케일녹즙이 고콜레스테롤식이 흰쥐의 혈청 및 간장의 지질성분에 미치는 영향

박필숙,강진순,정승용,박재옥,김행자 慶尙大學校 1987 論文集 Vol.26 No.1

체중 60~65㎏의 wistar계 숫쥐에게 cholesterol 무첨가식이에 물과 kale 녹즙을 각각 급여하여 혈청중 총 cholesterol 농도, HDL-cholesterol 농도, 간장중의 총 cholesterol 농도, 혈청 및 간장중의 TG와 PL의 농도 그리고 과산화지질 농도를 측정하여 다음과 같은 결과를 얻었다. 1) 혈청중 총 cholesterol 농도는 cholesterol 무첨가식이군인 1,2군산 cholesterol 첨가이식군인 3,4, 5군간에서 각각 유의성이 없었다. HDL-cholesterol 농도는 cholesterol 무첨가식이군인 1,2군과cholesterol 첨가이식이군인 3,4,5군에서 kale 녹즙을 급여한 군이 유의적으로 높았다. 2)혈청의 중성지방농도는 전 군간에 유의성은 없으나 물ㅇㄹ 급여한 군보다 kale 녹즙을급여한 군에 있어 약간 낮은 경향이었다. 혈청의 인지질 농도는 cholesterol 무첨가식이군 및 cholesterol 첨가이식군 모두 물을 급여한 군보다 kale 녹즙을 급여한 군이 유의적으로 높았다. 3)간장의 총 cholesterol 농도는 cholesterol 첨가식이군에 있어 물을 급여한 군이 약간 낮은 경향이었다. 4)간장의 중성지방농도는 cholesterol 무첨가식이군에 비해 cholesterol 첨가이식군인 3,4,5군이 유의적으로 높았으며, 이들 군간에 있어서는 kale 녹즙혼액군이 비교적 낮았다. 간장 인지질농도는 cholesterol 첨가식이군인 3,4,5군간에 있어 kale 녹즙과 녹즙혼액을 급여한 3,4,군이 비교적 낮았다. 5)혈청 및 간장의 과산화지질농도는 cholesterol 첨가이식군인 3,4,5군이 무첨가식이군에 비해 대체로 낮았으며 또한 kale녹즙과 녹즙혼액을 급여한 군에 있어 유의적으로 낮았다. 이상의 결과로 보아 kale 녹즙의 섭취는 혈청 HDL-cholesterol 농도의 상승효과가 있고 간장 중 cholesterol 및 인지질의 농도를 다소나마 저하시키며 또한 혈청및 간장의 과산화지질농도의 지하작요이 있으므로서 혈청 및 간장의 지질개선효과가 있는 것으로 사료된다. The effect of kale juice, Brassica oleracea, on lpid components in serum and liver were investigated by feeding male rats of wistar for four weeks. And the concentrations of T-cholesterol,phospholipid, triglyceride and lipid peroxide in serum and liver were analysed along with the HDL-cholesterol concentration of serum . The results were as follows: 1)There was not found ny significant influence on the concentration of T-cholesterol in serum, butsome of relatively higher inluence but some of relatively higher influence on the HDL-cholesterol concentration. 2)For the group fed with 100% kale juice the concentration of triglyceride was shown lower than for any other groups, while that of phospholipid was shown higher. 3)The concentration T-cholesterol in liver was shown a little lower for the kale juice groups. 4)The concentration of triglyceride in liver was remarkably decreased in the 100% kale juice group in comparison with other groups, whiel the phospholipid concentraion was not. 5)Lipid peroxide values in serum and liver were relatively decreased in the kale juice groups compared to other group.

