보행용 MBT신발과 일반 신발 보행 시 에너지 소비량과 근 활성도의 비교 분석

윤범철,강세용,김애정,유민호,이미정,김난수,Yoon, Bum-Chul,Kang, Se-Yong,Kim, Yae-Jung,Yu, Min-Ho,Lee, Mi-Jung,Kim, Nan-Soo 대한물리치료과학회 2006 대한물리치료과학회지 Vol.13 No.3

This study was to find out whether MBT shoes were more effective than general sporting shoes on the kinematic physiology. We comparatively analyzed energy consumption, heart rates, rating of perceived exertion, lactic acid density in blood and muscle activity with EMG. The subjects were 10 regular students of K Univ.(5 male and 5 female). They wore the general sporting shoes for stage 1(group 1), the MBT walking shoes for stage 2(group2) under the same conditions. There was one week interval between stages. 1. There was no significant difference in the energy consumption and the heart rates between two groups. Group 1 showed a higher energy consumption and heart rates than Group 2 under the speed of 4.0 mph. On the other hand, Group 2 showed higher ones over the speed of 4.0 mph. 2. There was no significant difference in the rating of perceived exertion and lactic acid density in blood between two groups. But Group 2 showed a lower fatigue strength than Group 1. 3. Group 2 showed a higher muscle activity with EMG than group 1, especially at the speed of 2.0, 2.5, 3.0, 3.5 mph in quadratus lumborum muscle and at 4.0mph in tibialis anterior muscle. In conclusion, we are less tired in MBT shoes than in general sporting shoes. Also, we can dorsiflex better in the ankle joint, straighten the lumbar region well and walk erect in MBT shoes. Specially, when it walks slowly, it is favorable in an erection walk and when it walks quickly, we have advantage of obesity management.

기관지 천식 환자에서 천식 증상의 정도에 따른 $\beta_2$ 교감신경 수용체의 유전자 다형성

심재정,김제형,이승룡,권영환,이소라,이상엽,강세용,강용구,조재연,인광호,원남희,유세화,강경호,Shim, Jae-Jeong,Kim, Jei-Hyung,Lee, Seung-Yong,Kwan, Young-Hwan,Lee, So-Ra,Lee, Sang-Youb,Kang, Se-Yong,Kang, Yong-Koo,Cho, Jae-Youn,In, Kwang-Ho,Wo 대한결핵및호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.1

