Boophilus microplus 진드기 Nymph Stage Midgut의 DNase 특성

곽동미 ( Dong Mi Kwak ) 한국수의공중보건학회 2004 예방수의학회지 Vol.28 No.1

Babesia euqi ema-1 5` intergenic 뉴클레오타이드의 프로모터 위치 확인: I. PCR 증폭 및 제한효소지도

곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1

Babesia equi ema-1 5` intergenic(IG) nucleotide was PCR amplified and analyzed for restriction sites in order to identify a promoter region in this IG nucleotide sequence. B equi ema-1 5` IG specific primers identified a 1268 bp PCR product. The sequence had restriction sites for 34 restriction enzymes when analyzed by a computer program. Among them, 26 enzymes had only one restriction site, but the others had more than one sites. When four restriction enzymes(Bgll, HindⅢ, Kpnl and BamHl) were treated to digest the 1268 bp nucleotide, they had restriction sites as expected by the computer program. Information of restriction sites in the 1268 bp IG nucleotide will be applied to select restriction enzymes for cloning the IG nucleotide to a vector.

Neospora caninum Tachyzoites 분비액과 균질액에서의 DNase 활성 비교

곽동미 ( Dong Mi Kwak ) 한국수의공중보건학회 2004 예방수의학회지 Vol.28 No.2

Neospora caninum Tachyzoites에서의 66 kDa DNase 특성

곽동미 ( Dong Mi Kwak ) 한국수의공중보건학회 2004 예방수의학회지 Vol.28 No.2

Babesia bovis rap-1 및 B equi ema-1 intergenic 뉴클레오타이드에서 프로모터로 추정되는 위치 분석

곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1

Babesia bovis rap-1 and B equi ema-1 intergenic(IG) nucleotides were analyzed and compared for identifying putative promoter sites using computer programs. The reason to initiate this research was to determine if IG nucleotides of Babesia genes that are predicted to be involved in erythrocyte invasion have functions regulating gene transcription and translation, which can be applied to functional gene knockout. Four IG sequences used included BbIG5(B bovis rap-1 5` IG), BbIG3(B bovis rap-1 3` IG), BeIG5(B equi ema-1 5` IG) and BeIG3(B equi ema-1 3` IG). BbIG5 contained a putative promoter at nucleotide 197-246 with a predicted TATA-box and a transcription start site. BbIG3 had a putative promoter at nucleotide 270-320 with two predicted TATA-boxes and a transcription start site. BeIG3 had a putative promoter at nucleotide 155-205 with a predicted TATA-box and a transcription start site. Putative promoter sites in these three sequences mentioned above were identified with score cutoff 0.8, which means detection of about 40% recognized promoters with 0.1-0.4% false positives. In contrast, BeIG5 had a putative promoter at nucleotide 163-213 with score cutoff 0.8, but neither TATA-box nor transcription start site were recognized. However, BeIG5 had a putative promoter at nucleotide 388-438 with a predicted TATA-box and a transcription start site when score cutoff was decreased to 0.18, which means detection of about 70% recognized promoters with 2.2-5.3% false positives. These sequences with putative promoters can be tested if they have functions regulating gene transcription and translation.

경북 동부지역 소와 돼지에서의 톡소포자충 항체 조사

서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),이은미 ( Eun Mi Lee ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2

This study was conducted to determine the prevalence of antibodies to Toxoplasma gondii (TG) in cattle and pigs reared in eastern areas of Gyeongbuk province by ELISA. Among 368 sera collected from 119 cattle farms, 76 (20.7%) sera from 34 (28.6%) farms had antibodies to TG. Fifty (27.2%) out of 184 cattle in Uljin-gun and 26 (14.1%) out of 184 cattle in Yeongdeok-gun were positive. Pyeonghae (50.0%) in Uljingun and Dalsan (33.3%) in Yeongdeok-gun had the highest TG antibodies in cattle compared to other areas. Prevalence of TG antibodies in cattle was increased with age. Among 368 sera collected from 43 pig farms, 62 (16.8%) sera from 16 (37.2%) farms had antibodies to TG. Forty (21.7%) out of 184 pigs in Uljin-gun and 22 (12.0%) out of 184 pigs in Yeongdeok-gun were positive. Uljin and Puk (40.0%) in Uljin-gun and Yeonghae (33.3%) in Yeongdeok-gun had the highest TG antibodies in pigs compared to other areas. Prevalence of TG antibodies in sows was higher than that in fattening pigs. Seasonally, prevalence of TG antibodies in pigs was highest in summer (23.4%) and lowest in winter (12.5%). Based on these observations, data indicate that infection by the protozoan parasite TG is widely prevalent in cattle and pigs reared in eastern areas of Gyeongbuk province.

