Basic, HCCbasic : PO-19 ; CTHRC1 is a potential biomolecular marker in human hepatocellular carcinoma

( Yun Peng Wang ),( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( Hua Lee ),( Seong Hun Kim ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: CTHRC1 is a 30 kDa secreted protein that has the ability to inhibit collagen matrix synthesis. CTHRC1 expression in breast cancer and colorectal cancer is associated with cancer tissue invasion and metastasis. But, the functional significance of CTHRC1 expression in hepatocellular carcinoma (HCC) is unknown. So the purpose of this study is to investigate whether CTHRC1 is a biomolecular marker in human hepatocellular carcinoma. Methods: CTHRC1 protein expression was investigated by Immunoblotting analysis in HCC cell lines and HCC tumor tissues, respectively. The expressions of CTHRC1 in HCC tissues were also evaluated by immunohistochemical staining according to tumor differentiaton and stage. CTHRC1 levels were assessed in serum of HCC patients by Western blot analysis. Colony formation was assessed in SH-J1 and HLK3 cells. Results: Endogenous and ectopic expression of CTHRC1 was mainly localized in the cytoplasm of the tumor cells. The immunohistochemical staining showed that the expression of CTHRC1 was negligible in normal liver tissue, but most tumors were immunoreactive. CTHRC1 was highly expressed in tumor tissues compares with non - tumor tissues, but barely expressed in HCC cells. We also found that secreted CTHRC1 was abundantly present in the serum of the patients with early and advanced HCCs, and absent with normal liver. Colony generation assay revealed that CTHRC1 overexpression increased colony number by two-folds. Conclusions: These results suggest that CTHRC1 protein maybe useful as a potential biomolecular maker for HCC.

Basic, Research : Lipocalin-2 Negatively Modulates the EMT in Hepatocellular Carcinoma through the EGF (TGFbeta1)/Lcn2/Twist1 Pathway

( Yun Peng Wang ),( Goung Ran Yu ),( Mi Jin Lee ),( Sang Yeop Lee ),( In Sun Chu ),( Sun Hee Leem ),( Dae Ghon Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Backgrounds/Aims: Lipocalin-2 is preferentially expressed in hepatocellular carcinoma. However, the functional role of Lcn2 in HCC progression is still poorly understood, particularly with respect to its involvement in invasion and metastasis. The purpose of this study was to investigate whether Lcn2 is associated with the EMT change of HCC and to elucidate its signaling pathway. Methods: Lcn2 mRNA expression was confirmed by RT-PCR analysis. Lcn2 protein expression was investigated by immunohistochemistry or Western blot analyses. The proliferation, migration, and invasion ability were measured by MTT, wound healing and invasion assays in HCC cells (SH-J1) stably expressing Lcn2. Tumor growing and metastasis by Lcn2 was tested in vivo animal model. Results: Lcn2 is preferentially expressed in well differentiated HCC versus liver cirrhosis tissues, and its expression is positively correlated with the Edmondson differentiation grade of HCC. The characteristics of EMT are reversed by adenoviral transduction of Lcn2 into SH-J1 cells, including the down-regulation of N-cadherin, vimentin, a-SMA, and fibronectin, and the concomitant up-regulation of CK8, CK18, and desmoplakin I/II. Knock-down of Lcn2 by shRNA in HKK-2 cells highly expressing Lcn2 was associated with EMT change. EGF or TGF-β1 treatment resulted in down-regulation of Lcn2, subsequently accompanied by Twist1 expression and EMT change in HCC cells. Stable Lcn2 expression in HCC cells reduced Twist1 expression, led to inhibition of cell proliferation and invasion in vitro, and suppressed tumor growth and metastatic ability in a mouse model. Furthermore, EGF or TGF-β1 treatment barely changed EMT marker expression in the SH-J1 cells ectopically expressing Lcn2. Ectopic expression of Twist1 induced EMT marker expression even in cells expressing Lcn2, indicating that Lcn2 functions downstream of growth factors and upstream of Twist1. Conclusions: These findings suggest that Lcn2 can negatively modulate the EMT change in HCC cells through the EGF (or TGF-β1)/Lcn2/Twist1 pathway. Thus, Lcn2 may be a candidate metastasis suppressor and a potential therapeutic target in HCC.

