한국인 심부전증 환자 심근에서의 인형 거대 세포 바이러스 감염

이명용,이무용,김영권,한성식,최성준,김효수,이영우,서정돈 단국대학교 1998 論文集 Vol.33 No.-

Objectives: In order to evaluated the prevalence and the site of infection of cytomegalovirus in terminally failing heart, cytomegaloviral DNA was detected in the explanted hearts of transplantation recipients. Methods: DNA extractions were performed from explanted failing hearts(N=22) and normal hearts(N=5) and polymerase cain reactions(PCRs) were done for detection of late gene sequence coding pp150 glycoprotein. The products were confirmed by electrophoresis on the 1% agarose gel. n order to improve the detectability of cytomegaloviral genome, nested PCRs were executed with the primers designed for the original 607 bp products. In situ PCRs also were done with the samples which were confirmed as positive for CMV viral genome by nested PCRs. Results: All patients had IgG anti-cytomegalovirus antibody and did not have IgM anti-cytomegalovirus antibody. Cytomegaloviral genomes in myocardium were detected by polymerase chain reaction. The 607bp products by PCRs were found in both explanted failing heart(3 cases/22. 13.5%) and normal hearts( 1 case/5, 20.0%). In nested PCRs, 186bp products were found in both failing hearts(LV 4/22, LA 3/2, RV 4/22, RA 0/17) and normal hearts(LV 2/5, LA 1/4, RV 2/5, RA 2/5). These was no significant change in positivity of cytomegaloviral DNA genome between failing and normal hearts. Total positivity of cytomegaloviral genome in explanted hearts was 44.4% according to the nested PCR results. The positivity of cytomegaloviral DNA by n situ PCR was 33.3%(4/12), and the site of positive reaction was the nuclei of the myocardial cells. Conclusion: Cytomegalovirus was rarely observed in explanted hearts of terminal heart failure and nested PCR could enhance the sensitivity of cytomegaloviral genome detection. The result of the in situ PCR showed the site of the cytomegaloviral infection was nuclei of the myocardial cells. Cytomegalovirus, however, might have no direct causal relationship in the development of terminal heart failure.

흰쥐 시상하부에서 NMDA에 의한 c-fos의 발현

이영기,최기열,이원돈,유영복,이왕재,황덕호,조사선,장가용 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

Multiple myeloma 환자에서 Bortezomib치료 후 발생한 마비성 장폐색증 1례

김준영,이원식,손창학,박성길,진한영,박석주,조영완,정은욱,강명주,박정하,주영돈 인제대학교 2006 仁濟醫學 Vol.27 No.-

Bortezomib is the first clinically available proteasome inhibitor that is clinically effective in the treatment of multiple myeloma. A proteasome inhibitor acts through multiple mechanisms to arrest tumor growth, tumor spread, and angiogenesis. The main adverse effects of bortezomib are gastrointestinal symptoms, cytopenia, fatigue, and peripheral neuropathy. To date, severe paralytic ileus has not been reported as a toxic effect of bortezomib treatment in multiple myeloma. Bortezomib is a novel agent that has only been used clinically for 30 months, so a need exists to further evaluate its toxicity. We report a case of grade Ⅲ (NCI CTCAE v3.0) or grade Ⅳ(SWOG toxicity criteria) paralytic ileus in a 65-year-old man with relapsed multiple myeloma who underwent one cycle of single-agent bortezomib treatment scheduled in a 21-day cycle (1.3 mg/㎥ as a single i.v. bolus on days 1, 4, 8, and 11).

융모성림프구를 보이는 비장변연부 림프종 1예

진한영,손창학,주영돈,박정하,이재익,조영완,이원식 인제대학교 2006 仁濟醫學 Vol.27 No.-

Splenic marginal zone lymphoma is very rare B-cell lymphoma, characterized by an indolent clinical course. Clinical features are moderate-to severe splenomegaly, absolute lymphocytosis, and bone marrow intrasinusoidal infiltration of lymphocyte. We report the case of a 37-year-old male with SMZL. He complained of LUQ pain, who had a massive splenomegaly and moderate lymphocytosis in peripheral blood. Immunophenotyping findings and morphologic findings were consistent with SMZL. He underwent splenectomy and received chemotherapy with cyclophosphamide for eight months. Now, one year later after surgery he has a nearly normal blood count with no treatment.

