야생콩(Glycine soja Sieb. & Zucc.) 유전자원의 단백질, 지방 및 지방산조성 변이

김광철,박의호,Kim Kwang Chul,Park Eui-Ho 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1

우리나라 재래 야생콩 70계통들의 종실 성분들을 분석하여 활용 가능한 야생콩 계통을 선발함으로써 나물콩 품종 육성의 재료로 활용할 수 있는 기초 자료를 얻고자 수행된 결과를 요약하면 다음과 같다. 1. 조단백질 함량 범위는 $35.6-47.9\%$, 평균은 $42.3\%$였으며, 13계통은 $45\%$ 이상인 고단백 계통이었다. 조지방 함량은 $2.8-18.0\%$, 평균 $10.2\%$로 $3\%$이하인 2계통을 찾을 수 있었다. 2. 지방산 조성에서 16:0은 $11.1-44.6\%$, 18:0은 $3.0-11.4\%$, 18:1은 $11.1-30.6\%$, 18:2는 $12.4-59.5\%$, 18:3은 $1.1-17.3\%$ 범위였는데 특히 18:3 조성이율이 극히 낮은 2계통을 발굴하였다. 3. 조단백질은 조지방 및 지방산과 상관이 인정되지 않았고, 조지방은 16:0, 18:0함량과 고도의 부의 상관이, 18:2와 고도의 정의 상관을 그리고 16:0과 18:0, 18:1 간에는 고도의 정의 상관이 인정되었고, 18:2와 18:3과는 고도의 부의 상관이 인정되었다. This study was carried out to obtain useful information and to select promising materials for the breeding of sprout-soybean by the analysis of seed characteristics and chemical composition of Korean local wild soybean lines. For this purpose, crude protein, crude oil contents and fatty acid composition of 70 lines analysed. The crude protein content of Korean wild soybean lines ranged from 35.6 to $47.9\%$, mean was $42.34\%$, and 13 lines showed high protein content over $45\%$. The crude oil content of those ranged from 2.8 to $18.0\%$, mean was $10.2\%$, and 2 low lines were below $2\%$. The fatty acid 16:0, 18:0, 18:1, 18:2, 18:3 ranged $11.1-44.6\%,\;3.0-11.4\%,\;11.1-30.6\%,\;12.4-59.5\%,\;and\;1.1-17.3\%$ respectively, and 2 extremely low fatty acid(18:3) lines were selected. Significant correlation between crude oil and fatty acids(16:0, 18:0, 18:2), and among every fatty acid compositions were recognized. Promising wild soybean lines were selected as genetic resources for sprout-soybean breeding and research genotypes; YWS 104 and YWS189 as the high protein, YWS 28 and YWS30 as the low-crude oil and low 18:3.

Lipoxygenase 결여 콩 계통의 나물 특성 및 Lipoxygenase 활성

이형일,김광철,박의호,Lee Heung Il,Kim Kwang Chul,Park Eui-Ho 한국작물학회 2005 한국작물학회지 Vol.50 No.suppl1

