Potassium alum과 비우식성 감미료의 병용이 Streptococcus mutans의 성장에 미치는 영향

김윤정,차정단,김지영,김강주,이흥수 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.3

Traditionally, Koreans have used potassium alum by mouth rinse when they suffered from stomatitis. This study was carried out to investigate the combined effect of potassium alum with aspartame, sorbitol and xylitol on the growth of Streptococcus mutans (S. mutans). The relative growth ratio of S. mutans JC-2 cultured at different concentrations of potassium alum and different concentrations of aspartame, sorbitol and xylitol was examined. The combined effect of different concentrations of potassium alum with 0.5% and 10.0% of aspartame, sorbitol and xylitol was examined. The lowest minimum inhibitory concentration of potassium alum was 5.0mg/ml against S. mutans JC-2. The relative growth ratio of potassium alum on S. mum JC-2 was affected at the concentration of 2.0mg/ml. The growth of S. mutans JC-2 decreased at the concentration of 10.0% of aspartame and sorbitol. Also, a combination of potassium alum and sugar substitutes was more effective against S. mum than the use of one sugar substitute alone. The inhibitory effect of potassium alum and sugar substitutes on S. mutans may be an important mechanism of caries prevention by potassium alum.

OB-06 : Enhancing neuronal differentiation of human umbilical cord blood-mesenchymal stem cell by three-dimensional collagen hydrogel and induced neurotrophic factors

( Yun Dan Kang ),( Soo Mi Kim ),( Ye Kyu Park ),( Jong Soo Kim ),( Jin Wan Park ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

In this study, collagen hydrogel has been evaluated as a stem cell carrier by a number of different criteria. First, we tested whether collagen hydrogel induced the secretion of neurotropic factors from UBC-MSCs. Second, by utilizing a 3-D co-culture with neuronal precursors, we tested the efficiency with which the neurotropic factors from UBC-MSCs were delivered to nearby neural precursors. We first tested whether the cell population that proliferated attached to the bottom of the plastic culture dish out of a mononuclear fraction obtained from the umbilical cord blood was enriched with mesenchymal stem cells by flow cytometric analysis. Next, we performed a RT-PCR analysis to examine neurotrophic factor and growth factor expression in the mesenchymal cell-enriched cell population of the umbilical cord blood. And we compared the 2-D culture of UBC-MSCs with the 3-D culture of UBC-MSCs in terms of neurotropic factor expression. We conducted co-culture experiments to examine whether UBS-MSCs in a collagen gel were capable of stimulating outgrowth and neuronal differentiation of human neuronal precursor cells (hNPCs). In comparison with 2-dimensional (2-D) liquid culture, 3-D culture of UCB-MSCs in a collagen-based hydrogel significantly enhanced the expression of various neutropic factors including neurotrophin-4, nerve growth factor, brain-derived neurotropic factor, and ciliary neurotropic factor as verified by the gene and protein analysis. In addition, a co-culture with human neuronal precursor cells significantly enhanced neurite outgrowth and neural differentiation of hNPCs as evidenced by enhanced expression of β-III tubulin and NeuN. Our results demonstrate that the collagen-based hydrogel is excellent at inducing the neurotrophic factor release from UBC-MSCs and providing a tropic support to hNPCs, suggesting the possibility that 3-D UBC-MSC culture in the collagen hydrogel could be a promising strategy for nerve regenerative medicines.

자궁 내 태아발육부전에 의한 성인 시 후유증

강윤단(Yun Dan Kang) 대한여성건강학회 2007 여성건강 Vol.8 No.1

Low birthweight in relation to the length of gestation, is now known to be associated with increased rates of coronary heart disease and the related disorders stroke, hypertension and type 2 diabetes. These associations extend across the whole range of birthweight, which implies that normal variations in nutrient delivery to the fetus have profound long-term effects. The associations are thought to reflect the body's plasticity during development, by which its structure and function can be permanently changed by the intrauterine and early postnatal environment. Slow growth during infancy and rapid weight gain after the age of two years exacerbate the effect of slow fetal growth. Cardiovascular disease and type 2 diabetes arise through a series of interactions between environmental influences and the pathways of development that precede them.

