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The Change of Immunoactivity of Dendritic Cells Induced by Mouse 4-1BBL Recombinant Adenovirus
Kuang Youlin,Weng Xiaodong,Chen Zhiyuan,Zhu Hengcheng,Chen Hui,Jiang Botao,Liu Xiuheng 연세대학교의과대학 2010 Yonsei medical journal Vol.51 No.4
Purpose: The purpose of this study is to construct a recombinant adenovirus vector carrying mouse 4-1BBL and observe its effects in dendritic cells. Materials and Methods: Mouse 4-1BBL cDNA was taken from the plasmid pcDNA3-m4-1BBL and subcloned into adenovirus shuttle plasmid pAdTrack-CMV, and then transformed into competent BJ5183 with plasmid pAdEasy-1. After recombination in E. coli, Ad-4-1BBL was packaged and amplified in HEK 293 cells. The expression of 4-1BBL in Ad-4-1BBL-transfected mouse prostate cancer cell line RM-1was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. After the co-culture of dendritic cells (DCs) with Ad-4-1BBLtransfected RM-1 cells, interleukin (IL)-6 and IL-12 production were assessed by enzyme-linked immunosorbent assay (ELISA) and co-stimulatary moleculs (CD80 and CD86) on DCs were analyzed by flow cytometry. Results: The levels of IL-6 (3,960 pg/mL) and IL-12 (249 pg/mL) production in Ad-m4-1BBL-pulsed DCs were more than those in none-pulsed DCs. The differences were statistically significant (p < 0.05). The expression of co-stimulatary molecules (CD80 and CD86) was up-regulated in Ad-m4-1BBL-pulsed DCs. Conclusion: The results indicated the recombinant mouse 4-1BBL can effectively activate DCs.
Yongjun Tan,Li Zhou,Kaiqi Gu,Caihong Xie,Yuhan Wang,Lijun Cha,Youlin Wu,Jiani Wang,Xiaosong Song,Xia Chen,Hua Hu,Qin Yang 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.4
To conduct the association between vitamin B12 and mental health in children and adolescents. Five databases were searched for observational studies in any language reporting on mental health and vitamin B12 levels or intake in children and adolescents from inception to March 18, 2022. Two authors independently extracted data and assessed study quality. Qualitative and quantitative analysis of data were performed. The review was registered in the PROSPERO database (CRD42022345476). Fifty six studies containing 37,932 participants were identified in the review. Vitamin B12 levels were lower in participants with autism spectrum disorders (ASD) (standardized mean difference [SMD], −1.61; 95% confidence interval [95% CI], −2.44 to −0.79; p < 0.001), attention deficit hyperactivity disorders (SMD, −0.39; 95% CI, −0.78 to −0.00; p = 0.049) compared with control group. Vitamin B12 intake were lower in participants with ASDs (SMD, −0.86; 95% CI, −1.48 to −0.24; p = 0.006) compared with control group, but showed no difference between depression group (SMD, −0.06; 95% CI, −0.15 to 0.03; p = 0.17) and the control group. Higher vitamin B12 intake were associated with lower risk of depression (odds ratio [OR], 0.79; 95% CI, 0.63−0.98; p = 0.034) and behavioral problems (OR, 0.83; 95% CI, 0.69−0.99; p = 0.04). The vast majority of included studies supported potential positive influence of vitamin B12 on mental health, and vitamin B12 deficiency may be a reversible cause for some mental health disorders in children and adolescents.
Banpu Ruan,Zhenyu Gao,Juan Zhao,Bin Zhang,Anpeng Zhang,Kai Hong,Shenglong Yang,Hongzhen Jiang,Chaolei Liu,Guang Chen,Youlin Peng,Guojun Dong,Longbiao Guo,Zhengjin Xu,Qian Qian 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4
Leaf color-related mutants are important materialsto study the chlorophyll biosynthesis and chloroplastdevelopment. Here, we isolated and characterized a thermosensitiveyellow-green leaf mutant, ygl, through mutagenizedJaponica rice cultivar Zhonghua11 by EMS. The mutantshowed yellow-green leaves only at the seedling stage. Genetic and molecular analyses uncovered that YGL encodean Mg2+-chelatase ChlD subunit, which was a new allele ofChl1/YGL7. Quantitative PCR and GUS staining showedthat the YGL transcripts expressed in all tested organs andsubcellular localization detected YGL protein in the chloroplasts. The mutant displayed yellow-green leaves at 20°C andnormal green leaves at 34°C during the seedling stage. Transcript level of YGL in the ygl mutant was significantlyreduced at 20°C, and increased at 34°C compared with thewild-type plant. Furthermore, the expression of chlorophyllbiosynthesis and chloroplast development related genes wereinfluenced by the mutation of YGL at different temperature. These findings suggested that the YGL gene not only play akey role in chlorophyll biosynthesis, but is affected attranscript level by temperature in rice.