KI-180과 KI-188 칼슘조성물이 어린쥐의 성장발육에 미치는 영향

박승만(Seung-Man Park),성기승(Ki-Seung Seong),이종석(Jong-Seok Lee),이옥환(Ok-Hwan Lee),한찬규(Chan-Kyu Han) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

본 연구에서는 KI-188 칼슘과 KI-180 조성물이 성장발육에 미치는 효과를 조사하였다. 시험기간중 KI-188 칼슘과 KI-180 식이군이 대조군 보다 성장률과 평균 식이섭취량은 증가하였다. 체장은 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 평균 3.55 mm 더 길었고, 등뼈길이는 KI-180 식이군이 대조군 보다 통계적인 차이는 없었지만 0.5 mm 더 길었다. 대퇴골 무게는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 무거웠으며, 대퇴골길이는 대조군에 비해 각각 평균 0.89, 1.09 mm 더 길었다. 혈당, 총콜레스테롤 및 중성지방 농도는 차이가 없었고, 칼슘농도는 KI-188 칼슘과 KI-180 식이군 보다 대조군이 유의하게 높았다. 백혈구와 혈소판수는 차이가 없었고, 적혈구, 혈색소 및 헤마토크릿치는 대조군이 KI-188 칼슘과 KI-180 식이군 보다 통계적으로 높았다. ALP활성은 KI-180 식이군이 대조군 보다 통계적으로 높았고, osteocalcin농도는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 유의하게 높았다. 혈청 testosterone 농도는 KI-188 칼슘과 KI-180 식이군과 대조군 간에 차이가 없었다. IGF-1과 IGFBP-3 농도는 KI-180 조성물이 대조군 보다 각각 20%, 11% 정도 유의하게 더 높았다. 이상의 결과를 검토할 때 KI-188 칼슘과 KI-180 조성물은 어린쥐의 성장과 골격의 발달을 촉진시키고, 성장호르몬의 분비능을 양적으로 유도하여 전반적인 성장발육에 유효한 작용이 있는 것으로 사료된다. Herbs have active components that promote the growth rate of both animals and human. The KI-180 and KI-188 calcium food formulae contain Acanthopanacis cortex, Bombysis corpus and hoelen, seaweed calcium, chlorella extract, spirulina, colostrum powder, and other natural and functional components. We evaluated the growth-promoting effects of these formulae by analyzing the weight, femur and backbone, alkaline phosphatase, osteocalcin, testosterone, insulin-like growth factor (IGF)-1, and IGF binding protein-3 (IGFBP-3) of growing rats. Growing rats administered with KI-180 and KI-188 calcium showed the increase of body weight, body length, and femur weight and length of growing rats. In addition, administration of KI-180 and KI-188 calcium increased the alkaline phosphatase activity, the levels of osteocalcin and the growth hormones IGF-1 and IGFBP-3 of growing rats. The impact of KI-180 and KI-188 calcium on the physical development of growing rats suggests that the incorporation of these food formulae in the diets of growing children may promote the physical development.

한국인 폐확산능 정상예측식의 임상적 유용성과 정확성

나승원 ( Seung Won Ra ),박태선 ( Tai Sun Park ),홍윤기 ( Yoon Ki Hong ),홍상범 ( Sang Bum Hong ),심태선 ( Tae Sun Shim ),임채만 ( Chae Man Lim ),이상도 ( Sang Do Lee ),고윤석 ( Youn Suck Koh ),김우성 ( Woo Sung Kim ),김동순 ( Dong 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.2