도농간 교육격차 해소를 위한 농촌지역 유형별 교육혁신 추진과제 탐색

나승일,정철영,구자억,박행모,장호순,김진구,마상진,조단비,문세연 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구는 교육혁신에 대한 이론적 고찰을 토대로 농촌지역 교육혁신 성공요소를 도출하고, 농촌의 지역특성을 고려하여 도농간 교육격차 해소를 위한 농촌지역 유형별 추진과제를 제시하는 데 목적이 있었다. 연구목적 달성을 위해 문헌고찰 및 사례연구를 실시하였고, 연구결과의 타당성 확보를 위해 전문가협의회를 실시하였다. 농촌지역 교육혁신 성공요소는 마을공부방 운영 등 26개가 최종적으로 도출되었다. 또한 농촌지역 교육혁신모형을 개발하여 목표와 전략을 수립하고, 이를 기초로 농촌지역 유형별 추진과제를 제시하였다. 농촌지역 교육혁신의 비전은 "도농간 교육격차 해소를 위한 교육혁신 실현으로 살고 싶은 농촌지역 구현" 으로 설정하고, 이를 달성하기 위한 목표는 도시수준의 교육기회 제공, 농촌형 수월성 교육 추구, 지역 인적자원개발로 설정하였다. 농촌지역 유형별 중점적으로 추진해야 할 과제는 발전 농촌지역은 마을 공부방 운영 및 학습도우미 지원, 중간수준 농촌지역은 교육환경 개선, 장학금 지원 및 다양한 교육기회 제공, 저개발 농촌지역은 기존 지원 유지, 적극적 학습 지원 및 교원의 근무여건 개선, 도농복합 농촌지역에서는 교통편의 제공, 학습기회 개선 및 학부모의 교육참여 유도 등이 선정되었다. 그리고 농촌지역 유형별 중점 추진과제에 대한 단계별 로드맵과 이를 적용하기 위한 정책적 제언을 제시하였다. The purpose of this research was to derive success factors and explore actions plan for the education innovation of rural areas for releasing education gaps between urban and rural areas. For the purpose, the researcher carried out literature reviews and case analysis were conducted, and experts council were executed. As a result, 26 success factors for the education innovation of rural area were derived. And, this study suggested the education innovation model of rural area including goals and strategies to identify action plan for the education innovation by types of rural areas. The vision of the education innovation model was making the place pleasant to live in by doing education innovation for releasing education gaps between urban and rural areas. The goals of the education innovation model were to provide urban-level educational opportunities, to pursue rural-customized education for excellency and to develope local human resources. This study identified several core tasks corresponding to each rural area: additional establishment and operation of village study-rooms and support for learning assistant system for highly-developed rural areas; improvement of educational condition and provision of scholarship and various educational opportunities for middle-developed rural areas; maintenance of existing support, active learning-support and improvement of teachers' working conditions for lowly-developed rural areas; provision of traffic convenience, improvement of learning opportunities and promotion of parents' educational participation for urban-rural complex areas. Moreover, this study suggested roadmap according to action plan divided by types of rural areas, and political proposal for effective actualization of that.

Notes on bird species newly recorded in Korea

Kim, Hwa-Jung,Kim, Dong-Won,Hur, Wee-Haeng,Jang, Byoungsoon,Lee, Yun-Kyung,Kwon, In-Ki,Park, Jong-Hyun,Park, Jong-Gil,Kang, Chang-Wan,Kim, Eun-Mi,Choi, Soon-Kyoo,Kim, Han-Kyu The National Institute of Biological Resources 2020 Journal of species research Vol.9 No.4

A total of 527 species of birds have been recorded in Korea. We summarize the records of three genera and six species that have been newly reported in Korea, discovered through the avian monitoring programs carried out by The National Institute of Biological Resources from 2013 to 2016. These six newly recorded species are; Ring-necked Duck (Aythya collaris), Lesser Scaup (Aythya affinis), Bufflehead (Bucephala albeola), Black-winged Kite (Elanus caeruleus), Mongolian Lark (Melanocorypha mongolica) and Crested Myna (Acridotheres cristatellus). One Ring-necked Duck was observed on March 23, 2014 in Jungrangcheon Stream. One male Lesser Scaup was recorded on February 24, 2014 in Gyeongpoho Lagoon. A male bufflehead was first seen in Yeongrangho Lagoon on January 26, 2013. The Black-winged Kite was first observed on January 23, 2013 at the Gangseo Wetland Ecological Park. One Mongolian Lark was also recorded on May 11, 2016 and May 12, 2017 in Marado Island. The Crested Myna was first observed on April 20, 2016 in Seomando, Island.

Renal dipeptidase and β-Lactam antibiotics

( Haeng Soon Park ) 전남대학교 약품개발연구소 2004 약품개발연구지 Vol.13 No.-

Poster Session 2 : DNA: Replication, recombination, repair and modification ; Cysteins 289 and 486 in Largest subunit of replication protein A regulate its binding activity through redox

( Haeng Soon Jeong ),( Andre Kim ),( Hyun Ju Lim ),( Soon Gi Kang ),( Yung Jin Kim ),( Shin Won Kang ),( Jang Su Park ) 한국생화학분자생물학회 (구 한국생화학회) 2000 추계학술대회집 Vol.2000 No.-

Spontaneous Release of Glycosylphosphatidylinositol (GPI)-anchored Renal Dipeptidase from Porcine Renal Proximal Tubules

Park, Sung-Wook,Kang, Bok-Yun,Yoon, Hyun-Joong,Park, Eun-Mi,Choi, Kyong,Lee, Hwang-Hee Blaise,Hooper, Nigel M.,Park, Haeng-Soon The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.1

