위 유문동 평활근세포의 Caffeine 활성화 전류에 미치는 Ascorbate의 효과

이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4

In order to observe the effects of ascorbate on the background current in pyloric circular smooth muscle cells, caffeine of which action was fairly clarified, was used to induce the current systems. To block all of the known currents, various blocking agents were added in the external bathing solution. A part of pyloric circular muscle tissue was excised out, and enzymatic isolation technique was used to obtain Ca^2+ -tolerant single circular muscle cells. The whole cell mode of patch-clamp experiment was conducted. The results were as follows : 1. Ascorbate induced the increase of inward currents in the hyperpolarized potential ranges, and quite a large increase of outward currents in the depolarized ranges. 2. Caffeine also induced similar results, but the magnitude of outward current was smaller than that of assorbate in the depolarized ranges. 3. These phenomena were disappeared by addition of the anion channel blocker. 4. Replacement of external NaCl with NMG·Cl was rarely affected the effects of ascorbate and and caffeine. But the effects of ascorbate and caffeine were almost abolished when sxternal Cl^- was substituted for aspartate. These results suggested that ascorbate affected chloride current which thought as a component of the background currents in the pyloric circular smooth muscle cells.

광고 모델의 속성을 이용한 기업광고가 기업 이미지에 미치는 영향

이호배,김대열 홍익대학교 경영연구소 1995 경영연구 Vol.20 No.-

p-n 접합을 이용한 ISFET의 전기적 절연

이강열,강명성,김창수,서화일,권대혁,손병기 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

제철소폐기물로부터 Calcium Ferrite의 제조

이대열,정원배 釜山大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.52 No.-

최근 환경규제가 강화되면서 주로 매립에만 의존하던 제철공정에서 발생하는 분진 및 슬러지 등의 폐기물을 재활용하는 연구가 진행되고 있다. 소결공정에서 발생하는 전기집진 분진(Fe₂O₃원)과 석회석 수세시 발생하는 슬러지(CaO,원)를 7:3 비율로 혼합한 후 1150∼1200℃에서 소성하여 철광석 소결광의 결합물질인 Calcium ferrite를 인위적으로 제조하였으며 이를 소결원료중 석회석과 대체하여 소결모사 시험을를 실시한 결과 소결생산성 및 소결광의 강도가 향상되었다. Recently, anti-pollution technologies have become a necessity and are required to protect the environment of the air, ground and water. A total recycling system was studied in order to produce the synthetic calcium ferrite (SCF) from wastes including a sinter waste E.P. (Electrostatic Precipitator) dust and limestone sludge, to be used as the flux in the sintering process. SCF could be made from a sinter waste E.P. dust and limestone sludge in a mixing ratio of 7:3 at a sintering temperature of 1150∼1200℃. In sinter simulation pot tests, the productivity and strength of the sinter showed a tendency to improve as the substitution ratio of the SCF increases from 0 to 50%.

