위암에서의 중합효소 연쇄반응에 의한 Epstein - Barr 바이러스의 검출과 LMP1 , p53 , CD44 의 발현

이명아(Myung Ah Lee),홍영선(Young Seon Hong),강진형(Jin Hyung Kang),이경식(Kyung Shik Lee),유진영(Jin Young Yoo),이교영(Kyo Young Lee) 대한내과학회 2002 대한내과학회지 Vol.63 No.3

N/A Background : Epstein-Barr virus (EBV) is associated with various lymphoproliferative disorders and nasopharyngeal carcinoma. Recently some of the tumor cells of gastric cancer were observed to contain EBV sequence. We detected EBV using PCR to determine the frequency of EBV associated gastric cancer, which is the most common cancer in Korea. We also performed immunohistochemical staining for the latent membrane protein (LMP1), p53 and CD44 to investigate the possible mechanism in EBV-associated gastric cancer. Methods : Eighty-seven formalin-fixed and paraffin-embedded blocks (40 gastric adenocarcinomas, 34 adjacent normal tissues, 13 metastatic lymph nodes) from 40 surgically resected gastric specimens were studied. All patients were diagnosed as having gastric cancer at the Kang-Nam St. Mary's Hospital during the period of April 1995 to April 1997. DNA was extracted from each paraffin block and then we performed PCR and immunohistochemical staining for the LMP1, p53 and CD44. We reviewed the patients' charts for clinical information. Results : EBV was detected in 4 cases (10%) by EBV-PCR among the 40 patients. It was also detected in a metastatic lymph node in 1 patient. The immunohistochemical staining for the LMP1, p53 and CD44 was negative in all the EBV-positive cancer patients. Of the patients having these cancers, 2 had a poorly differentiated adenocarcinoma with a lymphoepithelioma-like morphology. Conclusion : The frequency of EBV-associated gastric cancer may be about 10% in Korea. Considering the negative result of the immunohistochemical staining for the LMP1, p53 and CD44, EBV-associated gastric cancer seems to have a different mechanism of tumorigenesis from ordinary gastric cancer or other EBV-associated cancers. This must be determined by further large scale studies. (Korean J Med 63:261-266, 2002)

Amino-Functionalized Mesoporous Silica Particles for Ocular Delivery of Brimonidine

Kim, Se-Na,Ko, Song Ah,Park, Chun Gwon,Lee, Seung Ho,Huh, Beom Kang,Park, Yoh Han,Kim, Young Kook,Ha, Ahnul,Park, Ki Ho,Choy, Young Bin American Chemical Society 2018 MOLECULAR PHARMACEUTICS Vol.15 No.8

<P>To treat glaucoma, conventional eye drops are often prescribed. However, the eye drops have limited effectiveness as a result of low drug bioavailability due to their rapid clearance from the preocular space. To resolve this, we proposed amino-functionalized mesoporous silica (AMS) particles as delivery carriers of the glaucoma drug, brimonidine. Because of the presence of mesopores, brimonidine (BMD) could be encapsulated in the AMS with a loading amount of 41.73 μg/mg (i.e., drug loading capacity of about 4.17%) to give the BMD-AMS, which could release the drug in a sustained manner over 8 h. BMD-AMS was also shown to be mucoadhesive due to the presence of both hydroxyl and amino groups in the surface, allowing for formation of hydrogen bonds and an ionic complex with the mucin, respectively. Therefore, when topically administered to rabbit eyes in vivo, BMD-AMS could reside in the preocular space for up to 12 h because of its adherence to the mucous layer. To assess in vivo efficacy, we examined the variance in intraocular pressure (IOP) and brimonidine concentration in the aqueous humor (AH) after applying BMD-AMS to the eye, which was compared with that induced by Alphagan P, the marketed brimonidine eye drops. For BMD-AMS, the duration in the decrease in IOP and the area under the drug concentration in the AH-time curve (AUC) were 12 h and 2.68 μg·h/mL, respectively, which were about twice as large as those obtained with Alphagan P; this finding indicated enhanced ocular bioavailability of brimonidine with BMD-AMS.</P> [FIG OMISSION]</BR>

In vivo study for the hemostatic efficacy and foreign body reaction of a new powder-type polysaccharide hemostatic agent

Yoonhyeong Byun,Eun Jin Kim,Areum Lee,Young-Ah Suh,Hee Ju Sohn,Jung Min Lee,Jae Seung Kang,Yoo Jin Choi,Youngmin Han,Hongbeom Kim,Wooil Kwon,Jin-Young Jang 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.2