산전 초음파로 진단된 태아 두개강 내 출혈

강윤단 ( Yun Dan Kang ),정윤희 ( Yun Hee Jeong ),진건 ( Keon Jin ),박만철 ( Man Chul Park ) 대한주산의학회 2008 Perinatology Vol.19 No.2

Fetal intracranial hemorrhage is quite rare. Antenatal fetal intracranial hemorrhage may occur spontaneously, or in association with various maternal or fetal conditions. Currently, antenatal fetal intracranial hemorrhage may be diagnosed by imaging techniques including ultrasonography and less frequently, magnetic resonance imaging (MRI). We report a case of spontaneous fetal intracranial hemorrhage that was diagnosed antenatally in the third trimester with a brief review of literatures.

임신 중 발병한 문맥혈전증

강윤단 ( Yun Dan Kang ) 대한주산의학회 2015 大韓周産醫學會雜誌 Vol.26 No.3

Portal vein thrombosis complicating pregnancy is a rare occurrence. The etiology of portal vein thrombosis is highly diverse. A 31-year-old multigravida was diagnosed with acute portal vein thrombosis at 12 weeks of gestation by ultrasound examination. She had epigastric and left upper quadrant pain, but there was no significant medical or surgical illness in the past. Laboratory studies showed no evidence of a thrombophilia. She was managed with anticoagulants and labor was induced at 38 weeks because of premature rupture of membranes. She delivered a healthy neonate without any complications. It seems that the cause of this thrombotic event was the hypercoagulable state of pregnancy.

임상 ; 태반 기능장애가 악화된 경증 전자간증 산모에서 입원 시 실시한 체계적 평가

강윤단 ( Yun Dan Kang ),유우정 ( Woo Jeong Yu ),박만철 ( Man Chul Park ) 대한주산의학회 2007 Perinatology Vol.18 No.3

목적: 체계적 평가에서 경증 전자간증으로 판명되었던 산모를 대상으로, 태반 기능장애가 악화되지 않았던 군과 분만 전에 태반 기능장애가 악화된 군으로 나누어 이들 사이에 체계적 평가 검사 결과가 어떤 차이를 보이나 알아보기 위함이다. 방법: 입원하여 체계적 평가 실시 후 경증 전자간증으로 판명되었던 산모 76명 중, 분만까지 태반 기능장애가 악화되었던 산모는 28예이었고, 악화되지 않았던 산모는 48예이었다. 악화군의 판단 기준은 초음파 검사에서 양수지수가 5 cm 이하로 양수 양이 감소하였거나 진통 중 전자 태아심박 감시 장치에서 후반 심박동 감소 또는 다양성 심박동 감소의 반복, 지속적인 빈맥이 변동성 소실과 함께 나타나거나, 장기적인 심박동 감소가 나타났던 경우로 하였다. 중증 전자간증으로 발전된 경우도 악화군에 포함시켰다. 결과: 혈중 총 단백질의 농도 및 24시간 단백뇨의 양은 차이가 없었으나, 알부민 농도는 악화군에서 의미있게 낮았다. 혈중 크레아티닌 농도는 두 군 간에 유의한 차이가 없었으나, 혈중 요산 농도는 악화군에서 의미있게 높았다. 요중 크레아티닌 농도 및 크레아티닌 청소율은 악화군에서 의미있게 낮았다. ALT, AST 및 적혈구용적율은 두 군 간에 유의한 차이가 없었으나, LDH 농도는 악화군에서 의미있게 높았다. 결론: 체계적 평가에서 경증 전자간증으로 판명되더라도 혈중 알부민, 요중 요산 농도 및 크레아티닌 청소율이 낮고, 혈중 요산 농도 및 LDH가 높게 나오면 태반 기능장애가 악화될 가능성이 높으므로 분만 전까지 철저한 산전 검사와 분만 중에 각별한 주의가 필요할 것으로 사료된다. Objectives: To compare the systematic evaluations in hospitalization of mildly preeclamptic women whose placental dysfunction was exacerbated with those whose placental dysfunction was not exacerbated. Methods: There were seventy six mildly preeclamptic women after systematic evaluations in hospitalization, in which twenty eight women showed exacerbated placental dysfunction. Outcome markers considered suggestive of exacerbation of placental dysfunction included oligohydramnios (amniotic fluid index less than 5 cm), clinical diagnosis of intrapartum fetal distress and progression to severe preeclampsia. A clinical diagnosis of fetal distress was made when the monitoring revealed recurrent late, persistent tachycardia with loss of variability or prolonged decelerations. Results: There were no significant differences in the amount of 24 hours urine protein, the concentrations in serum protein, creatinine, aspartate transaminase and alanine transaminase and hematocrit. But in exacerbated group, the concentrations of serum albumin and urine creatinine and creatinine clearance were significantly lower and the concentrations of serum uric acid and lactate dehydrogenase were significantly higher than not exacerbated group. Conclusion: In mildly preeclamptic women diagnosed after systematic evaluation in hospitalization, we must give attention to those whose concentrations of serum albumin and urine creatinine and creatinine clearance are low and concentrations of serum uric acid and lactate dehydrogenase are high.