자궁경부에 발생한 배아성 횡문근육종 2예

박정열,조준식,김대연,이동헌,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.3

횡문근육종은 소아와 청소년기에 가장 흔한 연부조직 육종이다. 발생 부위는 일반적으로 두부 및 경부, 그 다음으로 비뇨생식계이다 조직학적으로 배아성 횡문근육종, 국화상 육종, 폐포성 횡문근육종, 다형성 횡문근육종으로 나누어진다. 횡문근육종의 약 20%가 비뇨생식기에서 발생하고, 50% 이상이 배아성 횡문근육종이다. 여성 생식기의 배아성 횡문근육종은 드문 악성 종양으로,주로 유소아의 질에서 발생하며, 자궁경부에서 발생하는 횡문근육종은 청소년기에 흔하고, 질에서 기원하는 횡문근육종이 자궁경부에 기원하는 경우보다 5배 정도 많다. 폐경기여성에서는 자궁체부에서 가장 흔히 발생한다. 비뇨생식기의 횡문근육종의 치료는 과거에는 골반장기 적출술만으로 치료를 시도하였으나. 서서히 다중 항암화학요법, 방사선요법, 근치적 절제술을 포함한 병합요법으로 바뀌어가고 있으며, 이에 따라 생존율이 현저하게 향상되었다. 이에 저자들은 최근 본원 산부인과에서 자궁경부에 발생한 배아성 횡문근육종 2예를 경험하였기에 문헌 고찰과 아울러 보고하는 바이다. Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood and young adult. Genitourinary tract is the econd most common site of rhabdomyosarcoma. Rhabdomyosarcoma can be classified into one of four major types; embryonal, alveolar, pleomorphic and undifferentiated. Embryonal rhabdomyosarcoma of the female genital tract is rare malignant tumor and usually occurs during childhood in the vagina. In rare cases, rhabdomyosarcoma can originate in the uterine cervix, with a peak incidence in the second decade. Recently we have experienced two cases of embryonal rhabdomyosarcoma of the uterine cervix. These cases are presented with a brief review of the literature.

一柱門의 建築形式에 關한 硏究

정대열,이재환,이용대,예명해 대구대학교 과학기술연구소 2001 科學技術硏究 Vol.8 No.2

The purpose of this study is to discover the architectural type of 「Il-joo-moon」 It deals with 「Il-joo-moon」 that has been built before 1910 in Gyoung-sang province. This study analyzes type of member, jointing method, frame structure method by dividing 「Il-joo-moon」 into four parts such as roof, bracket system, frame structure, podium. The results of this analysis are as follows 1) Roof: The 「Pal-ssac」 style of roof and the 「Matt-bae」 style of roof are dominant and double caves mainly appears. 2) Bracket system: The arrangement of bracket system can be divided into one-line-arrangement and two-line-arrangement. As far as 「Chul-mock-soo」 concerned, 3 or 4 「Chul-mock-soo」 is predominent. 「Tong-jae-gong」 has been used to connect front and back bracket system for some 「Il joo-moon」. When it comes 「jae-gong」, 「Sue-soe」 style, 「Yon-bong」 style and 「Yon-hwa」 style are mainly seen. 3) Frame structure: There are 5 forms in classifying frame structure depending on the jointing method of main-column, diagonal member, sub-column etc. and weight transmission method. 4) Podim: Natural foundation stone is majority. It is judged that 「Il-joo-moon」 has no podium or natural ones, however, it has recently been converted into artificially processed podium in many cases.

인간 타액선세포 및 선암종세포주의 분화 유도시 Transglutaminase발현과 고정의존성 성장과의 관계

박대열,이선경,이화정,장현주,민승기,김은철 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

Transglutaminase(TGase) catalyzes the Ca^2+ dependent acyl transfer reaction to from γ-glutamyl lysine cross-links between substrate proteins. we have examined the expression of TGase 1 and TGase 2 enzyme activity and protein expression in differentiation induced salivary gland cells(HSG), and human salivary adenocarcinoma cell lines(SGT)in reation to anchorage independent growth. The TGase1 and TGase 2 activity in SGT was higher than in HSG, and there is no significant difference between TGase 2 and TGase1 cells. HSG cells showed no increase in TGase 1 and TGase 2 upon reaching post confluence and in 1.8mM Ca^2+. Expression of the TGase 1 and TGase 2 was induced by high cell density or by high calcium in SGT. No induction of TGase 1 & 2 protein was observed in postconfiuent HSG cells, but there was notable increases in TGase 1 & 2 protein in post confiuent SGT cells. This pattern of TGase 1 & 2 protein expression correlates with the pattern of particulate activity. SGT cells showed more colony size and colony forming activity in soft agar rather than HSG cells. The ability of SGT cells, containing high levels of TGase 2 and TGase1,to form colonies in soft agar suggests a role for this enzyme in anchorage independence.

