Squalene Adjuvant를 이용한 단백질 항원의 세포성 면역반응 유도

도현주,김성열,안종성,하영주,이승찬,오재택,장명호,정홍석,문홍모,박해준 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

To investigate the role of adjuvant for cytotoxic T lymphocytes (CTL) induction, squalene-based adjuvant was studied its capability eliciting to the induction of cellular immunity as well as humoral immunity to exogeneous proteins. Ovalbumin (OVA) was used as model proteins. It was demonstrated that antigen formulation consisted of metabolizable oil squalene mixed with Tween 80 and pluronic L121 (S/TJ121) could induced the antigen specific CTL responses and antibodies irrespective of immunization routes in mice. We also demonstrated that this antigen formulation was a inducer of CD8+, major histocompatibility complex(MHC) class I-restricted, and antigen-specific C;1'Ls. These data suggest that the squalene-based emulsion system is a potent adjuvant inducing cellular immunity as well as humoral immunity.

자발성 뇌지주막하 출혈 환자에서 최초 뇌실 크기의 임상적 의의

신일영,윤일규,윤석만,도재원,이경석,배학근,최순관,변박장 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

The purpose of this study is to invesetigate the clinical significance of initial enlargement of ventricle in the patients who had aneurysmal SAH. Of the total 821 patients with aneursmal SAH who were registerd in SAH data bank system between 1989-1999, 307 patients who had been already measured in the size of ventricle at the time of admission, were studied retrospectively. The size of ventricle was calculated by bifrontal index. Normal size of ventricle was defined as 30% or less, mild enlargement was defined as 31 to 39%, and moderate enlargement of vertricle was defined as more than 39% in bifrontal index. The sizes of ventricle using bifrontal index were analysed according to the age, sex, blood pressure, consciousness at admission, medical history of hypertension, location of ruptured aneurysm, Fisher's grade on CT(computerized tomography) scan at the time of admission, cerebral vasospasm, intraventricular hemorrhage (IVH), performance of external ventricular drainage (EVD), and prognosis at discherge. Chi-square test and ANONA were used in the statistical evaluation. The results were as follows : 1) Of the 307 patients, 235(76.5%) had a mild to moderate enlargement of ventricle at the time of admission (more than 30% in bifrontal index). 2) The size of ventricle was enlarged according to the increase of age (p<0.05). 3) In the patients who had ruptured aneurysm on anterior cerebral artery, the size of ventricle significantly increased and showed a higher incidence in mild enlargement compared with moderate enlargement of ventricle (p<0.05). 4) The size of ventricle increased in th patients who had IVH and the EVD was more frequently performed in th patients who had the enlarged ventricle associated with IVH (p<0.05). 5) In the patients who had high Fisher's grade, the size of ventricle had a tendency to increase, but there was no statistical significance. 6) In the patients who had poor prognosis, the size of ventricle had a tendency to increase, but there was no statistical significance. Conclusively, the initial size of ventricle was enlarged in th aged patients, ruptured aneurysm on anterior communicating artery, high FIsher's grade, patients associated with IVH. Considering those factors, prediction for the enlargement of ventricle size may help to treat the patients suffering aneurysmal SAH.

Predictive Factors of Death or Transplantation in Patients with Severe Alcoholic Hepatitis Treated with Prednisolone

( Young Seok Doh ),( Seong Kyun Na ),( Eui Ju Park ),( Kang Mo Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Corticosteroids have been shown to significantly decrease short-term mortality in severe alcoholic hepatitis with Maddrey’s discriminant function (MDF) score ≥32. However, independent clinical factors associated with unfavorable outcome during steroid therapy are not known definitely. The aim of this study was to investigate predictive factors associated with death or liver transplantation in patients with severe alcoholic hepatitis treated with prednisolone. Methods: A total of 134 consecutive patients treated with prednisolone for severe alcoholic hepatitis (MDF score ≥32) in Asan Medical Center between May 2004 and May 2014 were evaluated retrospectively. Survivals at 28 days were compared by Kaplan-Meier curve and log-rank test. The risk of death or transplantation was assessed by the Cox proportional hazards regression model. Results: The median age of the patients was 48 years (range, 24-73), and 105 (78.4%) patients were male. During the 28 days period, 11 patients (8.2%) died, 10 (7.5%) received a liver transplant. The median follow-up duration was 5.3 months. Sex, hepatic encephalopathy, Model for End-stage Liver Disease (MELD) score, MDF at 7days, and early change bilirubin levels (ECBL) at 7 days were predictive factors by univatiate analysis. In multivariate analysis, female (HR, 3.023; 95% CI, 1244-7.347, p=0.015), MELD score >25 (HR, 3.238; 95% CI, 1.169-8.972, p=0.024), and absence of ECBL at 7dyas (HR, 6.579; 95% CI, 1.513-28.571, p=0.012) were highly associated with the risk of death or transplantation. Conclusions: Our study revealed that female, MELD >25 and absence of ECBL were poor predictive factors in patients with severe alcoholic hepatitis although they were treated with prednisolone. Especially, these patients need to be assessed and prepared for the liver transplantation.

