저온처리한 오이의 자엽에서 광합성 활성의 광의존성 저해

鄭翼敎,李春煥,朴仁虎,全鉉植,文炳鎔,李鎭範 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

저온에 노출된(4℃에서 24시간)오이 유식물에서 자엽의 산소 발생률 및 quantum yield, CO₂고정능, 실온에서 측정한 엽록소 형광유도의 변화 등을 조사하였다. 저온의 광조건에 노출된 오이 유식물에서 자엽의 최대 산소 발생률, quantum yield, CO₂고정능, variable fluorescence가 현저히 감소하였다. 특히 저온의 광조건은 엽록소 형광유도에서 Fo를 증가시키고 (Fv)m/Fm을 감소시켰는데 이는 광저해로 인하여 광계 II의 반응중심이 손상되고 광합성 활성이 감소된데서 기인한 것으로 생각된다. 또한 저온처리 동안 엽록소 형광소멸에서도 암조건보다 광조건에서 qQ가 크게 감소하였으나 qNP는 차이가 없었고, 저온처리 이후의 회복과정에서도(24시간) 암조건보다 광조건에서 엽록소 형광의 relaxation이 느리고 낮게 나타나 오이 잎의 광합성 기구가 저온의 광처리에 의해 보다 심하게 저해되었음을 알 수 있었다. 특히 stress indicator인 ??은 저온의 암조건에서 20% 감소한 반면, 광조건에서는 60%나 감소하여 오이의 광합성기구가 저온에 매우 민감하게 반응하는 것 으로 생각되었으며 이는 광합성기구에 대한 저온장해가 뚜렷하게 광의존성임을 나타낸다. The photosynthetic activities in relation to oxygen evolution rates, quantum yield, CO₂ uptake rates and room temperature chlorophyll fluorescence were investigated in cotyledons of cucumber seedlings exposed to low temperature(at 4°C) for 24 h. Light-chilling caused more inhibition on light-saturated maximum oxygen evolution rates, quantum yield, and CO₂uptake rates than dark-chilling did in the cucumber plant. Light-chilling induced more marked increase in Fo and decrease in (Fv)m/Fm than dark-chilling did in the room temperature chlorophyll induction kinetics. The above results affected by chilling in the light are considered to be associated with the partial damage of the reaction center of PS Ⅱ and the decreased photosynthetic activities. There occurred a large decrease in qQ with little change in qNP in the light-chilling plant. When light-and dark-chilled plants were recovered at room temperature for 24 h and their chlorophyll fluorescences were induced with light doubling technique, light-chilled plants showed more smaller magnitude and rate of fluorescence relaxation than dark-chilled plants. These suggest that light-chilling might cause some alterations in transthylakoid △pH formation, and that photosynthetic apparatus of cucumber cotyledons is more susceptible to light-chilling In the fast fluorescence induction kinetics ?? was decreased by 60% in the light-chilled plants with reference to 25°C light-grown plants, while the dark-chilled plants showed a decreased rate of only 20% with reference to 25°C dark-treated plants for 24h, indicating that cucumber seedling is very sensitive to chilling stress. So, it is certain that chilling injury to the photosynthetic apparatus is strongly dependent on the presence of light in cucumber seedlings.

보리 유식물에 처리한 수은의 분포 및 독성 연구

이춘환,이진범,장호식,문병용,정익교,전현식,이진애 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et al.(1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of 10μM mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attached, but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.

Prospidin이 Mouse의 간장에 미치는 영향

이규식,정호삼,이민형,백두진,기춘석 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

Prospidin, a new alkylating agent, has antimitotic and anticancer effect. Incubation with prospidin increases the stability of DNA and suppresses the incorporation of uridine into RNA by 15-20 percent. Prospidin is cytostatic for suspension culture and cells are blocked in G₂ phase of the cell cycle. The authors have demonstrated the effect of prospidin on the liver of mice histologically and histochemically and observed the cellular changes, loss of pyroninophilic granules and accumulation of fat in the hepatic parenchymal cells. Sixty-five healthy mice of ICR strain, average weight being 20g, were divided into two groups; control and prospidin-treated groups. All experimental animals were administered foo and water ad libitum. Animals of the prospidin-treated groups were administered prospidine 500mg per kg of body weight intraperitoneally fter dilution in the water for injection. Animals of the control group were administered only water for injection. Prospidin treated animals were sacrificed at intervals of 6, 12, 24, 36 and 48 hours after administration of prospidin. Histological preparations were made by way of hematoxylin and eosin stain for the cellular changes, methyl green-pyronin stain for pyroninophilic granules and oil red O stain for fat accumulation in the mouse liver. The results obtained are as follows. 1. Cellular swelling associated with vesicular formation were observed in the hepatic parenchymal cells of entire hepatic lobule at 24 and 36 hours after administration of prospidin. 2. A marked reduction of the pyroninophilic granules were observed in the hepatocyte of he whole hepatic lobule at 24, 36 and 48 hours following drug administration. 3. Fat deposits in the hepatic lobule were seen at 36 hours after administration of prospidin. It is suggested that prospidin acts as a cytotoxic agent and causes cellular swelling with vesicular formation, loss of pyroninophilic granules, and fat accumulation in the parenchymal cells of the liver.

