Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

數種抽出液의 Polychlorinated biphenyls中毒 Rat의 解毒效果에 關한 硏究

정기화 德成女子大學校 1987 德成女大論文集 Vol.16 No.-

The effect of water extract of Asparagus cochinchinensis(A.C), Polygonatum officinale(P.O), Poria cocos(P.C), Panax ginseng(P.G) or Acanthopanax sessiliflorum(A.S) on toxicity of Polychlorinated biphenyls(PCBs) were exanined and following results were obtained. PCBs, an environmental pollutant was administered intraperitoneally to rats.(100㎎/㎏) 1. Rats which were fed PCBs showed decreased of body weight, while the weight of liver was increased in comparison with that of control group. However, these rats when adminidtered of water extract of A.C., P.O., P.C., P.G. or A.S. showed signficantly increase in body weight and recovery of liver weight. 2. Rats which were fed PCBs showed decrease of red blood cells(RBC), white blood cells(WBC), hemoglobin(Hgb) content and gematocrit(Het) value. However these rats when administered water extract of A.C., P.O., P.C., P.G. or A.S. showed recovery in RBC, WBC, Hgb, Het value. 3. Serum cholesterol level and total lipids in rats were also hightened due to the toxicity of PCBs. However the administration of water extract of A.C., P.O., P.C., P.G. or A.S. resulted in lowering of serum cholesterol level and total lipids. 4. All the enzyme were markedly increased in PCBs-intoxicated rats. The activities of serum GOT, GPt and ALP were significantly stimulated by PCBs and their stimulation was inhibited by the administration of water extract of A.C., P.O., P.C., P.G. or A.S.

예방의학적 생리활성물질의 일반약리작용

정기화 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1

누룩치, 고본, 당귀 및 산초의 ethanol ext.는 자발운동량에 미치는 영향에 대한 시험에서 아무런 영향을 미치지 않았다. 다만 phenobarbital sodium 수면시간에 대해서는 산초투여군에서만 수면시간을 다소 단축시켰다. 0.7% acetic acid의 복강내 투여에 의해 유발된 writhing syndrome을 다소 억제하였으나 유의성은 없었다. 체온에 미치는 영향에 대한 시험에서는 유의성 있는 체온 변화가 없었다. 다만 흰쥐의 위액분비에 미치는 영향에 대한 시험에서는 누룩치와 고본투여군에서 위액분비량과 총산도가 유의적으로 감소하였다. 소장운동능에 미치는 영향에 대한 시험결과 누룩치, 당귀 및 고본투여군에서 유의성 있는 charcoal의 이동억제가 관찰되었다. 결론적으로 누룩치, 고본, 당귀 및 산초의 ethanol ext.는 일반약리작용에 대하여 특이한 작용이 없었다. In the study of general pharmacological action, we tested effects on spontaneous momentum, phenobarbital sleeping times, secretion of gastric juice, body temperature and movement of small intestine, etc. Using Pleurospermum kamtschaticum, Angelica tenuissima, Angelica gigas and Zanthoxylum schinifolium as samples to estimate adverse effects. In the study of general pharmacological action to estimate adverse effects, we observed no side effects of sample-treated groups against the control group.

配合藥物의 生物藥劑學的 硏究(V)

鄭淇化 德成女子大學校 1977 德成女大論文集 Vol.5-6 No.-

Sulpyrine by its oral administration with combined Diphen-hydramine HCl were examined by using the improved Writhing wyndrome method, and the following effects were found. 1. When Sulpyrine by its administration with cmobined Diphenhydramine HCl, the ED50 of Sulpyrine with Diphenhydramine HCl is 10 mg/Kg with 90mg/Kg, 15mg/Kg with 85mg/Kg, 20mg/Kg with 80mg/Kg, 25mg/Kg with 75mg/Kg, 30mg/Kg with 70mg/Kg, 35mg/Kg with 65mg/Kg, 40mg/Kg with 60mg/Kg and its effecacy was correspond to 100mg/Kg administration of Sulpyrine alone. 2. When Dipnennydramine HCl was used in assistant, the accive intensity was increased out has no effect in administration alone.

市販食用油의 變質에 關한 硏究

鄭淇化 德成女子大學校 1980 德成女大論文集 Vol.9 No.-

I compared the rate of rancidity by heating to the 2 kinds of marketed edible oil(sample A, sample B), and controlled these samples by adding the anti-oxidant. 1) Rate of rancidity between sample A and sample B appeared considerable gap; generally, sample B was lower. 2) Acid value increased rapidly about 6 hours after, peroxide value and carbonyl value were 2 hours after. 3) Addition of anti-oxidant resisted the rate of rancidity and it was more notable as time goes by.

