면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

구개주름의 형태형성과 Retinoic Acid로 유발된 이상

김혜진,신웅기,권오유,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

Morphogenesis of palatal rugae and retinoic acid-induced anomalies, numerical and pattern disruptions, were studied to know the mechanism of normal rugae formation and the relations between these disruptions and retinoic acid teratogenecity. Sprague-Dawley rats and all trans retinoic acid of Sigma Chemicals were used in this experiment. Retinoic acid was administered at gestational day 8, 9, 10, 11, and 12 by the dose of 60mg/kg 80mg/kg 100mg/kg and 120mg/kg respectively per orally. At the gestational day 17.5, the rats were sac rificed under ether anesthesia and removed entire uterine horns into the saline-filled Petri dish. Congenital anomalies on the head and palatal rugae were observed with stereoscope. and light and transmission electron microscopic observation were performed to investigation histologic changes of the rugal epithelium and mesenchyme after retinoic acid treatment. The results were as follows; 1. Retinoic acid-induced congenital anomalies in this experiment were exencephaly and hydrocephalus in gestational day 8 and 9 groups, and cleft palate in gestational day 9 and 10 groups, and the number of anomalies was increased dose dependently. 2. Severe numerical variation and pattern disruptions such as division, medial and lateral fusion, cross, bifid rugae and rugal papillae were found on the gestational day 9 and 10 groups, and the relation between the retinoic acid teratogenecity and the numerical and pattern disruptions of the rugae were very close. 3. On TEM observation, the basal laminae under the rugal epithelium were absent at many areas and the filopodiae of the epithelial cells protruded into the underlying mesenchyme. According to the above results, it is considered that the mesenchymal cells of palatal shelf migrate into the designated areas of epithelium through the basal lamina, proliferate rapidly and form the rugae. Retinoic acid may inhibit the proliferation and migration into the epithelium of mesenchymal cells and the incompatibility between the growth of palatal shelf and the slowed speed of mesenchymal cells migration into the epithelium will leads the numerical and pattern disruptions of the rugae. And the relation between the teratogenecity of the retinoic acid and the numerical and pattern disruptions of the rugae was considered to be very close.

건국 우유와 유제품의 소비성향에 관한 조사연구

임종우,김종우,조광호,김영주,유제현,윤여창,장주익,권일경,안효일,이용규,이부응,박종래,이헌종 경상대학교 축산진흥연구소 1989 畜産振興硏究所報 Vol.16 No.1

신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

韓國人의 各 臟器組織中 靑酸鹽의 微量分布에 關한 硏究(Ⅱ)

張成吉,金恩浩,金英雲,李鎭勳,柳在薰,權五星,金明德,吳世珍,金由那 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

말초혈액림프구, HaCat세포 및 MRC-5세포에서 Aphidicolin에 의해 유도되는 Fragile sites

박경란,구동억,이증훈,김원식,이영호,권오유,장성익 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.5

갑상선 세포에서 Peroxiredoxin에 의한 과산화수소 및 아포프토시스 조절

김현진,이태훈,김도희,권오유,김영건,송민호,노흥규,박수정,김호,박은신,정효균,서재미,채수홍 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.1

Background : Peroxiredoxins(Prx) play an important role in regulating cellular differentiation and proliferation in several types of mammalian cells. One mechanism for this action involves modulation of hydrogen peroxide(H2O2)-mediated cellular responses. This report examines the expression of Prx I and Prx II in thyroid cells and their roles in eliminating H2O2 produced in response to TSH. Methods : The expression of Prx-I and Prx-II were quantiated in FRTL-5 after stimulation with Thyroid stimulating hormone (TSH), Forskolin (FSK), Methimazole (MMI) and hydrogen peroxide (H2O2). Transient transfections were carried out with FRTL-5 cells at 80% confluency and 20?g of pCRprx I and pCRprx II or equivalent molar amounts of the pCR3.1TM basic vector. Transient transfection used an electroporation technique. Intracellular H2O2 was assayed in FRTL-5 cells with a fluorescent dye, 2', 7'-dichlorofluoresceindiacetate(DCFH-DA). Apoptosis of cells were evaluated by using an detection kit (Promega, Inc., Madison, WI). Results : Prx I and Prx II are constitutively expressed in FRTL-5 thyroid cells. Prx I expression, but not Prx II expression, is stimulated by exposure to TSH and H2O2. In addition, methimazole(MMI) induces a high level of Prx I mRNA and protein in these cells. Overexpression of Prx I and Prx II enhance the elimination of H2O2 produced by TSH in FRTL-5 cells. Treatment with 500?M H2O2 causes apoptosis in FRTL-5 cells as evidenced by standard assays of apoptosis(i.e., terminal deoxynucleotidyl transferase deoxyuridine triphosphate-biotin nick end-labeling(TUNEL), BAX expression and PARP cleavage. Overexpression of Prx I and Prx II reduces the amount of H2O2-induced apoptosis measured by these assays. Conclusion : These results suggest that Prx I and Prx II are involved in the removal of H2O2 in thyroid cells, and can protect these cells from undergoing apoptosis. These proteins are likely to be involved in the normal physiological response to TSH-induced production of H2O2 in thyroid cells(J Kor Soc Endocrinol15:55-69, 2000).

