ROSAE MULTIFLORAE RADIX의 藥理作用에 關한 硏究

殷載淳,韓宗鉉,崔動晟 又石大學校 1989 論文集 Vol.11 No.-

In an attempt to investigate the effect of ROSAE MULTIFLORAE RADIX(RME) on the diabetes in rats induced by alloxan(80 ㎎/㎏) or streptozotocin(40 ㎎/㎏) and the contractile force of isolated rabbit ileum, RME was administered per oral daily and the level of blood glucose, serum triglyceride and serum total cholesterol were measured by spectrophotometer and the contractile force of intestine was measured by physiograph. The following resuits were obtained: 1) RME did not change the level of blood glucose and serum total cholesterol, but decrease the serum triglyceirde on the 5th day in normal rats. 2) The level of blood glucose in diabetic rats induced by the alloxan was decreased on the 5th and 7th day of experiment (RME 100㎎/㎏), induced by streptozotocin was decreased on the 7th day of experiment (RME 10 ㎎/㎏) and 5th and 7th day of experiment (RME 100 ㎎/㎏). 3) The level of serum triglyceride in dibetic rats induced by the alloxan was decreased 7th day (RME 100 ㎎/㎏) and induced by streptozotocin was decreased 7th day (RME 10 ㎎/㎏) and 3th, 5th and 7th day (RME 100 ㎎/㎏) of experiment. 4) The level of serum total cholesterol in diabetic rats induced by the alloxan and streptozotocin was not changed by RME. 5) The contractile response of isolated rabbit ileum by RME was not affected by cyproheptadine, but inhibited by atropine and verapamil.

사군자탕이 L1210 세포를 이식한 마우스의 면역세포에 미치는 영향

殷載淳,金大根,柳東和,權鎭,徐龍勳,蘇俊魯,全焄,吳贊鎬 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

The purpose of this research was to investigate effects of Sa-Kunja-Tang(SKT) on immune cells of L1210 cell-transplanted mice. The apoptosis and T lymphocytes subopoulation were tested using a flow cytometry, and the proliferation was tested using a MTT assay. Nitric oxide production from mouse peritoneal macrophage was tested using a Griess reagents, and the phagocytic activity of mouse peritoneal macrophage was tested using a lucigenin chemiluminescence. SKT suppressed apoptosis of T-lymphocytes induced by L1210 transplantation. SKT decreased nitric oxide production from mice peritoneal macrophages increased by L1210 transplantation, and the phagocytic activity decreased by L1210 transplantation. These results suggest that SKT suppresses T lymphocyte apoptosis and macrophage activity in L1210 transplanted mice.

청피에 함유된 복강 파크로파지의 탐식작용 억제 성분

은재순,김대근,소준노,지옥표 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The phagocytic activity of murine peritoneal macrophage was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles. The activity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis, lsosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

3T3-L1 세포주에서 분비하는 인체 암세포 성장억제 단백질에 대한 연구

은재순,권진 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Ingibition of the growth of human cancer cells by proteins secreted from 3T3-L1 cells was investigated in the present study. The growth of human cancer cells was inhibited by co-culture with 3T3-L1 cells under 10% FBS and DME, GIT and serumless medium, respectively. The conditioned medium of cultured 3T3-L1 cells under serumless medium was concentrated 100-fold through an ultrafiltration cell with a 10,000 molecular weight cutoff at 4℃ under positive pressure using nitrogen (3T3-L1 EM). 3T3-L1 EM inhabited the growth of HrRa, Hep G2, KHOS-Np, A431 andMCF-7 cells. 3T3-L1 EM was purified with FPLC, DEAE-ion exchange chromatography and pheny-sepharose chromatography. The major protein of 3T3-L1 EM has a molecular weight of 66,000-69,000 in SDS-PAGE analysis. The results suggest that the inhibitory activity of 3T3-L1 EM appears to be due to some protein(m.w. 66,000-68,000) secreted by 3T3-L1 cells.

複合生藥製劑의 止血作用및 摘出子官筋에 미치는 影響(第4) : 壽脾煎 및 歸脾양에 대하여 On Soo-Bi-Jeon and Kwi-Bi-Tang

殷載淳,李東熙,黃甲洙 又石大學校 1990 論文集 Vol.12 No.-

These experiments were conducted to investigate the pharmacological difference between Soo-Bi-Jeon and Kwi-Bi-Tang extract,, clinically used in gynecology, on the hemostasis and the contractile force of the isolated uterine muscle. For this purpose, the effects of the extracts on the bleeding time in mouse tail and prothrombin time in vitro were estimated. Forthermore, its activity on the isolated uterine muscle in rats were investigated. The results obtained were as following; The bleeding time and prothrombin time were significantly shortened compared with the control group in all samples, The uterotonic action produced by Kwi-Bi-Tang(Sample II), Sample III and IV (the same component crude drugs between not blocked by atropine (10^(-7)M) and cyproheptadine (10^(-7)M). but inlhibited by pretreatment of verapamil (10^(-7)M). On the oter hand, Soo-Bi-Jeon(Sample I) extract relaxed the uterine muscle.