Poster Session : PS 1426 ; Hemato-Oncology(Oncology) : Primary Splenic Angiosarcoma Involving Bone and Bone Marrow: A Case Report

( Young Seok Doh ),( Jin Won Mo ),( Kyung Jin Lee ),( Hye Kyung Lee ),( Jung A Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Primary splenic angiosarcoma is a rare, aggressive malignant neoplasm arising from splenic vascular endothelium showing a high metastatic rate and a poor prognosis. Case: A 54-year-old woman presented with back pain, anemia, and thrombocytopenia. Spine MRI revealed multiple osteolytic bone metastasis. Lobulated splenic enlargement with heterogeneous enhancement and amorphous calcification were identified on abdomen CT scan. Laboratory results revealed normocytic hypochromic anemia (7.9 g/ dl), thrombocytopenia (91 × 10^3/μl). Bone marrow biopsy suggested the involvement of solid tumor. On subsequent bone biopsy, it raise the suspect of angiosarcoma that presented clearly immunohistochemical vascular to myofibroblastic differentiation with strong immunoreactivities for factor VIII related antigen, CD31 & CD34. Through a laparoscopic splenectomy, primary splenic angiosarcomas were diagnosed in spleen and accessory spleen. The follow-up CT scan showed progressed bone metastasis and new metastatic lesions of liver and kidney. She has been treated with 1st line adriamycin and ifosfamide two times, and with 2nd line genexol and cisplatin, 8 times. She showed partial regression on CT scan after 2nd line chemotherapy. Discussion: In this case, it is noted that we found the pathologic clue of primary splenic angiosarcoma through bone marrow and bone biopsy before explorative surgery. It means that less invasive procedures showed a diagnostic efficacy. And there are no guidelines concerning adjuvant or palliative treatment and no definitely benefi-cial protocol of chemotherapy or radiotherapy. Splenectomy prior to rupture is an only therapy showing the long-term survival benefit. Therefore it is so interesting that she improved clinically and showed partial regression with chemotherapy. Conclusions: Therefore we report a rare case that histopathologically diagnosed primary splenic angiosarcomas in both spleen and accessory spleen with multiple metastasis including bone and bone marrow. It has been successfully controlled by chemotherapy for ten months.

Long-Term Clinical Outcome of Clostridium difficile Infection in Hospitalized Patients: A Single Center Study

( Young Seok Doh ),( You Sun Kim ),( Hye Jin Jung ),( Young Il Park ),( Jin Won Mo ),( Hyun Sung ),( Kyung Jin Lee ),( Young Ki Seo ),( Jeong Seop Moon ),( Seong Woo Hong ) 대한장연구학회 2014 Intestinal Research Vol.12 No.4

