보문 : 항고혈압성 안지오텐신 전환효소 저해제를 생산하는 새로운 효모의 선별 및 저해물질 최적 생산조건

강민구 ( Min Gu Kang ),김하근 ( Ha Kun Kim ),이성훈 ( Sung Hun Yi ),임성일 ( Sung Il Lim ),이종수 ( Jong Soo Lee ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

Forty eight strains of yeast were cultured in potato dextorse(PD) broth at 30˚C for 24 hr and centrifuged with 12,000 rpm for 20 min. After concentrated the cultures, antihypertensive angiotensin I-converting enzyme(ACE) inhibitory activities of its concentrates were investigated. Among them, the concentrates from Saccharomyces cerevisiae Y183-3 showed the highest ACE inhibitory activity of 71.8%. The ACE inhibitor from Saccharomyces cerevisiae Y183-3 was maximally produced when Saccharomyces cerevisiae Y183-3 cultured in PD broth at 30˚C for 36 hr.

Meta-analysis of the Efficacy and Safety of Grazoprevir and Elbasvir for the Treatment of Hepatitis C Virus Infection

강민구,강민정,지은희,유봉규,Kang, Min Gu,Kang, Min Jung,Ji, Eunhee,Yoo, Bong Kyu Korean College Of Clinical Pharmacy 2017 한국임상약학회지 Vol.27 No.3

Background: Recently, a fixed combination of grazoprevir and elbasvir (GE) has been introduced to the arsenal of chemotherapeutics to fight against this virus. The study aimed to provide information on the efficacy and safety of GE for the treatment of HCV infection by performing a meta-analysis of literature data. Methods: PubMed and EMBASE database searches were conducted. Among the literature retrieved, pivotal Phase III clinical studies were analyzed. Statistical analysis of the data was performed by RevMan. Results: Four pivotal Phase III clinical studies compared the efficacy and safety of GE. When HCV patients were treated with GE for 12 weeks, the sustained virologic response, defined as the viral RNA level below the lower limit of quantification at 12 weeks after the cessation of therapy (SVR12), was 94.7%. The clinical advantage of GE involves its use by patients with cirrhosis and/or renal failure without dose adjustment. If the genotype (GT) of the causative virus was GT1a with NS5A polymorphism or GT4 with resistance to peginterferon/ribavirin, treatment with GE plus ribavirin for 16 weeks resulted in a better outcome compared to treatment with GE alone for 12 weeks. Adverse events reported during the four clinical studies were 71.09% in the GE arms and it was 76.61% in the non-GE arms, with the most frequent events being mild central nervous system symptoms. Conclusion: GE was generally safe and effective for the treatment of HCV infection. However, since HCV mutates very rapidly and becomes resistant to antiviral agents, long-term monitoring should be mandatory.

실리콘 이종 접합 태양 전지 특성에 대한 ZnO:Al과 비정질 실리콘 계면 반응의 영향

강민구,탁성주,이종한,김찬석,정대영,이정철,윤경훈,김동환,Kang, Min-Gu,Tark, Sung-Ju,Lee, Jong-Han,Kim, Chan-Seok,Jung, Dae-Young,Lee, Jung-Chul,Yoon, Kyung-Hoon,Kim, Dong-Hwan 한국재료학회 2011 한국재료학회지 Vol.21 No.2

Silicon heterojunction solar cells have been studied by many research groups. In this work, silicon heterojunction solar cells having a simple structure of Ag/ZnO:Al/n type a-Si:H/p type c-Si/Al were fabricated. Samples were fabricated to investigate the effect of transparent conductive oxide growth conditions on the interface between ZnO:Al layer and a-Si:H layer. One sample was deposited by ZnO:Al at low working pressure. The other sample was deposited by ZnO:Al at alternating high working pressure and low working pressure. Electrical properties and chemical properties were investigated by light I-V characteristics and AES method, respectively. The light I-V characteristics showed better efficiency on sample deposited by ZnO:Al by alternating high working pressure and low working pressure. Atomic concentrations and relative oxidation states of Si, O, and Zn were analyzed by AES method. For poor efficiency samples, Si was diffused into ZnO:Al layer and O was diffused at the interface of ZnO:Al and Si. Differentiated O KLL spectra, Zn LMM spectra, and Si KLL spectra were used for interface reaction and oxidation state. According to AES spectra, sample deposited by high working pressure was effective at reducing the interface reaction and the Si diffusion. Consequently, the efficiency was improved by suppressing the SiOx formation at the interface.

