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Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.
( Jun Pei Zhou ),( Qian Wu ),( Rui Zhang ),( Yu Ying Yang ),( Xiang Hua Tang ),( Jun Jun Li ),( Jun Mei Ding ),( Yan Yan Dong ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation time 72 h; 30℃; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60℃ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at 30℃ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.
( Jun Mei Ding ),( Ting Ting Yu ),( Lian Ming Liang ),( Zhen Rong Xie ),( Yun Juan Yang ),( Jun Pei Zhou ),( Bo Xu ),( Jun Jun Li ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11
The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around 50°C at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an α/β-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.
Guo-Jun Yu,Shao-Jie Zhu,Cheng-Bin Du,Ling-Yun Wang,Jun-Chi Huang 한국자기학회 2023 Journal of Magnetics Vol.28 No.3
This paper studies magnetic field control and velocity-activated magnetorheological shear thickening fluid (MR-STF). High-concentration STF is composed of nano-sized silica particles suspended in a solvent polyethylene glycol (PEG), and then micron-sized carbonyl iron particles of different mass fractions are added to the STF to manufacture different MR-STF. The rheometer is used to study the viscoelasticity of all four samples. The correlation between dynamic behavior and shear rate, angular frequency, and external magnetic field is studied and discussed. In the lower angular frequency range, the loss modulus is slightly larger than the storage modulus, and MR-STF behaves as a viscoelastic state. After the critical angular frequency, the storage modulus decreases sharply, well below the loss modulus. MR-STF appears in a viscous state and a liquid state. With the start of external field excitation, MR-STF is more inclined to MRF. Finally, the apparent viscosity and shear rate of MR-STF are fitted. The results show that with the increase of magnetic induction strength, the plastic viscosity coefficient of MR fluid increases, the flow characteristic index decreases, and the shear thinning effect becomes more significant.
Ding, Xiao-Yu,Xu, Qiu,Zhu, Xiao-yong,Luo, Lai-Ma,Huang, Jian-Jun,Yu, Bin,Gao, Xiang,Li, Jian-Gang,Wu, Yu-Cheng Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.12
Oxide dispersion-strengthened materials W-1wt%Pr<sub>2</sub>O<sub>3</sub> and W-1wt%La<sub>2</sub>O<sub>3</sub> were synthesized by wet chemical method and spark plasma sintering. The field emission scanning electron microscopy (FE-SEM) analysis, XRD and Vickers microhardness measurements were conducted to characterize the samples. The irradiations were carried out with a 5 keV helium ion beam to fluences up to 5.0 × 10<sup>21</sup> ions/m<sup>2</sup> under 600 ℃ using the low-energy ion irradiation system. Transmission electron microscopy (TEM) study was performed to investigate the microstructural evolution in W-1wt%Pr<sub>2</sub>O<sub>3</sub> and W-1wt%La<sub>2</sub>O<sub>3</sub>. At 1.0 × 10<sup>20</sup> He<sup>+</sup>/m<sup>2</sup>, the average loops size of the W-1wt%Pr<sub>2</sub>O<sub>3</sub> was 4.3 nm, much lower than W-1wt% La<sub>2</sub>O<sub>3</sub> of 8.5 nm. However, helium bubbles were not observed throughout in both doped W materials. The effects of pre-irradiation with 1.0 × 10<sup>21</sup> He<sup>+</sup>/m<sup>2</sup> on trapping of injected deuterium in doped W was studied by thermal desorption spectrometry (TDS) technique using quadrupole mass spectrometer. Compared with the samples without He<sup>+</sup> pre-irradiation, deuterium (D) retention of doped W materials increased after He<sup>+</sup> irradiation, whose retention was unsaturated at the damage level of 1.0 × 10<sup>22</sup>D<sub>2</sub><sup>+</sup>/m<sup>2</sup>. The present results implied that irradiation effect of He<sup>+</sup> ions must be taken into account to evaluate the deuterium retention in fusion material applications.
( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
Social Network Partition Method based on Concentricity-Controllable Routing
Huang Jian-Heng,Yu Jun-Qi,Zhao Min-Hua 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4
In allusion to issues that partitioning algorithm of traditional social network community is generally lacking in comprehensive consideration of node and link attributes as well as full expression of model and mechanism using attributes of node and link. This paper presents a social network community partitioning algorithm fusing node and link attributes. The algorithm has blended similarity of node properties, link weights between nodes and link attribute information, making definition of the similar weights, and on this basis, social networking community division is realized combined with condensation algorithms. Experiments show that the algorithm has remarkable effects on community partition with obvious attributes in social network.
H-mode experimental integrated modeling of impurity injection to control divertor heat flux on HL-2A
Yu Peng,Huang Qianhong,Zhong Yijun,Wu Ting,Wang Jun,Wang Zhanhui,Gong Xue-yu 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.82 No.8
Based on the METIS integrated modeling platform, combined with the experimental data of the #34512 shot detached divertor operation on the HL-2A, this paper selects suitable physical models, and compares and analyzes the integrated modeling results with the experimental results. The modeling results are in good agreement with the experimental results. On this basis, the reason for the decrease of the peak heat fux on the divertor targets is investigated using the METIS simulator. The results show that the two factors of entering the H-mode and the radiation enhancement efect generated by impurity injection work together to reduce the heat fow on the targets. Finally, the diferent efects of N, Ar and Ne impurity gases on the heat fux reduction of the targets are simulated under the condition that the plasma line-average efective charge (Zef) is kept unchanged. The results show that N and Ar are both better than Ne in controlling the heat fow on the targets, and N has the best compatibility in the core, which can not only reduce the boundary heat fow but also maintain the core confnement performance.
Research on Gateway Deployment Solving Model Applied in the Wireless Mesh Network
HUANG Jian-Heng,YU Jun-Qi,ZHAO Min-Hua 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3
In order to solve gateway deployment of geometry K center in the wireless network and improve the service quality of network, this paper proposes the gateway deployment solving model and algorithm. This paper transforms center problem of the geometric K into center problem of node K and solves problem. This paper proposes a gateway node alternative selection algorithm combination of network topology properties and maximum cover area related theory, and according to the relevant features select an alternative area, each alternative area abstract for a virtual node and is inserted into the original network topology structure, thus forming the new network topology. Solving new problem of the network topology and looking for the optimal deployment scheme use the improved genetic algorithm. The experimental results show that the model and algorithm proposed in this paper can well solve the wireless network gateway deployment problem of geometric K center and improve the network service quality.