연구배경: 폐확산능을 해석하는 데 필요한 정상예측식으로는 한국인을 대상으로 하여 박 등이 개발한 식(박 식)이 있으나 아직 외국 정상예측식을 많이 사용하고 있다. 이에 국내에서 많이 사용하는 외국 정상예측식인 Burrows 식과 박 식의 임상적 유용성과 정확성을 비교하고자 하였다. 방법: 1. 임상적 유용성 연구; 2006년 7월부터 12월까지 6개월간 폐확산능검사를 시행한 환자 중 두 식을 각각 정상예측식으로 적용하였을 때 폐확산능 해석이 다른 276명(대상군 A)을 대상으로 하였다. 대상군 A에게 두 식을 각각 적용하였을 때 폐확산능 해석과 임상적 판단과의 일치도를 비교하여 임상적 유용성을 평가하였다. 2. 간질성폐질환 진단의 정확성 비교; 2001년부터 2006년까지 폐조직검사를 시행하여 확진된 간질성폐질환군과 서울아산병원에서 모집한 정상군을 대상으로 하여 정상예측식으로 두 식을 각각 적용하였을 때 폐확산능 해석의 정확도를 비교하였고, 두 식이 차이가 나는지 맥니머의 카이스퀘어 검정을 하였다. 결과: 1. 임상적 판단과의 일치도 비교; 276명을 임상정보를 토대로 폐확산능을 예측하여 분류한 결과 정상 54명, 감소 220명, 불분명이 2명이었다. 예측식으로 박 식과 Burrows 식을 적용하였을 때 임상적 판단과 일치하는 환자는 각각 78%와 22%이었다(p<0.001). 2. 간질성폐질환 진단의 정확성 비교; 박 식은 민감도 90.1%, 특이도 100%이었고 Burrows 식은 민감도 64.2%, 특이도 100%로 민감도가 통계학적으로 유의하게 박 식이 높았다(p<0.001). 결론: 우리나라 정상예측식인 박 식이 외국 정상예측식인 Burrows 식을 정상예측식으로 적용하는 것보다 임상적 유용성이나 간질성폐질환 진단의 민감도에서 더 우월하였다. 향후 폐확산능검사의 정상예측식으로 박 식을 사용해야 할 것으로 사료된다. Background: Park et al. developed the Korean reference equation for the measurement of diffusing capacity in 1985. However, the equation has not been widely used in Korea and foreign reference equations have been popularly used. We intended to compare the clinical usefulness and the accuracy of the the Korean reference equation (Park`s equation) with that of the foreign equation (Burrows` equation) that is commonly used in Korea. Methods: 1. Evaluation of clinical usefulness; Among 1,584 patients who underwent diffusing capacity (DLCO) at the Asan Medical Center from July to December 2006, group A subjects included 276 patients who had different interpretations of DLCO in trials employing Burrows` equation and Park`s equation. Clinical assessment was decided by consensus of two respiratory physicians. In order to evaluate the clinical usefulness of Burrows` equation and Park`s equation, agreement of clinical assessment and DLCO interpretation were measured. 2. Evaluation of accuracy; Group B subjects were 81 patients with interstitial lung disease (ILD) and 39 normal subjects. The 81 ILD patients were diagnosed following a surgical lung biopsy. The accuracy of diagnosing ILD as well as sensitivity and specificity were evaluated according to the use of the reference equations (Burrows` equation and Park`s equation) for DLCO. Results: Agreement between clinical assessment and interpretation of DLCO was 22% for the use of Burrows` equation and 78% for the use of Park`s equation. The sensitivity and specificity of the Burrows` equation for diagnosing ILD were 64.2% and 100%. The sensitivity and specificity of the Park`s equation for diagnosing ILD were 90.1% and 100%. The sensitivity of the Park`s equation for diagnosing ILD was significantly higher than that of Burrows` equation (p<0.001). Conclusion: The Korean reference equation (Park`s equation) was more clinically useful and had higher sensitivity for diagnosing ILD than the foreign reference equation (Burrows` equation). (Tuberc Respir Dis 2008;64:80-86)

도심에 거주하는 남성 노인에 있어서 유산소 운동능력이 혈관내 염증수치와 동맥경직도에 미치는 영향

안기만(Ki man Ahn),김윤신(Yoon shin Kim),박원하(Won Hah Park) 한양대학교 고령사회연구원 2012 한양고령사회논집 Vol.3 No.1