통합 프린지 해석 시스템 개발 : 심한 잡음을 포함하는 간섭 이미지 해석용

강민구,주원종,차동진,강보선,Kang, Min-Gu,Joo, Won-Jong,Cha, Dong-Jin,Kang, Bo-Sun 대한기계학회 2003 大韓機械學會論文集B Vol.27 No.11

A new window-based, user-friendly fringe analysis system is developed, especially for analyzing noisy interferograms. The system integrates three major techniques, that is fringe tracking, Phase shifting, and Fourier transform, into a single shell by employing a unified procedure. Since the system is made in a modular fashion and all processing modules can be shared for any technique, a user can select necessary modules and easily edit the applying order of them based on the user's analysis strategy, which should be changed depending on the noise level of the image. The system provides a high-level GUI and a variety of image handling tools and therefore users can easily access the system and produce the optimal results without giving up in the middle of a process even for severely noise-contaminated interferometric images.

2006년 태풍 EWINIAR 내습시 남강 지류 하천들의 홍수 피해와 향후 피해 저감 대책

강민구,박승우,박종윤,이철규,Kang, Min-Gu,Park, Seung-Woo,Park, Jong-Yun,Lee, Cheol-Gyu 한국수자원학회 2006 물과 미래(한국수자원학회지) Vol.39 No.12

Effect of dietary supplementation of quercetin on antioxidant activity and meat quality of beef cattle

강민구,김현주,장애라,감동근,윤관식,조철훈,Kang, Min-Gu,Kim, Hyun-Joo,Jang, Ae-Ra,Gam, Dong-Keun,Yun, Gwan-Sik,Jo, Cheo-Run Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.1

This study was carried out to investigate the effects of dietary supplementation of quercetin ($Kocetin^{TM}$, QR) on antioxidative activity and meat quality of beef cattle (Holstein-Friesian). Beef cattle were divided into 3 groups; dietary supplementation of QR at 21 (n=4) and 42 ppm (n=3), and non-supplemented control (n=4). The QR comprised of 10% of quercetin. After slaughtering the beef cattle, loins were obtained and analyzed. Dietary supplementation of QR at 42 ppm showed significantly higher final pH of loin but did not affect the water holding capacity, drip loss, cooking loss, surface color, total phenolics content, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Dietary QR showed no difference in both 2-thiobarbituric acid reactive substances and volatile basic nitrogen values. Textural characteristic results also showed no difference, except for cohesiveness. Cohesiveness was significantly higher in loin from beef cattle treated by dietary QR at 42 ppm when compared to control. Results suggest that dietary QR, which has only 10% of quercetin is not sufficient to have positive biochemical effects on beef meat quality.

Annual Occurrence Patterns and Three Dimensional Distribution of Mushroom Flies in Pleurotus ostreatus Cultivation Farms

강민구,김승한,최치완,헨리디즈니,권오석,Kang, Min-Gu,Kim, Seung-Han,Choi, Chee-Wan,Disney, Henry L,Kwon, Oh-Seok Korean Society of Applied Entomology 2017 한국응용곤충학회지 Vol.56 No.2

