Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

액체 ^⁴He에서의 μ^- 및 π^- X-선 강도 계산

김승곤 全北大學校 敎育大學院 1984 敎育論叢 Vol.4 No.-

Cascade calculations have been performed in an effort to better fit measured absolute yields of muonic and pionic X-rays in liquid ^4He. With the bell-shaped intial distribution calculated by Haff and Tombrello a slight improvement over the last calculation was made in agreement with the measured mesic X-ray yields.

Staphylococcus aureus동정에 있어서 Staphyslide-Test(Slide agglutinotion)의 고찰

김성권,석종성,김승곤 최신의학사 1984 最新醫學 Vol.27 No.9

The coagulase test in used to differentiate species within the genus staphylococcus. A positive coagulase test (tube or slide) is usually final diagnostic criterion for the identification of staphylococcus. But this test has many advantages such as the preparation of plasma, the appearance of false positivity by other bacteria and long incubation of negative coagulation. Staphyslide test (Slide agglutination) is known to use the principle that a protein receptor capable of combing with fibrinogen in on staphylococcus aureus. Staphyslide- test is more sensitive and specific than other test for the identification of staphylococcus aureus. 1. Among the total of 104 strain of staphylococcus, illustrations shown positivity in the tests of staphyslide test and coagulase test were 76 strain (73.0%) and 72 strain (69.2%) in each cases. Among the 76 strain those illustrated positive result in staphyslide-test, the four 4 strain illustrated dormant results in the normal coagulase test and coincided point ratio was 94.7%. 2. Among the strain those have illustrated analysis of mannitol in mannitol salt agar, strain shown positive vesulte in the test of staphyslide test and normal coagulase test were 76 strain (93.8%) and 72 strain (88.9%) in each cases. 3. In comparison with 100% of veappearance, repeated test of staphyslide test, the coagulase tube test showed occasional dormancy and reapprearnace was 87%. 4. In staphyslide test, showed good sensibility, peculiarity and reappearance in comparison with the normal coagulase test in metivation of S. aureaus, so it is believed that it has a strong point to obtain prompt results.

태권도 뒤차기 동작의 운동학 및 동작 유사성에 관한 연구

김승곤,오선홍 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

To attempt understanding of similarity of Diechagi movement in TaeKwonDo's movement with Cheju-do's representive athletes(N=5), the present study undertaken to investigate several factor influencing to similarity & accuracy when repeating it. The analysis method adapted the angle-angle diagram encoded chain in function of time. The conclusions obtained were as follows: 1. The difference of the elapsed time during Diechagi movement according to signal or non-signal didn't show significance statistically and increasing regression trend of mean cumulated elapsed time Y=O.l2X + 0.06, R^2=0.7973 in case of signal and Y=O.O83X + 0.155, R^2=0.743 in case of non-signal. 2. The first , vertical angular displacement of trunk during response- impact showed rather high increasing trend in signal than non-signal. Y=14.75X + 80.25. R^2=0.9648 in signal and Y=l7.504X + 66.814. R^2= 0.9696 in non-signal. and second, vertical & horizontal displacement of COG by each phase didn't show statistically significant difference by signal or non-signal.. each showed mean 9.64^+_4.10cm in vertical and mean 32.01±5.60cm in horizontal displacement in signal, and showed mean 10.35±3.51cm in vertical. mean 43.06±8.14cm in horizontal displacement in kinematic variables. 3. The similarity coefficient by each trial of chagi movement didn't show irregular pattern and as a characteristics. proximal endpoint in degree of similarity showed higher than distal endpoint of segments, and showed total mean 56.25% similarity this study's subjects in each 53.22% of KTH. 60.75% of SEC. 78.02% of KHJ, 43.19% of JMJ

Pre-Stress 狀態에서 一定한 反復變位에 依한 砂岩의 疲勞破壤擧動에 關한 硏究<第2報> : 力學的係數들과 彈性度 및 塑性度의 變化 Variations Mechanical Moduli, Elastic and Plastic Degree

金溢中,金昇坤,金永錫 全北大學校 1989 論文集 Vol.31 No.-

The uniaxial cyclic displacement tests were performed to investigate and examine the fatigue failure behavior of sandstone at the staged pre-stress levels and under constant-displacement boundary conditions. The experimental results reported here have been limited to variations of elastic moduli, elastic degree and plastic degree with the staged pre-stress levels and the number of cycles. Some of the conclusions which may be derived from the results of the studies are as follows : 1. When the rocks failed, the elastic moduli and the elastic degree rapidly decreased, but the plastic degree rapidly increased. The higher the rocks were loaded in comparison with previous ones, the higher the plastic degree, and the shorter fatigue life. 2. In a second run after recess the displacement, chord modulus was also rapidly increased at the second cycle, because a large quantity of plastic strain was produced at the first cycle. 3. The fatigue life must be relatively determined not only with respect to the decreasing rate of compression modulus and dynamic compression modulus, but also the plastic degree and plastic strains. 4. The elastic degree was not proportional to the elastic strains, while the plastic degree was proportional to the plastic strains 5. As an increasing rate of strain decreases, recovery modulus and chord modulus curves approach each other, and their separation means that fatigue limits have been reached.