저온처리한 오이의 자엽에서 광합성 활성의 광의존성 저해

全鉉植,文炳鎔,李春煥,鄭翼敎,朴仁虎,李鎭範 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

The photosynthetic activities in relation to oxygen evolution rates, quantum yield, CO₂uptake rates and room temperature chlorophyll fluorescence was investigated in cotyledons of cucumber seedlings exposed to low temperature(at 4℃) for 24 h. Light-chilling caused more inhibition on light-saturated maximum oxygen evolution rates, quantum yield, and CO₂uptake rates than dark-chilling did in the cucumber plant. Light-chilling induced more marked increase in Fo and decrease in (Fv)m/Fm than dark-chilling did in the room temperature chlorophyll induction kinetics. The above results affected by chilling in the light are considered to be associated with the partial damage of the reaction center of PS Ⅱ and the decreased photosynthetic activities. There occurred a large decrease in qQ with little change in qNP in the light-chilling plant. When light- and dark-chilled plants were recovered at room temperature for 24 h and their chlorophyll fluorescences were induced with light doubling technique, light-chilled plants showed more smaller magnitude and rate of fluorescence relaxation than dark-chilled plants. These suggest that light-chilling might cause some alterations in transthylakoid △pH formation, and that photosynthetic apparatus of cucumber cotyledons in more susceptible to light-chilling. In the fast fluorescence induction kinetics, F_R was decreased by 60% in the light-chilled plants with reference to 25℃ light-grown plants, while the dark-chilled plants showed a decreased rate of only 20% with reference to 25℃ dark-treated plants for 24h, indicating that cucumber seedling is very sensitive to chilling stress. So, it is certain that chilling injury to the photosynthetic apparatus is strongly dependent on the presence of light in cucumber seedlings.

조직구성 수성망상증 2예

최연수,박일,죄희충,김원식,양태영,허경,안기완,정춘해 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

Histiocytic medullary reticulosis (HMR) is a malignant noeplastic process, with tumor cells belonging to the histiomonocytic line, and originally described by Scott and Robb-Smith in 1939 characterized by its acute onset, progression to death within a few months and its clinical features are fever, lymphadenopathy, hepatosplenomegaly, jaundice, and purpura common laboratory findings are characterized by severe anemia, leukopenia, thrombocytopenia and histologically by systemic progressive invasion of morphologically atypical histiocytes or of their precursor. 61 and 37-year-old males were admitted with camplaints of high fever and chilling sensation and were treated by cyclophosphamide, vincristine, prednison and antiobiotics, but patients all died 17 and 14 days after admission due to probably sepsis and respiratory failure.