본 연구는 lipoxygenase 결여 품종인 진품콩2호와 lipoxygenase 가 존재하는 광안콩, 소백나물콩, 푸른콩의 교배조합 내의 각 계통의 lipoxygenase 유무를 확인하고 activity 차이를 규명함으로써 lipoxygenase 결여 나물콩 육성의 가능성을 알아보고자 수행되었다. 콩나물 재배일수별 lipoxygenase 여부, 재배일수별 lipoxygenase 활성도 등을 분석한 결과를 요약하면 다음과 같다. 1. 콩나물 발아율은 대비품종과 계통들간의 큰 차이는 없었지만 정상 발아 개체율 에서는 계통이 $63\%$로 낮았으며 $T_{50}$은 1일로 소백나물콩, 푸른콩(0.6일)보다는 느렸으나 진품콩2호, 광안콩보다는 빨랐다. 2. 콩나물 생육은 치상 후 2일부터 콩나물 전체길이, 배축길이, 배축 직경을 조사한 결과 진품콩2호와 푸른콩 교배 조합 계통의 4537계통(콩나물 전체길이 13.9cm, 배축 길이: 7.3cm, 배축 직경: 2.2mm)이 대비품종들보다 높은 신장성을 보였다. 3. 콩나물의 물성은 치상 후 96시간 후에 hardness, cutting force, masticcation을 측정한 결과 lipoxygenase 결여 계통이 대비품종들보다 높게 측정되었다. 4. 콩나물 재배 일수 별 lipoxygenase band는 자엽부에서는 종실과 같이 lipoxygenase band를 확인 할 수 없었으며 배축부에서 모든 계통과 대비품종에서 확인 할 수 있었다. 5. 콩나물 재배 일수별 lipoxygenase 활성도는 2일차에 최고로 높았으며, 3일차에 최저치로 낮아진 후 다시 증가하는 양상을 나타내었다. 6. 각 교배 조합 내에서는 진품콩2호보다 활성도가 낮은 4510, 4522, 4537계통들이 lipoxygenase 결여 콩나물로서의 육종 가능성이 높을 것으로 생각되었다. This study was conducted to see the feasibility of breeding for sprout soybean cultivar with minimum benny flavor using lipoxygenase-less lines. Lipoxygenase-less cultivar Jinpumkong2 was crossed by lipoxygenase containing Gwangankong, Sebaeknamulkong, and Pureunkong as paternal parent and 24 lipoxygenase-less lines derived from those 3 combinations were selected and those lines were evaluated with their parental cultivars. Germination rate showed no difference between lipoxygenase-less lines and their parental cultivars, however, rates of normal sprout of those and Jinpumkong2 were 63 and $56\%$, and were lower than that of paternal parents. Hypocotyl length of those was same as Jinpumkong2, however, shorter than paternal parents. Texture characteristics including hardness, cutting force and mastication of 96 hour-cultured sprout of lipoxygenase-less lines showed higher value than that of their parental cultivars. Lipoxtgenase isozyme was not detected in the sprout cotyledon of lipoxygenase-less lines, however it was observed in the sprout hypocotyl of all the used genotypes. Though lipoxygenase activity in the seed of lipoxygenase-less lines was lower than that of Jinpumkong2(0.477, ${Delta}A$ 234 nm min-1 mg meal-1),2 lines revealed more than 0.5 value. Lipoxygenase-activity of 2 day-cultured sprout(both cotyledon and hypocotyl) was the highest, decreased in 3 days after culture and re-increased thereafter. Several lipoxygenase-less lines with lower lipoxygenase activity of sprout than Jinpumkong2 were selected and this suggested the possibility of breeding lines for soy-sprout with low benny flavor.

다량 뿌리혹 형성 콩 계통의 도입 개발 및 생육특성구명 1. 돌연변이유기에 의한 콩 초다뿌리혹형성 계통선발

이홍석,채영암,박의호,김용욱,윤광일,이석하,Lee, Hong-Suk,Chae, Young-Am,Park, Eui-Ho,Kim, Yong-Wook,Yun, Kwang-Il,Lee, Suk-Ha 한국작물학회 1997 한국작물학회지 Vol.42 No.2

Development of soybean cultivars with great nodulation and high nitrogen fixation activity, derived mostly from mutagenesis, may decrease inputs of chemical fertilizer nitrogen into the soil-plant system. Soybean seeds (cv. Jangyupkong, Hwanggeumkong, and Geomjungkong 1) were treated with three different levels of EMS (ethyl methanesulfonate) concentration(30, 50, and 70mM). Increasing the doses of EMS resulted in decreased field emergence rate of seeds, whereas it did not increase M$_2$ mutation frequencies. This indicated that the most efficient concentration of EMS was 30mM for generating mutants. Extensive mutagenesis of Sinpaldalkong 2 with 30mM EMS was undertaken to isolate soybean mutants with greater nodulation. Approximately 8, 200 M$_2$ families were screened for greater nodulation on 5 mM nitrate after inoculation with Bradyrhizobium japonicum strain YCK213-KFCC-10728. Mutant SS-2 nodulated more than the wild type. Comparison of supernodulation between SS-2 and two nts mutants(nts 1007 and nts 1116) revealed that SS-2 showed the supernodulation character at an earlier growth stage than the two nts mutants. Further studies should be needed to characterize the difference in timing of nodulation between SS-2 and nts mutants.