외음부 병변이 없는 산모에서 발생한 제 2형 단순 포진 바이러스 뇌염 1예

강윤단 ( Yun Dan Kang ) 대한주산의학회 2015 Perinatology Vol.26 No.2

급성 중추 신경계 감염을 일으키는데 바이러스 중 단순 포진 바이러스는 급성 뇌염을 일으키는 가장 흔한 원인이다. 단순 포진 바이러스 1형과 2형은 각각 다른 신경학적 증상을 나타내는데, 특히 2형의 경우는 일반적으로 면역력에 이상이 없는 경우 자기 제한적 무균 수막염(self-limiting aseptic meningitis like illness)의 형태로 나타나며 재발성 뇌수막염을 보이기도 한다. 임신 중 단순 포진 바이러스 2형 뇌염은 매우 드문 질환으로, 이로 인해 진단이 늦어져 치료가 지연되는 경우 산모 및 신생아 예후에 악영향을 미칠 수 있다. 뇌척수액 바이러스 중합효소 연쇄 반응 검사를 통해 진단을 하며 acyclovir로 조기 치료가 매우 중요하다. 이에 저자들은 외음부 피부 병변이 없는 산모에서 충수돌기 절제술 후 발생한 단순 포진 바이러스 2형 뇌염을 조기에 acyclovir로 치료하여 질식 분만 후 신경학적 후유증을 남기지 않은 증례를 보고한다. Herpes simplex encephalitis is the most common form of encephalitis, but herpes simplex virus type 2 (HSV-2) encephalitis is very rare during pregnancy. In immunocompetent patients, HSV-2 encephalitis is usually mild. With early diagnosis and prompt initiation of antiviral treatment, as well as enhanced neurocritical care, a favorable outcome can be expected in both mother and neonate. We report a rare case of maternal HSV-2 encephalitis diagnosed by polymerase chain reaction following appendectomy. The woman had no symptomatic genital lesion, and the infant was not infected.

산전 부신피질호르몬 일회 투여 후 분만까지 기간이 조산아 예후에 미치는 영향

강윤단 ( Yun Dan Kang ),박만철 ( Man Chul Park ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.1

