흰쥐에서 연령이 골격근의 당 대사에 미치는 영향

정응찬,우운기,이석강 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: It is doubtful that aging causes deteriorated glucose metabolism and insulin resistance of skeletal muscle. Some researchers had different results about it. So we have studied the mechanism responsible for the abnormal glucose tolerance associated with aging in rapidly growing and matured rats. Materials and Methods: Animals were used S.D. rats. Growing rats were 7 weeks old (BW: 160-190 gm) and matured rats were 28 weeks old (BW: 420-525 gm). Results: Fasting blood glucose and plasma insulin levels were significantly elevated in matured rat compared with growing rats. And during oral glucose tolerance test the glucose level was also significantly elevated in matured rats. These results confirmed an insulin resistant state of aging. Insulin levels at 30 minutes of oral glucose tolerance test was significantly elevated in growing rat. But at 120 minutes it was maintained at higher level in matured rats than in growing rats. It suggested the possibility of increased insulin secretion by initial stimulation of beta-cells in growing rats, and increased secretion and decreased catabolic rate of insulin in matured rats. Glucose uptake rate of soleus muscle in matured rats was lower than that of growing rats, but the difference was not statistically significant. The dose(insulin)-responsive(glucose uptake) curve of soleus muscle was only slightly deviated to the right side. Conclusion: Glucose metabolism of rat skeletal muscle was worsened by aging. The data of glucose uptake experiments suggested the possibility of insulin resistance of skeletal muscle in matured rats, but the mechanism of insulin resistance of skeletal muscle need further studies.

고지방식이가 운동시 흰쥐의 글리코겐 이용에 미치는 영향

민경선,장응찬,김형렬,민진아,신덕수 慶山大學校 2000 論文集 Vol.18 No.1

Carbohydrate and fat ingestion during exercise are essential nutrient, but a number of opinion is different from their contents of food. In this point of view, to study how high-fat dietary contents affects glycogen utilization, 48 female Sprague-Dawley rats were trained on the treadmill trained for 4wk with consuming two high-fat diet and one high-carbohydrate diet. All animals were fed 79%high-fat diet(n=16, 79F), 40% high-fat diet(n=16, 40F), 70% high-carbohydrate diet(n=16, 70C). After the final day of training, one-half of rats in each the three groups were run to exhaustion (E), whereas the remaining rats served as rested controls (R). All animals were trained on a motorized rodent treadmill. The animals ran for 30min/d at 28m/min(0% grade), analysis items were glycogen, body weight and %Fat were measured. All data were examined by means of one-way ANOVA and significance was set at the p<0.05. The Duncan method was used for a post hoc test. Body weight were similar among the 79F, 40F and 70C groups. %Fat was significantly greater in animals that consumed the 79F group than in those on the 70C group (P<0.05), even though similar body weight each other. Generally liver and muscle glycogen concentration had more in 79FR, on the other hand, had less in 79FE compared with the others. The mean overall glycogen utilization rate was depressed in muscle and liver after animals were on the 79% F diet. In summary, the present study has suggested that consumption of 79% F diet during endurance training can lead to an increase in exercise performance in female rats. Some of the consequences training on F diet are an increase in body fat deposition and slower utilization of glycogen during exercise. These data indicate that rats exposed to a high-fat diet are capable of prolonging exercise in spite of limited glycogen stores. This improved capacity for exercise appears to be partially the result of muscular adaptations to the diet, which apparently increase the ability to oxidize fat and concomitantly spare glycogen.