원발성 폐암 환자의 생존율에 관한 보고

김현태,이상무,어수택,박춘식,정성환,허승재,남충희,강창희,김용훈 순천향대학교 1994 논문집 Vol.17 No.4

We analysed 404 patients with primary lung carcinoma who were treated at Soonchunhyang University Hospital from July, 1985 to september, 1993 in order to investigate the survival rate and epidemiolgical properties of primary lung cancer. They were 330 males and 74 females. The most prevalent decade was seventh. In terms of cell type, the squamous cell was 225 patients (55%), and adenocarcinoma, small cell, mixed type was 21%, 19%, 4%, respectively. Among non-small cell lung carcinoma, stage Ⅲa was the most prevalent one(92%). In case of small cell carcinoma, the limited stage was 64%. The 12-, 24-, 36- month survival rate of total patients was 57%, 31%, 22%, respectivley and median sruvival time was 15 months. The 36-month survival rate tended to be longer in non-small cell lung carcinoma than that of small cell lung cancer, but there was no difference between two groups, statistically. In non-small cell carcinoma, The 36-month survival rate and meidan survival time were longer in the stage Ⅰ and Ⅱ than those of Ⅲa, Ⅲb, Ⅳ (80% versus 38%, 22%, 0%, p<0.05). According to involvement of lymph node, the 36-month survival rate was longer in NO and N1 than those of N2, N3 (61.9%, 48.7% versus 17.7%, 17.3%, p<0.05). In small cell carcinoma, The 36-month survival rate and median survival rate were higher and longer in limited stage than those of extensive stage(16.1% and 13 month vs 10% and 8 month, p<0.05). In conclusion, we report here the incidence of primary lung carcinoma and the survival rate of paients with primary lung carcinoma who were treated in Soonchunhyang University Hospital.

Inguinal hernia developed after radical retropubic surgery for prostate cancer

Choon Sik Chung,Gyu Young Jeong,Seung Han Kim,Dong Keun Lee 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.85 No.4

Purpose: In this retrospective study, we aimed to compare the clinical characteristics of inguinal hernia developed after radical retropubic surgery for prostate cancer to the hernia without previous radical prostatectomy. Methods: Twenty-three patients (group A) who had radical retropubic surgery for prostate cancer underwent laparoscopic or open tension-free inguinal hernia repair from March 2007 to February 2011. Nine hundred and forty patients (group B) without previous radical retropubic surgery received laparoscopic or tension-free open hernia operation. Results: Group A was older than group B (mean ± standard deviation, 69.6 ± 7.2 vs. 54.1 ± 16.1; P < 0.001). Right side (73.9%) and indirect type (91.3%) in group A were more prevalent than in group B (51.5% and 69.4%, respectively) with statistic significance (P = 0.020 and P = 0.023). The rate of laparoscopic surgery in group B (n =862, 91.7%) was higher than in group A (n = 14, 64.3%, P < 0.001). In comparing perioperative variables between the two groups, operative time (49.4 ± 23.5 minutes) and hospital stay (1.9 ± 0.7 days) in group A were longer than in group B (38.9 ± 16.9, 1.1 ± 0.2; P = 0.046 and P < 0.001, respectively) and pain score at 7 days in group A was higher than in group B (3.1 ± 0.7 vs. 2.3 ± 1.0, P < 0.001). Postoperative recurrence rate was not significantly different between the two groups. Conclusion: Inguinal hernia following radical retropubic surgery for prostate cancer was predominantly right side and indirect type with statistic significance compared to hernias without previous radical prostatectomy.

만성적인 후두증상을 주소로한 환자에서의 식도내압검사 및 24 시간 보행성 pH 검사소견

방춘상(Choon Sang Bhang),노대근(Dae Keun Lo),박수헌(Soo Heon Park),최명규(Myung Gyu Choi),한준열(Jun Yeul Han),정인식(In Sik Chung),정규원(Kyu Won Chung),선희식(Hee Sik Sun),박두호(Doo Ho Park),김부성(Boo Sung Kim),조승호(Seung Ho Ch 대한내과학회 1996 대한내과학회지 Vol.51 No.6