Trigger point를 利用한 口眼괘斜 患者 10例에 대한 臨床的 考察

鄭榮敦,李浚龜,金永一,朴泰均,辛泳一,黃載然,李賢,李秉烈 대전대학교 한의학연구소 2002 한의학연구소 논문집 Vol.10 No.2

From July 1th 2001 to October 31th 2001, the Clinical investigation studies were carried out 10 cases of patient with Bell's palsy using Trigger point treatment group, who had been treated in Han-seung Oriental Medical Clinic. The results were like below : 1. The distribution of sex : Male were 4cases, female were 6case and the female to male ratio was 1.5 : 1. 2. The distribution of contributing factor : Unknown reason was most predominant 4 cases, and then were revealed to labor too hard, menttal stress(3 cases), trauma·common cold(1 case) in turn. 3. The distribution of perid to fall ill : Under a week was the most number 6cases, and then were revealed a week under two weeks(3 cases), over two weeks(1 case) in turn. 4. About period of treatment : The Trigger point treatment group is shorter than the Acupuncture treatment group. 5. The distribution of grade on admission in descending order : GrⅠ was the most number and then GrⅡ, GrⅢ in turn. 6. The distribution of clinical symptoms on adimission : Mastoid pain was the most number 9 cases and then lacrimal gland disorder 8 cases, dysgeusia 4 cases, hyperacusis 2 cases, the others 2 cases in turn. 7. About effect of treatment : The Trigger point treatment group is more excellent than the Acupuncture treatment group. 8. The more patient age is young, the more effect of treatment was excellent, had nothing to do with both groups. 9. The more perid to fall ill is short, the more effect of treatment was excellent, had nothing to do with both groups. 10. About period of improved frontal belly : The Trigger point treatment group is more excellent than the Acupuncture treatment group.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

특발성 과호산구 증후군 환자의 치료 도중 발생한 간혈종 1례

이영태,김동기,박지훈,김윤정,김기향,박보민,권민정,김애란,이원식,주영돈,손창학 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Idiopathic hypereosinophilic syndrome is characterized by marked elevation of eosinophil count ( >1,500/㎣) in peripheral blood without explaining etiology. It shows tissue involvement in many organs such as liver, G-I tract, myocar dium, lung, skin, kidney and nerve. Some case of hypereosinophilic syndrome with hepatic involvement have been reported, but not much. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 40 year old man who presented with RUQ pain. By abdominal CT, ill- defined subcapsular hematoma was detected. Liver biopsy showed eosinophilic infiltration within the portal tract and sinusoid. Drain procedure and corticosteroid adminiiatbrought marked improvement of clinical manifestation, hematologic abnormality and radiologic abnormality.

충청북도에서 분리한 느타리버섯 갈변병 병원세균의 병원성

이혁인,이승돈,박경선,김영기,차재순 충북대학교 농업과학기술연구소 1997 農業科學硏究 Vol.14 No.-

One hundred sixty seven bacterial isolates were obtained from the bacterial brown blotch-disea-sed oyster mushrooms collected from 37 mushroom cultivation houses throughout Chungcheungbukdo. The 21(11.9 %) of them formed white line when they were cultured with white line reacting organism(WLRO) and 14 (6.5%) were classified WLRO by white line test. All white line forming isolates against WLRO skewed strong pathogenicity and hemolytic activity to rat red blood cells. Six of the white line forming isolates were identified as Pseudomonas tolaasii which is known a pa-thogen of brown blotch disease of button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus) and four of them were Psudomonas fluorescens type G (biovar V) by BiologR bacterial identification system. WLROs consisted of heterogeneous group of bacteria ; Seven of them were identified as P. fluorescens type A (blovar I) and the rest of them were P. fluorescens type G,P. corrugata, P. tolaasii, and unidentified isolate. Some of the WLROs showed weak pathoge-nicity on rapid pitting test. P. tolaasii A- R and 6041R were mutagenized with transposon No. 5 (Tn5) and the mutants which could not form white line against WLRO were selected. All white line negative Tn5 mutants lost the pathogenicity and ability of hemolysis. The results suggest that tolaasin which is a lipodepsipeptide toxin produced by P. tolaasii and known an causal agent of white line forming in the bacterium is the primary disease determinant of P. tolaasii.

용접공들의 면역능에 관한 연구

이수일,조봉수,김영욱,고광욱,조원근,김영실,강수용,황인경,조병만,김돈균 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The authors examined human immunity indicators such as CD4, CD8, Natural Killer(NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studies on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615±650 ea/㎕ compared with 2,368±681 ea/㎕ in control group(p>0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37.5±9.4%, 24.0±8.8%, and 21.0±9.5%, respectively, and 35.5±6.5%, 25.0±6.9%, and 19.6±8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. but there was no statistical significance between two groups(p≥0.05). 3. IgG, IgA, and IgM in welder group were 15.9㎎/㎗, 2.7㎎/㎗ and 1.5㎎/㎗, respectively, and 15.6㎎/㎗, 2.9㎎/㎗ and 1.7㎎/㎗ in control group. There was no statistical significance between two groups(p≥0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2±1.8㎍/㎗, 0.4±0.38㎍/㎗, 1.2±0.6㎍/㎗ in welder group and 3.2±1.7㎍/㎗, 0.44±0.29㎍/㎗, 1.1±0.6㎍/㎗ in control group, respectively, and these value were within normal ranges.

