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Yun-Fang Qian,Qing Xiong,Sheng-Ping Yang,Jing Xie 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.6
Melanosis is major problem of crustaceans duringtheir rigor mortis storage. This study for the firsttime was designed to optimize the formula of preservativesto maintain the color feature of Pacific white shrimp usingresponse surface methodology. A three-factors-three-levelsBox-Behnken design was applied to evaluate the effect ofchitosan, citric acid and L-cysteine on color features (L*,a*, b* and DE) of Pacific white shrimp. It was found thatthe increasing rate of DE was retarded by the higher concentrationsof chitosan, citric acid and L-cysteine in acertain range. The optimal formula for inhibiting theincrease of DE was 1.36% chitosan, 0.47% citric acid and0.31% L-cysteine. Under the optimal pretreated conditions,the predicted DE of shrimp after 8 days of storage was14.59, close to the measured values (14.49). These resultsindicated that the optimal combined preservatives couldretard the decrease of lightness and the aggregation of DEand melanosis effectively, and might be a potentialapplication for retarding melanosis and extending shelf lifeof Pacific white shrimp.
Determining Inspection Intervals for One-shot Systems with Support Equipments
Qian Qian Zhao,Won Young Yun 대한산업공학회 2015 대한산업공학회 추계학술대회논문집 Vol.2015 No.11
In this paper, we consider one-shot systems comprise one-shot devices and support equipments. The one-shot devices are stored for long periods of time, and its failure is detected only upon inspection. Support equipments are necessary for the operation of one-shot devices, and are maintained immediately upon failure. This paper deals with an inspection schedule problem for one-shot systems with support equipment and limited maintenance resources. Interval availability and life cycle costs are used as optimization criteria, and are estimated by simulation. Therefore, we aim to determine near-optimal inspection intervals of one-shot systems that minimize the expected life cycle cost and satisfy the target interval availability during inspection periods. An estimation of distribution algorithm (EDA) is proposed to find near-optimal solutions, and numerical examples are studied to investigate the effects of various model parameters.
Kai Qian,XiaoHai Gong,Bo Guan,SuPing Wu,JingJing Zhang,Jing Qian,YanFei Cai,Yun Chen,ZuoYing Duan,Xin Ma,HuaZhong Li,Jian Jin 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Glucagon-like peptide-1 (GLP-1) was a potential therapeutic drug for type II diabetes, mainly because of the stimulatory effect on insulin secretion under condition of high blood glucose. We used PCR to obtain a recombination gene, GGH, in which two GLP-1 (GLP-1A2G) mutants were connected in series and then fused to the N terminal of human serum albumin. The fusion gene was inserted into pGAPZaA plasmid with Saccharomyces cerevisiae α- factor secretion signal sequence, and was expressed by the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The engineered strain was constructed by integrating the recombinant plasmid pGAPZαA/GGH into the genome of Pichia pastoris GS115. Genome PCR and western blot showed that the recombinant P. pastoris successfully expressed the fusion protein GGH. The yield of GGH reached 78 mg/L after 72 h fermentation in a flask, using glucose as the optimal carbon source. Fed-batch fermentation was investigated in a 5 L bioreactor, and the expression level of GGH reached 246 mg/L in 52 h. The fusion protein GGH was purified in four steps, and the final purity was 96.1%. The in vitro bioactivity of GGH was the same as that expressed in P. pastoris by the AOX1 promoter. This study described an efficient way to express GGH fusion protein in P. pastoris using GAP promoter, fermentation was easier to control without carbon source change and fermentation time was 20 h less than AOX1 promotercontrolled GGH fermentation.
Spare Parts Provisioning under Multi-Echelon and Multi-Level Systems
Won Young Yun,Wonseok Jeon,Qian Qian Zhao 한국신뢰성학회 2018 신뢰성응용연구 Vol.18 No.4
Purpose: In this paper, we consider a spare parts allocation problem for multi-level systems with two-echelon repair systems. The system consists of multi-units (components and modules) and has a multi-level structure. The (S-1, S) inventory policy is considered to order spare parts. Methods: We determine the line replaceable units (LRUs) and these maximum spare parts storage levels S in echelon sites that minimize the life cycle cost and satisfy the target system availability. The near-optimal solutions are obtained by a genetic algorithm and simulation. The genetic algorithm is used to generate alternative solutions and simulation is used to evaluate the alternative solutions. Results: We investigate the effect of parameters on the near-optimal solutions in numerical examples. The results show that as the target system availability increases, the level of LRU is increased because shorter replacement time leads to increase the system availability. Conclusion: This study shows that the variation of target system availability affects the results of the near optimal solutions. For further studies, we will consider inventory policy of depot (s, S) and the repair of failed LRU.
