팽이버섯 재배 현장에서 Listeria monocytogenes의 성장을 억제하기 위한 살균 처리 기술 개발

박경민,이수빈,정도영,최송이,황인준,김세리,Kyung Min Park,Su-Bin Lee,Do-Young Jung,Song-Yi Choi,Injun Hwang,Se-Ri Kim 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

본 연구는 팽이버섯 생산현장에서 사용하는 권지 접착포에서 팽이버섯으로 Listeria monocytogenes 교차오염을 예방하기 위하여 화학적 살균제에 의한 L. monocytogenes 저감 효과를 확인하였다. 과산화초산, 미산성전해수, 이산화염소수, 차아염소산나트륨을 10분간 처리했을 때 권지표면에 부착된 L. monocytogenes를 최대 99.99% 이상 저감화시켰고, 이들 4종의 살균소독제는 팽이버섯 유기물 존재 여부에 관계없이 높은 살균능을 보이므로 권지의 세척소독에 활용가능성이 있음을 확인하였다. 특히 과산화초산은 권지 접착포 거친면과 부드러운면 모두에서 L. monocytogenes를 99.999% 이상 감소시킬 수 있으므로 팽이버섯 권지 세척시 30mg/L 이상의 과산화초산에 30분 이상 침지한 후 사용하면 권지에 오염된 L. monocytogenes의 교차오염을 예방할 수 있을것으로 사료된다. The consumption of enoki mushrooms has been associated with cases of listeriosis produced by Listeria monocytogenes, highlighting the significance of sanitizing food-contact surface, such as the velcro used in welding processing of enoki mushrooms, to ensure microbial safety. We investigated the inhibitory activity of nine chemical disinfectants at regular concentrations against L. monocytogenes isolated from a mushroom farm environment. The bacterial suspension was prepared in phosphate buffered saline and mushroom extract broth and inoculated onto the velcro surface. After inoculation, most disinfectants reduced the initial 8 log CFU/coupon concentration by less than 2 log CFU/coupon during a 5-min treatment. Slightly acidic hypochlorous water showed a reduction of approximately 4 log CFU/coupon when tested for more than 30 min at the maximum allowable concentration of 200 mg/L. Sodium hypochlorite solution showed a reduction of approximately 5 log CFU/coupon when used at 100 mg/L for 60 min. Peracetic acid, at the maximum allowable concentration of 300 mg/L, showed the most effective reduction of 5 log CFU/coupon or more when the surface was treated with 37.5 mg/L for 30 min. These results indicate that peracetic acid can be used as the disinfectant strategy to control cross-contamination of L. monocytogenes on the velcro surface of plastic wrappers used in the welding processing of enoki mushrooms.

임신 입덧에 의해 발증한 베르니케뇌증 환자에서 스테로이드 다량요법과 FLAIR MR기법

한영민,유도성,김영주,허필우,조경석,김달수,강준기 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.12

CMAS公法에 있어서 固定 및 回轉媒體를 利用한 染色廢水의 處理에 關한 硏究

閔庚碩,金弘泰,都仲鎬 경북대학교 산업기술연구소 1990 産業技術硏究誌 Vol.18 No.-

The purpose of the research is determination of the substrate removal efficiencies and sludge productions in complex dyeing wastewater treatment using the Fixed/Submerged Moving Media Complete Mixing Activated sludge(CMAS) process. Laboratory scale CMAS reactors were operated with the organic loading rates from 0.38 to 1.07㎏BOD/㎥/day. The sludge productions were similar in both the fixed and submerged moving media systems. During operating periods the sludge was removed periodically in fixed media system, yet not removed in submerged moving media.

한국산 독말풀에 대한연구(Ⅰ) : HPLC에 의한 Hyoscyamine 과 Scopolamine의 정량

도경삼,신민교,송호준,선중기,손인경,백승화 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3

A new method for quantitative determination of Hyoscyamine and Scopolamine in Datura stramonium L. by High Performance Liquid Chromatography(HPLC) was established. A reversed-phase system with a μ Bondapak C18 column using methanol in 0.2% aqueous phosphoric acid(40:60) with PIC A as a mobile phase was developed. Hyoscyamine and Scopolamine were detected at 254nm and the analysis was successfully carried out within 20min.