Experimental epidemiology and clinical studies have provided strong evidence that physical exercise has beneficial effects on multiple physiological variables affecting cardiovascular health. Cardiopulmonary function has been previously associated with atherosclerotic disease and coronary events. The aim of this study was to examine the higher levels of cardiopulmonary function are associated with lower levels of hs-CRP(high sensitive C reactive protein) and baPWV(barcahial ankle pulse wave velocity) in Korean elderly men. Study participants were 115 elderly men (mean age 62.5±2.2) participated in the research. Age, body mass index(BMI), resting heart rate, blood pressure, medical history, lipid profile, high sensitivity C-reactive protein(hs-CRP), Brachial-ankle pulse wave velocity (ba-PWV), cardiopulmonary function were measured. VO2peak higher elderly man had a significantly decreased hsCRP(0.22±0.26, p<0.05) and PWV (1439.1±159.9, p<0.05). In addition, HDL-C(55.2±14.2, p<0.05) levels was significantly increased and LDL-C(1193±21.7, p<0.05) level was decreased. This study show that VO2peak levels are positively correlated with hsCRP, HDL-C and PWV. Apparently healthy subjects with increased inflammatory markers have increased systemic arterial stiffness, which might reflect early atherosclerotic changes. Higher physical conditioning status, indexed by VO2peak, was associated with reduced arterial stiffness and hsCRP and increased HDL-C. The results of our study suggest that high cardiopulmonary function reduces hsCRP levels and improves PWV, HDL-C in Korea elderly men. 심혈관질환은 현재 선진 산업 국가들에서 성인 사망 제 1원인으로 알려져 있다. 심혈관질환에 의한 사망률은 나이가 증가하면서 더 높아지는 것으로 보고되고 있다. 이러한 심혈관질환을 일으키는 위험인자로는 나이, 신체활동의 부족, C반응성 단백(CRP), 혈관경직도(baPWV) 등이 심혈관질환을 높이는 것으로 보고되고 있다. 무엇보다 인구의 고령화로 인해 심혈관질환이 증가하고 있다는 것이다. 심혈관질환을 예방하기 위해서는 생활 습관 개선이 중요하며 그 중에서도 신체활동은 매우 중요하다. 하지만 고령의 건강한 남성을 대상으로 심폐기능과 관상동맥질환의 위험인자와의 관련성을 살펴본 연구는 부족하다. 따라서 본 연구는 60세 이상의 건강한 노인 남성(62.5±2.2)을 대상으로 심폐체력의 수준에 따른 혈중 C반응성 단백(CRP), 혈관경직도(baPWV)의 변화를 알아보고자 했다. 심장병 증상이 없는 노인 남성 115명을 대상으로 하였으며, 심폐기능 측정을 위해 운동부하검사를 실시했다. 혈액검사를 통해 혈중 CRP와 동맥경직도를 평가하기위해 baPWV 검사를 실시하였다. 그 결과 혈중 CRP는 심폐기능이 높은 그룹에서 0.22±0.26로 심폐기능이 낮은 그룹의 0.49±0.49 보다 유의하게 낮은 것으로 나타났다(p<0.05). baPWV역시 심폐기능이 높은 그룹에서 1439.1±159.9로 심폐기능이 낮은 그룹의 1523.7±236.1보다 낮게 나타났다(p<0.05). 따라서 건강한 노인 남성의 경우 심페기능이 좋은 그룹에서 심혈관질환 위험인자인 CRP와 baPWV가 유의하게 낮은 것으로 나타났다.