자성선숙어(protogynous fish)의 성전환

이정재,김형배,박인석,고환봉,이영돈 濟州大學校海洋硏究所 1993 해양과환경연구소 연구논문집 Vol.17 No.-

제주 연근해에 서식하는 자성선숙형 자웅동체어인 놀래기류와 능성이류를 대상으로 성전환에 따른 생식소의 조직학적 변화와 체색의 변화 등의 성적이형 현상을 토대로 하여 성전환 특성을 고찰하였다. 1. 용치놀래기, 어렝놀래기, 놀래기는 6월과 7월에 주산란을 하는 하계산란종인 반면에 황놀래기는 10월과 11월에 산란하는 추계산란종이였다. 2. 성전환 체장범위는 용치놀리기 13. 0-15. 0㎝, 황놀래기는 11.5-16.0㎝, 그리고 놀래기 전장 10.0-13.0㎝였다. 3. 성전환은 주로 산란기 전후로 하여 나타나고 있다. 용치놀래기, 어렝놀래기, 놀래기는 1차웅과 2차웅이 혼재하고 잇으나 어렝놀래기는 2차웅만이 출현하였다. 4. 성전환에 따른 용치놀래기, 어렝놀래기, 황놀래기 체색변화는 선홍색 또는 선황색 계통에서 색조가 다양한 녹색이나 황색계통으로 전환되고 있다. 놀래기의 경우 전반적의 체색의 변화는 미미하나 몸 가운데에 파란반점과 가로줄무늬를 띠고 있다. 어렝놀래기 경우 체색의 변화와 함께 등지느러미 제1, 2극의 돌기가 신장되고 있다. 5. 성전환 과정에 따른 놀래기류의 생식소내 조직학적 변화는 난모세포의 퇴행변성에 따라 다양한 간질세포와 혈관의 발달을 보였다. 이후 난소의 박판주위에서 정원세포와 정모세포들이 출현하기 시작하여 기능적 정소로 전환되고 있다. 6. 자바리는 전장 30.0-92.3㎝, 능성어는 전장 23.4-38.7㎝에서 생식소내 주변인기 난모세포를 갖는 암컷이었고, 붉바리는 전장 22.0-33.0㎝에서 양성생식소를 갖는 개들도이 출현하고 있다. Studies and reviews have been based on sex reversal diagnosis of histological change and body color change by sexual dimorphism on protogynous hermaphrodite fish, Labridae, Serranidae inhabited at the costal area of Cheju-do, Korea. Halichoreres poealopterus, Pteragogus flagellifera and H, tenuispinis were summer season, spawing species which mainly spawn in Jun and July, but Pseudolabrus japonicus was autumn season spawing species which mainly spawn in October and November. The standard length range of sex reversal were 13. 0-15. 0㎝ H, poecuilopterus, 9. 5-15.0㎝ P,flagellifer, 11. 5-16 0㎝ P, japonicus, and 10. 0-13 0㎝ (total length) H, tenuispinis respectively. Sex reversal mainly appeared before and after spawing season. H. Poecilopterus, H. tenuispinis and P. japonicus were composed of monandry and diantry, but P. flagellifera was diandry. By sex reversal, the body colors of Ho poecilopterus, P. flagellifera and P. japonicus change from pale red or yellow lines to brilliant green or dark yellow lines. The body colors of H. tenuispinis change slight, but containe lateral stripe pattern and blue spot in body center. In P.flagellifera, 1st, 2nd spine process of dosal fin were grown with body colour change. In sex reversal process, histological change of Labridae of inner gonad showed development of various interstitial cells and blood vessel by degeneration of oocyte. Thereafter, spermatogonia and spermatocyte begin to appear at the inner edge regions of the ovarian lamellae and then converted from ovary to functional testis. Epinephelus moara of 30. 0-92.3㎝ in TL, E.Septenfasciatus of 23. 4-38.7㎝ in TL were female with contained oocyte of perinucleolus stage. E. akaara of 22.0-33.0㎝ in TL appeared individuals of ovotestis.