Characterization of Bovine Brucellosis in Korean Native Cattle by Means of Immunohistochemistry and Proteomics

Seong-Jun Jang(장성준),Sun-Hee Do(도선희),Mi-Ran Ki(기미란),Il-Hwa Hong(홍일화),Jin-Kyu Park(박진규),Yu-Jeong Cho(조유정),Sang-Joon Park(박상준),Tae-Hwan Kim(김태환),Dong-Mi Kwak(곽동미),Kyu-Shik Jeong(정규식) 한국생명과학회 2010 생명과학회지 Vol.20 No.2

본 연구는 브루셀라증 감염 소 혈청으로부터 분리한 항 브루셀라 면역글로블린 항체를 이용하여 조직 면역염색을 통한 브루셀라증 진단의 활용 가능성을 조사하고 병의 발생과 관련한 기능적 진단 마커를 개발하고자 하였다. Rose-Bengal test에 대해 양성 반응을 나타내어 브루셀라증으로 진단된 17개의 케이스와 음성 반응을 나타낸 19개의 대조군 케이스에 대해 조사를 실시하였다. 본 실험실에서 분리한 항 브루셀라 항체를 이용한 면역조직화학적 반응에서 간의 중심 소엽에 위치한 간세포의 세포질, 신장의 사구체 및 관 상피에서 강한 양성 반응을 나타내었다. 감염된 소의 간과 비감염 대조군의 간의 2차원 전기 영동법에 의한 단백질체를 비교 분석한 결과, 발현량이 대조군에 비해 유의적으로 증가한 5개의 단백질 스팟과 반대로 대조군에 비해 발현량이 현저히 감소한 5개의 단백질 스팟을 선별 하였다. 이 중 카탈라아제와 3-hydroxyacyl-CoA dehydrogenase의 발현 증가는 브루셀라증에 의한 장독 쇼크에 의한 산화적 스트레스 증가에 대한 방어적 반응으로 사료 되었다. 결론적으로, 항 브루셀라 면역글로불린 항체는 감염 조직의 감별 진단을 위한 좋은 진단 재료 임과, 더 나아가 단백질체학 분석을 통해 브루셀라증 진단 및 병리 연구를 위한 새로운 마커 단백질을 제시하였다. This study was conducted to examine the utilization of immunohistochemistry using the bovine anti-brucella immunoglobulin G (IgG) antibody in the diagnosis of brucellosis and to develop a functional biomarker relation for the progress of the disease. Anti-brucella IgG antibody was purified from the affected bovine serum using an affinity chromatography. We performed our investigation on 17 cases of brucellosis and 19 control cases with negative Rose-Bengal test results. Our purified anti-brucella IgG antibody showed a positive immunoreactivity in cytoplasmic hepatocytes of the centrilobular region, and glomeruli and tubular epithelium of the kidney. The protein pattern of the affected liver versus control was analyzed by two-dimensional electrophoresis, showing a different expression pattern of proteins between the two. Five protein spots were up-regulated and another were five down-regulated in the brucellosis liver. Significant upregulaton of catalase and 3-hydroxyacyl-CoA dehydrogenase might be due to a compensatory reaction in response to the endotoxic shock of brucella. In conclusion, the anti-brucella IgG antibody may be a good tool for discriminative diagnosis of the affected tissues and proteomics data suggest new target proteins underlying a possible pathogenic mechanism of brucellosis.

Sudden Death Caused by Clostridium perfringens Type D Enterotoxemia in Feedlot Cattle

Seong-Jun Jang(장성준),Sun-Hee Do(도선희),Mi-Ran Ki(기미란),Il-Hwa Hong(홍일화),Jin-Kyu Park(박진규),Yu-Jeong Cho(조유정),Ae Ri Ji,Se-Il Park,Sang-Joon Park(박상준),Tae-Hwan Kim(김태환),Dong-Mi Kwak(곽동미),Kyu-Shik Jeong(정규식) 한국생명과학회 2010 생명과학회지 Vol.20 No.5