N/A Objectives: The exact causes of nonspecific chronic laryngitis or laryngeal symptoms are unknown, but many cases of this disease are associated with gastroesophageal reflux or motility disorders of esophagus. We suppose that because Korean people have less gastroesaphageal reflux diseases and the life style including food is different from those of western people, the causes of chronic laryngeal symptoms nre different, So we studied the patients who complained the chronic laryngeal symptoms for evaluation of the role of esophageal motility disorders and gastroesophageal reflux. Methods: 19 patients(male 3, female 16, mean age 40(26-66)) with hoarseness, globus sensation, sorethroat of chronic nature(greater than 3-month duration), not response to conventional therapy of otolaryngologist in St. Mary's Hospital were referred to department of internal medicine. A group of normal, 15 healthy subjects(male 7, female 8, mean age39(24-57)) who had no esophageal or laryngeal symptoms was studied as a control group. All patients and normal controls had manometric studies and 24 hour ambulatory pH monitoring. abnormal findings required pathologic reflux or results greater than the mean plus 2 SDs of normal controls. Results: 1) The chronic laryngeal symptoms were hoarseness in 15 patients(79%), globus sensation in 14 patients(74%), sorethroat in 6 patients(32%), pulmonary symptoms in 3 patients(16%), dysphagia in 2 patients(11%) and heartburn in 1 patient(5%). 2) The lower esophageal sphincter pressure by the rapid pull through tecnique(mean±SD, 27,9± 6.9mmHg) in patients was not different compared with normal controls(31,1±11.0nmHg), 3 patients (16%) had esophageal body dysmotility and all of these were non specific motility disorder. 3) The upper esophageal sphincter pressure(mean±SD, 53.2±23.8mmHg) in patients was not different compared with normal controls(40.9±20.8mmHg), but 2 patients(10.5%) had high upper esophageal sphincter pressures(>82,5mmHg), The postrelaxation pressure of upper esophageal sphincter(mean±SD,98.3±32.1mmHg) was not different compared with normal controls(79,8±30.6mmHg), but 2 patients(10.5%) had high postrelaxation pressures of upper esophageal sphincter(>141mmHg). 4) The time(mean±SD, 321±193msec) between lowest point of upper esophageal sphincter relaxation and onset of pharyngeal contraction was not different compared with normal controls(273.5±179,8msec). 5) The percentage of time of pH less than 4 was 2.4±4.7% in total periods, 2.5±2.3% in upright position and 2.4±9.7% in supine position. Two patients (10.5%) had pathologic reflux(more than 4% of percentage of time pH less than 4 in total periods) and one patient with 2.5% of persentage of time pH less than 4 had high symptom index(66.7%(4/6)). 6) According to above results either of abnormal esophageal pressure or dysmotility was seen in 6 patients(32%) and either of more than 4% of persentage time pH less than 4 in total periods or positive symptom index was seen in 3 patients(16%), Positive results in either of two tests were seen in 7 patients(37%). Conclusion We suggest that some patients who complain the chronic laryngeal symptoms in Korean may have abnormal esophageal motility or gastroesophageal reflux.

CHANGES OF ELECTROGASTROGRAM IN MAINTENANCE HEMODIALYSIS PATIENTS - DOES HYPOVOLEMIA CAUSE GASTRIC DYSRHYTHMIA ?

( In Sik Chung ),( Hyun Suk Choi ),( Soo Heon Park ),( Myung Gyu Choi ),( Hee Sik Sun ),( Doo Ho Park ),( Kyu Won Chung ),( Choon Sang Bhang ) 대한소화기학회 1995 대한소화기학회 추계학술대회 Vol.1995 No.-

신생아에 대한 가열 비 활동화한 B형 간염백신의 안전성과 면역성에 대한 임상적 관찰

전정식,이익준,심규식,정승연,정진우,최규용,조규홍,강진환,김승조,선희식,성인경,정규원,정환국,김부성 대한감염학회 1987 감염 Vol.19 No.4

A new heat-inactivated HBV vaccine has been evaluated for safety and immunogenicity in 157 newborn recipients. Three(Group 3M), one and a half(Group 1.5M), and one(Group 1M) μgm(HBsAgprotein) doses were given at 0,1 and 2 months.Three(Group 3W) and(Group 1W) μgm doses were also given at 0,1 and 2 weeks. All vaccine was administered intramuscularly in the thigh muscle. An adequate follow-up observation was possible during the period of 6 months after birth in 33, 26, 28, 34 and 36 infants in Groups 3M, 1.5M, 1M, 3W and 1W, respectively. Side effects were minor with limited local reaction at the site of adminisration. The 3W group produced seroconversion earliest and most rapidly, showing the highest rate of 60% and 70% at 1 and 2 months of age, resectively. However, at the age of 3 months, the highest seroconversion rate of 91.7% was observed in Group 1.5M, followed by 84.6% in Group 3W, 66.7% in Group 3M, 61.5% in Group 1M and 57.1% in Group 1W. At the age of 6 months, 87.5% of Group 3W, 80.8% of Group 1M, 80.4% of Group 1.5M, 83.3% of Group 3M infants showed seroconversion. However, a drop to 44.4% seroconversion was seen in Group 1W. There was no significant differences in seroconversion rates between Groups 3W, 1M, 1.5M and 3M at 6 months of age. There was also very little difference between these 4 Group regarding to the value of antibody response in terms of geometric mean titer. The vaccine was found to be safe and highly immunogenic for newborn infants. It can be concluded that 1.5M schedule is optimum for the immunization of newborn infants in immunogenicity and economy, while the 3W schedule can induce the earliest and most rapid seroconversion.