Cycloheximide가 膵臟腺房細胞에 미치는 影響에 關한 微細形態學的 硏究

徐仁壽,洪錫宰 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

蛋白合成障碍가 膵臟腺房細胞의 微細構造 및 自家貪食力에 미치는 影響을 알아보기 爲해 흰쥐에 cycloheximide와 自家貪食亢進劑인 neutral red를 單獨 또 倂合 投與하여 惹起된 微細形態學的 變化를 電子顯微鏡으로 觀察하였던 바를 要約하면 다음과 같다. Cycloheximide는 膵臟腺房細胞에 比較的 初期에서부터 Golgi complex의 腫脹, ER膜에 附着된 ribosome의 脫落을 主로 한 輕한 變化를 일으켰다. 60分이 經過하면 ER 및 mitochondria의 輕한 腫脹, 脫落된 ribosome의 破壞 및 消失等을 볼 수 있었으나 그 程度는 輕微하였다. Autophagic vacuole의 計數는 對照群이 한 腺房當 10.1±2.2個, neutral red投與群이 54.4±4.9個, cycloheximide와 neutral red를 同時에 投與한 群에서는 21.5±4.1개, cycloheximide投與 30分 後 neutral red 投與群에서는 9.7±2.7個이었다. 이와 같은 事實은 cycloheximide가 autophagic vacuole形成에 抑制的 效果가 있다는 것을 意味하며 autophagic vacuole의 形成에는 어떤 形態로든 새로운 蛋白合成이 必要하다는 것을 示唆하는 것이라 생각된다. This study was carried out to investigate the effects of cycloheximide, known as an inhibitor of protein synthesis at the level of polypetide elongation, on the fine structures of pancreatic acinar cells. Also studied was the effects of protein synthesis inhibition by cycloheximide on the neutral red-induced autophagocytosis. Twenty-one male albino rats, maintained on water only for 12 hours, weighing 200㎎. in average, were divided into 5 groups, 3 rats for each groups except Group 2. Group 1: This is to serve as a control group. Two consecutive doses of 0.4㎖. of saline per 100 gm. of body weight were given intraperitoneally to 3 rats at interval of 30 minutes. Group 2: A dose of 2 mg. of cycloheximide per 100gm. of body weight was given to 9 rats intraperitoneally and 3 animals each were sacrificed 30, 60 and 90 minutes after injection, respectively. Group 3: A dose of 2mg. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitoneal injection of 0.4 ㎖ of saline per gm. of body weight. Group 4: A dose of 2㎎. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitioneal injection of 2㎎. of cycloheximide per 100gm. of body weight. Group 5: Simultaneous intraperitoneal injection of 2 ㎎. of neutral red and 2㎎. of cycloheximide per 100gm. of body weight was given to 3 rats. All animals were sacrificed 60 minutes after the the last injection except Group 2. Pancreas extirpated for both light and electron microscopic studies. The results were summarized as follow: Cycloheximide induced moderate dilatation of Golgi complex aad detachment of ribosomes from RER membrane from early stage (sacrificed 30 minute after injection). Mild swelling of RER and mitochondria along with destruction and disappearance of detached ribosomes were noted in the animals sacrificed 60 and 90 minutes after injection (Group 2). The number of autophagic vacules per acinus was 10.1±2.2 in the control group (Group 1); 54.4±4.9 in neutral red only group (Group 3); 21.5±4.1 in neutral red simultaneous with cycloheximide group(Group 5); 9.7±2.7 in the group with neutural red administered 30 minutes after cycloheximide injection(Group 4). The results showed marked inhibitory effects of cycloheximide on the neutral red-induced autophagocytosis, suggesting need for do novo synthesis of protein in the formation of the autophagic vacuoles.