Purpose: Various hemostatic agents have been introduced in therapy as postoperative bleeding is a poor prognostic factor for postoperative outcomes. These products can be divided into those that directly promote the hemostatic cascade and those that physically form a barrier by absorbing blood. The latter, powder-type hemostatic agents have the advantages of being inexpensive and more absorbable with less foreign body reactions (FBRs) and are applicable to a relatively wide area. This study was conducted to verify the safety and efficacy of a newly invented polysaccharide product (OOZFIX, Theracion Biomedical), which improves blood absorption and hemostatic effects. Methods: Two separate animal experiments were performed. The first evaluated FBRs histologically at 3 days, 2 weeks, and 4 weeks, after implantation of OOZFIX in rats, and the second compared hemostatic performance of OOZFIX and Arista AH (Bard) in the porcine liver punch biopsy model. Results: We found minimal FBRs in the 3-day group and no reactions in both the 2-week and 4-week groups after implantation of hemostatic agents. The time to hemostasis of OOZFIX was not significantly different from that of Arista AH (median [interquartile range]: 9 [6–10] minutes vs. 8 [6–10] minutes, respectively; P = 0.522). When comparing the serial bleeding grade tendency, there was no statistical difference between OOZFIX and Arista AH (P = 0.656). Conclusion: OOZFIX caused a minimal FBR that disappeared within 2 weeks in vivo, and its hemostatic performance was comparable with that of an existing agent, Arista AH. Further clinical studies are required in the future.

무혈혈당측정

강나루,우영아,김효진 동덕여자대학교 종합약학연구소 2001 동덕약학연구지 Vol.5 No.-

The purpose of this study is to develop a noninvasive blood glucose measurement method by using near infrared spectroscopy (NIRS). Reflectance NIR spectra of different parts of human body (finger tip, earlobe, and inner lip) were Recoreded by using a fiber optic probe. The spectra were collected over the spectral range 1100-1700nm. Partial least squares regression (PLS) was applied for the calibration and validation for the determination of blood glucose. The calibration with the use of PLS model predicted for validation set glucose concentration with a standard error of prediction (SEP) of 33㎎/㎗.

전국 단위 감시체계의 현황과 전망

강성규,안연순,지영구,김형옥,하미나,권호장,백남종,김성아,홍윤철,김재용,강대희,정혜선,하은희 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

석유화학공장 근로자에서 발생한 재생불량성 빈혈 1례

박영만,임영,강성규,김지홍,이종욱,최용휴,김경아 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.2

Aplastic anemia is characterized by pancytopenia with hypocellular bone marrow. Fifty percent of the cases are idiopathic and the rest are caused by various agents including drugs, chemicals, radiation and viruses. It is difficult to link specific etiologic agents, especially chemicals to the development of aplastic anemia because multiple or unknown exposures may be involved in. Benzene, a common industrial chemical and a component of gasoline, may lead progressively to pancytopenia, aplastic anemia and leukemia when exposed. A petrochemical worker with aplastic anemia was referred to our hospital to evaluate a relationship between the job history and the disease. He worked in the petrochemical plant for 21 years and was exposed to low-level benzene. There was not anyother etiologic agent except benzene and this is the case report of aplastic anemia which possibly due to benzene exposure.

가설설정에서 그럴듯한 점, 발전시킬 점, 우려되는 점으로 질문하는 활동으로 화학 실험을 경험한 대학생들의 창의적 문제 해결력

손아영,강순희 이화여자대학교 교과교육연구소 2014 교과교육학연구 Vol.18 No.4

본 연구에서는 대학교1학년 학생들이 수강하는 일반 화학 실험에서 가설설정 하기를 그럴 듯 한점, 발전시킬점, 우려되는 점으로 질문하고 쓰게 하는 PPC 질문 기법으로 활동하는 한 학기용 실험 교수 전략을 개발하였다. 본 연구에서 개발한 한 학기용 총 10개 주제의 실험들을 적용한 후에, 본 실험 교수 전략을 경험한 실험반과 그렇지 아니한 비교반 학생들의 발산적 사고력인 협의의 창의적 사고력의 변화를 분석하였다. 그리고 탐구 기능 측면으로 비판적 사고력의 변화도 분석하였다. 적용한 실험 교수 전략을 한 학기 동안 경험한 학생들의 협의의 창의적 사고력이 통계적으로 유의미하게 향상된다(p <.05)는 결론을 얻을 수 있었다. 또한 협의의 창의적 사고력의 3개 하위 요소들인 유창성, 융통성 그리고 독창성에서 통계적으로 모두 유의미하게 향상되었다(p <.05). 그리고 이 학생들의 문제 해결 측면인 탐구 기능에 대한 비판적 사고력도 통계적으로 유의미하게 향상되었다(p <.05). 또한 탐구 기능에 대한 비판적 사고력인 5개 하위 요소들 중에서 가설 설정 능력에 대한 비판적 사고력은 통계적으로 유의미하게 향상되었으나(p <.05), 문제인식능력, 변인통제능력, 자료 해석 및 자료변환능력, 결론 도출 및 일반화 능력에 대한 비판적 사고력은통계적으로 유의미하게 향상되지 않았다(p >.05). 이는 대학교 실험 수업에서 대학생들의 협의의 창의적 사고력을 신장시키는 실험 교수 전략을 제공하기 위한 기초 자료로 활용 될 수 있을 것이다. ‘This study examined the efficacy of ’Positives-Possibilities-Concerns’ question activities about making hypotheses, for the purpose of enhancing creative problem solving thinking of pre-service science teachers in college. Ten ‘PPC’ question activities were presented as teaching materials to students over one semester. The experimental group showed a statistically meaningful improvement in creative thinking skills(p<.05). In addition, the three sub-elements of creative thinking skills, i.e., fluency, flexibility and originality, all showed statistically meaningful improvement(p<.05). Also, there was significant statistical improvement in critical thinking skill with respect to the inquiry thinking skill(p<.05). The sub-elements of critical thinking skills and making hypotheses showed a statistically meaningful improvement (p<.05), but there were no significant statistical improvements in recognition of problems, control of variables, transformation and nterpretation of data, and making conclusions and generalizations(p>.05). This result may become the basis for the enhancement of creative problem solving thiunking in the general chemistry laboratory in college for the future.

