肺炎 誘發菌의 生育을 抑制하는 韓藥材 探索에 關한 硏究

정병운,서운교,정지천,한영환 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

韓藥材 중 肺炎의 치료효과가 기대되는 淸熱化痰, 止咳平喘藥을 중심으로 23種의 藥材를 사용하여 肺炎을 유발하는 K. pneumoniae, S. pyogenes 및 S. pneumoniae 세균의 생육을 억제하는 藥材를 탐색하고, 탐색된 藥材의 추출물에 대한 세균의 最小生育抑制濃度를 측정하였다. 韓藥材의 水溶性 추출물 중 黃連, 胡黃蓮, 敗醬, 黃芩이 K. pneumoniae에서, 黃連이 S. pyogenes에 대해서 생육억제 효과가 있었으며, S. pneumoniae에 대한 比較沮止環의 直徑은 黃連, 鳥梅, 五味子, 黃芩이 우수하였으나 黃連 추출물 사용시 병원균 모두에서 生育沮止環의 크기가 가장 크게 나타났으며 對照群로 사용된 大腸菌과 枯草菌에서도 어느 정도의 抗細菌 효과를 볼 수 있었다. 에탄올 추출물에서는 敗醬, 黃芩, 五味子, 鳥梅가 K. pneumoniae 세균의 생육을 억제하였으며, S. pyogenes 세균은 黃連, 五味子, 鳥梅, 馬兜鈴찾, 黃芩에 의하여 생육이 억제되어 黃連은 水溶性 및 에탄올 溶解性 주출물에서 모두 우수한 抗細菌 효과를 보여주고 있다. 각 세균에 대한 最小生育沮止濃度(MIC)는 K. pneumoniae 세균일 경우 黃連 및 黃芩의 水溶性 추출물과 鳥梅 및 敗醬의 에탄올 溶解性 추출물이 유의성을 보였으며, S. pyogenes 세균은 黃連의 水溶性 및 에탄올 溶解性 추출물과 鳥梅의 에탄올 溶解性 추출물, S. pneumoniae 세균은 黃連과 鳥梅의 水溶性 및 에탄올 溶解性 주출물에서 유의성이 나타났다. 이상의 결과를 통하여 黃連, 五味子, 鳥梅, 黃芩, 胡黃蓮, 敗醬의 水溶性 및 에탄올 溶解性 추출물이 肺炎 誘發菌f K. pneumoniae, S. pyogenes, S. pneumoniae에 對하여 우수한 生育抑制 作用을 나타냄을 알 수 있었다. The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against pulmonary disease-causing Klebsiella pneumoniae, Streptococcus pyogenes, and Streptococcus pneumoniae. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extracts of Coptis japonica, Scutellaria baicalensis and Picrorrhiza kurroca showed the antibacterial activity against K. pneumoniae and that of C. japonica against S. pyogenes. The antibacterial activities of C. japonica, Prunus mume, Schizandra chinesis, Scutellaria baicalensis were also found against S. pyogenes. When C. japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little activity against B. subtilis and E. coli as a control. 2. The ethanol-soluble extracts of Patrinia scabriosaefolia, P. mume, S. baicalensis, S. chinensis showed the antibacterial activity against K. pneumoniae and those of S. baicalensis, C. japonica, S. chinensis P. mume against S. pyogenes and S. pneumoniae. However, those extract showed a little antibacterial activity against B. subttilis and E. coli except for that the extract of C. japonica showed comparatively high growth inhibition of B. subtilis. 3. Among the medicinal herbs tested, the water- and ethanol extracts of C. japonica showed very extcellent antibacterial activity against the pathogenic bacteria and controls. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against K. pneumoniae were 10 ㎎/㎖ and 22 ㎎/㎖, respectively. The MICs of the ethanol-soluble extracts of P. mume and P. scabriosaefolia were 5 ㎎/㎖ and 20 ㎎/㎖, respectively. 5. For the MICs against S. pyogenes, C. japonica showed 15 ㎎/㎖ with the water-soluble extract and P. mume and C. japonica with the ethanol-souble extract did 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. 6. For the MICs against S. pneumoniae, C. japonica and P. mume with the water- and ethanol-souble extract showed 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. As a result, the highest antibacterial activity was found in the water- and ethanol-soluble extracts of C. Japonica against pulmonary disease-causing bacteria, K. pneumoniae, S. pyogenes, and S. pneumoniae. Also, the water- and ethanol-soluble extracts of S, chinensis, P. mume, S. baicalensis and P. kurrooa showed high antibacterial activities.

