발기부전 검사에서 천연색 복합초음파촬영술과 야간음경발기검사의 일치도

송윤섭,구자현,김민의,이혜경,김두상,박영호,이남규 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

We intended to know that the result of color duplex ultrasonography corresponded to that nocturnal penile tumescence rigidity in the diagnosis of erctile dysfunction. We performed both color duplex ultrasonography and nocturnal penile tumescence rigidity in 15 erectile dysfunction men. Colr duplex ultrasonography was done with a 7 MHz. color Doppler unit after intracorporeal pharmacological injection and measured maximal arterail diameter, peak systolic velocity, end-diastolic velocity and the results were compared with rigidity and tumescence in nocturnal penile tumescence rigidity using RigiScan. The positive predictive value, negative predictive value, sensitivity, specificity, overall accuracy of color duplex ultrasonography was reasonable when the result of color duplex ultrasonography was compared with the that of nocturnal penile tumescence rigidity. According to this results, color duplex ultrasonography could not only predict but also correspond to nocturnal penile tumescence. Therefore, we suggest that color duplex ultrasonography can relpace nocturnal penile tumescence rigidity if psychogenic and neurogenic erectile dysfunction are distinguished by history and neurologic examination.

신고리 지역 기상관측을 통한 계절별 대기안정도 특성분석

송상근,김유근,오인보,정주희,조윤미,우경은,강태훈 부산대학교 환경문제연구소 2003 環境硏究報 Vol.21 No.-

In this study, we analyzed the characteristics of atmospheric stability to investigate local environment change at an atomic power plant in Gori region. The used data was obtained from field observation of major meteorological variables such as wind direction and speed, air temperature, relative humidity, and solar radiation using AWS (Automatic Weather System) in Gori region from February to September in 2003. As a result, when strong northerly winds (more than 4~6 m/s) and weak northwesterly winds (2~3m/s) blow, atmospheric stability was neutral and stable from winter to spring, respectively. Atmospheric stability (neutral) in summer was similar to that in two other seasons (winter and spring), but it was stable for weak southwesterly winds (2~3 m/s). Finally, atmospheric stability was neutral for strong winds (over 4~6 m/s) in fall without respect to wind direction, while that was stable for very weak winds (less than 2 m/s).

진단 기준에 따른 취학전 어린이의 중증 유아기 우식증 유병률 비교

임경욱,이광희,라지영,이동진,안소연,김지영,송지현,김윤희 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

연구목적은 중증 유아기 우식증에 대한 두 개의 정의를 진단 기준으로 하였을 때 각 기준에 따른 유병률이 일치하는 정도를알기 위함이었다. 연구대상은 익산시 어린이집 중에서 무작위로 추출한 7개 어린이집의 36개월 이상 71개월 이하 어린이401명 이었다. 치경과 탐침을 사용한 구강검사를 통하여 치아별 우식경험유치면 (dmfs)의 수를 조사하였으며 . 중증 유아기 우식증의 진단 기준은 (1) 상악 전치에 1개 이상의, 와동이 형성된 우식,상실(우식증에 의한), 충전 평활면이 있거나 (2) 우식,상실,충전 치면의 수가 3세는 4개 이상.4세는 5개 이상.5세는 6개 이상인 경우로 하였다. 상악 전치부 우식증에 의한 우식유병률과 우식경험유치면수(dmfs)에 의한 우식유병률은 3세에서 각각 26.7%. 28.9%, 4세에서 각각 32.4%, 30.4%, 5세에서 각각 39.4%, 41.3%, 전체에서 각각 35.4%, 35.9%으로서. 연령별로는 약간의 차이가 있었으나 전체에서는 차이가0.5%로서 거의 일치하였다. 따라서 중증 유아기 우식증의 진단은 두 가지 기준 중에서 어느 하나를 사용하더라도 비슷한 결과가 나을 것이라는 것을 확인할 수 있었다. The purpose of study was to compare the prevalences of severe early childhood cahes(S-ECC) using two different diagnostic criteria. The subjects of this study included 401 preschool children from 36 to 71 months of the randomly selected seven nurseries in Iksan city. Severe early childhood caries was defined as (1) 1 or more cavitated, missing (due to caries), or filled smooth surfaces in primary maxillary anterior teeth or (2) a decayed, missing, or filled score of ≥4 (age 3), ≥5 (age 4), or ≥6 (age 5) surfaces. The prevalence determined by maxillary anterior caries and the prevalence determined by dmfs were 26.7%, 28.9% in 3 years, 32.4%, 30.4% in 4years. 39.4%, 41.3% in 5 years, and 35.4%, 35.9% in all, respectively. The results suggest that the two diag-nostic criteria of the severe early childhood caries yield almost the same results.