Basic, HCC basic : PO-19 ; CTHRC1 is a potential biomolecular marker in human hepatocellular carcinoma

( Yun Peng Wang ),( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( Hua Lee ),( Seong Hun Kim ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: CTHRC1 is a 30 kDa secreted protein that has the ability to inhibit collagen matrix synthesis. CTHRC1 expression in breast cancer and colorectal cancer is associated with cancer tissue invasion and metastasis. But, the functional significance of CTHRC1 expression in hepatocellular carcinoma (HCC) is unknown. So the purpose of this study is to investigate whether CTHRC1 is a biomolecular marker in human hepatocellular carcinoma. Methods: CTHRC1 protein expression was investigated by Immunoblotting analysis in HCC cell lines and HCC tumor tissues, respectively. The expressions of CTHRC1 in HCC tissues were also evaluated by immunohistochemical staining according to tumor differentiaton and stage. CTHRC1 levels were assessed in serum of HCC patients by Western blot analysis. Colony formation was assessed in SH-J1 and HLK3 cells. Results: Endogenous and ectopic expression of CTHRC1 was mainly localized in the cytoplasm of the tumor cells. The immunohistochemical staining showed that the expression of CTHRC1 was negligible in normal liver tissue, but most tumors were immunoreactive. CTHRC1 was highly expressed in tumor tissues compares with non - tumor tissues, but barely expressed in HCC cells. We also found that secreted CTHRC1 was abundantly present in the serum of the patients with early and advanced HCCs, and absent with normal liver. Colony generation assay revealed that CTHRC1 overexpression increased colony number by two-folds. Conclusions: These results suggest that CTHRC1 protein maybe useful as a potential biomolecular maker for HCC.

CYP1A1 Genetic Polymorphisms and Risk for Esophageal Cancer: a Case-control Study in Central China

Yun, Yu-Xia,Wang, Yan-Ping,Wang, Peng,Cui, Li-Hong,Wang, Kai-Juan,Zhang, Jian-Ying,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

The purpose of this study was to evaluate the associations of CYP1A1 genetic polymorphisms with the risk of developing esophageal cancer (EC). A case-control study was carried out in a Chinese population in which 157 hospital based EC cases and 157 population based healthy controls with 1:1 match by age and sex were included. PCR based restriction fragment length polymorphisms (PCR-RFLP) were used to detect genotypes in case and control groups. For the CYP1A1 Ile/Val polymorphism, comparing with wild genotype Ile/Ile, both the heterozygote genotype Ile/Val and the combined variant genotype Ile/Val+Val/Val increased the risk of esophageal cancer (OR: 2.05, 95%CI: 1.19-3.54, OR: 1.86, 95%CI: 1.11-3.12). No significant association was found between the CYP1A1 MspI polymorphism and EC. According to analysis of combined genotypes, the TC/AG combined genotype which contained both variant alleles of these two polymorphisms increased the risk of developing EC (OR: 2.12, 95%CI: 1.16-3.85). Our results suggested that genetic polymorphisms of CYP1A1 may increase the susceptibility to EC.

Expression of Twist2 in human hepatocellular carcinoma and its functional significance (초)

( Yun Peng Wang ),( Mi Jin Lee ),( Xiang Dan Cui ),( Gyung Ran Yu ),( Dae Ghon Kim ) 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.3(S)

Evolution of ALPPS: The Simpler, Safer and Effective One---TELPP

( Shu You Peng ),( Xu An Wang ),( Cong Yun Huang ),( You Yong Zhang ),( Jiang Tao Li ),( De Fei Hong ),( Xiu Jun Cai ),( Yi Fang Wang ),( Xiao Liang ),( Jian Wei Wang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The characteristic of associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) carries high mortality and morbidity. There is room for improvement. We suggest Terminal Branches Portal Vein Embolization (TBPVE) as a way to compart the liver. As a result, only a single surgical operation is required.This method is termed Terminal branches portal vein Embolization Liver Partition Planned hepatectomy (TELPP). Methods: Patients with unresectable primary or metastatic liver tumor were performed with TELPP. The procedure of TELPP was that in addition to PVE, embolization agent was infused to the terminal branches of portal vein of S5,S8 or S4. CT scan was taken one or two weeks later, and standard liver volume(SLV), FLR and FLR/SLV are calculated. Two weeks later when the FLR and liver function is appropriate, open or laparoscopic hepatectomy is performed. Results: The study included 11patients including hepatocellular carcinoma: n =8, intrahepatic cholangiocarcinoma: n = 1, hilarcholangiocarcinoma: n =1, colorectal liver metastasis: n =1. After a waiting period of 14 days, the volume of theFLR had increased from 382mlto 578ml, representing a median volume increase of 51% (range =32.5%-86.7%). Of the 11patients with hepatectomy, right hemihepatectomy (n=2), extended right hemihepatectomy (n=5), right trisecmentectomy(2), extended left hemihepatectomy (n=1) and left trisecmentectomy(1). No patient died, and no serve perioperative morbidity occurred. Conclusions: ALPPS and all modifications need two-stage operations with a high morbidity and mortality rate. It seems that TELPP is very promising. It has the merit of ALPPS as extraordinarily rapid increasement of FLRvolume, yet the morbidity and mortality is much lower, owing to the fact that unlike ALPPS, there is no two liver raw surfaces left behind in the abdominal cavity to produce bile leak, as only single surgical operation is required