三和散이 家兎 腎臟機能에 미치는 影響

鄭智天 東國大學校韓醫學硏究所 1992 東國韓醫學硏究所論文集 Vol.1 No.-

In order to examine that the effect of Sam Hwa San, circulating the vital energy of Sam Cho and controlling body fluid metabolism, gives any influence on renal function, changes in the urine flow. eletrolytes excretion, plasma aldosterone concentration and renin activity were observed after intravenous infusion of the Sam Hwa San extract in rabbit. Also in vitro enect of the herb extract on oxygen consumption in rental cortical slices and ATPase activity in kidney microsomese was measured. The following results were obtained: 1. The urine flow was markedly increased at 10 min after intravenous infusion of the Sam Hwa San extract(0.134 ±0.015 vs. 0.043±0.046 ml/min.kg), but return ed to normal value after 40 min of infusion. 2. The glomerular filtration rate was significantly increased at 10 min after in travenous infusion of the Sam Hwa San extract, following return to normal value. 3. Na+ excretion was significantly increased during 10-40 min after intravenous infusion of the Sam Hwa San extract, although showed the maximal rate at 10-20 min. The fractional Na+ excretion was also increased during 10-40 min. K+ excretion was rapidly increased at 10 min after the intravenous Infusion of the Sam Hwa San extract and then gradually decreased to normal level at 40 min. The fractional K+ excretion was significantly increased during 10-40 min after the intravenous infusion of the Sam Hwa San extract. 4. The plasma aldosterone concentration and renin activity were altered by the infusion of the Sam Hwa San extract. 5. The ouabain-sensitive oxygen consumption of renal cortical slices was signify cantly reduced by the Sam Hwa San extract(0.5 and 1.0 vol.%). 6. The Na-K-ATPase activity of renal microsomes was strongly inhibited by the Sam Hwa San extract(0.5 and 1.0 vol.%). These results suggest that the Sam Hwa San causes a strong diuretic effect which results from reduction of Na reabsorption in renal tubule by a direct inhibition of Na-pump and, in part, from am increase in renal blood flow. In clinic, it is considered to obtain the therapeutic effect in body fluid metabolism disharmony to cause the circular disorder of vital energy.

엄지손가락 과다증을 동반한 수열에서 이엽피판을 이용한 제 1 갈퀴막 공간의 재건

문재원,천지선,양정열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Cleft hand is a rare disease that is characterized by absence of one or more digits, It was introduced by Abroise Pare in 1575. The cause of this is not identified yet. Polydactyly is defined as normally pentadactylous hand with excess digits, It was reported that the possible cause of polydactyly is increased folding of AER(apical ectodermal ridge). There are so rare cases that cleft hand is accompanied with preaxial polydactyly. We experienced a case of cleft hand with preaxial polydactyly. That case had narrowing of first web space and thumb polydactyly. Untilil now, there have been so many methods of reconstruction of web such as skin graft, z-plasty, dorsal and volar flap, etc. We reconstruct the first web with bilobed flap elevated from polydactylized thumb. As it has good results of functional and cosmetic properties, It's very useful to use accessory tissue from polydactylized finger such as our case.

Cytokine interleukin-1β로 誘發된 糖尿病 마우스의 膵臟 glucokinase 및 hexokinase 活性에 對한 五倍子의 效果에 關한 硏究