목적: 산전에 투여한 부신피질호르몬 일회 요법의 장점이 시간이 지남에 따라 어떻게 변하는지 알아보기 위함이다. 연구 방법: 본원 산부인과에서 산전 부신피질호르몬 일회 요법의 전 과정을 투여받은 후, 임신 26주 이후 및 35주 이내에 분만하였던 신생아 203명을 대상으로 신생아 입원 차트를 후향적으로 검토하였다. 첫 부신피질호르몬 투여부터 분만까지의 기간이 48시간 후 7일 이내군 (78예), 8일에서 14일 사이군 (65예) 및 15일 이상군 (60예)으로 구분하여 신생아 이환율을 비교하였다. 결과: 각 군의 임상적 특징 및 산전 부신피질호르몬 투여 적응증의 분포, 분만을 시도하게 된 적응증의 분포에 차이는 없었다. 24시간 이상 인공호흡기 부착률은 7일 이내군이 32.1% (25/78)로 8~14일군의 50.8% (33/65)(P=0.023) 및 15일 이상군의 51.7% (31/60)(P=0.02)보다 의미 있게 낮았으며, 8~14일군과 15일 이상군 사이에 유의한 차이는 없었다. 그러나 평균 인공호흡기 부착 기간, 표면활성제 투여율, 임신 연령 36주에 산소의존율, 분만 후 28일에 산소의존율, 뇌실내출혈 발생 빈도, 괴사소장 대장염 발생 빈도, 패혈증 발생 빈도 및 입원 기간 등에서는 차이가 없었다. 모든 군에서 뇌측실부 백질 연화증과 신생아 사망은 없었다. 결론: 산전 부신피질호르몬 투여 후 7일이 지나면 24시간 이상 인공호흡기 부착률이 증가할 수 있으나, 다른 모든 이환율엔 차이가 없었다. Objective: To determine whether the beneficial effects of a single course of antenatal corticosteroids for neonatal morbidity change with time Methods: A retrospective chart review was performed of women who received a single complete course of antenatal corticosteroid and delivered a viable singleton infant between 26 and 35 weeks of gestation. Patients were divided into 1 of 3 groups on the basis of the interval from first corticosteroid dose to delivery (48 hr~7 days, 8~14 days and ≥15 days). Neonatal morbidities were compared between each groups. Results: Two hundred three pregnancies were included, of which 78 women delivered at 48 hr-7 days, 65 women delivered at 8~14 days and 60 women delivered at ≥15 days. The 3 groups were similar in clinical characteristics and indications for antenatal steroids and delivery. Neonates delivered within 7 days had a lower incidence of receiving ventilatory support for more than 24 hours than 8~14 days group (32.1% vs 50.8%, P=0.023) and ≥15 days group (32.1% vs 51.7%, P=0.02). But there were no significant differences between the groups in ventilator days, surfactant use, oxygen dependency at 36 weeks of gestation, oxygen dependency at 28 days after delivery, intraventricular hemorrhage, necrotizing enterocolitis, sepsis and length of hospital days. There were no periventricular leukomalacia and neonatal death in all groups. Conclusion: Neonates delivered more than 7 days after first corticosteroid dose needed more short-term ventilatory support, but there were no differences in other neonatal outcomes.

고강도 집속 초음파(HIFU) 후 임신 제 2 삼분기에 발생한자연 자궁파열 1예

강윤단 ( Yun Dan Kang ),박충학 ( Choong Hak Park ) 대한주산의학회 2015 Perinatology Vol.26 No.4

자궁파열은 드물게 발생하지만, 치명적인 질환으로 모성과 태아의 이환율 및 사망률이 매우 높은 산과적 응급질환 중 하나이다. 가임기 여성에서 최근 자궁근종의 치료로 고강도 집속 초음파가 시행되고 있으나 시술 후 임신을 했을 때 자궁파열을 초래할 수 있지만 임신의 예후 및 시술에 관한 적응증, 지침은 부족한 상황이다. 이에 저자들은 자궁근종의 치료를 위해 고강도 집속 초음파 후 임신 25주에 자궁파열이 된 1예를 경험하였기에 문헌고찰과 함께 이를 보고하는 바이다. Uterine rupture during pregnancy does not occur frequently, but is associated with high rates of maternal and perinatal morbidity and mortality. As a non-invasive and conservative approach, high-intensity focused ultrasound (HIFU) has received attention from both gynecologists and patients for the treatment of fibroids, especially women who wish to preserve uterus. However, there are not enough studies about complication and prognosis related pregnancy after HIFU. We present a case of uterine rupture that occurred in second trimester who had been HIFU 3months before pregnancy.

갑상선 기능항진증 산모에서 제왕절개 분만후 진단된 비대칭성 울음 안면 1 예

강윤단 ( Kang Yun Dan ),송언호 ( Song Eon Ho ),박진완 ( Park Jin Wan ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.2

Asymmetric crying face is caused by unilateral weakness of the depressor muscles of lower lip, which is most obvious during crying and is associated with other congenital anomalies, especially congenital heart disease. We experienced a case of asymmetric crying face after cesarean delivery in mother with hyperthyroidism and present it with a brief review of literature.