Lime-Fly Ash/Rice Husk Ash에 의한 해성 퇴적토의 강도특성 개선

민덕기,이경준,김현도 울산대학교 2001 공학연구논문집 Vol.32 No.1

본 연구에서는 울산광역시 남구 삼산동 일원의 지표 하 7m 지점에서 채취한 시료에 대하여 생석회 처리 후 FA(fly ash)와 RHA(rice husk ash)를 혼합하여 강도특성을 분석하였다. 연구방법으로는 채취한 시료와 혼합토에 대하여 기본물성시험과 일축압축강도 시험 및 C.B.R 시험을 실시하였다. 그리고 첨가재의 혼합에 따른 입자구조를 분석하기 위하여 주사전자현미경(SEM)을 이용하여 비교 · 검토하였으며, 첨가재의 화학적 구성성분을 X-선 형광분석(XRF)을 통하여 조사하였다. 화학성분 분석결과, FA의 주 구성성분은 Al2O3, Fe2O3가 약 50%, RHA의 경우는 SiO2가 약 91%를 차지하는 것으로 나타났다. 일축압축강도 시험결과, FA와 RHA가 각각 10% 첨가시 가장 큰 강도를 나타내었으며, 10% 이상 첨가시 강도가 저하되는 경향을 나타내었다. FA와 RHA가 각각 10% 첨가된 시료에 대한 C.B.R 시험결과에서 전자의 경우 118%, 후자의 경우 100%를 나타내었으며, 소성지수(PI)가 각각 9.4%, 9.1%값을 나타내어 소성지수 저감 후 보조기층, 기층재로써 사용이 가능할것으로 판단되었다. In this paper, a laboratory investigation was carried out to estimate the strength improvement of quicklime mixture with fly ash and rice husk ash for the effective use of surplus soils, and the shear strength with curing time was estimated at lime 10 percent with the change of fly ash and rice husk ash content. The effect of strength improvement has been established through the change of fly ash and rice husk ash content from the samples taken at Samsan region, Ulsan. The change in the microstructure of materials was investigated by using the scanning electron microscope(SEM). The chemical composition of materials was investigated by using the X-ray fluorescence spectrometer(XRF). The result of XRF analysis indicate that Al2O3 and Fe2O3 contents of fly ash is about 50%, SiO2 content of RHA is about 91%. The results of another tests indicated that the presence of lime with fly ash and rice husk ash encouraged the stabilization efficiency of lime with fly ash and rice husk ash, and increased shear strength. Furthermore, it is necessary for inquiring into the relationship between the characteristics of strength and the chemical components. Key words : fly ash, rice husk ash, quick1ime, surplus soils, SEM, XRF

재생골재 콘크리트 강도의 조기추정에 관한 실험적 연구 : 마이크로파 가열기법을 중심으로 Focusing on Microwave Method

이민경,박희곤,양동일,김우재,이영도,정상진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

In many countries a considerable amount of demolition wastes is generated and wastes concrete constitutes a significant proportion of the construction waste. Therefore, the necessity for the use of recycled aggregate in concrete arise and the reuse of a waste concrete may solve the problems of environmental pollution and shortage of natural aggregate. According to this study is to examine the possibility of site adoption microwave-use early strength estimation method. Based on the existed study related the plain concrete, the interrelation between recycled aggregate concrete and microwave-use early strength estimation method is required.

일개 제빵회사 근로자의 제빵공 천식 유병률

김정민,권영준,주영수,임형준,이태경,강혜련,백도명 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.3