최형일,정지천,김철호 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.7 No.1

Glucokinase와 hexokinase는 간과 췌장에서 인슐린에 의해 혈당이용을 증대시키며 혈당농도를 감지하여 인슐린 분비를 조절하는 효소로서 인슐린비의존성당뇨병(NIDDM)의 병인과 밀접한 관계가 있는 효소이다. Cytokine의 일종인 interleukin-1β (IL-1β)는 췌장 도세포에서의 인슐린 분비와 β-cell에 대한 세포 독성을 효과적으로 조절하여 장기간 노출시키면 인슐린 분비와 생합성을 저해하며 β-cell을 파괴하여 β-cell의 기능적, 구조적 손상을 초래하여 IDDM을 유발한다. 본 연구에서는 당뇨병 치료의 전통한방약제로서 사용되고 있는 五倍子추출물의 효과를 해명하기 위하여 IL-1β유발 실험적 당뇨마우스의 glucokinase와 hexokinase의 활성을 검토한 결과, lL-1β주사에 의해 체중과 공복시의 glucose 및 insulin 분비가 증가되었으나, 五倍子투여로 공복시의 insulin분비가 감소하였으며 체중과 공복시의 혈당은 유의성은 없었으나 대조군에 비하여 감소하였다. 그러나, 혈당수치가 정상군에 비하여 현저하게 증가되었으나 五倍子투여로 현저히 감소하였으며 Insulin치의 상승과 분비지연도 五倍子투여군에서는 정상군과 유사한 경향을 보였다. 한편, 혈중 glucokinase와 hexokinase의 활성은 IL-1β주사에 의해 현저히 감소되었으나, 五倍子 투여로 유의성 있게 상승되었다. 이상의 결과로, 五倍子는 IL-1β로 유발된 실험적 당뇨병에서 포도당인산화 효소인 glucokinase와 hexokinase의 활성을 증가시키는 것으로 나타났다. We investigated the in vivo effect of an aquous extract from Rhois Galla (R-G) on glucokinase and hexokinase activities of diabetes mellitus induced by interleukin-β (IL-1β). After 1 week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of R-G, IL-1β injection allowed the scrum glucose level increased and the level was decreased by R-G administration. Furthermore, we could observe that R-G was effective in recovering the levels of insulin secretion. Enzyme activities of the gtucokinase and hexokinase were decreased by IL-1β treatment. In contrast, R-G administration to the mice allowed proportion increasing. Seemingly, when IL-1β was injected to the mice, enzyme activities of the glucokinase and hexokinase were decreased. But, R-G stimulated induction of enzyme activities of the glucokinase and hexokinase as high as normal group. These results suggested that R-G is highly effective in treatment of diabetes mellitus.

萬金湯 및 加味萬金湯이 高脂血症에 미치는 影響

서운규,정지천,朴宣東 東國大學校韓醫學硏究所 1994 東國韓醫學硏究所論文集 Vol.3 No.-

In order to investigate the effect of Mangumtang(MT) and Gamimangumtang(GMT) experi-mental studies were performed in Ex.1(treated with cholesterol and administration of sample at a time) and Ex2.(administration of sample after the treatment of cholesterol). The results were summerized as follows : 1.The content of Total cholesterol in the serum was not significantly decreased in MT group, whereas in GMT group sinificantly decreased compared with control group in Ex.1 and all significnatly decreased in MT group and GMT group in Ex.2. 2.The content of Triglyseride in the serum was all significantly decreased in MT group and GMT group in all case. 3.The content of Phospholipid in the serum was not significantly decreased in MT group, whereas in GMT group was significantly decreased compared with control gruop in Ex.1 or Ex.2. 4.The content of Free Fatty Acid in the serum was significantly decreased in MT group and GMT group in all case. 5.The content of HDL-Cholesterol in the serum was tended to decreased compared with control group but did not show a significance in Ex.2 or Ex.2. 6.The content of LDL-Cholesterol in the serum was not significantly decreased in MT group and GMT group in Ex.1, whereas in Ex.2 not significantly decreased in MT group but significantly decreased in GMT group. According to the above results, it is assumed that Mangumtang and Gamimangumtang have a vaild effect on Hyperlipidemia. especially Gamimangu-mtang have a more valild effect on prevention and cure of Hyperlipidemia.