씨름선수에서 체중 감량과 회복기의 Leptin과 PAI-1 변화

김용운,도경오,권태동,박덕일,장응찬,박소영,김종연,이석강 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Obesity which is defined as accumulation of excess body fat, is central factor of insulin resistance syndrome. Recently, it is revealed tat adipose tissue is not simply an energy storage organ but it also secretes a variety of molecules which affect the metabolism of the whole body, those are leptin and plasminogen activator inhibitor-1(PAI-1). Therefore, leptin and PAI-1 are increased in the obese state. Leptin regulates energy homeostasis and satiety and PAI-1 regulates fibrinolytic system. For these reasons, elevated levels of leptin and PAI-1 are considered as link factors between obesity and insulin resistance syndrome. However, the exact regulating mechanism for serum levels of leptin and PAI-1 is not fully understood yet. In this study, to evaluate the regulating mechanisms of serum levels of leptin and PAI-1 according to the weight changes, we measured leptin, PAI-1, physical, metabolic, and endocrine parameters during 10 days of weight reduction and 10 days of regain period in 7 young athletes. The mean body weight change was -4.7 kg(5.0%) in the weight reduction period and -2.4 kg(2.5%) in the weight regain period compared to baseline value. Baseline level of leptin in athletes was 1.7±0.66 ng/ml, which was significantly correlated with body weight, BMI, percent body fat, body fat mass, triglyceride, insulin, and PAI-1. Baseline level of PAI-1 in athletes was 16.6±5.26 ng/ml, which was significantly correlated with body weight, BMI, triglyceride, insulin, and leptin. Leptin was decreased to 0.7±0.39(44% of the basaline value) in the weight reduction period, and increased to 1.9±0.64(119% of the baseline value) in the regain period. PAI-1 was decreased to 7.4±2.72(44% of the basaline value) in the weight reduction period, and increased to 22.8±7.33(138% of the baseline value) in the regain period. The changes of leptin during weight reduction period were significantly correlated with the changes of insulin(r=0.890, P<0.01) and triglycerides(r=0.874, P<0.01). The changes of PAI-1 during weight reduction period were significantly correlated with the changes of FFA(r=0.889, P<0.01) and triglycerides(r=0.869, P<0.05). The changes of both leptin and AAI-1 during weight regain period were significantly correlated with the changes of insulin(r=0.755 and 0.849, P<0.05, respectively). In summary, these results suggest that serum levels of leptin and PAI-1 were affected by weight cycling, the percentages of change were more greater than that of weight change, and rebound phenomena were occurred during weight regain period.

흰쥐에서 탄수화물 종류가 운동시 당 및 지질대사에 미치는 영향

권태동,전중기,민경선,김형렬,장응찬 대구대학교 기초과학연구소 1998 基礎科學硏究 Vol.15 No.2

The purpose of this study was to investigate the effects of glucose, sucrose, and glucose polymer(GP) ingestion on the carbohydrate and lipid metabolism during treadmill running in rats. Rats were randomly assigned to normal, control and carbohydrate groups. Carbohydrate group was divided into glucose, sucrose and GP ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either normal saline(control) or carbohydrates solutions of formulated glucose(6%), sucrose(6%) and GP(6%) via polyethlene tube to stomach before treadmill run. Exercise was loaded by a treadmill run at the speed of 1 km/hour for 30, 60 and 120 minutes. The results of experiment were as follows: Plasma glucose levels were decreased in proportion to the exercise time in control, but increased at treadmill run 30 minutes and then gradually decreased with the lapse of the exercise time in carbohydrates compared with normal rats. It decreased in control, glucose, sucrose and GP compared with normal rats in decreasing order at treadmill run 120 minutes(% change of plasma glucose level of control, glucose, sucrose and GP compared with normal rats were 67, 74, 87 and 91 in plasma glucose level at treadmill run 120 min., respectively). Plasma lactate levels were increased by the exercise loading compared with normal in control and carbohydrates ingested rats. It increased in control, sucrose and GP in increasing order compared with normal rats, at treadmill run 120 minutes(% change of plasma lactate level of control, glucose, sucrose and GP compared with normal rats were 167, 185, 149 and 114 in plasma lactate level at treadmill run 120 min., respectively). Plasma FFA levels had tendency to increase by the exercise loading compared with normal in control and carbohydrates ingested rats. It showed increase in the sucrose, glucose, GP and control rats in Increasing order compared with normal rats at treadmill run 120 minutes(% change of plasma FFA level of control, glucose, sucrose and GP compared with normal rats were 151, 153, 166 and 152 in plasma FFA level at treadmill run 120 min., respectively). Plasma acetoacetate levels were higher in control compared with carbohydrates ingested rats at treadmill run 30, 60 and 120 minutes. Plasma beta-hydroxybutyrate levels increased by exercise compared with normal rats in control and carbohydrates ingested rats at treadmill run 30, 60, 120 minutes. Plasma insulin levels were decreased in proportion to the exercise time in control compared with normal rats, but in carbohydrates ingested rats increased at treadmill run 30 minutes and then gradually deceased with the lapse of the exercise time compared with normal. In carbohydrates ingested rats, plasma insulin levies were higher than control. Hematocrit did not show any difference between control and carbohydrates rats at treadmill run 30, 60 and 120 minutes. From these experimental results, it is suggested that the exhaustion of skeletal muscle glycogen, the hypoglycemia, the overproduction of lactate and dehydration is delayed in carbohydrates ingested rats. GP of the carbohydrates is considered as the most effective for these effects. Therefore, intake of carbohydrates before exercise, especially GP seems to increase performance in the prolonged moderate exercise.

