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Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq
( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.
Design of 2-DOF decoupled large stroke precision positioning platform
Hua-wei Ji,Bo Lv,Tian-yi Li,Fan Yang,An-qi Qi,Xin Wu,Jing Ni 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12
Aiming at the problem that the output displacement of the piezoelectric actuator is small and the coupling motion of the positioning platform affects the positioning accuracy, we designed a 2-DOF decoupling large-stroke precision positioning platform. First, a dynamic model based on the lumped mass method was established and verified by simulation. The multi-objective genetic optimization algorithm was used to optimize the structural size parameters of the positioning platform, and the optimal solution set of the structural size parameters of the positioning platform was obtained. Finally, according to the theoretical and simulation results, a prototype was fabricated and the working stroke, decoupling performance and natural frequency were tested experimentally. The results show the coupling rates between axes of the positioning platform in the x-direction and y-direction are 1.31 % and 1.62 %, respectively, the natural frequency is 337.2 Hz, and the positioning stroke is 89.2 μm×85.9 μm. The positioning platform designed in this paper is decoupled and has a large output stroke.
Ze-Hua Zhao,Feng-Zhi Xin,Yaqian Xue,Zhimin Hu,Yamei Han,Fengguang Ma,Da Zhou,Xiao-Lin Liu,Aoyuan Cui,Zhengshuai Liu,Yuxiao Liu,Jing Gao,Qin Pan,Yu Li,Jian-Gao Fan 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Microbial metabolites have emerged as critical components that mediate the metabolic effects of the gut microbiota. Here, we show that indole-3-propionic acid (IPA), a tryptophan metabolite produced by gut bacteria, is a potent anti-non-alcoholic steatohepatitis (NASH) microbial metabolite. Here, we demonstrate that administration of IPA modulates the microbiota composition in the gut and inhibits microbial dysbiosis in rats fed a high-fat diet. IPA induces the expression of tight junction proteins, such as ZO-1 and Occludin, and maintains intestinal epithelium homeostasis, leading to a reduction in plasma endotoxin levels. Interestingly, IPA inhibits NF-κB signaling and reduces the levels of proinflammatory cytokines, such as TNFα, IL-1β, and IL-6, in response to endotoxin in macrophages to repress hepatic inflammation and liver injury. Moreover, IPA is sufficient to inhibit the expression of fibrogenic and collagen genes and attenuate diet-induced NASH phenotypes. The beneficial effects of IPA on the liver are likely mediated through inhibiting the production of endotoxin in the gut. These findings suggest a protective role of IPA in the control of metabolism and uncover the gut microbiome and liver cross-talk in regulating the intestinal microenvironment and liver pathology via a novel dietary nutrient metabolite. IPA may provide a new therapeutic strategy for treating NASH.
Anti-allergic Effects of Caffeine in an Allergic Rhinitis Mouse Model
Yan Jing Fan,Chun Hua Piao,Thi Van Nguyen,Zhen Nan Yu,Ok Hee Chai,Chang Ho Song 대한체질인류학회 2020 대한체질인류학회지 Vol.33 No.1
Caffeine (1, 3, 7-trimethylxanthine) is one of the most widely consumed pharmacologically active products worldwide. Caffeine exhibits various pharmacological activities in central nervous, cardiovascular, and respiratory systems. Additionally, caffeine exhibited anti-inflammatory effects in lipopolysaccharide-challenged rats. However, to our knowledge, the potential anti-inflammatory activity of caffeine in allergic rhinitis (AR) has not yet been investigated. Therefore, in this study, we aimed to examine the anti-allergic effects of caffeine in ovalbumin (OVA)-induced AR in mice. We showed that caffeine attenuated the nasal symptoms, including rubbing and sneezing. It reduced the thickness of the nasal mucosa and alleviated goblet cell hyperplasia in the nasal mucosa. In addition, caffeine ameliorated the inflammation in the lungs and decreased OVA-specific immunoglobulin E (IgE) and IgG1 levels in the serum. It also reduced T helper type 2 (Th2) cytokine (interleukin (IL)-4 and IL-5) levels and elevated Th1 cytokine (IL-12 and IFN-γ) levels in the nasal lavage fluid. Collectively, we suggest that caffeine might have therapeutic effects in AR owing to its anti-inflammatory activities.