複合生藥製劑의 止血作用및 摘出 子宮筋에 미치는 影響(第3報) : 加減當歸補血湯

殷載淳,廉思俊,韓宗鉉 又石大學校 1989 論文集 Vol.11 No.-

These experiments were performed to investigate the effects of the KAGAM-DANGKWI-BOHYUL TANG(KDBT) extract, clinically used in gynecology, on the hemostatis and on the contractile force of the isolated uterine muscle. The bleeding time and the plasma prothrombin time test were employed as the means to estimate the hemostatic effect of KDBT extract, and the contractile effect of the extract was measured. The experimental results were summarized as followings; 1. In mice treated with KDBT extract the plasm prothrombin time and the bleeding time were remarkably shortened compared with the control group. 2. The contractile effect of KDBT extract on the isolated uterine muscle was not blocked by atropine and cyproheptadine. 3. The contractile effect of KDBT extract on the isolated uterine muscle was inhibited by the pretreatment with verapamil. From these experimental results, it is concluded that the hemostatic effect of KDBT extract might be attributed to the stimulation of prothrombin formation and the direct contraction of the uterine muscle through the increasing uptake of calcium ions into the muscle.

자궁암세포(HeLa)에 대한 사군자탕과 수종 항암제의 병용투여 효과

은재순,김현욱,소준노,오찬호,이송재 又石大學校 1993 論文集 Vol.15 No.-

The studies were conducted to investigate the combined effects of Sa Kunja Tang(SKT) and several anti-cancer drugs. The effects of SKT and several anti-cancer drugs on the proliferation of HeLa cells, human cervical cell line, was estimated by MTT colorimetric assays. The SKT extract inhibited the proliferation of HeLa cell at 10^-4g/ml. The inhibitory action of the mitomycin C(MMC), mercaptopurine(MCP) and 5-fluorouracil(5-Fu) treated group, respectively, on the proliferation of HeLa cell was increased by the SKT. When the mice were treated by the MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of the MMC treated with the SKT, the number of leukocytes was increased significantly than the group of the MMC treated only at the 3rd day. The SKT extract decreased the thymus weight of mice. The SKT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and the SKT increased the number of PFC significantly than the MMC treated group. The SKT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and the SKT increased T cell proliferation significantly than the MMC treated group. In conclusion, the results presented in this paper suggest that the SKT extract can decrease the dose of anti-cancer drugs on HeLa cell, and recover the side dffects of the MMC, such as leukopenia and immunosuppresion, without any intercalating the anti-proliferative action of the MMC in vivo.

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

사군자탕이 항암제를 투여한 마우스의 면역세포에 미치는 영향

殷載淳,金大根,柳東和,權鎭,洪鍾星,蘇俊魯,全焄,吳贊鎬 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

The purpose of this research was to investigate effects of Sa-Kunja-Tang(SKT) on immune cells of antitumor drugs administered mice. The apoptosis and T lymphocytes subpopulation were tested using a flow cytometry, and the proliferation was tested using a MTT assay. The administration of etoposide. vincristine or doxorubicin increased the apoptosis of T-lymphocytes, but the action of doxorubicin was decreased by the administration of SKT. The administration of etoposide or vincristine decreased helper T and cytotoxic T cells population of T lymphocytes, but the action of vincristine was recovered by the administration of SCT. The administration of etoposide, vincristine or doxorubicin decreased the proliferation of T-lymphocytes, but the action of doxorubicin was increased by the administration of SKT. These results suggest that SKT has a regulative function of T-lymphocytes in anti-tumor drugs administered mice.

Cantharis의 세포독성

殷載淳,權鎭,全焄,吳贊鎬,蘇俊魯 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The cytotoxicity of cantharidine(CTD), the main component of Cantharis, and the combined effect of CTD and anti-tumor drugs on HeLa. Hep G2,SK-OV3. KOHS-NP, BALB/c 3T3 cells, mouse splenocytes and human lymphocytes were estiated by MTT colorimetry assay. CTD inhibited the proliferation of anti-tumor cells, BALB/C 3T3, mouse spleen cells and human lymphocytes. CTD increased the cytotoxicity of mitomycin C, cisplatin or mercaptopurine on tumor cells and BALB/c 3T3 cells. These results indicate that cantharidine has the cytotoxicity of tumor cells, fibroblast cells and immunocytes.