilirubin concen-tration tended to decrease from 2 weeks after birth. The mean triglyceride, total cholesterol and high density lipoprotein cholesterol concentration increased significantly from 2 weeks after birth. In con-clusion, the data obtained from this study may be valuable as a standard for interpreting results of bio-chemical analyses in indigenous Korean striped calves.