藏傅佛教在台發展

정지명 강남대학교 인문과학연구소 2004 인문과학논집 Vol.13 No.-

Angelica sinensis Supercritical Fluid CO2 Extract Attenuates D-Galactose-Induced Liver and Kidney Impairment in Mice by Suppressing Oxidative Stress and Inflammation

Zhi-Zhun Mo,Zhi-Xiu Lin,ZiRen Su,Lin Zheng,Hui-Lin Li,JianHui Xie,Yan-Fang Xian,Tie-Gang Yi,Shui-Qing Huang,Jian-Ping Chen 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9

Angelica sinensis (AS, Danggui in Chinese) is an important herbal component of various traditional formulae for the management of asthenia and its tonic effects. Although AS has been shown to ameliorate cognitive damage and nerve toxicity in D-galactose (D-gal)-elicited senescent mice brain, its effects on liver and kidney injury have not yet been explored. In this work, mice were subjected to hypodermic injection with D-gal (200 mg/kg) and orally gavaged with AS (20, 40, or 80 mg/kg) once a day for 8 successive weeks. Results revealed that AS significantly improved liver and kidney function as assessed by organ index and functional parameters. In addition, AS pretreatment effectively ameliorated the histological deterioration. AS attenuated the MDA level and markedly enhanced the activities and gene expressions of antioxidative enzymes, namely Cu, Zn-SOD, CAT, and GPx. Furthermore, AS markedly inhibited the D-gal-mediated increment of expressions of inflammatory cytokines iNOS, COX-2, IκBα, p-IκBα, and p65 and promoted the IκBα expression level in both hepatic and renal tissues. In sum, AS pretreatment could effectively guard the liver and kidney of mice from D-gal-induced injury, and the underlying mechanism was deemed to be intimately related to attenuating oxidative response and inflammatory stress.

Determination of 2-(3-benzoyl)-4-hydroxy-1,1-dioxo-2H-1,2-benzothiazine-2-yl-1-phenylethanone by liquid chromatography-tandem mass spectrometry.

Zheng, Zhi,Song, Jin Sook,Lee, Byung Hoi,Ahn, Sung-Hoon,Ahn, Jin-Hee,Woo, JaeChun,Park, Ji-Young,Yoo, Dae Seok,Bae, Myung Ae 3M Company 2014 Journal of Chromatographic Science Vol.52 No.5

<P>2-(3-Benzoyl)-4-hydroxy-1,1-dioxo-2H-1,2-benzothiazine-2-yl-1-phenylethanone (KR-66344), a 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) inhibitor, is newly developed for the control of type 2 diabetes mellitus (T2DM) and metabolic syndrome. A method for the determination of KR-66344 in rat plasma was developed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) to evaluate the pharmacokinetics of KR-66344. Plasma samples were processed by a liquid-liquid extraction method with ethyl acetate and introduced onto the LC-MS-MS system. The analyte and imipramine (internal standard) were analyzed by multiple reaction monitoring based on transitions at m/z 420.1 105.0 and 282.2 86.0, respectively. The calibration curve was linear (r = 0.9993) over the concentration range of 1.0-1,000 ng/mL. The mean recovery values for KR-66344 and imipramine were 83.8 and 86.2%, respectively. The mean inter-day and intra-day assay precision values were 3.9 and 2.4%, respectively. KR-66344 was stable under various handling and storage conditions. This developed method was applied to a pharmacokinetic study after the oral administration of KR-66344 in rats. The concentration of KR-66344 was readily measurable in rat plasma up to 24 h post-dose after an oral administration, suggesting that current assay is applicable to pharmacokinetic studies for KR-66344.</P>

Beneficial Effects of Fermentation of Red Chili Pepper Using Lactococcus lactis subs. Cremoris RPG-HL-0136 in High-Fat Diet-Induced Obese Mice

Zhi Zheng,Jung Kuk Park,Linjuan Jiang,Shaohui Zhu,권오욱,이병천,이혜민,노연진,강재현,박병희 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.2

