Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

연구논문 : 대학생들의 인터넷 중독에 영향을 미치는 요인에 대한 연구

권미영 ( Mi Young Kwon ),황지영 ( Ji Young Hwang ),정혜윤 ( Hye Yoon Jung ),송시형 ( Shi Hyeong Song ) 극동대학교 사회복지연구소 2013 글로벌사회복지연구 Vol.3 No.2

본 논문의 목적은 대학생의 인터넷 중독에 영향을 주는 요인에 대한 분석을 통해 대학생의 정신건강을 위한 공적프로그램 도입에 대한 함의를 제공하는 것이다. 분석자료는 을지대학교 강남 을지 병원 중독연구소에서 대학생 453명을 대상으로한 설문지이다. 설문조사에 사용된 설문지는 인터넷 중독을 측정하는 인터넷 중독 척도(Young척도), 불안을 측정하는 불안척도(STAI)와 우울을 측정하는 Beck의 우울척도(BDI)로 구성되었다. 분석결과 첫째, 연령 및 학년은 인터넷 중독에 유의미한 영향을 미쳤다. 둘째, 나이가 어릴수록, 학년이 낮을수록 불안과 우울에 빠지기 쉬웠다. 셋째, 무엇보다 불안이 대학생의 인터넷 중독에 가장 큰 영향을 미치는 요인이었다. The purpose of this research to suggest implications on introduction of public-program for college students` mental health through analysing the factors which impact on internet addiction of college students. The analysing data is questionnaire intended to 453 college students in Kang- Nam Eulji hospital addiction institute. The questionnaire consisted of internet addiction scale (Young-scale), anxiety scale (STAI) and depression scale (BDI). This study reveals three main findings. Firstly, age and grade have an effect on internet addiction. Second, the younger, the lower grade, they are likely to be depressed and anxiety. Third, most of all, the anxiety has the most effect on internet addiction of college students.

배우자의 지지와 신생아에 대한 지각에 관한 연구

윤영미 瑞逸大學 1999 論文集 Vol.17 No.-

테니스 포핸드시 밀어치기와 때려치기 타법의 운동학적 분석

윤양진,정미라,서국웅,이훈식,황명숙,강영택,윤길환 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

This study consists in clarifying the meaning of various angles and velocity, and two types of the stroke of a pushing shot and hitting shot through the three-dimensional image analyses among the factors of exercise. To attain the purpose of such studies, the result of experiment and analysis for each of three athletes of boys and girls in high school and three persons in general came to the conclusion as follows; 1. The last motion of a group of A having a command of a follow shot showed greatly, compared with two groups of B and C, and this showed that a group of A took a rectilinear motion greatly to gain power and two group of B and C took a rotary motion greatly to gain speed. 2. A group of A having a command of a pushing shot move body weight forward from behind and give the first consideration to a rectilinear motion, and two groups of B and C having a command of the stroke of hitting shot used the turning of the shoulder and the waist and gave much weight to a rotary motion than a rectilinear one. 3. The position of the hitting point of a group of A having a command of a pushing shot turned out to be the waist, and that of the hitting point of two groups of B and C, the chest, and namely this showed that the position of the hitting point was decided according to the form of the stroke and pictures of various kinds. 4. In impacting, the speed decreased, for the power was controled to keep an accurate timing of stroke just before impacting

대학생을 위한 예비 부모 교육 프로그램 개발을 위한 연구

윤기영,전효숙,박상임,이미숙,이석란,박수옥 서원대학교 학생생활연구소 1998 학생생활연구 Vol.16 No.-

The purpose of this study are to examine reference materials, existing lecture analysis, investigate how student response about new lecture model and to project the basic direction of pre-parent education program. The result of this study are as follows ; <Lecture Model : Parent and Child> Sequence Theme 1 Introduction, Positive Self-Concept 2 Love and Sex 3 Friends versus Lovers 4 Marriage 5 Parenting and Parent's Role 6 Pregnancy and Embriology 7 Brith 8 Middle Term Exam 9 Parent's Role for Infant and Toddler 10 Parent's Role for Child 11 Parent's Role for Youth 12 Society and Environment for Child Care 13 Communication for Parent-Child 14 Problem solving method for Child Care 15 Parent in Future 16 Final Exam