운동부하 흰쥐에서 포도당 증합체가 심근 당원에 미치는 영향

이석강,조성현,김용운,박덕일,김종연,장용찬 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.1

운동부하전 섭취한 탄수화물이 운동흰쥐의 심근 및 골격근이 당원량에 미치는 영향을 측정하여 심근과 골격근의 대사적 차이점의 일단을 연구한 본 논문의 결과는 다음과 같다. 심근과 골격근 당원량(mg/ g wet tissue, 이하 단위 동일)은 정상군에서 심근 2.62±0.51로서 골격근의 3.37±0.58보다 낮았다(p<0.01). 심근 당원량은 정상군이 2.62±0.51이었다. 운동 30분에서 대조군은 정상군의 63%수준으로 감소하였으며(p<0.01), 탄수화물섭취군은 정상군의 86 및 90%수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 대조군의 142 및 137%정도로 양군 모두 대조군보다 유의하게(p<0.01) 높았다. 운동 60분에는 대조군은 운동30분의 78%(정상군의 49%)수준으로 감소하였으며(p<0.05) 탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 운동 30분에 비하여 72(p<0.01, 정상군의 62%), 81%정도(p<0.05, 정상군의 73%)로 감소하였다. 포도당 섭취군의 당원량은 대조군의 147% 정도로 대조군보다 유의하게(p<0.01) 높았다. 운동 120분에서 대조군은 운동 60분에 비하여 63%(정상군의 33%) 수준으로 감소하였으며 (p<0.01) 포도당섭취군은 운동 60분의 69%(정상군의 50%)수준으로 유의하게 감소하였으나, 포도당 중합체 섭취군은 차이가 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 202 및 162% 정도로 양군 모두 대조군보다 유의하게(p<0.01)높았다. 골격근 당원량은 정상군이 3.37±0.58 이었다. 운동 30분에서 대조군은 정상군의 69% 수준으로 감소하였으며(p<0.01) 탄수화물섭취군은 포도당 중합체 및 포도당섭취군에서 각각 정상군의 85 및 88% 수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 125 및 129% 정도로 양군 모두 대조군보다 유의하게(p<0.05) 높았다. 운동 60분에는 대조군은 운동 30분의 77%(정상군의 53%) 수준으로 감소하였으며(p<0.05)탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 30분에 비하여 93(정상군의 80%), 89(정상군의 78%) %정도로 감소하였으나 통계적으로 유의하지는 않았다. 포도당중합체 및 포도당 섭취군의 당원량은 각각 대조군의 152 및 149% 정도로 대조군보다 유의하게 (p<0.001) 많았다. 운동 120분에서 대조군은 운동 60분에 비하여 50%(정상군의 26%) 수준으로 감소하였으며(p<0.001) 포도당 섭취군은 운동 60분의 75%(정상군의 58%)수준으로 유의하게(p<0.05) 감소하였으나 포도당 중합체 섭취군은 대조군의 84%(정상군의 67%)정도로 감소하는 경향을 보였다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군우ㅢ 255 및 221% 정도로 양군 모두 대조군보다 유의하게(p<0.001) 높았다. 이상의 결과로 미루어 보아 심근과 골격근 간의 생리, 생화학적 특성의 차이에도 불구하고 탄수화물의 섭취후 중등도 정도의 달리기 운동시 근 당원의 함량에 미치는 영향은 정도의 차이는 있으나 같은 경향을 나타내었으며, 골격근에서 심근보다 더 많은 근 당원 절감효과를 나타내었다. 또한 운동전 탄수화물의 섭취시 이러한 근 당원 절감 효과로 장시간의 운동시 심근 및 골격근 당원의 고갈 지연으로 운동수행능력의 증가가 기대되며, 특히 포도당 보다는 포도당 중합체의 섭취시 효과가 더 크리라고 사료된다. The purpose of this study was to investigate the effect of glucose and glucose plymer ingestion on the concentration of the cardiac muscle glycogen at 30, 60 and 12 minutes of the treadmill running in rats. Rats were randomly assinged to normal, control, and carbohydrate groups. Carbohydrate group was divided into glucose, and glucose polymer ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either water (control) of carbohydrates of formulated glucose(6%), and glucose polymer(6%) via polyethylene tube to the stomaach before treadmill