人口政策에 따른 諸課題

朴聖根(Park Seong Kun),Koh Kap Suk(토론자),Chang Man-Ki(토론자) 한국인구학회 1979 한국인구학 Vol.2 No.1

There are two main problems in formulating population policies. First, is it reasonable to formulate population policy in isolation from other social and economic policies? It is the personal belief of Mr. S. Park that a population policy should be regarded as a part of other social and economic policies. Second, there appears to be no clearly formulated long range objectives in conducting the current population redistribution policy; 1) Community development program should be based on the recognition of the special characteristics of the area. And the re-location program for industries should be strongly supported with special tax and loan consideration. 2) The policy aimed at the dispersion of population should be conducted with the program of providing an equal educational opportunity regardless of geographical location, and family planning. Discussants: Koh, Kap Suk and Chang, Man-Ki. 1. Urbanization may be considered as an inevitable result of industrialization. In order to regulate effectively the population movements into urban centers, there should be a clearly formulated population policy in co-ordination with other social and economic policies. 2. Let us assume that Korea will achieve the zero population growth rate by the year 2,000. The fact that currently there are no long range "visional" proposals to deal with the population composition and distributional problems indicates that there has been no comprehensive research about population planning. 3. The optimal population size for Korea should not be thought of in terms of a static concept but a dynamic concept which is related to manpower supply and housing problems. 4. The problem of aging should not be looked at solely as an economic issue. Instead, it should be examined from a humanistic point of view where high priority is placed on restoring human dignity for the older citizens. Response of Mr. Park: 1. There is a tendency to over-emphasize the economic side of population issues. It should be pointed out that a man not only provides labor which is an input for the production of economic goods, but also he is a consumer for whose welfare economic goods are produced in the first place. It should be remembered that economic growth has beneficial impacts on the population but it also has harmful effects. 2. In order to make the government officials aware of population issues, it has been proposed to the Population Policy Evaluation Committee that the courses in population studies be included in the study program for the re-education of government employees. 3. Issues involved in population dispersion: Urbanization is an inevitable process of economic development. Problems arising from urbanization should be solved not by forcefully preventing the migration of population from rural areas to urban centers but by reducing the rural-urban gap in the living conditions. This policy has already been implemented to some extent as indicated by the government's policy of decentralization of industries and Saemaul Movement. In summary, the basic policy of government is to slow down the rural-urban migration by providing incentives to stay in farms in the rural area rather than by the strong-handed method of preventing the internal migration.

단무지 공장에서 발생한 질식 사고의 원인과 방사선학적 소견: 중례보고 및 가스분석 결과

박충기,김만구,김흥철,안범규,박만수,황우철,최철순,강익원 江原大學校 附設 環境硏究所 1994 環境硏究 Vol.11 No.-

목 적:단무지 공장에서 질식사고를 일으켰던 가스의 종류를 알아내고, 이러한 가스중독의 방서선학적 소견을 소개하기 위하여 본 연구를 하였다. 대상 및 방법:단무지 공장에서 발생한 질식 사고자 3예중 생존자 1예를 대상으로 단순흥부X-선검사와 CT 소견을 분석하였으며, 가스의 종류를 알아내기 위하여 단무지를 유리병에 넣어 발생된 가스를 가스크로마토그래픽을 이용하여 분석하였다. 결 과:단무지 공장에서 발생한 질식사고자중 생존한 1명의 방사선학적 검사에서 신속히 호전되는 폐경결(consolidation)을 볼 수 있었으며 이는 폐부종의 소견임을 알 수 있었다. 협기성 상태에서 단무지를 담은 유리병의 상층부 가스를 실험 분석한 결과 이산화탄소, 에칠알코올이 다량으로 검출되었으나 독성가스는 주로 황화수소이였다. 결 론:단무지 공장의 질식 사고자에서 폐방사선학적 소견은 폐경결을 보이는 폐부증으로서 이는 실험을 통해 단무지에서 발생되는 유독가스인 황화수소 때문임을 입증하였다. Purpose: To identify the main toxic gas released from salted radish in rice bran(Dan-M-Ji) and to introduce the radiological findings of the patient who was exposed to the gas. Materials and Methods: Chest radiographs and CT scans of one survivor among three men who were exposed to the gas from Dan-Mu-Ji were reviewed. Gas obtained from the closed bottle containing Dan-Mu-Ji was analized by using the gas chromatography. Results: The radiographlc examinations of the survivor were suggestive of pulmonary edema with it's rapidly improving consolidations in both lung. The headapace gas within the bottle containing Dan-Mu-Ji was mainly composed with carbon dioxide, ethyl alcohol and hydrogen sulfide, of which hydrogen sulfide was considered the main toxic gas released. Conclusion: Under the anaerobic condition, Dan-Mu-Ji released toxic hydrogen sulfide. Inhalation of hydrogen sulfide might produce non-cardiogenic pulmonary edema.