연구보문 : 식물환경 ; 다양한 빛 파장 및 식물 스트레스 유발 화합물이 콩나물 경도 및 유리아미노산 (Free Amino Acids)에 미치는 영향

차미정 ( Mi Jeong Cha ),박의호 ( Eui Ho Park ),강선철 ( Sun Chul Kang ),백광현 ( Kwang Hyun Baek ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4

BACKGROUND: Effect of various light wavelength and plant defense molecules were evaluated on the hardness and the contents of free amino acid including γ-aminobutyric -acid (GABA) in soybean sprouts. METHODS AND RESULTS: Germinating soybean seeds were treated with various single wavelength of light (380, 440, 470, and 660 nm) or without light at 25℃ for six days. Soybean seeds were also treated with stress-signaling molecule ethephon or H2O2 at the same time. Soybean sprouts treated with 470 nm substantially raised the hardness almost two times than the control. The free amino acid contents were higher in 470 nm and H2O2 treated soybean seeds than the control. Nutritionally beneficial GABA contents were increased by the treatments of 470 nm, 440 nm, ethephon, and H2O2. CONCLUSION: These results suggest that the hardness and the contents of amino acids can be regulated by stimuli, which stimuli could be composed of various wavelength and plant defense molecules. Especially, single wavelength 470 nm illumination has the effect of increasing GABA contents with increased hardness.

韓國 蒐集 在來種 조의 特性

Hong Suk Lee(李弘䄷,),Eui Ho Park(朴義浩) 한국육종학회 1987 한국육종학회지 Vol.19 No.2

To obtain basic information on the general characteristics of Korean local collections of foxtail millet, 88 local lines were collected widely throughout the country and investigated some morphological characteristics and studied also some ecological characteristics on the days from planting to heading and maturity by means of planting five times with 20 days interval. Ninty six per cent of collected lines showed green basal colour of culm and remains were purple or greenish purple. Most of collected lines showed cylindrical ear type and some of them were Indian club type, and only 2 lines showed branched type. Some morphological characters showed considerable variation ranging 103 to 162cm in culm length, 10 to 14 in the ratio of length to width of flag leaf, 6 to 10 in the ratio of length to thickness of millet ears, and 52 to 323g in the grain yield per 0.6m², respectively. But there were no close relation of collected lines with regional distribution in the above morphological characters. The days from planting to heading ranged from 72 days to 106 days and most of lines collected from southern part showed middle and late in heading. About 80% of collected lines showed 5 to 7 days in the shortening ratio of the days from planting to heading, and 87% of the lines ranged 7 to 10 days in the shortening ratio of the days from planting to maturity. The variation of shortening ratio of the days from planting to heading and muturity was not large enough to classify the ecotype of collected lines.

광픽업용 서스펜션의 개발연구

김윤영(Yoon Young Kim),윤민수(Min Soo Yoon),김진홍(Jin Hong Kim),박의호(Eui Ho Park),한준용(June Yong Han) 한국소음진동공학회 1996 한국소음진동공학회 학술대회논문집 Vol.1996 No.5

A. tuberosum, A. senescens 및 A. senescens var. minor의 생식양상에 관한 세포학적 고찰

김병주(Kim Byung Ju),권일찬(Kwon Yl Chan),한원영(Han Won Young),곽용호(Kwack Yong Ho),박의호(Park Eui Ho) 한국원예학회 1998 원예과학기술지 Vol.16 No.1

大豆의 世代短縮에 關한 硏究 : Effect of Different Day Length and Temperature Treatment on the Flowering and Seed Maturation of Soybean Cultivars -日長 및 溫度處理가 大豆品種의 開化 結實에 미치는 影響-