서 론: $\beta_2$ 교감신경 수용체 유전자에는 여러 종류의 다형성(polymorphism)가 존재하며, 천식 환자에서 $\beta_2$ 교감신경 수용체의 대표적인 변이는 $\beta_2$ 교감신경 수용체의 아미노산이 대치된 부분으로 Arg16-Gly, Gln27-Glu, Val34-Met 및 Thr164-Ile 등인 것으로 알려져 있다. 지속적인 $\beta_2$ 교감신경 유도체의 자극에 대하여 세포표면으로 부터 세포내의 전달과정이 둔화되어 점차 세포전달이 없어질 수도 있는 desensitization 또는 수용체와 수가 감소하는 downregulation이 존재하는 것으로 알려져 있다. 천식환자에서 $\beta_2$ 교감신경 수용체의 desensitization 또는 downregulation 뿐만 아니라 천식 표현형과 $\beta_2$ 교감신경 수용체 유전자 다형성의 상관 관계에 대한 연구가 이루어지고 있으나 논란이 많다. 이에 본 연구는 기관지 천식환자에서 $\beta_2$ 교감신경 수용체의 가장 흔한 16, 27, 34 및 164 의 아미노산에 해당하는 유전자의 다형성을 MASA (Mutated Allele Specific Amplification)법으로 시행하여 각각의 다형성의 발생 빈도와 천식의 심한 정도와 연관이 있는 가를 확인하였다. 대상 및 방법: 대상 환자는 천식 환자 103명이었으며, 이중 남자는 54명, 여자는 49명으로 평균 연령은 46.6세 (19~80세)였고 이환 기간은 4.7년이었다. 대상 환자는 경미하고 간헐적 증상을 보인 30명, 지속적인 경미한 천식 환자는 32명으로 경미한 천식은 모두 62명이었으며, 중등증의 천식 증상은 17명 및 중종의 천식증상을 보인 환자는 24명이었다. 이중 1년 중에 6개월 이상 전신적 스테로이드를 투여하는 환자는 39명이었으며, 투약 중에도 야간 발작이나 야간 기침이 발생되었던 환자는 44명이었다. 대상 환자로부터 10cc의 전혈구를 체취 하여 분리된 림파구에서 분리된 DNA를 이용하여 MASA 방법으로 $\beta_2$ 교감신경 수용체 16번, 27번, 34번 및 164번째 아미노산의 다형성을 검색하였고, 천식의 심한 정도 따른 $\beta_2$ 교감신경 수용체 유전자의 다형성의 분포와 야간 천식의 발작이나 증상의 유무에 따른 $\beta_2$ 교감신경 수용체 유전자의 다형성의 분포를 확인하였다. 결 과: 16 번째 Arginine이 Glycine으로 변이는 heterozygous 변이가 67명, homozygous 변이가 13명으로 heterozygous 변이가 65.1%로 가장 많았다. 27번째 Glutamine이 Glutamate로 변이는 heterozygous만 11명으로 10.7%였으며, 34번째 Valine이 Methionine으로 변이를 일으키는 100번째 핵산의 경우도 heterozygous만 6명으로 5.8%였다. 27 번째와 34번째 아미노산의 변이를 일으키는 homozygous 변이와 164번째 아미노산의 변이는 대상 환자 중에는 없었다. 천식 증상의 심한 정도를 경종 및 중등증, 중중으로 2 구분하여 $\beta_2$ 교감신경 수용체 다형성의 발생빈도를 관찰한 결과 중증의 천식환자에서 16번째 아미노산의 변이의 빈도는 많았으나 (p=0.015), 27번, 34번 및 164번째의 아미노산의 변이는 천식 증상의 정도와는 연관성이 없었다. 야간 천식 증상의 유무에 따른 $\beta_2$ 교감신경 수용체 다형성은 16, 27, 34 및 164번째 아미노산의 핵산의 변이와 연관성이 없었다. 결 론: 이상의 결과로 기관지 천식 환자에서 $\beta_2$ 교감신경 수용체 다형성은 Arg 16, Gln 27 및 Val 34의 변이가 존재하고, Arg 16이 가장 많았으며, Thr 164는 없었다. 기관지 천식 환자에서 증상이 심한 중증 천식은 $\beta_2$ 교감 신경 수용체의 다형성중 Arg 16의 변이는 중증 천 Background: Genetic and environmental factors are known to affect the incidence and severity of asthma. Stimulation of $\beta_2$-Adrenergic Receptor ($\beta_2$AR) results in smooth muscle relaxation, leading to decrease in resistance of airflow. The gene encoding the $\beta_2$AR has recently been seguenced. The $\beta_2$AR genotype at the polymorphic loci of codons 16, 27, 34, and 164 was known to cause changes in the amino acids. The relationships between the structure of the $\beta_2$AR and its functions are being elucidated. Purpose : The gene encoding the $\beta_2$AR was carried out to assess the frequency of polymorphisms in bronchial asthma, to determine wheather these polymorphisms have any relation to the severity, or nocturnal symptoms in bronchial asthma. Methods: The subjects studied were 103 patients with bronchial asthma, which consisted of 30 mild episodic, 32 mild persistent, 17 moderate, and 24 severe asthma patients. The polymorphisms of the $\beta_2$AR gene were detected by mutated allele specific amplification (MASA) method at the codons 16,27,34, and 164. Results: The most frequent polymorphism was arginine 16 to glycine. The other two polymorphisms, valine 34 to methionine and glutamine 27 to glutamic acid occured in 11 and 6 patients respectively. The polymorphism of threonine 164 to isoleucine was not found in our enrolled patients. The homozygous polymorphism of $\beta_2$AR gene was found in only arginine 16 to glycine (12.6%). The heterozygous polymorphisms of $\beta_2$AR gene were in arginine 16 to glycine, valine 34 to methionine, and glutamine 27 to glutamic acid, as 65.1 %,10.7%, and 5.8% respectively in asthma patients. The presence of agrginine 16 to glycine heterozygous or/and homozygous polymorphism was associated in severe asthma (p=0.015), but there was no association between the other three polymorphisms and the severity of asthma. The frequency of the $\beta_2$AR gene polymorphisms was no relation in nocturnal asthma as compared with non-nocturnal asthma. Conclusion: The arginine 16 to glycine polymorphism of the $\beta_2$AR gene is the most frequently found in asthma patients and association with severe asthma. But there was no association between the polymorphism of the $\beta_2$AR gene and nocturnal asthma.