Huan-Huan Gao,Nan Xu,Hao Chen,Qian Liu,Qian-Yun Pu,Dong-Yun Qin,Yi-Fan Zhai,Yi Yu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
Drosophila suzukii (Diptera: Drosophilidae) is one of the very few Drosophila species which are able to lay eggs and feed on healthy ripening fruit. Adults and larvae are all able to obtain the nutrition in the decaying food, which will generate many microorganisms. However, the relationship between D. suzukii and microorganism is poorly understood. In this study, 13 species of fungi were identified in an artificial diet fed by D. suzukii. Short-term and continuously life table experiments were conducted to determine the impact of three of these fungus species including Geotrichum candidum, Talaromyces minioluteus and Actinomucor elegans on the growth and development of Drosophila suzukii. Results revealed that, compared to the control, G. candidum, T.minioluteus and A. elegans increased the mortality of D. suzukii adults in the short time, while extended the developmental time of pupal D. suzukii by 18.00%, 16.22% and 26.44%, respectively in the life table experiment. T. minioluteus reduced the total longevity of D. suzukii by 15.52%, while A. elegans enhanced the total longevity by 25.96%. G. candidum and A. elegans increased the fecundity bymore than two folds. T. minioluteus elongated the mean generation time (T) by 31.34%, whereas G. candidum shortened it by 15.26% but increased the net reproductive rate (R0) by 217.76%, intrinsic rate of increase (r) by 88.89% and finite rate of increase (λ) by 9.17%. It was concluded that G. candidumand A. elegans significantly had the beneficial effect on the growth and reproduction of D. suzukii, however, T. minioluteus had the negative effect on D. suzukii. Our results could provide a new integrated pest management strategy for D. suzukii which would be discussed in this study.
Qian Ding,Xiao-Li Xie,Miao-Miao Wang,Jie Yin,Jin-Mei Tian,Xiao-Yu Jiang,Di Zhang,Jing Han,Yun Bai,Zi-Jin Cui,Hui-Qing Jiang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
The clearance of activated hepatic stellate cells (HSCs) by apoptosis is critical for the reversibility of hepatic fibrosis. Mitochondrial homeostasis is regulated by mitophagy, which is an efficient way of clearing injured mitochondria that plays an important role in apoptosis. However, the role of mitophagy in apoptosis in HSCs and hepatic fibrosis is still unclear. Here, we show that mitophagy is enhanced in parallel with increased apoptosis in hepatic stellate cells during the reversal of hepatic fibrosis. The inhibition of mitophagy suppressed apoptosis in HSCs and aggravated hepatic fibrosis in mice. In contrast, the activation of mitophagy induced apoptosis in HSCs. Furthermore, we confirmed that BCL-B, which is a member of the BCL-2 family, is a regulator mediating mitophagy-related apoptosis. The knockdown of BCL-B resulted in increased apoptosis and mitophagy in HSCs, while the overexpression of BCL-B caused the opposite effects. BCL-B inhibited the phosphorylation of Parkin (a key regulator of mitophagy) and directly bound phospho-Parkin. Altogether, enhanced mitophagy promotes apoptosis in HSCs during the reversal of hepatic fibrosis. BCL-B suppresses mitophagy in HSCs by binding and suppressing phospho-Parkin, thereby inhibiting apoptosis. BCLB- dependent mitophagy is a new pathway for the regulation of apoptosis in HSCs during the regression of hepatic fibrosis
Correction to: Amelioration of radiation‑induced liver damage by p-coumaric acid in mice
Yun-Hong Li,Jiang-Xue Wu,Qian He,Jia Gu,Lin Zhang,Hao-Zhi Niu,Xin-Wen Zhang,Han-Ting Zhao,Jia-Ying Xu,Li-qiang Qin 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.5
In the original publication, incorrect versions of Figs. 2 , 3 ,4 and 5 were published. Specifi cally, the arrows in Figs. 2 , 3and 4 were moved outside the representative images, and theFig. 5 was wrongly replaced by another fi gure. The correctversion of Figs. 2 , 3 , 4 , and 5 , were shown below.