安容泰,黃鐘珍,李弘錫,朴義浩 서울大學校 農科大學 1980 서울대농학연구지 Vol.5 No.1

For the acceleration of generation advancement of soybean, the effects of four different day length and two different temperature treatments before and after flowering on the growth, flowering and seed maturation of soybeans were studied with six cultivars. The results of this experiment are summarized as follows: 1. The days from seeding to flowering was shortened by 2 days in Seocheonbaikdu and 18 days in Baikcheon by the short day treatment before flowering, while it was delayed by one or two days with long day treatment. 2. By the short day treatment before flowering the days from flowering to seed maturation was shortened by 7 days in Seocheonbaikdu and 10 days in Baikcheon, while little effect of long day treatment was obesrved. On the other hand, the effect of day length treatment after flowering on the seed maturation indicated significant varietal difference, and thus in Baikcheon seed maturation was shortened by 18, 7 and 7 days with short day treatment and delayed by 7, 7 and 10 days with long day treatment compared with control, according to the pre-flowering treatment respectively. But seed maturation of Seocheonbaikdu was affected little by day length treatment. 3. In the effect of day length treatment on the growth duration, interaction between cultivars and day length treatment before and after flowering was significant. The growth duration of Seocheonbaikdu were 69 and 79 days under short day and long day treatment before flowering and 78 days under control regardless of day length treatment after flowering, respectively. On the other hand, growth duration of Baikcheon were 77 days in short day treatment and 123 days in case of long day treatment before and after flowering, and showed 85 to 120 days according to the combination of day length treatment before and after flowering. But in general, day length treatment after flowering was more effective than treatment before flowering. 4. Days from flowering to maturity was reduced by 5 to 13 days in the medium and late maturing cultivars by short day and neutral day length treatment after flowering, but no effect in early cultivars was observed. 5. The days from flowering to maximum dry weight of seeds were 35 to 50 days under shortday or neutral day length treatment after flowering and 40 to 60 days in case of long day treatment compared with 35 to 60 days of natural day length. 6. Low temperature treatment after flowering delayed seed maturation significantly, especially in medium and late cultivars. 7. Both short day and long day treatments beore flowering decreased the number of flowers per plant, especially in Baikcheon. 8. The pod setting percent was affected greatly by day length treatment before flowering and indicated the highest percent of pod setting in case of short day treatment. The pod setting percent of Seocheonbaikdu ranged 42 to 60% and that of Baikcheon ranged from 24 to 65% depending on day length treatments before flowering. 9. The short day treatment before flowering decreased stem height, seeds per plant and 100 grain weight significantly. But long day treatment did not affect on the above characters. 10. Short day treatment after flowering increased pod setting percent and decreased 100 grain weight. In contrast, long day treatment decreased pod setting percent and increased 100 grain weight.

식품첨가제 처리가 콩나물 부패방지와 생육에 미치는 영향

박의호,최연식 한국콩연구회 1996 韓國콩硏究會誌 Vol.13 No.1

Soybean sprouts have been used as one of the major vegetables and prepared for diverse side dishes in Korea, During the cultivation of bean-spouts pathogens often induce seedling rot and detract the quality of bean-sprout products. This study was conducted to reduce the decay of bean-sprouts by food additive treatment. The effect of four food aditives - propionic acid (PA), acetic acid (AA), calcium propionate (CP) and sodium propionate (SP) - was tested. The seedling growth was inhibited severely by the treatment of 2% SP and 1% PA, AA and CP solutions, 0.7% CP and SP, and 0.4% AA and PA solution retarded the root growth of bean-sprouts and mild-damaged symptom appeared on the roots. When the sprouts were immersed 1 to 3 times in 0.5% CP and SP, 0.1% PA and AA solutions respectively during cultivation process, the growth of bean-sprouts and sprout yield were not affected, however, the color of seedling root was changed with 3 times treatment. The decayed ratio of bean-sprouts inoculated with Pseudomonas spp. was decreased significantly by the food additives treatment and bean-sprout yield showed increasing tendency compared with control, whereas the root growth of bean-sprouts was retarded.

열대지역의 두과작물 (荳科作物)

박의호 한국콩연구회 1988 韓國콩硏究會誌 Vol.5 No.2

Leguminesae is the third largest family of flowering plants and shows most promise for producing protein and oil at present and in the future of the thousands of known legume plants. Less than 20 crops have been used extensively including soybeans, peanuts, pigeon peas, chick peas, kidney beans, lentils, cowpeas, peas, mung beans and etc. Though the cultivation of legumes is the best way to produce food protein in developing country, many of the remaining leguminous crops are almost unknown to science. This paper provides a brief introduction to the leguminous crops cultivated in the tropics and subtropics to recognize its potentials and for future researches.