Covered Stenting Is an Effective Option for Traumatic Carotid Pseudoaneurysm with Promising Long-Term Outcome

Wang, Kai,Peng, Xiao-xin,Liu, Ao-fei,Zhang, Ying-ying,Lv, Jin,Xiang, Li,Liu, Yun-e,Jiang, Wei-jian The Korean Neurosurgical Society 2020 Journal of Korean neurosurgical society Vol.63 No.5

Objective : Covered stenting is an optional strategy for traumatic carotid pseudoaneurysm, especially in malignant conditions of potential rupture, but the long-term outcomes are not clear. Our aim was to determine if covered stenting is an effective option for traumatic carotid pseudoaneurysm with promising long-term outcomes. Methods : Self-expanding Viabahn and balloon-expandable Willis covered stents were separately implanted for extra- and intracranial traumatic carotid pseudoaneurysm. The covered stent was placed across the distal and proximal pseudoaneurysm leakage under roadmap guidance. Procedural success was defined as technical success (complete exclusion of the pseudoaneurysm and patency of the parent artery) without a primary end point (any stroke or death within 30 days after the procedure). Long-term outcomes were evaluated as ischemic stroke in the territory of the qualifying artery by clinical follow-up through outpatient or telephone consultation and as the exclusion of the pseudoaneurysm and patency of the parent artery by imaging follow-up through angiography. Results : Five patients with traumatic carotid pseudoaneurysm who underwent covered stenting were enrolled. The procedural success rate was 100%. No ischemic stroke in the territory of the qualifying artery was recorded in any of the five patients during a mean clinical follow-up of 44±16 months. Complete exclusion of the pseudoaneurysm and patency of the parent artery were maintained in all five patients during a mean imaging follow-up of 39±16 months. Conclusion : Satisfactory procedural and long-term outcomes were obtained, suggesting that covered stenting is an effective option for traumatic carotid pseudoaneurysm.

Human Papillomavirus (HPV) Infection in Women Participating in Cervical Cancer Screening from 2006 to 2010 in Shenzhen City, South China

Wang, Yue-Yun,Li, Li,Wei, Sheng,Peng, Ji,Yuan, Shi-Xin,Xie, Jian-Sheng,Liu, Zhi-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Purpose: Human papillomavirus (HPV) infection plays an important role in the development of cervical cancer, but the prevalence of HPV infection in women of Shenzhen city remains unclear. The present study was performed to describe the change of cervical HPV infection in females who participated in voluntary cervical cancer screening from 2006 to 2010 in Shenzhen city, China. Methods: A total of 4, 413 women were recruited. HPV infections were genotyped by polymerase chain reaction (PCR) and reversed dot blot hybridization in Shenzhen Maternity and Child Health Hospital. Results: The prevalence of HPV infection was 13.8%. The five most commonly found HPV types were HPV16 (3.47%), HPV58 (1.68%), HPV33 (1.38%), HPV43 (1.36%) and HPV18 (1.27%). The secular trends of major HPV type-specific were diverse. Among of them, the prevalence of HPV18 increased sharply while others increased slowly or even decreased in the period. The change of total HPV, single HPV and multiple HPV infection were similar during the five years. Conclusions: Our findings suggested that HPV infection is common with HPV16 and HPV 58 as the primary subtypes in women in Shenzhen city.The prevalence of HPV 18 infection is increasing faster than any others, which will lead it to be one of the main subtypes in this city in the future.