升麻葛根湯 및 升麻葛根湯合四物湯의 抗 Histamine 效果에 關한 硏究

金賢兒,鄭智天,朴宣東 동국대학교 한의학연구소 1994 東國韓醫學硏究所論文集 Vol.3 No.-

In order to find out the effect of Anti-Histamine of the Sungmagalkuntang and Sungmagalkuntang-plus-samultang I've investigated the effect of the time to death, the peripheral vascular Peaneability, the amount of secretion of Gastric Juice, the total Acidity of Gastric Juice, the contraction of Intestine Tube by Histarnine and the effect of the Skin-Reaction by the decrease of DNCB. These results are following 1. The effect of Mice s rime to death by Histamine was that the injection group of sungmagalkuiigtang and the injection group of sungmagalkungtang-plus-samultang both have had the effect of the extention uf the time lo death, which indicated a statistical significance in comparison with comparative group. 2. The effect of the increased vascular permeability by Histamine was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang both have had the effect of the restraint of the increased vascular permeability, which indicated a statistical significance in comparison with comparative group. 3. The effect of the the amount of the secretion of Gastric Juice by Histamine stimulus was tirat the injection group of sungmagalkuntang-plus-samultang only has had the effect of restraint of the increased of the secretion of Gastric Juice, which indicated a statistical significance in comparison with comparative group. 4. The effect of the chan,ge of total Acidity of Gastric Juice by Histamine's stimulus was that the injection group of sungmagalkuntang-plus-samultang only has had the effect of the restraint of the increase of tolal Acidity of Gastric Juice,, which indicated a statistical significance in comparison with comparative group. 5. The effect of the contraction of Intestine Tube by Histamine was that sungmagalkuntang and sungmagalkuntang-plus-samultang both have had the effect of the contraction and the restraint According to the increase of the density, the effect of the restraint was more clear. But the difference of the effects between two substances had little. 6. The effect of the reaction of skin by the decrease of DNCB was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang have had the decreased effect of the Skin-Reaction in comparison with comparative group. sungmagalkun-tang-plus-samultang aws more effective. In these results shown above, both sungmagalkuntang and sungmagalkuntang-plus-samultang was shown as more effective one

하악골 골절수술에서 흡수성 골고정장치의 유용성

문재원,천지선,양정열 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Introduction: The mandible is one of frequent facial bone fracture due to anatomic configuration like the nose and zygoma. When the mandible fractures occured, displacement of segments developed by pulling of strong muscles. And so for reduction of fractured mandible, rigid fixation needs for prevention of displacement. Metal plates and screws are most appropriate device to obtain for prevention of displacement in case of mandible fracture. But metal devices are hampered by temperature sensitivity and interference with radio-graphic imaging, leading corrosion, inflammatory response, the effect of atrophy of cortical bone, the need for a subsequent operation for removal. To overcome drawbacks of metal rigid fixation devices, there had been a continuous research on the development of a bioabsorbable skeletal fixation system using polymers of polylactic and polyglycolic acid. Material and Method: One hundred sixty eight fractures of the mandible in 110 patients (84 male patients and 26 female patients) were treated by open reduction and internal fixation using bioabsorbable plates and screws (BiosorbFX^(R), Bionix Implants, Inc., Finland). The average age is 36.5 years old. Total number used absorbable devices and 972 (182 plates and 790 screws). Patients were evaluated during the follow-up period, which ranged from 1 to 30 months. Common fracture sites are para symphysis (26%), body (19%) and angle (18%) which are experienced. Intermaxillary fixation placed before operation but removed after internal fixation of absorbable devices. After 5 days from operation, temporomandibular joint movement are started. We encountered no significant intraoperative difficulties obtaining placement of the devices. Result: None of patients has been experienced any implant-related complication including fracture instability or relapse, or radiographic evidence of osteolysis except one case of delayed osteomyelitis and four cases of intraoral infections. Conclusion: Absorbable devices have many advantage, that are lower infection rates, satisfactory strength, no metallic irritation, no restrictive growth of the neurocranium in pediatric patients, only one surginal procedure. Absorbable devices have some problems, such as slow and expense. But we experienced good results using absorbable devices to mandible fracture, and these results indicate that the use of bioabsorbable implants can be considered routinely for the fixation of mandible fractures.