籠球 運動과 撓骨 및 中手骨 成長과의 關係

박인기,백만수,박진권 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1

The effects of basketball training on the structural aspects of the radius and metacarpal have been analyzed. Research sample groups consist of a player group and a general student group. The player group consists of 48 students whose grade varies from the fifth grade of primary school to the second grade of boys' high school. They had been regularly trained 3 days (6 hours) per week. The general students group consists of 60 students who did not participate in the training. From these comparative study, we have found that noticeable growth of the redius proximal end and distal end of the player group had occurred from the time of the first grade of middle school which was the third year of training. We also found little difference in the growth of the redius shaft. Notable growth of the metacerpal proximal and distal end as in the radius has been abserved from the time of the first grade of middle school. Noticeable growth of the third metacarpal shaft had occurred from the time of the sixth grade of primary school which was the second year of training. The second, fourth, fifth metacarpal shaft had growed noticeably from the time of the first grade of high school which was the sixth year of training.

한국인 안검의 생체계측지

박동만,송중원,한기환,강진성 大韓成形外科學會 1990 Archives of Plastic Surgery Vol.17 No.5

Plastic surgeons who frequently deal with trauma and deformity of the eyelids and orbits, need have an abundant knowledge of the accurate anatomical morphology and acceptable proportion of them. The normal value of the anatomical morphology and function of the eyelid has not yet been standardized in Korea. The authors measured anthropometry of normal Korean eyelids and statistically analysed in 2,151 individuals including 1,078 male and 1,073 female. Statistical analysis of these measurements offers valuable information about the chronological growth, size, and shape of healthy Korean eyelids. The results are as follows : 1. There was no significant statistical difference between the right and left sides in all measurements. 2. The growth of the eyelid reached the adult level at about 20 years of age and then the growth can be divided into 3 patterns, the first one slowly increased, the seconded one was a stationary, and the last one slowly decreased. 3. The following statistics were recorded for males and females. The cornea-lateral canthal distance was 7.9mm and 7.3mm, the diameter of the cornea was 13.8mm and 13.6mm cornea-medial canthal distance was 7.8mm and 7.5mm the horizontal dimension of the palpebral fissure was 29.7mm and 28.4mm, the vertical dimension of the palperbral fissure was 7.9mm and 8.0mm, the slant of the palperbral fissure was 9.5 degrees an 10.6 degrees, the intercanthal distance was 36.5mm and 37.2mm, the width of the glabella was 24.4mm,. and 24.1mm, the interpupillary distance was 66.1mm and 63.9mm, the ectocanthion-superior margin of the palpebral fissure distance was 14.9mm and 14.2mm, the endocanthion-superior margin of the palpebral fissure distance was 14.9mm and 14.2mm, the height of the opened upper eyelid was 10.3mm and 10.0mm, the height of the closed upper eyelid was 18.2mm and 18.1mm, levator function by the Berke's method was 16.2mm and 15.6mm, MLD was 7.2mm and 7.1mm, and MRD1, was 2.7mm and 2.6mm respectively. 4. The vertical dimension of the palpebral fissure, slant of the palpebral fissure, and height of the double eyelid fold were greater in females than males. The intercanthal distance and width of the glabella was nearly the same in males and females and other measurements were greater in males than females. 5. A double eyelid fold was seen in about 36% of males and 50% of females. The average height of the double eyelid fold with the eye closed in 20 year old females was 5.2mm and 30 to 50 year old females was 5.5mm. The most frequent shape of the double eyelid fold was type 5. 6. The epicanthus was present in 58.1% in this series and existed in over 90% under age 10, but disappeared in about 90% of the people over 50. The most frequent shape of the epicanthus was tarsalis type.