비육우의 공장, 회장 및 결장의 특징적 괴사 출혈성 장염 증상은 폐사 증후군과 관련이 있는 것으로 인식되고 있다. 특히 젖먹이 송아지들이 폐사 증후군에 가장 영향을 받는데, 포유시 송아지에게 과식을 시키거나, 비위생적 초유 급유, 체온 하강 및 스트레스는 장독혈증 발생의 원인들이다. 2006년 7월 중 경북 영천 지역에서 축우의 폐사가 발생하였다. 식욕부진, 의기소침, 간헐적인 설사 및 경미한 호흡 곤란을 제외하고는 특별한 임상 증상은 없었으나, 조직학적 소견에 의하면, 심장, 신장, 간, 비장 및 임파선을 포함한 각종 기관에서 globular leukocytes의 침윤뿐 아니라 핵내 봉입체가 현저하게 나타났다. 비장과 림프조직에서는 림프 괴사와 림프구 괴사로 인한 ‘starry sky appearance’를 나타내었다. 소장 점막 하에서는 다량의 globular leukocytes와 호산 구의 침윤과 함께 호 염기성 세포 집단이 관찰되었다. 봉입체가 있는 소장 조직의 단면은 그램 염색에 대해 조직 화학적으로 양성반응을 나타내었다. 결론적으로, 비축우의 폐사 원인은 조직학적 및 조직화학적 분석 결과를 근거로 Clostridium perfringens D 형균에 의한 장독혈증으로 진단되었다. Sudden deaths have occurred in feedlot cattle with marked necro-hemorrhagic enteritis of the jejunum, ileum and colon. Suckling beef calves are the most frequently affected. Over-consumption of large amounts of milk, inadequate colostrum intake, chilling and stress are conducive to the development of enterotoxemia. Enterotoxemia caused by Clostridium perfringens type D mostly occurs following a sudden change of diet, particularly to feeds made richer in order to grow the cattle to market weight in feedlots. During July 2006, sudden deaths of cattle occurred in the Youngcheon regional area of Gyeongbuk province. There were no significant clinical signs except anorexia, depression, intermittent diarrhea and mild respiratory failure. Histological findings revealed a prominent intranuclear inclusion as well as infiltration of the globular leukocytes in various organs including the heart, kidneys, liver, spleen and lymph nodes. Spleen and lymphatic tissues showed lymphatic necrosis and a starry sky appearance. In the submucosa of the small intestines, basophilic aggregation was detected with massive infiltration of the globular leukocytes and eosinophils. Gram staining for the tissue sections containing inclusions of the small intestines revealed a positive histochemical reaction. Taken together, we suggest that Clostridium perfringens type D-induced enterotoxemia is determined to be the cause of sudden death of feedlot cattle.

Rat에서 Carboxymethyl Cellulose, Chondroitin 및 Carboxymethyl Chitosan의 자궁각 유착방지 효과의 비교

장환수 ( Hwan Soo Jang ),박상준 ( Sang Joon Park ),곽동미 ( Dong Mi Kwak ),장광호 ( Kwang Ho Jang ),권영삼 ( Young Sam Kwon ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.4

본 실험의 결과 3% carboxymethyl chitosan은 기존의 1% carboxymethyl cellulose와 유사하게 랫트의 자궁각에 유발시킨 유착 형성 과정에서 나타나는 염증세포 침윤, 콜라겐 형성 및 혈관신생을 억제하여 자궁각 유착 형성을 감소시키는 것으로 생각된다. This study was performed to compare the effects of carboxymethyl cellulose (CMCE), chondroitin sulfate (CHON), and carboxymethyl chitosan (CMCH) on preventing uterine horn adhesion. Adhesions were induced by suturing both the uterine serosa and peritoneum abrased until petechial bleeding occurred. Fourteen days later, adhesions were evaluated clinically and histopathologically. The tensile strength of adhesions formed significantly decreased in the CMCE and CMCH groups (p<0.05). The distance of adhesion site was significantly increased in the CMCE and CMCH groups comparing control group (p<0.05). In the CMCE, CHON, and CMCH groups, the inflammatory cell infiltration, collagen hyperplasia, and neovascularization were significantly lower than those of control group. Therefore, CMCH may be useful as an anti-adhesive agent in the prevention of uterine horn adhesion in rats.

국내 동물원에서 사육 중인 일본원숭이의 혈액상

권수완 ( Soo Whan Kown ),오석헌 ( Suk Hun Oh ),신남식 ( Nam Shik Shin ),송희종 ( Hee Jong Song ),곽동미 ( Dong Mi Kwak ),권오덕 ( Oh Deog Kwon ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.1

Hematological and serum biochemical values were assessed from 20 clinically healthy Japanese Macaques raised in Everland Zoological Gardens and compared to the International Species Information System (ISIS) reference range that is used internationally as standard for wildlife animals. Taking our standard on sexual maturation at age 4, tRBC values in Macaques under age 4 were significantly lower than those over age 4, but the Hb and PCV values were significantly higher. Compared to the ISIS standard, the tRBC values in Macaques under age 4 were significantly lower whereas the Hb and MCHC values were significantly higher. Moreover, in the samples of Macaques over age 4, the PCV and MCV values were significantly lower than the ISIS standard. On serum biochemistry values the creatinine and amylase values in the Macaques under age 4 were significantly lower than those over age 4. In comparison with the ISIS standard, the values of ALT, ALP, BUN, IP, Ca2+ and K+ in the Macaques under age 4 did have no significant difference. The values of TP, GGT, tBil, amylase, TG and UA were significantly higher than the ISIS standard, but the values of albumin, AST, glucose, creatinine, cholesterol, CPK, LDH, Na+ and Clwere significantly lower. In contrast, the values of TP, albumin, ALT, ALP, creatinine, cholesterol, amylase, TG, IP and Na+ in the Macaques over age 4 did have no significant difference, but the values of GGT, BUN, tBil, UA and Ca2+ were significantly higher, while the values of AST, glucose, CPK, LDH, K+ and Cl- were significantly lower. On the other hand, there was no significant difference in hematological and serum biochemical values between the groups of male and female.