최근 느타리버섯은 연중재배가 보편화되고 있으며, 재배량도 급격히 증가하고 있는 추세이다. 경북지역 느타리버섯 재배사에서 3년에 걸쳐 버섯파리 발생밀도를 조사한 결과 긴수염버섯파리와 버섯벼룩파리가 가장 우점하여 발생하였다. 일반적으로 균사배양기간에는 버섯파리 밀도가 모든 농가에서 낮았지만 1주기 수확후에는 급격히 증가하는 경향을 보였다. 하지만, 여름철에는 배양기간임에도 불구하고 많은 버섯파리가 관찰되었다. 이 현상은 연중재배체계의 보편화로 인해 계절적 선택재배와 달리 버섯파리가 재배사에서 안전하게 월동할 수 있는 서식처를 제공받았기 때문이다. 버섯파리의 생태는 버섯재배환경에 따라 달라질 수 있다. 계절적 선택재배농가와 연중재배농가의 버섯파리 발생패턴 차이를 통해 휴경이 버섯파리 발생밀도 감소에 중요한 역할을 한다는 것을 확인하였다. 버섯재배사 내부의 버섯파리 입체발생분포 조사를 통해 긴수염버섯파리보다 버섯벼룩파리가 빛에 더 민감한 종임을 확인하였다. 또한 2종의 입체적 발생양상은 매우 차이가 있는 것을 확인하였다. Among flies inside mushroom growing rooms in three farms, Lycoriella ingenua and Megaselia tamilnaduensis were the most common and dominant species during Pleurotus ostreatus cultivation in Korea. In the past, generally, during the incubation period, a low density of mushroom flies was observed in all farms. After the first harvest, mushroom flies density tended to increase sharply. However, many mushroom flies were observed in the summer, despite that season corresponds to the incubation period. This is because annual cultivation systems provide a safe overwintering place compared to seasonal selective ones. The ecology of mushroom flies varies greatly according to the cultivation system. We confirmed that a fallow period reduced the density of mushroom flies. From a survey of the three dimensional distribution of mushroom flies in a growing room, we observed that M. tamilnaduensis showed more positive phototaxis and a higher variation per point of capture than that by L. ingenua. Through this study, two mushroom fly species were identified in the survey farms, with markedly different three dimensional distribution patterns.

심바스타틴이 인체망막색소상피세포에서 카탈라아제 발현에 미치는 영향

강민구,이소영,진희승,Min Gu Kang,So Young Lee,Hee Seung Chin 대한안과학회 2014 대한안과학회지 Vol.55 No.10

Purpose: To evaluate the effects of simvastatin on the catalase expression in human retinal pigment epithelium. Methods: Retinal pigment epithelial (RPE) cells were incubated for 6 hours and 24 hours with various concentrations of simvastatin. In addition, RPE cells were incubated with 200 μM of H<SUB>2</SUB>O<SUB>2 </SUB>and various concentrations of simvastatin. After incubation, real-time polymerase chain reaction (PCR) was performed to examine the catalase messenger ribonucleic acid (mRNA) expression and a catalase assay was performed to examine the catalase activity in RPE. Intracellular reactive oxygen species (ROS) was measured using a fluorescence activated cell sorter (FACS). Results: Simvastatin increased the amount of catalase mRNA and catalase activity at 10 μM in RPE cells. Under oxidative stress (200 μM of H<SUB>2</SUB>O<SUB>2</SUB>), 2.5 μM of simvastatin increased the catalase mRNA expression and 5 μM of simvastatin increased catalase activity in RPE cells. In addition, simvastatin reduced free radical formation but this effect was diminished in the presence of an irreversible catalase inhibitor, 3-amino-1,2,4-triazole (3-AT). Conclusions: Simvastatin exhibits anti-oxidative effects by inducing the catalase expression in human RPE cells. This anti-oxidative effect may be beneficial for preventing age-related macular degeneration induced by oxidative stress. J Korean Ophthalmol Soc 2014;55(10):1535-1542

MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus

강민구,차현정,송선희,박진우,서조영,이재목,Kang, Min-Gu,Cha, Hyun-Jeong,Song, Sun-Hee,Park, Jin-Woo,Suh, Jo-Young,Lee, Jae-Mok The Korean Academy of Periodontoloy 2005 Journal of Periodontal & Implant Science Vol.35 No.3