信用카드去來의 法律問題에 관한 硏究

徐圭錫,尹洪善 全北大學校 1991 論文集 Vol.33 No.-

In modern socity credit card became the necessities of our daily life as a consumer. The first form of credit card was appeared in the United States and it is said that the pioneer of credit card was Hotel Credit Company. Subsequently, a full-scale usage of credit card had to be on the lookout the flotation of cards by oil company about 1914. In 1950, Diners' Club was sailed as a company specialiaing in credit card, it was the departure of the 'tripartite' card and about 1958. 10, the issue of American Express Card brought about the universal use of oredit card in consumer transactions. In our country the credit card was first introduced by Shynsegae Department Store in 1969. 10, thereafter Midopa Department store issued credit card and it was not until Kookmin-card was brought out by Koomin Bank in 1980. 9 that credit card was diffused to us in earnest. But the wide-spread usage of credit card has raised problems on consumer protection and rational restriotions of credit card transactions terms. Besides, our operative law on credit card does not give us entire satisfactions as regards the insufficiency of provisions and the drastic protection for comsumer. That is to say, both the bipartite and tripartite cards create legal problems when the card has been lost or stolen and an unauthorized person has used the card to make purchases before the holder notified the issuer in writing that the card had disappeared. This study focused on these problems and put emphasis on the solution of consumer protection problems. The substances of this thesis are as follows; 1. an introduction 2. the history and conception of credit card 1) the history of credit card and the development process of credit card in various countries. 2) the conception of credit card 3) the kind of credit card 4) the legal nature of credit card 3. the legal structure of credit card transactions 1) the parties 2) the legal relations of parties 4. the legal nature of relations on price payment 1) an introduction 2) theories of USA 3) theories of BRD 4) theories of Japan and Korea 5. a conclusion

정신보건법의 주요 쟁점 조항에 대한 선진국과의 사례 비교

홍진표,황순찬,박수빈,서동우,정은기,김진학,박종익,안주연,김수정,장홍석 大韓神經精神醫學會 2009 신경정신의학 Vol.48 No.2

Objectives The Mental Health Act is an initiative aimed at changing and shaping mental health services and protecting human rights of persons with mental disorders. Since the Mental Health Act was legislated in 1995, four amendments have been made according to the issues that arose from public concerns. However, there are still many debates about the human rights protection of the mentally iII. This study aimed to provide information regarding major aspects of the Mental Health Act by comparing them among several developed countries. Methods Current Mental Health Acts of the state of Michigan in the United States, Scotland in England, the state of Victoria in Australia, and Japan were reviewed. Issues regarding the Korean Mental Health Act were collected from seminar materials, news media contents, and mental health professionals Results The definition of subjects in Korean Mental Health Act was more inclusive than other countries and was derived from a medical classification of mental illness. Family members or guardians were granted important responsibilities for deciding the involuntary admission of mentally ill patients in Korea and Japan. In Western countries, Mental Health Review Tribunals or courts have the primary responsibility for important decisions about mentally ill patients. The regulation of immediate dis- charge after request by voluntarily admitted patients was not enacted in all countries except Korea. The mandatory procedure for involuntary admission in Western countries includes an individual case review with personal interview by a Mental Health Review Tribunal or court. Conclusion The Korean Mental Health Act appears to meet the basic standards of Guidelines from international organizations. Our traditional culture and inherent health systems seem to influence the legal regulation of mental health service and might be related to the problems of human rights protection of mentally ill patients in Korea.

FWD를 이용한 콘크리트 덧씌우기 줄눈부에서의 하중전달효과 연구

양홍석,서영찬 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

콘크리트포장의 구조적 능력을 평가하는 가장 중요한 요소 중의 하나는 하중전달효과이다. 하중전달효과는 양쪽 슬래브의 처짐량비로 결정된다. 하중전달효과가 낮은 슬래브에서는 하중이 한쪽 슬래브에만 부하되어 콘크리트포장의 파괴를 촉진시키고 포장수명을 단축시킨다. 본 연구에서는 슬래브 온도, 다우월바 시공유무, 줄눈틈이 하중전달효과에 미치는 영향을 알아보기 위해 88 고속도로에서 FWD(Falling Weight Deflectometer)를 이용하여 다양한 온도조건에서 하중 전달효과 조사틀 실시하였다. 연구결과 다우월바를 시공한 구간에서는 온도변화에 관계없이 하중전달효과가 큰 차이를 보이지 않은 반면, 다우월바를 시공하지 않은 구간에서는 저온에서 하중전달효과 감소가 컸다. 또한 다우월바를 시공한 구간이라도 포장이 노후화되면 하중전달효과는 온도가 하락함에 따라 감소하는 것으로 나타났다. 본 조사대상 구간에서는 슬래브 단위온도 하락시 하중전달효과는 2.6% 감소하는 것으로 나타났다. One of the most important factors accounting for the structural capacity of concrete pavement is load transfer. Load transfer efficiency is determined by measuring deflection ratio across the slabs. In joints where load transfer efficiency is low, stress is concentrated in the loaded slab and accelerates the deterioration of the concrete, reducing the life of the pavement. In this study load transfer efficiencies were measured at various temperature conditions on 88 Express Highway using FWD(Falling Weight Deflectometer) in order to find the effects of slab temperature, construction of dowel bars, crack width at the joint on the load transfer efficiency. For the sections with dowel bars, there were little differences in load transfer efficiency regardless of temperature changes. For the sections without dowel bars, however, there were great losses of load transfer efficiency at low temperatures. For the old pavement, even in the sections with dowel bars, the load transfer efficiency reduced as the temperature dropped. For the sections in this study, 2.6% reduction of the load transfer efficiency was observed for each 1 ℃ drop in the slab temperature.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