Red chili pepper is a beneficial natural spicy food that has antiobesity and antitype II diabetes effects, but it is not conducive to in-depth research as a dietary strategy to treat obesity. This study aims to investigate the beneficial effects of red chili pepper, fermented with a novel Lactococcus lactis subs. cremoris RPG-HL-0136. LC-MS/MS analysis is conducted to detect the content of capsaicin and dihydrocapsaicin, and no significant difference is observed between the nonfermented red chili pepper (NFP) (W/W) and the prepared L. lactis subs. cremoris RPG-HL-0136-fermented chili mixture (LFP). After establishing a high-fat diet-induced obese type II diabetic mouse model, the effects on weight gain, weight loss of liver and testicular fat, total cholesterol, triglyceride, fasting glucose, insulin, and homeostatic model assessment for insulin resistance in LFP were evaluated to be better than those in NFP following 10 weeks of interventions. All animal experiments were approved by the Institutional Animal Care and Use Committee of Xinxiang medical university. NFP and LFP could increase the expression of transient receptor potential vanilloid subfamily 1, peroxisome proliferator-activated receptor-alpha and caspase-2 in the high-fat mice. Compared with unfermented red chili pepper, the fermented red chili pepper complex significantly reduced LPS, tumor necrosis factor-alpha, and interleukin-6 in serum (P < .05). Intake of LFP significantly increased the expression of claudin-1 and occludin in the colon of the high-fat mice (P < .05), and there was no damage to the stomach and colon. This study provides scientific evidence that red chili pepper, fermented with L. lactis subs. cremoris RPG-HL-0136, may be beneficial for future treatment of obesity and accompanying diabetes. (IACUC.No.XYLL-20200019).

Historical Long-term Exposure to Pentachlorophenol Causing Risk of Cancer - A Community Study

Zheng, Rui-Zhi,Zhang, Qing-He,He, Yi-Xin,Zhang, Qian,Yang, Lin-Shen,Zhang, Zhi-Hua,Zhang, Xiu-Jun,Hu, Jing-Ting,Huang, Fen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: Pervious studies suggested occupational workers exposure to pentachlorophenol (PCP) might contribute to increased risk of cancer. However, few studies have focused on associations between PCP and cancer risk at the community level. Objective: The present study was to explore the cancer risk for the community population living long-term in a PCP contaminated area. Methods: All the cancer cases diagnosed in 2009-2011 in Tongling City were collected. The cancer patients' residencies were geo-referenced in each district. The historical PCP usage for each district of Tongling was calculated as the PCP pollution index, which was further used to divide into PCP exposure categories. Standardized rate ratios (SRRs) of cancer incidence were applied to detect the cancer risk as exposure grade elevated. Correlation analysis was performed to analyze the relationship between PCP pollution and cancer incidence. Results: A total of 5,288 cancer cases (3,451 male and 1,837 female) were identified. PCP usage was correlated with the incidence of leukemia (r=0.88, P=0.002) for males, and with cancer of the esophagus for males (r=0.83, P=0.008) and females (r=0.71, P=0.020). Compared with the low exposure category, significant SRRs for total cancer sites was obtained for high PCP exposure category (SRR=1.61, 95%CI=1.59-1.62). Most SRR values of the cancer sites were significantly increased as exposure grade elevated and exposure time extended. Conclusion: The present study found that community residents living in the PCP contaminated area had increased risk of cancers. Leukemias, lymphomas and nasopharyngeal and esophageal cancers are most possibly associated with PCP exposure.

Seismic Fragility of a Typical Containment under Bidirectional Earthquake Excitations

Zhi Zheng,Xiaolan Pan,Xu Bao 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.11

Existing research to predict seismic fragility of Reinforced Concrete Containment (RCC) buildings does not take into account the impact of its inelastic bidirectional interaction arising from bidirectional horizontal ground motions. Hence, this study attempts to present a reliable and feasible method for fragility of the RCC subject to bidirectional ground motions. Three bidirectional load histories due to inelastic bidirectional interaction are firstly introduced to determine displacement-based failure limits for this structure. Nine bidirectional earthquake Intensity Measures (IMs) are developed to perform the bidirectional Incremental Dynamic Analysis (IDA). For the selection of an optimum method for fragility under bidirectional earthquake excitations, three fragility analysis methods are conducted and their results are compared. Results show that the maximum likelihood method yields better fragility results than the other two methods. The safety factor method for fragility cannot get the aleatory randomness properly. Besides, the first-mode geometric mean spectral acceleration SaGM(T1) is found to strongly correlate with the demand measure for the RCC and is suggested for deriving fragility curves for such structures under bidirectional earthquake excitations.

The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days

Zheng Zhi,Park Jung Kuk,Kwon Oh Wook,Ahn Sung Hoon,Kwon Young Joo,Jiang Linjuan,Zhu Shaohui,Park Byoung Hee 대한의학회 2022 Journal of Korean medical science Vol.37 No.30