만성폐쇄성 폐질환 환자에서 적혈구 항산화효소의 변화

국기용,이만재,조희충,임영국,박일,김원식,이승일,이병래 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Toxic oxygen free radicals have been implicated as important pathologic mediators in many clinical disordesrs. Enhandng the intracellular content of antioxidant enzymes can provide means of limiting biological damage caused by oxygen free radicals. Many investigations have examined the potential of various oxyradical scavengers to modulate oxyradical injury. The oxygen free radicals play a role in the pathogenesis of chronic obstructive pulmonary disease. To investigate the pulmonary oxyradical injury and the protective role of antioxidant enzymes, author measured the amount of thiobarbituric acid reactants, the activiteies of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and the sulfhydryl groups in serum and red blood cells from the chronic obstructive pulmonary disease groups and the normal controls. The thiobarbituric acid reactant in chronic obstructive pulmonary disease groups was increased than the controls in both serum and red blood cells, and the superoxide dismutase activity in red blood cells was no statistically difference in both groups. But, the glutathione peroxidase and catalase activities were lowered in chronic obstructive pulmonary disease groups than the controls. The sulfhydryl groups in serum and red blood cells were no statistically difference in both groups. These results suggest that the increased thiobarbituric acid reactant in chronic obstructive pulmonary disease groups mean oxygen radical toxicity, and the decreased glutathione peroxidase and catalase activities may play a role in cellular injury.

Helicobacter pylori 항원을 이용한 면역우유 생산 및 항체특성에 관한 연구

배만종,김수정,예은주,김병기,박창호,김미경 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 위염, 위궤양, 위림프종 및 위암과 같은 소화기 질환의 원인균으로 알려진 Helicobacter pylori균을 항원으로 하여 젖소에 면역시킨 후 생산된 우유의 anti-H. pylorigkdcp의 생성능을 검토하고, 백신투여량과 항체 생성과의 관계, 항원 항체의 특이성, H. Pylori균 응집력, 항체의 산과 열에 대한 안정성, 그리고 백신투여가 젖소에 미치는 영향을 알아보았다. 1. 백신 투여량에 따른 혈청과 유청내의 anti-H. pylori항체의 함량은 10㎖, 20㎖, 30㎖ 백신투여 모든 군에서 대조구에 비해서 높은 양의 항체 생성을 확인하였다. 유의적인 차이는 없었으나 20㎖투여가 항체 생성이 가장 많은 것으로 조사되었다. 백신 투여량에 따른 유청내의 anti-H. pylori항체 생성량은 혈청에서 나타난 결과와 유사한 양상으로 형성되었다. 2. Anti-H. pylori항체의 SDS-PAGE에 의한 분자량측정 결과 heavy chain은 50kDa정도, light chain은 24kDa정도로 확인 되었다. 3. H. pylori항원 단백질의 분자량측정 결과 12개의 band가 형성되었다. Anti-H. pylori의 항원 특이성을 알아보기 위해 western bloting을 한 결과 혈청, 혈청정제, 유청, 유청 정제 모두 7개의 항원성 물질을 확인할 수 있었고, 주 항 원성 물질은 분자량이 97, 66, 34kDa 이었다. 4. 응집반응결과 유청속의 anti-H. pylori항체가 H. pylori균에 대해 1/10의 응집가를 나타내었다. 5. Anti-H. pylori항체의 산·알칼리에 대한 안정성 실험에서는 pH 5 ∼ pH 10 범위에서 안정한 상태로 100%의 활성을 나타내었다. 6. Anti-H. pylori항체의 열에 대한 안정성 실험에서는 60℃에서 60분간 안정한 상태를 보였고, 70℃에서도 비교적 안정한 상태였으나 60분 경과후 40%정도 활성이 감소하였다. 80℃에서는 4분간 처리했을 때 77%의 활성을 유지하였고, 100℃에서도 1분간은 비교적 안정한 상태였다. 7. 백신투요로 인하여 유량이 12% 감소하는 경향을 나타냈고, 최장 1주일 정도 지나면서 회복되었다. 8. 백신투여 후 젖소의 체온을 측정한 결과 대조구에 비해 정상적인 범위 내에서 체온이 상승하는 경향을 나타내었다. This study has been to examine bio-function of anti-H. pylori antibodies of milk produced from cows immune with antigen germ of Helicobacter pylori and search the relation between vaccine dosage volume and antibody formation, peculiarity of antigen antibody, cohesion of H. pylori germ, stability about add and heat of antibody, and impact of vaccine dosage on cows. The content of serum and Anti-H. pylori antibody within whey in accordance with vaccine dosage volume has confirmed the formation of high-quantified antibody compared to the controlled conditions in all groups vaccine dosages of 10㎖, 20㎖, and 30㎖. It has been turned out that the antibody was farmed most in 20㎖ dosage on while there was no attention difference. The molecular weight of Anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50kDa in the heavy chain and about 24kDa in the light chain. 12 bands were formed as the result of measured molecular weight of antibody protein. The western blotting was performed in order to examine the antigen peculiarity of Anti-H. pylori that all 7 antigen substances including serum, serum refining, whey and whey refining could be confirmed and the main antigen substances were 97, 66, 34kDa of molecular weight. As a result of cohesive response Anti-H. pylori antibody in whey showed 1/10 cohesive rate about H. pylori germ. In stability test about acid and alkali of antibody there was 100% activated in the range of pH 5-pH 10. In stability test about heat it showed stable condition in 60℃: for 60 minutes and comparatively stable condition in 70℃, but reduced activation to 40% after 60 minutes. It maintained 77% activation in 80℃ for 4 minutes and comparatively stable in 100℃ for I minute. It was inclined to reduce to 12% of flow caused by vaccine injection, but recovered after about maximum 1 week. In measurement of body temperature of cows after vaccine injected, it was inclined to rise with the normal scope in comparison with the controlled conditions.