활동성 궤양성 대장염 환자에서 발생한 뇌정맥 혈전증 1예

남승우,양석균,정훈용,주연호,홍원선,민영일,김종성,유창식,서대철 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

한국인의 식품 영양조사 : 제3보

安富浩,柳彦浩,鄭碩敎,金乙祥,洪性愛,徐德揆 한림대학교의료원 1977 人間科學 Vol.1 No.1

난치성 만성정신분열증 환자의 생활의 질에 미치는 정신사회치료 프로그램의 효과에 대한 예비연구

송동호,배민진,이종호,이홍식,김선경,서호석,김찬형,전지용 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.4

Objects : Recent studies of psychosocial adjustment after hospitalization have found that the combination of maintenance antipsychotic drug treatment and psychosocial treatment including psychoeducational program are highly predictive of social rehabilitation and reduction of subsequent relapse. Two groups of patients with refractory chronic schizophrenia were preliminarily compared to determine the effect of a psychosocial treatment program on the quality of life in refractory chronic schizophrenics in an open comparative trial. Methods : One group(N=11) was assigned to approximately six months of the psychosocial treatment program(including psychoeducation program and activity program such as interpersonal relationship program, social skill training, self management program, outings, etc), in a group format, twice a week and a fixed maintenance dosage of clozapine ; while scale(QLS) was used to provide an objective measure of changes in patient's psychosocial functioning and a general assessment of psychopathology was made using the Brief Psychiatric Rating Scale(BPRS). Results : Both BPRS total positive score and the QLS total score, especially in the intrapsychic foundation factor of the scale showed a statistically significant improvement in the psychosocial treatment group. But there was no significant change in both BPRS and QLS scores over a 6-month period in the non-psychosocial treatment group. A significant negative correlation was found between the negative symptom and changes of QLS total, instrumental role and common object and activities scores respectively after receiving a 6-month of the psychosocial treatment program. Conclusion : These results suggest that a psychosocial treatment program including the integration of psychoeducation and a skill training oriented activity program serve as an outpatient treatment modality to improve the quality of the life in refractory chronic schizophrenia. To further clarify the effect of psychosocial treatment in chronic schizophrenia, a randomized trial should be done.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

Original Article : Impact of immunosuppressant therapy on early recurrence of hepatocellular carcinoma after liver transplantation

( Ju Yeun Lee ),( Yul Hee Kim ),( Nam Joon Yi ),( Hyang Sook Kim ),( Hye Suk Lee ),( Byung Koo Lee ),( Hye Young Kim ),( Young Rok Choi ),( Geun Hong ),( Kwang Woong Lee ),( Yung Suk Suh4 ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.2

Background/Aims: The most commonly used immunosuppressant therapy after liver transplantation (LT) is a combination of tacrolimus and steroid. Basiliximab induction has recently been introduced; however, the most appropriate immunosuppression for hepatocellular carcinoma (HCC) patients after LT is still debated. Methods: Ninety-three LT recipients with HCC who took tacrolimus and steroids as major immunosuppressants were included. Induction with basiliximab was implemented in 43 patients (46.2%). Mycophenolate mofetil (MMF) was added to reduce the tacrolimus dosage (n=28, 30.1%). The 1-year tacrolimus exposure level was 7.2 ± 1.3 ng/mL (mean ± SD). Results: The 1- and 3-year recurrence rates of HCC were 12.9% and 19.4%, respectively. Tacrolimus exposure, cumulative steroid dosages, and MMF dosages had no impact on HCC recurrence. Induction therapy with basiliximab, high alpha fetoprotein (AFP; >400 ng/mL) and protein induced by vitamin K absence/antagonist-II (PIVKA-II; >100 mAU/mL) levels, and microvascular invasion were significant risk factors for 1-year recurrence (P<0.05). High AFP and PIVKA-II levels, and positive 18fluoro-2-deoxy-d-glucose positron-emission tomography findings were significantly associated with 3-year recurrence (P<0.05). Conclusions: Induction therapy with basiliximab, a strong immunosuppressant, may have a negative impact with respect to early HCC recurrence (i.e., within 1 year) in high-risk patients. (Clin Mol Hepatol 2014;20:192-203)