목적: 일개 제빵회사에서 제빵공 천식 유병률을 조사하여 제빵공 천식으로 인한 국내 질병부담을 파악하는데 기초자료를 제공하고자 하였다. 방법: 일개 제빵회사의 생산관련 근로자 596명에서 수습기간(3개월) 중이거나 참여를 거부한 216명을 제외하고,380명을 연구대상으로 하였다. 밀가루분진 노출형태에 따라 직접노출군과 간접노출군으로 직무 카테고리를 분류하고,설문조사는 ISAAC 설문항목에 직업력 등을 추가한 설문지를 사용하였다. 설문조사에서 천식이나 알레르기병과 관련된 증상이나 병력이 전혀 없는 간접노출군은 임상검사대상에서 제외하였다. 임상검사대상 233명에게 피부단자검사,혈청 특이 IgE 항체검사,산업의학 전문의와의 면담을 시행하였고,면담에서 천식이나 알레르기비염과 관련된 증상이나 병력이 확인된 31명을 연속적 PEFR 측정대상으로 하였다. PEFR 변동률로써 메타콜린 기관지유발검사대상을 선정하였고,메타콜린 기관 지유발검사에서 기관지과민성이 확인된 경우에 특이 기관지유발검사를 시행하였다. 제빵공 천식은 특이 기관지유 발검사에서 천식반응이 확인된 경우로 정의하였다. 결과: 연구대상의 밀가루 민감화율은 21.0%(직접노출군 25.9%,간접노출군 13.3%), 제빵공 천식 유병률은 3.2%(직접노출군 5.1%,간접노출군 1.8%)였다. 직접 노출군이 간접노출군에 비해 밀가루에 민감화될 위험이 높았으며(OR 2.15,95% CI=1.03∼4.51), 제빵공 천식에 이환될 위험은 통계적으로 유의하지 않았다(PR 2.52,95% CI=O.78∼8.18). 밀가루에 민감화된 군이 그렇지 않은 군에 비해 제빵공 천식에 이환될 위험이 매우 높았다(PR 38.63, 95% CI=6.14∼243.22). 결론: 이번 연구결과는 직업적으로 밀가루에 노출되는 국내 근로자에서 제빵공 천식이 적지 않을 것임을 시사하고 있다. 향후 제빵공 천식으로 인한 질병부담을 보다 정확히 파악하기 위해서는 국내에서 연구된 바 없는 밀가루 취급 사업장(제분회사 등)에 대한 연구가 필요하며,근로자의 건강과 사회경제적 위치를 보호하기 위한 체계적인 연구도 요구된다. Objectives: To determine the prevalence of baker's asthma due to flour dust in an industrial bakery in Korea. Methods: Of the 596 active workers, 380 were allocated to a category of either direct exposure (DE) or indirect exposure (IE) to flour dust and completed a modified ISAAC questionnaire. Of the 380 participants, 233 were screened by an occupational physician interview, a skin prick test, and an ELISA for serum-specific IgE antibodies to wheat flour extracts. Selected workers received serial monitoring of peak expiratory flow rate (PEFR), and positive PEFR respondents received a methacholine challenge test. The diagnosis of baker's asthma was confirmed by a specific inhalation challenge with wheat flour extracts. Results: The prevalence of sensitization to wheat flour and baker's asthma was 21.0% (25.9% DE, 13.3% IE) and 3.2% (5.1 % DE, 1.8% IE), respectively. DE had an increased risk of sensitization to wheat flour (OR 2.15, 95% CI = 1.03∼4.51). Sensitization to wheat flour and the prevalence of baker's asthma correlated significantly (PR 38.63, 95% CI = 6.14∼243.22). Conclusions: These findings indicate a considerable prevalence of baker's asthma among flour-exposed workers in Korea. Future studies will be required to survey the situation in flour mill companies and seek ways to protect the health and socioeconomic position of flour industry workers.

생활원예 행태에 관한 연구 : 천안시를 중심으로

이애경,도경민,서정근 韓國植物ㆍ人間ㆍ環境學會 1999 인간식물환경학회지 Vol.2 No.3

This study was conducted to investigated the status of horticultural working of Cheonan, Korea. Most of the house had flowers and vegetables garden which was located of gate along the wall and external house. Most of horticultural working participants was women(66%) and age brackets was above 50(72%). The space of horticultural working was internal house(38%) and external house(12%). Cultivated plants in garden were mainly vegetables(30.1%), deciduous tree(25.7%), and evergreen tree(13.5%), plants were cultivated in vacant area(40%) and in weekend farm(16%). Participants were satisfied in their garden, but they required the knowledge of horticultural working(51.9%), So participants of horticultural working required horticultural knowledge, skill and programs by a specialist or governments.

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

의미 치매 1예 : 신경심리 및 뇌영상 검사의 연속적인 추적 관찰 소견 Serial Follow-up Study of Neuropsychological and Neuroimaging Findings

박경원,김욱주,전진민,강도영,김재우 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Semantic dementia (SD) is a very rare, distinct form of dementia which is characterized by impairment of naming and comprehension, prosopagnosia, visual agnosia for objects and relative preservation of other aspects of cognition, such as episodic memory, nonverbal problem-solving and visuospatial functions. A 68-year-old man visited our clinic for progressive speech disturbance since 1997. His main speech problems were word finding difficulty and loss of word meaning for objects. Language assessment demonstrated profound semantic memory loss for words with preserved fluency, corresponding to anomic aphasia. There were marked impairments in verbal and visual memory functions and word fluency tasks, but no grammatical or phonologic errors in speech. Also were remarkable anosognosia and object visual agnosia with preserved visuospatial function and calculation in neuropsychological tests. Serial follow-up study of language test showed more deterioration in comprehension and repetition, consistent with Wernicke's aphasia. Initial brain MR imaging and brain perfusion SPECT showed prominent focal atrophy in the left temporal lobe, and marked hypoperfusion in the left temporal and both frontal areas. Follow-up brain MR imaging and perfusion SPECT revealed more atrophy in both temporal lobes and showed more extensive hypoperfusion areas compared to the previous study.