학교 통일교육지침에 입각한 유치원의 통일교육 구현 방향 모색

윤기영,이미숙 서원대학교 교육연구소 1995 敎育發展 Vol.1995 No.14

The purpose of this study was to investigate how the kindergarten education for reunification in Korea should be, based on the 「Guides for School Teacher」published by Education Ministry. Necessity, appropriateness, and possibility of this education were considered. The results of this study were as follows; Education for reunification in Korea must be done in kindergarten as well as inordinary school because reunification of Korea is essential for us. Also many concepts and characters related with this education are formed and developed in early childhood. And it is one of reasons that early childhood deucation is very important as well as known. So, for successful this education systematic programs should be developed, and education about reunification should be reinforced for kindergarten teacher. Howerer, for the time being, since new programs are not yet developed it is desirable that the current programs such as 「human Education」, 「Democratic Citizenship Education」,「Education for peace」 be used instead to achieve this goal.

학부모기로의 전환에 관한 연구

윤기영,이미경,박민자 서원대학교 교육연구소 2003 敎育發展 Vol.22 No.1

Parents experience a qualitative change in terms of relationship with their hildren when they start to attend the first educational institute. Parents' role as a 'Hakbumo', whose children are attending school, could be quite different from parents' general role at home. Therefore, it might be needed to investigate the 'Hakbumohood' transition process for appropriate parents development and home-institute relationship After the investigation of literature, in-depth interview and discussion was conducted with 7 early childhood education center 'hakbumo', 2 kindergarten teachers, 1 professor. The result of this study, 1) Most parents take they become a 'Hakbumo' as a matter of course. 2) They feel anxiety about their children's adaptation to new circumstances. 3) They feel variety feelings during the 'Hakbumohood' transition. 4) Their feelings and concern are different between pre attending institute and post attending 5) They were influenced wholeness environment So, it might be concluded that developing a individualized guidance and support program for each new ‘Hakbumo’

Ocular MALT Lymphoma : Report of Two Cases

윤영묵,장효찬,김동석,김상원,박미옥,박재복 대구효성가톨릭대학교 1998 연구논문집 Vol.57 No.2

Lymphoma arising in mucosa-associated lymphoid tissue(MALT) is extranodal marginal zone lymphoma displaying a proliferation of follicle marginal zone-type cells(centrocyte-like cells or monocytoid B-cells), often with surrounding reactive germinal centers and lympoepithelial lesions. This typically expresses pan-B-cell markers and monotypic immunoglobulin but not CD5 or CD10. Ocular MALT consists of the lacrimal gland, lcular soft tissue, orbit, conjunctiva and cornea. Lymphoma arising in thses sites is of very rare occurrence in up to 1.5% of all patients with non-Hodgkin's lymphoma. Herein we report two cases of ocular MALT lymphoma which was very rare in the dermatologic field. Case 1, 73-year-old man, developed the right upper conjunctival swelling measuring about 2.0×2.5cm for about 1 year. Histologically, the excised lesion showed follicular centers and infiltration of centrocyte-like cells of B-cell lineage in the stroma but no lymphoepithelial lesions, consistent with low-grade B-cell lymphoma. Case 2, a 66-year-old female, had a rapid course of about 1.2×1.0cm-sized, soft, wellow mass arising from lacrimal gland of the right upper eyelid for about 1 month. Hostologically, the lesion showed diffuse patterend features admixed with centrocyte-like cells and large cells such as centroblast, compatible with high-grade lymphoma. Case 2 received combined chemotherapy of m-BACOD, with a somewhat improvement.

예비교사를 위한 '교사의 인간관계'강좌 수업계획 시안

윤기영,이미숙 서원대학교 학생생활연구소 2000 학생생활연구 Vol.18 No.-