枇杷葉의 抗糖尿病藥理活性과 IL-1β誘發糖尿病 마우스의 膵臟 燐酸化酵素에 미치는 效果에 關한 硏究

윤철호,정지천,김철호 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.7 No.1

淸凉生津, 解渴上嘔의 효능이 있으며, 한국등에서 항당뇨병성 한약으로서 사용되어 온 비파엽의 열수출물의 당뇨병치료효과를 검증하기 위하여 alloxan으로 유발된 당뇨병흰쥐의 혈장포도당농도 및 인슈린농도를 측정하였다. 비파엽처리군의 혈장 포도당농도는 현저히 증가하였으며 혈장인슈린농도는 크게 감소하지 않았다. 한편, 당뇨병유발의 내재적인 요소로 알려진 사이토카안인 IL-1β로 당뇨병을 유발한 뒤 그 효과를 검정했다. 즉, 실험동물을 interleukin-1β로 처리하여 실험적으로 당뇨병을 유발한 후 glucose의 燐酸化에 관여하는 glutokinase와 hexokinase의 활성을 살펴보았다. 그 결과, 비파엽엑기스는 interleukin-1β 과잉투여 당뇨병으로 인하여 증가된 혈액내 glucose농도를 감소시키고 insulin 과잉분비를 정상으로 회복시키는데 효과적이었다. 동시에 lL-1β에 의해 유도된 당뇨마우스에 대한 枇杷葉엑기스 投與가 췌장 glucokinase와 hexokinase의 활성을 촉진하는 것으로 나타나 lL-1β유발 당뇨병에 효과가 있는 것으로 사료되었다. Studies were conducted on anti-dibetic effect of the water extract from leaves of Eriobotryae folium which had been used in Korea as a remedy for dibetes. The extract was found to inhibit the Increase in the plasma level of sugar bu the not the decrease in the plasma level of insulin in alloxan-induced dibetic rata. Also, we investigated the in vivo effect of an aqueous extract(referred to as EF) from Eriobotryae folium on glucokinase and hexokinase activities of diabetes mellilus induced by Interteukin-1β (IL-1β). After 1 week of IL-1β injection, the levels of serum glucose concentration and insulin secretion were dramatically increased. However, the insulin secretion was decreased with administration of EF. The level of glucose concentration was decreased by EF administration. Furthermore, it was observed that EF was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase, which are key enzymes of glucose phosphorylastion, were decreased by IL-1β. EF administration to the mice allowed proportional increasing by stimulation of induction of enzyme activities as high as normal group. These results suggested that EF is highly effective in treatment of diabetes mellitus induce by IL-1β.

Melanoma B-16 에서 IL-1β 유발 GnT-Ⅲ,ⅴ 활성에 대한 瓜蔞仁 , 黃芩 추출물의 影響

徐雲敎,鄭智天 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

The activity of N-acetylglucosamitnyltransferase(GnT) Ⅲ and V on a Melanoma B-16 was examined after incubation with interleukin 1 (IL-l ). While augumatting cell proliferation, IL-1 resulted in a decrease of GnT-Ⅲ activity and an increase of GnT-V activities. Consist-u with this, Mdamnu B-16 cultured with IL-l showcd increased affinlity to Daturam stramonium latin, which raogfuzes asialo-tri- and asialeo-tetra-antenery N-linked oligosaccharides. These results indicate that IL-1 modulate glycosyloaderse activity and the oligosaccharide sVtuture of target cells. On the omer hand, to investigate whether or not TKM-SG affect GnT-V gene expression in lung metastatic carcinoma, we used RT-PCR methods. TKM-SG treated cell lines showed low leve1s of secretion of GnT-V mRNA transcription·as elucidated by RT-PCR. Thus, with together lower GnT-V acnuty levels in the medium, TKM-SG was highly enative for lung cancer metastasis treatment and it was concluded that the medicine can be used as a potent anti-lung cancer metastasis medidcine.

鹿茸 藥針 製劑가 힌쥐 腎臟 組織의 抗酸化 作用에 미치는 影響

윤철호,정지천,신억섭 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

Cervus elaphus for herb-acupuncture solution (CEHAS) was tested for the effects of free radical generating enzyme and lipid peroxidation in rat's kidney. In vitro, levels of lipid peroxide in tissues of kidney were proportionally decreased to concentration of extracts prepared from CEHAS. They were much more decreased, when lipid perocidation was inducesd with ferrous iron(Fe II). Also, enzyme activities of xanthine oxidase were decreased. The ratio of type conversion of zanthine oxidase was lowered, too. But, it was not seen changes on enzyme activities of aldehyde oxidase. These results suggest that CEHAS decrease the activities of free radical generating enzymes such as xanthine oxidase which form lipid peroxide.