The incubation of porcine renal proximal tubules (PTs) resulted in the release of the Glycosylphosphatidylinositol (GPI)-anchored renal dipeptidase (RDPase, EC 3. 4. 13. 19) from the membrane after a lag period of approximately 6 hours. This spontaneous release of RDPase from the membrane was inhibited by antibiotics. When the incubation supernatant was added back to fresh PTs, both the antibiotic inhibition of RDPase release and the lag period disappeared. The released RDPase reacted with an anti-cross reacting determinant antibody indicating the presence of the Ins (1, 2-cyc)P. These results suggest that bacteria in the PTs, when incubated, grow find Secrete a phosphatidylinmsitol-specific phospholipase C (PIPLC). This enzyme then hydrolyses the GPI-anchored RDPase and is transferable. RDPase was purified following its release from the membrane by this simple and inexpensive method which may also be applied to other GPI-anchored proteins.

Urinary Dipeptidase in Reference of Serum Creatinine

Park, Haeng Soon 전남대학교 약품개발연구소 1996 약품개발연구지 Vol.5 No.1

Urinary dispeptidase(Udpase) in reference of serum creatinine of eight patients as acute renal failure (ARF) were compared with the results of three healthy individuals. Specific Activity (S.A.; munit/g blood creatinine) of ARF pests exhibited clear distinction from healthy individuals at the time of hospitalization but increased significantly ac up to the normal level with recovery. It may be used to distinguish between ARF from healthy individuals and to evaluate the efficiency of various treatments in addition to the conventional methods of diagnosis of penal diseases.

Stability of New Carbapenem DA-1131 to Renal Dipeptidase : DehydropeptidaseⅠ

Park, Sung Wook,We, Jeoung Soon,Kim, Gye Won,Choi, Seong Hak,Park, Haeng Soon 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

The stability of DA-1131 to renal dipeptidase (RDPase) (EC 3.4.13.19) was compared with that of imipenem and meropenem by V_max/ K_m ratios as an index of the enzyme's preference for substrates. Our results showed a decreasing order of imipenem (6.24), meropenem (2.41), and DA-1131 (1.39). The biochemical evaluation of DA-1131 as the least preferred substrate of RDPase suggest its potential use as a novel β-lactam antibiotic which may be usable without coadministration of RDPase inhibitors once its clinical suitability is proven.

Glycosyl-Phosphatidylinositol (GPI)-Anchored Renal Dipeptidase Is Released by a Phospholipase C in vivo

Park, Sung Wook,Choi, Kyong,Lee, Hwanghee,Park, Sung Kwang,Turner, Anthony J.,Hooper, Nigel M.,Park, Haeng Soon 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

The release mechanism of the glycosyl-phosphatidylinositol (GPI)-anchored renal dipeptidase (EC 3.4.13.19) in vivo has been investigated. Triton X-114 phase separation indicated that the dipeptidase is exclusively present as a hydrophilic form in urine from porcine, rat, rabbit and human. Western blot analysis of human and porcine purified dipeptidase and the urine concentrates with anti-(cross-reacting determinant) serum demonstrated the presence of inositol 1, 2-cyclic monophosphate indicating that the renal dipeptidase had been released from the membrane by the action of a phospholipase C. This is the first direct evidence for cleavage of a human GPI-anchored protein by a responsible phospholipase C in vivo.

Spontaneous Release of Glycosylphosphatidylinositol (GPI)-anchored Renal Dipeptidase from Porcine Renal Proximal Tubules

Park, Sung Wook,Kang, Bok Yun,Yoon, Hyun Joong,Park, Eun Mi,Choi, Kyong,Lee, Hwanghee Blaise,Hooper, Nigel M.,Park, Haeng Soon 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

The incubation of porcine renal proximal tubules (PTs) resulted in the release of the glycosylphosphatidylinositol (GPI)-anchored renal dipeptidase (RDPase, EC 3. 4. 13. 19) from the membrane after a lag period of approximately 6 hours. This spontaneous release of RDPase from the membrane was inhibited by antibiotics. When the incubation supernatant was added back to fresh PTs, both the antiblotic inhibition of RDPase release and the lag period disappeared. The released RDPase reacted with an anti-cross reacting determinant antibody indicating the presence of the lns (1,2-cyc) P moiety. These results suggest that bacteria in the PTs, when incubated, grow and secrete a phosphatidylinositol-specific phospholipase C (PI-PLC). This enzyme then hydrolyses the GPI-anchored RDPase and is transferable. RDPase was purified following its release from the membrane by this simple and inexpensive method which may also be applied to other GPI-anchored proteins.