절수에 의한 mongolian gerbil의 체중 변화에 관한 연구

이경열,송치원,김무강,조성환,류시윤,김상근,박배근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Recently, the mongolian gerbils have been used in neurology and the study of water metabolism because of their easy onset of epileptic seizure and typical resistance to water deprivation. The sebaceous glands which are under the control of the gonadal hrmones are differently developed from the other laboratory animals. However the physiological data on the changes of body weight following water deprivation in mongolian gerbils is not available until now. In order to investigate the effect of the water deprivation on the body weight, the animals were deprived of water for 15 days. The body weights were measured daily by the torsion balance. The mean, the standard deviation, the standard error and the coefficient of variance of the body weights were calculated. The correlation was examined. The potential regression equation was y = 61.5458 - 2.0533 * x + 0.0487 * x^2. The regression coefficient was 0.993. The results obtained were as follows : 1. The body weights of the mongolian gerbils were continuously decrased from the 1st day to the 15th day after the water deprivation. 2. The mean body weight on the water deprivation was 63.0±7.00g and that at the 15th day was 41.4 ± 5.42g. 3. The coefficients of the variance showed the highest (13.09) at the 15th day and the lowest (10.10) at the 10th day. the coefficients of the variance kept decreasing until the 10th day and thereafter gradually increased until the 15th day. 4. The changing rate of the body weight kept continuously decreasing from the 1st day to the 15th day showing the daily loss of 1.0 - 3.3%. 5.The deviations (M - QBW) between the measured values (m) and the theorical values (QBW) using the regression equation showed the highest (1.494) at the 1st day and the lowest (0.053) at the 13th day. 6. The sum of the devitions was 0.179 and that of the deviation square was 1.15. The sum of the deviation rates between the mesaured body weights and the theorical ones was 0.484. 7. The daily body weight loss showed the highest at the 1st day by 2.053g and the lowest at the 15th day by 0.591g. 8. The specific body weight changing rates which were the rate of the body weight changing velocity to the changed body weight were decreased from 0.033% at the 1st day to 0.014% at the 15th day.

Carrageenan 유도 관절염에 대한 灸律 藥鍼의 진통작용

구성태,신종근,최윤영,송정방,김재효,김경식,손인철 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

Objectives: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased nitric oxide(NO) and inducible NO synthase (iNOS) generation in cellular experiments. Methods: Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. Diluted moxi-tar was single injected in several acupoints or combined with electroacupuncture (1 ms, 2 Hz, and 2 mA) into contralateral ST36 acupoint for 30 min to assess the synergic effects. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord were examined on a rat model of carrageenan-induced arthritis. Results : After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Moxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Moxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by moxi-tar. Moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that moxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

Transdermal delivery of low molecular weight heparin loaded in flexible liposomes with bioavailability enhancement: comparison with ethosomes

Song, Yun-Kyoung,Hyun, Seo Yeon,Kim, Hyung-Tae,Kim, Chong-Kook,Oh, Jung-Mi Informa UK, Ltd. 2011 Journal of microencapsulation Vol.28 No.3

<P>Low molecular weight heparin (LMWH)-loaded flexible liposomes (flexosomes) were formulated for transdermal delivery, and their physicochemical and pharmacokinetic parameters were compared with LMWH-loaded ethosomes. Flexosomes had similar particle size compared with ethosomes, but their deformability was higher than that of ethosomes (76.7%% <I>vs</I>. 46.8%%). <I>In ?vitro</I>, flexosomes demonstrated 2.6-fold higher permeability coefficient than ethosomes. In comparison to LMWH aqueous solution, skin deposition of flexosome increased 3.2-fold, while that of ethosome increased only 2.0-fold. <I>In ?vivo</I>, after the topical application of flexosome to hairless mouse, [anti-Xa]<SUB>max</SUB> was 1.11 ??IU/mL, while ethosomes showed only 0.32 ??IU/mL. Moreover, AUC<SUB>0-??24 ??h</SUB> of flexosomes was 2.5-fold higher than ethosomes. In conclusion, the enhanced skin permeation and bioavailability of LMWH can be achieved with flexosomes in comparison with ethosomes. The LMWH transdermal delivery <I>via</I> flexosomes has the potential to replace the parenteral dosage forms for the treatment of venous thromboembolism, pulmonary embolism and cardiovascular events.</P>

Role of CaBud6p in the Polarized Growth of Candida albicans

Song Yun-Kyoung,Kim Jeong-Yoon The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.3

Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candide albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-Hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.

Determination of dexamethasone in human plasma using high-performance liquid chromatography

Song Yun-Kyoung,Park Jeong-Sook,Kim Jin-Ki,Kim Chong-Kook 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.1

Determination of glimepiride in human plasma using semi-microbore high performance liquid chromatography with column-switching

Kim Chong-Kook,Kim Jin-Ki,Kim Bae-Chan,Park Jeong-Sook,Hwang Hye-Ryung,Maeng Jeong-Eun,Song Yun-Kyoung 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.2