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몽골 유래 Brucella melitensis 동정 및 특이 SNP를 이용한 real-time PCR법에 의한 진단 평가
강성일,김지연,김숙미,이진주,성소라,김연희,정석찬,허문,Kang, Sung-Il,Kim, Ji-Yeon,Kim, Suk Mi,Lee, Jin Ju,Sung, So-Ra,Kim, Yeon-Hee,Jung, Suk Chan,Her, Moon 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.2
A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.
IEEE-1394 버스의 가용 등시성 대역폭을 활용하기 위한 대역폭 훔치기와 ABR-1394 전송 모드
강성일(Kang Sung Il),이윤직(Lee Yoon Jick),이흥규(Lee Heung Kyu),강성봉(Kang Sung Bong) 한국정보처리학회 1999 정보처리학회논문지 Vol.6 No.6
The IEEE-1394 High Performance Serial Bus provides the real-time isochronous transport with capability of bandwidth reservation, however, there is a problem that isochronous bandwidth is used inefficiently on transmitting multimedia data with variable rate via the isochronous transport. To improve utilization of isochronous bandwidth, this paper proposes a bandwidth stealing technique to be able to recognize available isochronous bandwidth without any effects on current isochronous transmissions with reserved bandwidths, standard nodes and extended nodes with the bandwidth stealing technique can be connected together on the same bus. The ABR-1394 transport enables applications to utilize available isochronous bandwidth efficiently. In the respect of the utilization of available bandwidth, ABR-1394 is similar to the ABR service of ATM, however, it is different in that an ABR-1394 node itself can do flow control independently without any help from the network.
제주 음식관광객 프로파일 비교연구 : 맛집 방문객과 로컬푸드 레스토랑 방문객을 중심으로
강성일(Kang, Sung-Il),강숙영(Kang, Sook-Young) 한국해양관광학회 2020 해양관광학연구 Vol.13 No.3
The aim of this paper is to explore the profile of food tourists whose destination is Jeju. Fieldwork was carried out among a serises of restaurant establishments in Jeju. A demand survey was conducted in 20 particular restaurants that were selected among those offering local dishes and regularly visited by tourists. This study contributes regional balanced local food and restaurant industry development by proposing differentiated food tourists in Jeju region and contributes to promote related studies by suggesting advanced skills in establishing policies on Jeju tourist food industry and food tourism.
강성일(Sung Il Kang),김종우(Jong Woo Kim),김승기(Seung Ki Kim),정철운(Chul Woon Chung),권성원(Sung Won Kwon),안대호(Dae Ho Ahn),이경포(Kyong Po Lee),이경식(Kyong Sik Lee) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.6
Purpose: Endometriosis is the heterotropic occurrence of island of endometrial glands and stroma outside of uterus. It common occurs in pelvic cavity, but rarely at the ectopic area of extra-pelvic cavity. The aim of this study was to evaluate the clinical characteristics of extrapelvic endometriosis in order to help diagnosis and treatment. Methods: Between January 2001 and June 2007, 22 patients who underwent surgery and was diagnosed as ectopic endometriosis of extra-pelvic cavity at Bundang CHA hospital were retrospectively reviewed with medical records and a telephone interview. Results: All cases were women in their reproductive age, with a median age of 33 years (range 24~49 years). 17 of 22 cases were endometriosis in the scarring tissue of prior caesarean section wound on abdomen. 1 case in vaginal orifice on episiotomy wound of perineum, 1 case in femoral ring area and 3 cases in appendix. All patients except endometriosis of appendix, were presented with mass, pain and symptoms almost associated with menstruation. 18 of 22 cases were suspected of having extra-pelvic endometriosis due to their specific clinical features, 4 cases were suspected of hernia and acute appendicitis. All patients were treated with surgery. None has revisited to clinics due to recurrence. Conclusion: Women who have mass or lump in surgical scar and symptoms associated with menstruation period should be suspected of having endometriosis. Endometriosis of surgical scaring tissue should be treated by wide local excision to prevent local recurrence.