Background: Benzo(a)pyrene (BaP) is a carcinogenic compound in contaminated foodstuffs. The effect of oral intake of the environmental carcinogen BaP under low doses and frequent exposure on a digestive system has not been thoroughly verified. Methods: In this regard, this study was conducted to prove the toxicity effects of BaP on the stomach and colon tissue after exposure to C57BL/6 mouse (3 and 6 µg/kg) following daily oral administration for 60 days. This study investigated acute gastric mucosal injury, severe gastric edema, cell infiltration, and mononuclear cells, multifocal cells, and tumoral inflammatory cells. Results: The results of ELISA showed that the expression of serum interleukin (IL)-6 and tumor necrosis factor-α in the BaP exposure group were significantly increased, and a high level of DNA adduct distribution in their stomach and colon. Moreover, this study has confirmed the expression of early carcinogenesis markers: nuclear factor (NF)-κB, p53, IL-6, superoxide dismutase 1 (SOD1), mucin (MUC1 and MUC2), and β-catenin in the stomach and colon, and showed that there was a significant increase in IL-6, NF-κB, SOD1, β-catenin, and MUC1 (P < 0.05). At the same time, there was a significant decrease in MUC2 and p53 (P < 0.05). Thus, even in low doses, oral intake of BaP can induce DNA damage, increasing the potential risk of gastrointestinal cancer. Conclusion: This study will provide a scientific basis for researching environmental contaminated food and intestinal health following daily oral administration of BaP.

Identification and characterization of BGL11(t), a novel gene regulating leaf-color mutation in rice (Oryza sativa L.)

Zhi-kun Wang,Yun-xiang Huang,Zheng-diao Miao,Zhi-yan Hu,Xin-zhang Song,Li Liu 한국유전학회 2013 Genes & Genomics Vol.35 No.4

A novel bright-green leaf mutant, bgl11, derived from Nipponbare (Oryza sativa L. ssp. japonica) treated by ethyl methanesulfonate (EMS), exhibited a distinct brightgreen leaf phenotype throughout development. Chlorophyll contents of bgl11 decreased significantly than that of its wild-type parent. Genetic analysis suggested that the brightgreen leaf trait was controlled by a single recessive nuclear gene, which was tentatively designed as BGL11(t). To isolate the BGL11(t) gene, a map-based cloning strategy was employed, and the gene was finally mapped in a 94.7 kb region between marker InDel11-5 and InDel11-9 on the long arm of chromosome 11, in which no gene leaded to leaf-color mutation had been mapped or cloned. Cloning and sequencing analysis revealed that, LOC_Os11g38040, which was predicted to encode an expressed protein, had a 9 bp segment deletion in the coding region of bgl11. Furthermore, the transgenic plants with wild-type gene LOC_Os11g38040were restored to normal phenotype. Accordingly, the gene (LOC_Os11g38040) was identified as the BGL11(t) gene. These results are very valuable for further study on BGL11(t)gene and illuminating the mechanism of chloroplast development in rice.

Electroless plating of Co―Zn―P thin film onto nanodiamond cores

Zhi Xiang Zheng,Rui Wang,Chun Ming Wang 한국물리학회 2011 Current Applied Physics Vol.11 No.2

The purpose of this article is to introduce the Co―Zn―P film coated on the nanodiamond cores by electroless deposition. The surface morphology, composition and magnetic performance of the coatings are studied with the help of transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), an energy dispersive X-ray detector (EDX) and the vibrating sample magnetometer (VSM). The results showed that the change of film composition and magnetic characteristics were dependant on the content of Cobalt, Zinc and Phosphorus. Evidently the coercive force was affected by the atomic composition of alloy film While the best proportion (the optimal composition mass ratio of Cobalt and Zinc was 1:4) in alloy film, the alloy film has good electromagnetic properties, even though the Phosphorus wt.%≥3%.

The Phytotoxic Effects of Selenium–Mercury Interactions on Root Growth in Brassica rapa (LvLing)

Zhi-Wei Bian,Jian Chen,Hui Li,Dan-Dan Liu,Li-Fei Yang,Yue-Lin Zhu,Wen-Li Zhu,Wei Liu,Zheng-Zheng Ying 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.3

Rapid industrial and agricultural development has dramatically increased the emission of selenium (Se) and mercury (Hg) into the environment. Combined soil pollution by Se and Hg poses a potential threat to crop production. However, no toxic effects of Hg–Se interactions on plants have been reported previously. In this study, we investigated the effects of Hg–Se interactions on biochemical and physiological indices in the roots of Brassica rapa (LvLing). Seedlings were treated hydroponically with solutions of mercury chloride (1 μM), sodium selenite (4 μM), or a combination of the two. Combined Hg+Se treatment significantly inhibited root growth, reduced root biomass, and enhanced reactive oxygen species (ROS) and malondialdehyde accumulation and led to a loss of plasma membrane integrity. The combined treatment increased glutathione peroxidase, glutathione S-transferase, and peroxidase activity, reduced superoxide dismutase activity, and had no effect on catalase activity. In addition, we detected increased glutathione concentrations in root tips and reduced ascorbic acid concentrations in the presence of Hg+Se relative to individual treatments with these elements. Thus, Hg–Se interactions enhanced oxidative injury, cell death, and phytotoxicity in B. rapa roots.