Polychlorinated Biphenyls(PCBs)의 최근 연구동향과 독성에 관한 고찰

정기화 德成女子大學校 藥學硏究所 2002 藥學論文誌 Vol.13 No.1

Polychlorinated biphenyls(PCBs), and other halogenated aromatic(HA) compounds such as polychlorinated dibenzodioxin(PCDDs) and polychlorinated dibenzofurans(PCDFs), are highly lipophilic and persistent global comtaminants. These compounds are present in air, water, fish, and wildlife, as well as in human blood, adipose tissue, and milk. Exposure to PCBs can cause hepatotoxicity, immunotoxicity, and neurotoxicity. Adverse effects of PCBs have also been observed on reproduction, development, and endocrine function including thyroid hormone homeostasis and estrogen-responsive tissues. The toxic effects of PCBs depend on their degree of chlorination and pattern of chlorine substitution. The biological properties of the most potent (coplanar) PCBs are mediated through the aryl hydrocarbon receptor(AhR) mechanism, but this mechanism represents only a limited number of TCDD-like PCBs congeners. A large proportion of PCBs in the environmental are non-planar or ortho-substituted PCBs(O-PCBs) that have chlorines at the ortho position in the biphenyl ring. These PCBs act through AhR-independent mechanism. They are present in the environment and food chain in relatively higher proportions than TCDD-like congeners, but have been ignored because of their subtle effects. Recent studies have shown that O-PCBs can affect endocrine function, including thyroid hormone homeostasis and estrogen-responsive tissues. In vitro studies have shown that O-PCBs 95 was the most potent in altering intracellular calcium(Ca^+2) regulation through a ryanodine receptor mediated mechanism. Thyroid hormones and steroids play an essential role in normal body homeostasis. Exposure to PCBs and related compounds cause reductions in thyroid hormones in developing and adult animals. O-PCBs have profiles of enzyme, hormone, neurotransmitter, and other biological effects different from those of coplanar PCBs. Most of the O-PCBs are rapidly metabolized by mammals, but not all the O-PCBs are labile. Little information is available regarding the ability of these congeners to affect the hypothalamo-pituitary-thyroid(HPT) axis and estrogen responsive tissue. Moreover, the mechanism(s) of actions of O-PCBs are poorly defined and not completely understood. This review was done to investigate the trend of recent studies and toxicities of PCBs

五加皮 水浸 Extract의 藥里作用

鄭淇化 德成女子大學校 1982 德成女大論文集 Vol.11 No.-

I produced follosing results by Acanthopanax chiicanensis NaKai water extract to the antifatigue effect. 1. When I injected hexobarbital subcutaneously, the order of intensity was; water extract 100㎎/㎏ group, 75㎎/㎏ group, 50㎎/㎏ group, 25㎎/㎏ group. 2. When I injected pentothal subcutaneoulsy, the order of intensity was; water extract 100㎎/㎏ group, 50㎎/㎏ group, 75㎎/㎏ group, 25㎎/㎏ group. 3. When I injected cocaine subcutaneously, the order of intensity was; water extract 100㎎/㎏ group, 75㎎/㎏ group, 50㎎/㎏ group, 25㎎/㎏ group. 4. When I administrated meprobamate orally, the order of intensity was; water extract 100㎎/㎏ group, 75㎎/㎏ group, 50㎎/㎏ group, 25㎎/㎏ group.

Pd/titania 촉매에 의한 아세틸렌 Cyclotrimerization

정용화,이태진,김기석 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.1

Pd/titania 촉매에 의한 아세틸렌 cyclotrimerization의 반응성과 생성물 분포의 변화를 상압조건하의 373K에서 flow reaction 실험에 의해서 연구하였다. 주요 생성물로서 에틸렌과 벤젠이 얻어졌으나 시간이 경과함에 따라서 촉매의 피독으로 인하여 아세틸렌 전화율이 급격히 감소하였다. 일정한 반응기체 유속(공간속도 4,000hr^(-1))에서 아세틸렌 농도를 50, 25, 12.5 vol%로 감소시켰을 경우 벤젠 생성량은 감소하였으나 촉매의 피독은 완화되었다. 아세틸렌 농도 변화에 대한 벤젠 생성량의 비교에 의해서 벤젠 생성반응이 아세틸렌 농도에 대해 1차 반응임을 확인하였다. 또한 일정한 아세틸렌 농도 (25 vol%)에 대하여 공간속도를 4,000 hr^(-1)에서 6,000 hr^(-1)로 증가시켰을 경우 촉매의 피독이 완화되었으며 벤젠 생성량도 증가하였다. Flow reaction에 의한 아세틸렌 cyclotrimerization에 있어서 촉매 피독을 완화시키고 벤젠 수율을 증대시키기 위해서는 반응기체 흐름의 아세틸렌 농도와 유속의 최적화가 필요해 보인다. 본 연구에 있어서 palladium-titania 계면의 oxygen anion vacancoies와 환원된 titania 표면이 종전의 연구자들 보다 100K 낮은 온도에서의 벤젠 생성에 기여한 것으로 추정된다. Cyclotrimerization of acetylene was studied by means of atmospheric flow reaction at 373 K on Pd/titania catalyst to investigate variations of catalytic reactivity and product distribution. While ethylene and benzene were obtained as the major products, rapid decrease in acetylene conversion resulted from catalyst poisoning with the lapse of reaction time. For a fixed flow rate(space velocity 4,000hr^(-1)), as acetylene concentrations decreased from 50 to 25, to 12.5 vol%, benzene yields decreased, whereas catalyst poisoning was attenuated. Comparison of benzene evolution signals from the variation of acetylene concentrations showed that the rate of benzene formation is first-order in acetylene pressure. In addition, for a fixed concentration(25 vol%) of acetylene, as space velocity increased from 4,000 to 6,000hr^(-1), benzene yields also increased and catalyst poisoning was attenuated. Optimization of acetylene concentration and flow rate may be necessary to accomplish the attenuation of catalyst poisoning as well as enhancement of benzene yield from acetylene cyclotrimerization by flow reaction. It can be deduced from this study that oxygen anion vacancies at palladium-titania interface and reduced titania made contributions toward catalytic activity for benzene formation at the temperature 100 K lower than those reported by previous researchers.

여성 청각 ·언어장애인의 취업욕구에 관한 조사 연구 : 부산 ·경남지역을 중심으로

김기태,박병현,서화정 釜山大學校 社會福祉硏究所 1999 社會福祉硏究 Vol.9 No.-