연구보고 : 천식 환자의 기관지 조직에서 Eotaxin mRNA 발현에 관한 연구

인광호 ( Kwang Ho In ),조재연 ( Jae Yun Cho ),강세용 ( Sae Yong Kang ),이상엽 ( Sang Youb Lee ),심재정 ( Jae Jeong Shim ),강경호 ( Kyung Ho Kang ),유세화 ( Se Hwa Yoo ),나영순 ( Young Soon Na ),김한겸 ( Han Gyum Kim ) 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.4

외부 방사선 치료 조사후 재발한 기관지내 악성종양에서 고선량율 근접조사치료(High Dose Rate Brachytherapy)의 효과

조재연 ( Jae Youn Cho ),인광호 ( Kwang Ho In ),서정경 ( Jung Kyung Suh ),강세용 ( Sea Yong Kang ),심재정 ( Jae Jeong Shim ),강경호 ( Kyung Ho Kang ),김광택 ( Kwang Taek Kim ),김철용 ( Cheol Yong Kim ),유세화 ( Se Hwa Yoo ) 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.1

기관지 천식 환자에서 천식 증상의 정도에 따른 β2 교감신경 수용체의 유전자 다형성

심재정 ( Jae Jeong Shim ),김제형 ( Jei Hyung Kim ),이승룡 ( Seung Yong Lee ),권영환 ( Young Hwan Kwan ),이소라 ( So Ra Lee ),이상엽 ( Sang Youb Lee ),강세용 ( Se Yong Kang ),강용구 ( Yong Koo Kang ),조재연 ( Jae Youn Cho ),인광호 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.1

반복성 기흉(氣胸)을 유발한 원발성 폐(肺) 조직구증-X

강경호,유세화,인광호,구양서,강세용 대한내과학회 1992 대한내과학회지 Vol.43 No.1

Pulmonary histiocytosis X can be associated with disseminated histiocytosis X, but isolated case in the lung parenchyme is rare. We report a case of primary pulmonary histiocytosis X with recurrent pneumothoraces. A 36-year-old man was referred because of recurrent pneumothorax. He had been suffered from dry cough and exertional dyspnea for 4 years. He was a heavy smoker. Plain chest X-ray and chest CT scan revealed thin-walled cysts of various sizes in both lungs. Open lung biopsy was performed. Light microscopic examination revealed proliferation arid infiltration of Langerhans'cells. Immunohistochemically, Langerhans`cells showed strong cytoplasmic staining with S-100 protein and electronmicroscopic examination showed Birbeck granules in Langerhans`cell. He was treated with bleb resection and pleurodesis, but pneumothorax recurred 1 month later.

Ceftazidime의 임상 효과

최윤상,강세용,우흥정,신상원,김우주,최석주,김민자,박승철 대한화학요법학회 1990 대한화학요법학회지 Vol.8 No.2

Ceftazidime is a '3rd grenration' cephalosporin which possesses broad spectrum activity and high activity against Pseudomonas aeruginosa. To evaluate the clinical effecacy of ceftazidime, the drug was administered parenterally to a total 26 patients with various bacterial infection; 15 with urinary tract infection, 8 with respiratory tract infection, 2 with acute cholecystitis, and 1 with cellulitis. The results were as follows: 1. The clinical response was satisfactory in 15(100%) of 15 patients with urinary tract infection, 6(75%) of 10 with pneumonia, 1(50%) of Overall, ceftazidime was effetive in 88.5%(23/26) of all patients treated. 2. In 19 bacteriologically proven cases, 18 orgainsms except one were eradicated during treatment. 3. Adverse effects were observed in 3(11.5%) of 26 patients; skin rash (1 patient) and nausea(2 patients).