치주질환은 치주지지 조직의 상실로 특징 지워지는 감염성 질환으로, 지주조직의 세포외 기질(특히 교원질)이 주요 degradation target이 된다. 현재로서는 MMP family가 주역을 담당하고 있음이 밝혀졌는데, 구강 조직에서 가장 광범위하게 분포하는 것이 MMP-2, MMP-8 이다. 치주조직에서 MMP-2는 주로 결합조직내 섬유아세포와 육아조직에서, 발현되며 , basement membrane의 integrity와 접합상피 부착에 영향을 줄 수 있고, MMP-8은 neutrophils에서 발현되어 다양한 염증세포와 염증진행에 영향을 주는 것으로 알려져 있다. 치주질환에서 MMP-2, MMP-9의 발현 증가는 이미 치은 열구액을 분석한 여러 연구에서 보고 되어 그 발현 수준이 치주질환 진단의 좋은 indicator가 될 수 있음이 시사되었다. 치주질환에서 MMP-2, MMP-8의 발현이 증가하는 것은 주로 P. gingivalis, IL-1과 연계되어 그 기전이 추정되어 왔는데, 이는 2형 당뇨병이 치주 질환의 심도에 영향을 미치는 기전과 많은 부분을 공유한다. 따라서 본 실험에서는 2형 당뇨병 환자와 비당뇨자에서 만성 치주염 부위의 치은, 건강한 치은에서 염증 매개체 중 하나인 MMP-2, MMP-8의 발현에 대해 비교 분석함으로써 염증, 혈당이 미치는 영향을 밝히고 2형 당뇨환자의 만성치주염 치은조직에서 치주조직 파괴의 기전을 연구하고자 하였다. 경북대 병원 치주과 내원 환자중 2형 당뇨병 환자와 비당뇨 환자를 대상으로 여러 가지 환자요소, 임상 치주 상태를 기록하고, 비당뇨 환자의 만성 치주염 부위(n=8, group 1), 비당뇨 환자의 건강한 부위(n=8, group 2), 2형 당뇨병 환자의 만성 치주염 부위(n=8, group 3)에서 각각 변연 치은을 채득하고, 액화 질소에 급속 동결하였다. Western blotting을 이용하여 각 조직 내 MMP-2, MMP-8의 발현을 관찰, densitometer를 이용하여, 상대적 발현을 정량, 각 조직의 ${\beta}-actin$을 이용하여 표준화하여여 실험군들과 대조군들의 평균치를 비교하였다. 그 결과 정상군에 비해 치주 환자군에서 채득된 시편에서 MMP-2, MMP-8의 발현이 증가하였으며, 염증군의 당뇨군과 비당뇨군 사이에서는 2형 당뇨병을 동반한 치주 환자군에서 가장 높게 나타났지만, 통계학적으로 유의한 차이를 나타내지는 않았다(P<0.05). 결론적으로 본 연구에서 2형 당뇨병을 동반한 치주 환자군의 치은 조직에서 정상군과 비당뇨 치주 환자군보다 MMP-2, MMP-8의 발현이 다소 증가하는 양상을 보였다. The purpose of this study was to quantify and compare the level of MMP-2, MMP-8 in the healthy, inflammed gingival tissue and inflammed gingival tissue associated with type 2 DM. We investigate whether expression of MMP-2, MMP-8 is increased by chronic periodontitis associated with type 2 DM. Gingival tissue samples were obtained during periodontal surgery or tooth extraction. Based on patient's systemic condition & clinical criteria of gingiva, each gingival samples were divided into three groups. Group l(n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from 8 systemically healthy patients. Group 2(n=8) is inflammed gingiva from patients with chronic periodontitis. Group 3(n=8) is inflammed gingiva from type 2 diabetic patients with chronic periodontitis. Tissue samples were prepared and analyzed by Western blotting. The quantification of MMP-2, MMP-8 was performed using a densitometer and statistically analyzed by ANOVA. MMP-2, MMP-8 was expressed in all samples including healthy gingiva and increased in group 3 compared to group 1 and 2, and showed that significant variation was observed between group 1 & 3 in MMP-8 results. In conclusion, this study demonstrated that human gingival tissue with chronic periodontitis associated to type 2 diabetes showed slightly elevated MMP-2, MMP-8 levels compared to healthy gingiva and non-diabetic inflamed gingiva.

미세수술 술기 습득을 위한 태반의 사용

강민구,윤상엽,장학,Kang, Min-Gu,Yoon, Sang-Yup,Chang, Hak 대한미세수술학회 2007 Archives of reconstructive microsurgery Vol.16 No.2

Purpose: Microsurgical exercise has been performed with rat femoral vessels. But, it needs animal laboratory, anesthesia and its keeping facilities. Materials and Methods: Ten human placentas were studied for suitability in a microvascular exercise. The size and useful length of vessels were measured, and various microvascular anastomoses were performed. Result: The human placenta has many vessels traversing its fetal surface. A variety of sizes are available down to vessels of diameter 1 mm and up to vessels of diameter 6.5 mm (1.0<artery<5.3 mm, 2.4<vein<6.5 mm). Selection of suitable vessels can provide a vessel that can be satisfactorily used for microsurgical practice. The size of the placenta vessels compared well with reported sizes of digital vessels. Conclusion: We suggest the use of human placenta as complemetary to experimental animals in microvascular training. Where other preparations are not available, the human placenta appears to offer a reasonable, cheep and readily available alternative.