Anti-allergic Effect of Fructus amomi on Ovalbumin-induced Asthma Mice Model
Chun Hua Piao,Yan Jing Fan,Thi Van Nguyen,Zhen Nan Yu,Hee Soon Shin,Chang Ho Song,Ok Hee Chai 대한체질인류학회 2021 대한체질인류학회지 Vol.34 No.2
Despite studies on the anti-inflammatory effect of Fructus amomi, mature fruit of Amomum villosum Lour, have been getting increasing extensively, it remains unknown about the detailed effects of F. amomi on ovalbumin (OVA)-induced mouse asthma model. In this study, we examined the effect of F. amomi on OVA-induced asthma by analyzing Th1/Th2 cytokine production, histopathologic changes, and focusing on the NF-κB signaling. Oral administration of F. amomi reduced the number of inflammatory cells especially eosinophils and improved airway and pulmonary inflammation in the lungs of OVA-challenged mice. In addition, F. amomi significantly downregulated the overproduction of tumor necrosis factor-α, IL-1β, IL-4, IL-5 and the levels of OVA-specific both IgE and IgG1 and increased the secretion of interferon-γ and OVA-specific IgG2a. Moreover, F. amomi suppressed the increase of total NF-κB level and the phosphorylation of IκB-α and NF-κB by OVA. F. amomi may have therapeutic effect for allergic asthma by modulating Th1/Th2 cytokine imbalance and inhibiting NF-κB signaling activation.
Allium hookeri Inhibits Inflammation in OVA-induced Allergic Rhinitis in Mice via NF-κB Pathway
Yan Jing Fan,Juan Jin,Chun Hua Piao,Thi Van Nguyen,김유석,채옥희,송창호 대한체질인류학회 2023 해부·생물인류학 (Anat Biol Anthropol) Vol.36 No.3
Allium hookeri (A. hookeri) has been used as a traditional medicine for many years in the Southeast Asian region. Multiple investigations have demonstrated its role in preventing of microbial infection, immune regulation, coronary thrombosis, and atherosclerosis has been demonstrated by several studies. Therefore, this study aims to investigate the suppressing effect of A. hookeri on the ovalbumin (OVA)-induced allergic rhinitis (AR) murine model. In this study, AR was induced in BALB/c mice by using OVA emulsified in aluminum on days 1, 8 and 15. Then, nasally challenged with OVA on days 22 to 28. On days 16 to 28, OVA-induced mice were treated either with A. hookeri (50, 100, 200 mg/kg) or Dexamethasone (Dex, 2.5 mg/kg) by oral gavage. On days 22 to 28, mice received either A. hookeri (50, 100, 200 mg/kg) or Dexamethasone (Dex, 2.5 mg/kg) an hour before the OVA challenge. The mice in the control group were not sensitized, treated, or tested. In this study, A. hookeri ameliorated nasal symptoms include rubbing and sneezing; reduced thickness of nasal mucosa; alleviated goblet cell hyperplasia and eosinophil infiltration in the nasal mucosa; decreased levels of OVA-specific IgE and OVA-specific IgG1 in serum; and suppressed Th2 (IL-4, IL-5, and IL-13) cytokines level in nasal lavage fluid (NALF). In addition, A. hookeri recovered the production of Th1 (IL-12 and IFN-γ) cytokines level in NALF, inhibited the inflammation through NF-κB pathway. Collectively, our results suggest that A. hookeri effectively protects against AR by regulating Th1/Th2 cytokines imbalance and NF-κB mechanisms. These observations suggest A. hookeri as a potential therapeutic option for AR.