실업계 고등학생의 직업기초능력 향상을 위한 문제중심학습(PBL)의 효과

박동열,이성덕,김종오,조경희,강경심,박윤희,김대영 한국농업교육학회 2006 농업교육과 인적자원개발 Vol.38 No.4

21세기 지식기반사회는 전문지식뿐만 아니라 효율적인 직무 수행에 필요한 의사소통능력, 문제해결능력, 자기관리능력 및 직업의식 등의 직업기초능력이 강조되고 있다. 직업기초능력은 생의 어느 한 시기에 갑자기 이루어지거나 완성되는 l회적인 것이 아니라 개인의 생애단계에 따라 변화하고 발달하는 것이며 취업자뿐만 아니라 진학자에게도 잠재적으로 매우 중요한 요소이므로 실업계 고등학생의 직업기초능력 향상은 중요한 현안이라 할 수 있다. 이 연구는 여러 교수학습유형 중 실업계 고등학생의 직업기초능력 향상에 타당하리라 사료되는 문제 중심학습 모형(Problem-based learning model)을 선정, 이에 기초한 전문교과 수업을 실시하여 실업계 고등학생의 직업기초능력 향상에 기여할 수 있는지 실증적으로 분석하고자 하였다. 연구 목적 달성을 위해 「대학생의 직업기초능력 유형 검사도구(박동열, 2005)」를 실업계 고등학교 전문가 협의회를 통해 수정ㆍ보완, 이를 활용하여 실업계 고등학생의 직업기초능력을 파악하고, 문제중심학습 모형에 따른 실제 전문교과 수업을 통한 실업계 고등학생의 직업기초능력 향상 정도를 알아보기 위하여 농업계열, 공업계열, 상업계열, 가사ㆍ실업계열 4명의 현직 교사가 문제중심학습 수업 매뉴얼에 따라 실제 수업에 적용 하고 그 결과를 분석하였다. 연구 결과 문제중심학습 모형이 실업계 고등학생의 수업만족도에 긍정적인 영향을 끼쳤으며, 문제중심학습 모형에 따른 전문교과 수업을 통해 실업계 고등학생의 모든 영역의 직업기초능력(문제해결 및 발표 능력, 기술활용능력, 자원활용능력, 수리능력, 조직이해능력, 대인관계능력, 자기관리능력)이 향상되었음을 실증적으로 알 수 있었다. The purpose of this research was to analyze the fitness of problem-based learning(PBL) for improving the core competencies of vocational high school students. This study examined the core competencies of vocational high school students by using the advised BARS test for vocational high school. And in order to examine the effectiveness of the courses based on PBL, 4 teachers had taught the designed courses by PBL method during the regular class in vocational high school : agricultural; technical; commercial, home economic high schools. The major findings in this study were as follows: 1) The courses based on PBL had an positive effect on the class satisfaction of vocational High school students. 2) We found out that the courses based on PBL have a positive effect on improving vocational key competencies of Vocational High school students.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

위 윤상근 세포의 Ca^2+-의존성 K^+-전류에 미치는 Ascorbate의 효과

배정원,이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2

In order to observe the effect of ascorbate on the Ca^2+-activated K^+ current in pyloric circular muscle cells which plays a critical role in generation and maintenance of repolarization and automatism, apart of pyloric circular muscle tissue was excised out, digested in collagenase containing Ca^2+-free physiological solution to get single circular muscle cells and the whole cell mode of patch-clamp experiment was conducted. Following results were obtained : 1. Oscillatory outward current was reduced by ascorbate in case of high intrapipette Ca^2+ concentration in whole voltage range and was enhanced in case of low intrapipette Ca^2+ concentration. 2. Oscillatory outward current was reduced in Ca^2+-free physiological solution with calcium channel blocker and not effected by ascorbate. 3. There were no qualitative differences among the control and ascorbate-treated group in effects of pH, steady state inactivation and the remaining component of the outward current. The above results could be concluded that ascorbate effects calcium-dependent component of the oscillatory outward current in the pyloric circular smooth muscle cells.