run. Exercise was loaded by a treadmill run at the speed of I ㎞/hr for 30, 60, and 120 minutes. The results of experiment were as follows: Glycogen content of the cardiac muscle was lower than that of the skeletal muscle (plantaris) in normal rats. Glycogen content of the cardiac muscle was decreased in proportion to the exercise time compared to normal in control and carbobydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in decreasing order at 120 minutes treadmill running (% change of glycogen content of control, glucose, and glucose polymer compared to normal rats were 33, 50, and 63% in the cardiac muscle at 120 minutes of treadmill running respectively). Glycogen content of the skeletal muscle was decreased in proportion to the exercise time compared to normal in control and corbohydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in ecreasing order 120 minutes treadmill running(% change of glycogen content of control, glucose, and glucose polymer compared to normal were 26, 58, and 67% in the cardiac muscle at 120 minutes of treadmill running respectively). Change of the glycogen content of the cardiac and the skeletal muscle during treadmill running after glucose and glucose polymer ingestion showed that both muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrates ingested rats. Decreasing rate of glycogen content of cardiac and skeletal muscles during treadmill running compared to normal in carbohydrates ingested rats was lower than that of control. From these results, it is suggested glycogen content of cardiac and skeletal muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrate ingested rats. Glycogen content of cardiac and skeletal muscles in carbohydrate singested rats less decreased than that of control during treadmill running compared to normal. Glucose polymer of the carbohydrates ingested rats in considered as the most effective for glycogen sparing according to exercise time in both muscles. Therefore, intake of carbohydrate, especially glucose polymer, before exercise may be increase performance of the prolonged moderate exercise.

(A)comparative analysis of physical fitness in lean, normal and obese high-school girls

Jang, Eung-chan 慶北大學校出版部 1993 體育學會誌 Vol.21 No.-

97년도 춘계학술대회 : 학술발표 : 운동이 인슐린분비 및 작용에 미치는 영향

장응찬(Jang Eung Chan),이수천 한국운동영양학회 1997 한국운동영양학회 학술발표논문집 Vol.- No.2

학술발표 : 현대인의 건강증진방안 : MSG 로 유도한 비만 쥐의 구축

장응찬(Eung Chan Jang),권오선(Oh Sun Kwon),이수천(Soo Chun Lee) 한국운동영양학회 2001 한국운동영양학회 학술발표논문집 Vol.- No.9