당뇨병 환자에서 말초혈액 중성구의 Superoxide Anion 생성능에 대한 연구

신상원,유재명,강세용,우흥정,김우주,박승철 대한감염학회 1992 감염 Vol.24 No.4

It is generally believed that diabetics have increased susceptibility to infections, and have worse prognosis than nondiabetic population. But there is no evidence that immunologic competence is impaired in patients with diabetes. This study was designed to investigate the association of the superoxide anion production by peripheral blood neutrophild with the infections and other complications in diabetic patient. Superoxide anion production by peripheral neutrophils in 19 healthy subjects, and 40 diabetic patients(15 with infection and 25 without infection) were measured. The superoxide anion production, measured by spectrophotometric assay was expressed in absorbance at 550nm. The results were as follows: 1) After stimulation with ?? FMLP(N-formyl methionyl leucyl phenylalanine), the superoxide production by neutrophil was higher in normal control group (17.14±3.54 nmole/30 min/1×?? neutrophils) than diabetic patient group of diabetic patients with group(14.58±3.55 nmol/30 min/1×?? neutrophils). 2) Superoxide anion production was lower in the group of diabetic patients with infections(14.58±3.55 nmole/30 min/1×?? neutrophils) than the normal control group(17.14±3.54 nmole/30 min/1×?? neutrophils). 3) Superoxide anion production was lower in the group of diabetic patients with complication(14.13±3.33 nmol/30 min/1×?? neutrophils) than the group of diabetic patients without complication(17.05±4.45 nmol/30 min/1×?? neutrophils). In conclusion, the superoxide anion production defect in diabetic patient have associated with development of infections. And superoxide anion production may appear to have influence on the development of diabetic complication.

건강한 남자에서 하지 손상후 발병한 피부 및 연조직 모균증 1예

김우주,신상원,우흥정,김민자,강세용,홍영규,박승철,최윤상 대한감염학회 1991 감염 Vol.23 No.1

The Mucorales are ubiquitous fungi. Disease in healthy persons is very rare. In most cases, mucormycosis occurred in patients with diabetes, leukemia or lymphoma, and impaired host defense mechanisms. Frequent sites of involvement are rhinocerebral, orbital, central nervous system, pulmonary, skin and soft tissue, gastrointestinal tract, occasionally disseminated infection occur. The disease progresses and results fatal outcome without appropriate management. In patient with cutaneous mucormycosis, systemic administration of amphotericin-B and repeated surgical debridement of necrotic tissue are satisfactory. Occasionally aggressive infection necessitated a curative amputation. We recently encountered a 34-year-old healthy male who was managed with intravenous amphotericin-B and amputation under the diagnosis of mucormycosis of skin and soft tissue.

염색체 DNA 제한효소 절편분석 및 Southern hybridization 을 이용한 병원 칸디다 감염증의 역학적 연구

김우주,박승철,정희진,유철웅,강세용,우흥정 대한내과학회 1999 대한내과학회지 Vol.57 No.2

$quot;Background : Candida species are not only the leading cause of nosocomial fungal infections but also the important cause of infections in the immunocompromised hosts. Epidemiologic study of nosocomial candidiasis has been difficult because of the lack of a reliable typing system. We performed molecular epidemiologic study by using RFA and Southem hybridization for typing of candida isolates from patiients. Methods : A total of 27 candida isolates from 19 immunocompromised patients were studied. Morphotyping and biotyping were done by germ tube test and API 20C system, respectively. Candidial chromosomal DNA was extracted, digested with EcoRI, HindalII and RFA was done. Southem hybridization of chromosomal DNA was also done with digoxigen-labelled Candial albicans-specific DNA probe. Results : The time-period of development of oral candidiasis after admission was 5-14 days (mean : 8 days). C.albicans was the most common species (19), followed by C tropiadis (2), C glabn#zta (2), C.paratropicalis (2), and C parapsilosis (1). The subtypes of Candida species by RFA of chromosomal DNA were C. albieans, 12 types , C tropicalis, 2 types, C glabrata, 2 types ; C.parapsilasis, 1 type ; C. paratropicalis, 1 type. For 7 (87.5%) of 8 patients, RFA pattern of one isolate was identical to that of the other isolates. Conclusion : RFA of candidial chromosomal DNA results were obtainable within days. RFA showed high reproducibility, typeability and good discrimination power between isolates, provided a robust system that may be used rapidly to identify outbreaks of nosocomial candidiasis.$quot;