The MDM2 SNP309T>G Polymorphism Increases Bladder Cancer Risk among Caucasians: a Meta-analysis

Wang, Huai-Gao,Wu, Qing-Yun,Zhou, Hui,Peng, Xin-Sheng,Shi, Meng-Jie,Li, Jie-Mei,Zhou, Yan-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Published studies have evaluated associations between the MDM2 SNP309T>G polymorphism and bladder cancer susceptibility. However, these generated inconsistent results. The aim of the present investigation was to quantify the strength of association between MDM2 SNP309T>G polymorphism and bladder cancer risk by conducting a meta-analysis. We searched PubMed and Embase for related studies that had been published in English before April 1, 2014 and associations were assessed by summarizing the odds ratios (ORs) with the corresponding 95% confidence intervals (CIs). Five case-control studies with a total of 972 cases and 1,012 controls were finally identified to be eligible for the meta-analysis. Overall, the results indicated that there was no significant association between the MDM2 SNP309T>G polymorphism and bladder cancer risk (for the allele model G vs. T: OR=1.08, 95% CI 0.85-1.36, p=0.54; for the co-dominant model GG vs. TT: OR=1.20, 95% CI 0.74-1.93, p=0.46; for the dominant model GG+GT vs. TT: OR=0.98, 95% CI 0.80-1.20, p=0.83; for the recessive model GG vs. GT+TT: OR=1.20, 95% CI 0.83-1.74, p=0.33). However, on subgroup analysis by ethnicity, significant associations were found in Caucasians in three models (for the allele model G vs. T: OR=1.41, 95% CI 1.10-1.81, p=0.006; for the co-dominant model GG vs. TT: OR=2.16, 95% CI 1.28-3.63, p=0.004; for the recessive model GG vs. GT+TT: OR=2.06, 95% CI 1.31-3.22, p=0.002). In summary, the present meta-analysis provides evidence that the genotype for the MDM2 SNP309T>G polymorphism may be associated with genetic susceptibility to bladder cancer among Caucasians.

How Does Leadership Influence Follower Creativity? - The Role of Follower Identifications and Perceived Organizational Support -

Wang Huan Huan,Zhang Hong Peng,Kim Jong Kwan,Lee Yun Kyeong 한국인적자원관리학회 2018 인적자원관리연구 Vol.25 No.5

오늘 날 4 차 산업혁명으로 명명되는 기술적 융합과 디지털 신기술의 태동으로 인간의 노동을 대체할 수 있는 인공지능의 등장은 인간의 가치가 창의적인 사고에 있음을 다시금 깨닫게 하고 있다. 이와 같은 환경의 병화에서 많은 조직과 기업들은 창의적인 사고, 지식 공유 등을 통하여 고유한 가치를 개발하고 발전시키기 위해 노력하고 있다. 조직 내 창의성은 단순히 창의적인 개인으로 인하여 만들어 질 수 있는 것이 아니라, 구성원이 창의적일 수 있는 조직문화가 중요하다. 많은 연구에서는 리더의 역할이 조직문화 조성에 중요한 기반으로 보고 있다. 본 연구에서는 변혁적 리더십과 거래적 리더십의 개념 및 구성요소에 대한 명확한 설명을 토대로 팔로어의 창의성과 리더십의 관계를 검증하고자 한다. 기존에 연구된 리더십과 창의성의 관계에 팔로어의 정체성을 매개변수로, 지각된 조직지원인식을 조절변수로 하여 보다 포괄적인 관계를 알 수 있을 것으로 생각한다. 연구를 위해 중국 북경과 상하이의 기업을 대상으로 설문을 실시하여 242 부의 설문지를 통계에 사용하였다. 가설검증 결과 변혁적 리더십과 거래적 리더십은 오두 팔로어의 창의성에 긍정적인 영향이 있는 것으로 나타났다. 또한 팔로어의 정체성이 리더십과 팔로어의 창의성의 관계를 매개하고 있으며, 리더십과 팔로어의 관계적 정체성은 지각된 조직지원인식에 의해 조절되고 있었다. 이와 같은 연구결과를 바탕으로 연구의 시사점, 한계점 및 향후의 연구 방향이 제시되었다. The importance of creativity to an organization and society is undeniable, simultaneously, leadership is a key factor affecting the creativity of followers within an organization. Based on the clarification of the concepts and dimensions of transformational leadership and transactional leadership, this paper examined follower identifications as a mediator and employees’ perceptions of organizational support as a moderator in the relationship between leadership and follower creativity. Using a sample of 242 employees who engaged in manufacturing working in Beijing and Shanghai, China, we found that most of our hypotheses were supported. Specifically, results showed that follower identifications mediate the positive influence relationship between leadership on follower creativity, and follower’s perceived organizational support moderate both transformational leadership and transactional leadership in the relationship with follower relational identification. Implications of the findings, Limitations of the study and directions for future research are discussed.