Intervention of a Multi-channeled Oral Irrigation Device for Improving Oral Health in Rehabilitation Patients After Stroke

Chan Gyu Kim,Geun Su Park,Jong Hwa Jang,Se Eung Noh,Hyun Jeong Kim,Seong A Lee 국제차세대융합기술학회 2024 차세대융합기술학회논문지 Vol.8 No.3

본 연구의 목적은 재활병원에서 뇌졸중 환자의 구강관리를 위한 다채널구강세정기(MCOI)의 효과와 안전성 을 평가하는 것이다. 다채널구강세정기로 수집된 구강세척수의 탁도 및 음식물 잔사를 평가하였으며, 구강건강지표 인 설태와 혀마름을 함께 평가하였다. 인지장애를 동반한 11명의 환자를 포함하여 24명의 뇌졸중 환자는 부작용 없이 다채널구강세정기를 사용하였다. 연구 결과, 다채널구강세정기를 3회 연속으로 적용 후에 구강세척수의 탁도와 음식물 잔사가 유의하게 감소하였다(p<0.001). 결론적으로, 뇌졸중 환자에게 다채널구강세정기를 사용 후 구강청결 효과가 나타나 구강관리가 힘든 뇌졸중 환자의 새로운 대안이 될 수 있고, 일상적인 구강관리 뿐만 아니라 삼킴재 활치료를 하는 동안 뇌졸중 환자에게 구강청결을 시행하는 치료사의 시간을 절약할 수 있을 것으로 생각된다. The purpose of this study is to evaluate the efficacy and safety of a multi-channel oral irrigation (MCOI) device for oral care in stroke patients at a rehabilitation hospital. Turbidity and food residue in oral irrigated water collected by the MCOI were assessed, along with oral health indicators such as tongue coating and dryness. Twenty-four stroke patients, including 11 with cognitive impairment, used the MCOI without adverse effects. Following 3 consecutive cycles of the MCOI, there was a significant reduction in the turbidity and food residue of the mouth-rinsed water (p<0.001). In conclusion, the use of the MCOI in stroke patients resulted in improved oral hygiene, offering a new alternative for challenging oral care in stroke patients. Additionally, it may save therapists' time during swallowing rehabilitation sessions by incorporating oral cleaning practices.

흰쥐에서 트레드밀 운동이 인슐린 분비에 미치는 영향

장응찬,박덕일,이수천 한국운동영양학회 1997 Physical Activity and Nutrition (Phys Act Nutr) Vol.1 No.2

The purpose of this study was to investigate the effects of exercise upon insulin secretion through male Sprague-Dawlay rats. That is to say, it was to determine whether exercise reduces insulin secretion or not. The rats were randomly divided into seven exercise-trained rats(SG) and seven sedentary rats(SG), and EG was subjected to a six weeks physical training by means of treadmill running four days per week. The experiment on EG was carried out in the state of overnight fast forty-eight hours after the exercise in order to eliminate the effect of the last bout of the exercise. This study was successively performed by three stages to find out insulin secretion response to stimuli for 130 minutes. At first, arginine bolus was injected in basal level, and insulin secretion response to arginine stimulus was evaluated 40 minutes after the injection. Next, hyperglycemic clamp procedure was introduced for 20 minutes, and insulin secretion response to glucose stimulus was evaluated Finally, arginine was additionally infused in the state of a hyperglycemic clamp(stage 2), and insulin secretion response to synergism of both glucose and arginine stimuli was evaluated. The results were as follows. When arginine bolus was injected in basal level, plasma insulin concentration in EG was lower than that in SG. Secretory response of insulin in EG during hyperglycemic clamp was lower than that in SG. When arginine was additionally injected in the state of a hyperglycemic clamp, plasma insulin concentration in EG was lower than that in SG. From the above results, the reduced plasma insulin concentrations in response to these stimuli in EG could be explained either by a blunted sensitivity of the β-cell or by a reduced capacity of the β-cell tp secrete insulin in vivo.