모형식품의 해동방법에 따른 물성변화

이재학,박영덕,강현아,장규섭 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.2

Optimal thawing conditions of frozen model foods containing protein and starch were investigated at various thawing conditions such as room temperature, hot air, and microwave heating. Hardness of the frozen model foods was getting higher as the water content increased. Thawing rates at room temperature, hot-air heating at 50℃, and microwave heating were 0.02Kg/min, 0.08Kg/min. 0.01 Kg/min. respectively. Final thawing time was as follows; control 60min, 5% sucrose: 50min, 10% sucrose: 30 min, 5% NaCl: 30 min. Total drip loss was as follows; room temperature thawing: 22.5%, 200W microwave thawing 1.3%, and 50℃ hot air thawing nearly negligible.

우리나라 지역 직업성질환 감시체계의 현황과 전망

임종한,장성실,김성아,문재동,채창호,홍윤철,김수영,김진석,김영욱,한상환,이혜숙,원종욱,송동빈,하은희,강성규 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

기존의 특수건강진단과 작업환경측정을 통한 직업병 관리가 진폐증, 소음성난청 등의 소수 특정질환에 국한되고 실제 직업병 발생 규모 파악이나 신종 직업병의 발견에 한계를 보인다는 사실은 산업의학전문가들 사이에서도 공감을 이루고 있다. 미국과 영국 등에서의 직업성질환 감시체계에 대한 경험은 우리 나라의 직업성질환 감시체계 구축에도 새로운 자극제가 되면서, 1998년이후 인천, 대전, 여천, 구미, 부울경 지역에서 지역 직업성질환감시체계를 산업보건관리의 중요한 시스템으로 구축하려는 노력이 확산되고 있다. 새로이 구축되어지는 이들 지역 직업성질환 감시체계는 감시하고자 하는 대상질환, 활용 가능한 인적자원 및 자료원, 지역 의료체계의 특수성 등에 따라서 목적과 방법을 달리하면서 독특한 형태로 발전을 하고 있다. 각 지역단위 감시체계들이 그 상황에 맞게 독특한 목적과 전략들을 발전 시키면서도, 향후 발전할 국가적인 차원의 직업성질환 감시체계 구축을 위하여 직업성질환 감시의 기본 전략 등을 공유하는 등의 노력이 필요하다. 환례 정의 및 기본적인 등록 서식의 공유, 직업성질환 감시 자료원의 발굴, 공동의 정보 네트워크 및 직업성질환 감시 데이터베이스 구축 등 직업성질환 감시활동을 지원하기 위한 여러 기초 인프라 구축에 힘을 모아야 할 것이다. 우리 나라에서 직업성질환 감시체계를 성공적으로 구축하기 위해서는 수집된 자료의 질 관리를 위한 직업성질환 감시의 원칙 제정과 감시 전략의 공유 등이 필요하며, 전국적인 직업성질환 감시체계의 하부구조라고 할 수 있는 지역감시체계의 기초 토대 마련과 강화작업이 절실하게 필요하다.

암치료에 있어서 체질과 항암효과에 관한 한의학적 연구

성현제,신현규,박갑주,강봉주,은영아,김은해,정세영 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

As one of the chemical and experimental study on cancer treatment in Korea oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows : About Clinical Aspects of the Oriental Medical Cancer Treatment - (1) Clinical experience is that 420 Oriental medical hospital were surveyed. Number of cancer patient treated by one oriental medical doctor was under 10(about 60%) or from 10 to 20(about 30%). (2) Diagnostic Procedure is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study - to evaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical presciptions on stomach cancer cell, SRB assay was carried out. (1) The IC50 value of water extract of Samsoum was 19㎍/㎖ and that of methanol extract was 15㎍/㎖, so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The IC50 values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were 10㎍/㎖, 19㎍/㎖ and 12.5㎍/㎖ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Draba Semen have a strong direct cytototoxicity. (3) The IC50 value of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about 40㎍/㎖.