복강경하 위소매절제술 후 합병증의 조기 발견에 있어 임상 지표의 중요성
강성일(Sung Il Kang),한상문(Sang Moon Han),김원우(Won Woo Kim),이경포(Kyung Po Lee),이경식(Kyung Sik Lee) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.5
Purpose: The significant drastic complications of performing laparoscopic sleeve gastrectomy (LSG) in morbidly obese patients are gastric staple line leakage and bleeding. The aim of our study is to evaluate the efficacy of the clinical data for detecting postoperative complications after LSG. Methods: The study enrolled 150 consecutive patients who underwent LSG from January 2003 to July 2006. When abnormal data (heart rate ≥110/min, or temperature ≥37.5℃) was detected on postoperative day 1, then laboratory tests (blood, urine, chest X-ray and abdominal sonogram) and water soluble gastrografin UGIS were performed to detect the postoperative complications after LSG (group A). The patients who had normal postoperative clinical data (group B) were compared with group A. Results: Of the 150 patients who underwent LSG, 9 patients (6%) had postoperative complications. Two patients had major complications: 1 case of leakage (0.6%) and 1 case of delayed bleeding (0.6%), and 4 patients had minor complications in group A. But no major complications were detected in group B (P<0.01). The heart rate and body temperature in group A were significantly faster and higher than those in group B (P<0.01). Especially, 2 patients who had major complications had severe tachycardia over 120 beats per minute (bpm). However, when the body temperature of a patient was elevated, there was no high grade fever in all the cases (>39℃). Conclusion: Evidence of tachycardia or a high body temperature may be useful to detect major complications after LSG. We also recommend performing laboratory test and UGIS when clinically indicated.
Alkaline protease inhibitor를 생산하는 해양유래 방선균의 탐색 및 동정
강성일(Sung-Il Kang),공재열(Jai-Yul Kong),최영준(Yeung Joon Choi),김민용(Min-Yong Kim),손홍주(Hong-Joo Son) 한국생명과학회 2008 생명과학회지 Vol.18 No.4
Alkaline protease는 식품 가공 공정에 있어 가공 원료의 선도 저하를 초래하거나 맛살류 제품 원료인 어류의 근육조직을 파괴하여 gel 구조를 파괴시키는 것으로 알려져 있다. 본 연구는 해양으로부터 alkaline protease에 대한 저해력을 가지는 방선균들을 분리하여 그 중 저해력이 가장 높은 C12 균주를 최종 선정하였다. 본 균주의 형태학적, 배양적 및 생리학적 특성을 조사한 결과, 포자의 크기는 2.0 ㎛로 외형은 원통형이고, 편모가 없으며, 포자형태는 smooth하였다. ISP 9 배지를 제외한 대부분의 배지에서 잘 성장하였다. 또한 15~50oC에서 잘 성장하였으며, 9% (w/v) NaCl이 포함된 배지에서도 성장하는 것으로 확인되었다. Gram 양성, citrate 음성, catalase 양성이었으며, melanin 색소를 생성하지 않았다. Starch, casein 및 gelatin 분해능이 있었으며, glucose, galactose, maltose, lactose, fructose 및 mannse 등은 잘 이용하였지만, sorbitol과 sucrose는 이용하지 않았다. 이러한 특성을 토대로 본 균주는 Streptomyces sp.로 확인되었다. 보다 정확한 균주 동정을 위하여 16S rDNA 염기서열 분석을 수행하였으며, 그 결과 C12 균주는 S. thermocarboxydus와 계통진화학적으로 가장 유연관계가 높았다. In this study, we screened and identified the bacterial strain showing high alkaline protease inhibitor activity from marine environment. Nine bacterial strains with alkaline protease inhibitor activity were isolated from marine sediments. Among them, strain C12 had the highest alkaline protease inhibitor activity and was selected for further taxonomical study. On the basis of morphological and physiological characteristics, strain C12 was identified as the genus Streptomyces. A phylogenetic analysis of the 16S rDNA showed that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces thermocarboxydus. Morphological characteristics showed cylindrical spore chain and smooth spore surface by scanning electron microscope. Strain C12 was grown on all media except for ISP 9 agar. This strain could be grown in the medium containing up to 9% NaCl.