Molecular chaperone as a sophisticated intracellular membership

Kwon, O-Yu,Shong, Min-ho 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Molecular chaperone의 발견은 생명과학자들에게 살아있는 세포 내에서 어떻게 생체활성단백질이 만들어지고 유지되는지에 대한 자극과 함께 그것을 증명하기 위한 실험동기를 부여하였다. 초기에는 molecular chaperone이 nucleosomes의 assembly에 관하여는 단백질을 설명하기 위하여 사용되었으나, 지금은 기본적인 세포생리기능의 하나인 단백질의 folding과 assembly를 돕는 assistant protein으로 주로 사용된다. 단백질합성 뿐만 아니라 단백질수송, oligomeric structure의 assembly와 disassembly, heat shock을 포함한 각종 내, 외부스트래스에 의해서 변성된 단백질의 세포내분해와 회복에도 molecular chaperone이 관여하고 있다. 그러나 아직까지는 molecular chaperone들의 3차구조와 그들간의 상호작용에 관한 정보가 부족하여 크게 진전되지 못하고 있지만, 많은 연구자에 의한 정보축적으로 인하여 빠른 시일 내에 molecular chaperone의 세포내역할이 분명하게밝혀질것이다. Discovery of molecular chaperones has stimulate cell biologists and thus made it possible to re-examine the processes whereby proteins achieve and maintain their functional conformations within living cells. The term 'molecular chaperone' was first coined to describe one particular protein involved in the assembly of nucleosomes, but the term has now been extended to describe the function of a wide variety of proteins that assist protein assembly in a wide range of fundamental cellular processes. Besides protein synthesis, these processes include protein transport across membranes, folding of nascent polypeptide, the assembly and disassembly of oligomeric structures, and the recovery or removal of proteins damaged by various environmental stresses including heat shock. Progress of molecular chaperones research is still limited by the lack of 3-dimensional structural information and detailed interacts with target proteins in the cell. However, several laboratories around the world are attempting to extend our knowledge on the functions of molecular chaperone, and such efforts seem justified to finally provide the answers to the most burning questions shortly.

The Production of Heterologous Proteins Using the Baculovirus Expression Vector System in Insect Cells

Kwon, O-Yu,Goo, Tae-Won,Kwon, Tae-Young,Lee, Sung-Han Korean Society of Life Science 2002 Journal of Life Science Vol.12 No.2

The baculovirus expression vector system (BEVS) is one of the powerful heterologous protein expression systems using insect cells. As a result this has become a hot issue in the fleld of biotechnology. The advantage of the BEVS is that the large-scale production of heterologous proteins, which undergo posttranslational modification in the endoplasmic reticulum (ER), can be accomplished. Altrough posttranslational modification of heterologous proteins in insect cells is more similar to mammalian cells than yeast, it is not always identical. Therefore, aggregation and degradation can sometimes occur in the ER. To produce a high level of bioactive heterologous proteins using BEVS in insect cells, the prerequisite is to completely understand the posttranslational conditions that determine how newly synthesized polypeptides are folded and assembling with ER chaperones in the ER lumen. Here, we provide information on current BEVS problems and the possibility of successful heterologous protein production from mammalian cells.

A Misfolded Thyroglobulin is Retained by Molecular Chaperones in the Endoplasmic Reticulum

Kwon, O-Yu,Seong, Yeon-Mun,Shong, Min-Bo Korean Society of Life Science 1999 Journal of Life Science Vol.9 No.1

Deficient thyroglobulin is one of the important causes of congenital hypothyroid goiter with a prevalence of -1/40,000 humans. We now demonstrate that in cog/cog mice showing hypothyroidism, four endoplasmic reticulum-molecular chaperones stably bind to thyroglobulin, providing insight into physiologic regulation of endoplasmic reticulum storage diseases.