노랑초파리에 대한 Esterase Isozyme의 변이에 관한 연구

김승곤 최신의학사 1984 最新醫學 Vol.27 No.9

1983년 7월부터 10월사이에 노랑초파리(D. melanogaster)를 3개 자연집단 부천, 전주, 울산에서 채집한 것을 사용하여 한천 Gel 전기영동법으로 Esterase Isozyme을 분석하였다. Est-C는 3개(C_(O), C^(S), C^(F))의 복대립유전자로 분리되었고, Est-6도 3개(6^(O), 6^(S), 6^(F))의 복대립유전자를 포함하고 있었다. Est-C lows의 빈도는 지역적으로 차이는 있으나 어느 지역이던지 Est-CF가 0.9616이었고 Est-6^(S)가 0.9396로 가장 높게 분석되었다. Est-C와 Est-6의 연쇄형은 전주가 95% 울산이 90% 이상씩 연쇄되어 있었다. Esterase isozyme was analysed by the method of agar-gel electrophoresis in the flies collected from three localities, Bucheon, Cheonju and Ulsan. Est-C was devided in 3 multiallele (C^(O), C^(S) and C^(F)), and the patterns of Est-6 was also isolated 3 allele (6^(O), 6^(S) and 6^(F)). The frequency of Est-C locus was some different in 3localities, and the high frequencies of Est-C^(F) (0.96) and Est-6^(S) (0.94) was detected compared to the other locus.

감마선 및 엑스선의 피이크 분석

김승곤,허영회,박광준 대한방사선방어학회 1984 방사선방어학회지 Vol.9 No.1

核分裂 및 放射化生成物質에서 放出하는 여러가지 감마선들은 核燃料를 破壞하지 않고도 推出할 수 있는 많은 情報를 包含한다. 그러나 半導體 檢出器에서 얻은 복잡한 스펙트럼에서 이러한 정보를 推出하기가 容易하지 않기 때문에 電算코드의 使用이 必要하게 된다. 本 硏究에서는 그동안 國際的으로 널리 普及되어 使用되는 감마선 分析 프로그램들의 長點을 취하여, 감마선은 물론 X-線의 스펙트럼도 피팅(fitting)하여 피이크의 중심과 면적을 正確히 計算할 수 있는 전산코드 CAERI를 開發하였다. CAERI는 FORTRAN으로 쓰여있고, 특히 固有輻射線幅(natural line width)을 무시할 수 없는 X-線의 피이크 表現函數인 Voigt 函數에 대해서는 다른 X-線 分析 프로그램들이 使用한 간단한 近似式 대신에, 더욱 正確한 無限級數近似式을 사용하였다. 특히 CAERI는 U이나 Pu과 같은 重元素의 核種分析時에 直面하는 감마선과 U이나 Pu의 X-線이 任意로 ()()하여 共存하는 복잡한 스펙트럼까지도 取扱할 수 있다. ()Lu감마선과 ()U K()X-線의 試驗스펙트럼을 피팅하여 다른 프로그램들의 피팅결과와 비교했을 때 좋은 一致를 보았다. A great variety of nuclear gamma rays emitted from fission and activation products of spent nuclear fuel contains much information that can be clicited without affecting the integrity of the fuel elements. But the extraction of such information form the comples spectrum is difficult and requires computer codes. In the present work, a versatile code "CAERI" was developed which located peaks and calculates their areas for X-rays as well as gamma rays using elegant features of some widely used program for gamma-ray peak fitting. "CAERI"coded in FORTRAN used infinite series approximation more accurate than other workers various simple, piecewise series approsimations for evaluations of the Viogt function which represents the X-ray peak with non-negligible natural line width. "CAERI" can handle even a complex multiplet consisting of peaks from X-rays and gamma rays in arbitray mixture, which one often encounters in the isotopic analysis of heavy elements such as U and Pu. The results of the fitting performed on the test spectra of ()Lu ()-ray and ()U K() X-ray show good agreement with those by previous workers.

겔화 반응 및 열처리한 금속염화물의 결정 및 구조특성

김인태,박환서,유승곤,김환영,김준형 한국공업화학회 2003 응용화학 Vol.7 No.2

A new concept for the stabilization and solidification of volatile radioactive wastes via gelation in a simple material system is introduced in this paper. Using sodium silicate as a reaction module and phosphoric acid as a catalyst or stabilization agent. CsCl is chemically converted to thermally stable compounds. The heat-treated product at 1000℃ contained a cristobalite crystalline framework and a glassy phosphorus-rich phase with a high Cs content. The morphology looked like honey flowing on the honeycomb. From TG/DT analysis, Cs could not be vaporized below 1300℃ by changing the aging conditions and molar ratio of Si/p. As a new concept, GRSS(gel-route stabilization and solidification) could give us the chance to make the high temperature waste conditioning process more stable and economical.

中世 國語의 假定形語尾「??」과 抑揚形語尾「??녀」考

金昇坤 건국대학교 1975 論文集 Vol.2 No.1

파이온 비임 요법의 물리에 관한 연구

金承坤,姜正求,李延玉 全北大學校 1994 論文集 Vol.38 No.-

Monte Carlo calculations were performed for the transport of the photon, electron, and pion in the water phantom in an effort to quantify the effects of the three ionizing radiations for cancer therapy. Comparison of energy deposited by the three particles as a function of penetration shows that in the case of the negative pion, the normal Bragg peak is greatly enhanced by the energy deposited due to nuclear capture of the pion by a nucleus and the subsequent nuclear reaction. The results clearly indicate that a preferred method of radiation therapy is to use energetic negative pions which has a number of advantages over the other two particles.