한국 복싱의 退潮에 관한 小考

김원기,전만중,박상호 한국체육사학회 2003 체육사학회지 Vol.12 No.-

This paper started from a necessity to reconsider boxing sports, the only sports game that brought about our patriotism during the time when most of us were poor, and flied away out stress and anger, also, that made us dream and have a hope. The boxing game attracted people's interest at that time, meeting the spirit of hungry. However, boxing sports became an unpopular game nobody pays attention to. The reason was that all boxing games mobilized many boxing players and enthusiastic audience in the past, but as GNP elevated greatly, there are few hungry persons, the spirit of hungry was subdued, the number of players drastically decreased, demand of audience reduced, people don't enjoy watching boxing games, there are few spectators in boxing gyms, no sponsors support relay broadcasting for boxing games. Furthermore, results have been very poor in various amateur international boxing match, all broadcasting stations became to dislike relay broadcasting of international matches. A certain broadcasting station regularly sent out pro-boxing game programs every weekend for 45 years as it took a full charge of pro-boxing game, but frequency of relay broadcasting reduced now, and the reason that the popularity rating is lower than other popular sports games made the station avoid relay broadcasting, These results mean that reducing number of TV relay broadcasting showing the highest PR effect made boxing games inactive. Summing up the study, the reasons that boxing games have declined popular sports games' appearing, high-leveled foreign players' watching TV games, the law of diminishing marginal utility, and democratized society. As a result, demand of boxing games rapidly reduced. However, boxing games in Korea must remember Kim, Ki-su's acquisition of championship on July the 25th, 1966, WBA championships obtained by 16 players including Hong, Su-wan, Yu, Myung-woo, Park, Jong-pal, Mun, Seong-kil, and 11 WBC championships acquired by Yeon, Dong-kyun, Jang, Jeong-gu. They surely won an international fame flying the taegeuk flag all over the world. Also, in spite of short history of woman pro-boxing, Lee, In-young acquired a world championship, therefore, Korea still has a hope to meet another new jumping moment.