Background/Aims: Antibiotic usage and increasingly aging populations have led to increased incidence of Clostridium difficile infection (CDI) in worldwide. Recent studies in Korea have also reported increasing CDI incidence; however, there have been no reports on the long-term outcomes of CDI. We therefore investigated the long-term clinical outcomes of patients with CDI, including delayed recurrence, associated risk factors and mortality. Methods: Hospitalized patients diagnosed with CDI at Seoul Paik Hospital between January 2007 and December 2008 were included. Their medical records were retrospectively investigated. ‘Delayed recurrence’ was defined as a relapse 8 weeks after a successful initial treatment. Multivariate logistic regression analysis was performed to identify risk factors for the delayed recurrence. Kaplan-Meier curves were used to analyze mortality rates. Results: A total of 120 patients were enrolled; among them, 87 were followed-up for at least 1 year, with a mean follow-up period of 34.1±25.1 months. Delayed recurrence was observed in 17 patients (19.5%), and significant risk factors were age (over 70 years, P=0.049), nasogastric tube insertion (P=0.008), and proton pump inhibitor or H2-blocker treatments (P=0.028). The 12- and 24-month mortality rates were 24.6% and 32.5%, respectively. No deaths were directly attributed to CDI. Conclusions: Delayed recurrence of CDI was not rare, occurring in 19.5% of the study population. Although CDI-related mortality was not reported, 2-year (32.5%) mortality rate of CDI patients implies that a CDI diagnosis may predict severe morbidity and poor prognosis of the underlying disease. (Intest Res 2014;12:299-305)

Clinical Characteristics of Long Term Survivors Following Sorafenib Treatment for Unresectable Hepatocellular Carcinoma: Korean National Multi-Center Retrospective Cohort Study

( Young Youn Cho ),( Su Jong Yu ),( Hye Won Lee ),( Do Young Kim ),( Wonseok Kang ),( Yong-han Paik ),( Pil Soo Sung ),( Si Hyun Bae ),( Su Cheol Park ),( Young Seok Doh ),( Kang Mo Kim ),( Eun Sun Ja 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Sorafenib is the standard systemic therapy for treatment of advanced stage hepatocellular carcinoma (HCC), and progressive HCC after locoregional therapy. The efficacy and safety of the drug in clinical practice have been addressed by several real world studies. However, little data are available regarding clinical factors of good responders following sorafenib treatment. This study aimed to evaluate prognostic factors of long term survivors. Methods: This multi-center retrospective cohort study included 1,605 unresectable HCC patients who received sorafenib treatment between 2007 and 2014 in 9 tertiary centers in Korea. The patients were classified into long-term survivor group (survival longer than 2 years, n=246) or control group (n=1,359). The primary endpoint was prognostic factors on survival for overall patients. Secondary endpoints included time-to-progression and other safety profiles. Results: The patients were predominantly male (83.8%), chronic hepatitis B (77.3%), and Barcelona Clinic of Liver Cancer stage C (78.3%). The median overall survival was 9.0 months. After treatment, 12 patients (0.7%) achieved complete response, and 146 patients (9.1%) achieved partial response by mRECIST criteria. The prognostic factors predicting long-term survival were metformin use (hazard ratio [HR]=2.44; P=0.004), hand foot skin reaction (HR=1.77; P=0.001), and concomittent treatment with chemoembolization or radiotherapy (HR =2.59; P<0.001). Prognostic factors against long-term survival were child pugh score B (HR=0.375; P< 0.001), presence of extrahepatic metastasis (HR=0.625; P=0.002), main portal vein invasion (HR=0.625; P=0.002), and elevated AFP ( >1,000 ng/mL; HR=0.393; P<0.001). Conclusions: This large multi-center retrospective study showed that the objective response was 9.8%, and the proportion of long-term survivors was 15.3% in Korean patients. The prognostic factors predicting long-term survival after sorafenib treatment were hand foot skin reaction, metformin use, concomittent treatment, good liver function, absence of extrahepatic metastasis, low degree of portal vein thrombosis, and low AFP.

Procedural Bleeding Risks and Ways to Prevent Bleeding in Liver Cirrhosis

도영석 ( Young Seok Doh ) 대한간학회 2021 간학회 싱글토픽 심포지움 Vol.2021 No.2

Fine Mapping of the Rice Bph1 Gene, which Confers Resistance to the Brown Planthopper (Nilaparvata lugens Stal), and Development of STS Markers for Marker-assisted Selection

Cha, Young-Soon,Ji, Hyeonso,Yun, Doh-Won,Ahn, Byoung-Ohg,Lee, Myung Chul,Suh, Seok-Cheol,Lee, Chun Seok,Ahn, Eok Keun,Jeon, Yong-Hee,Jin, Il-Doo,Sohn, Jae-Keun,Koh, Hee-Jong,Eun, Moo-Young Korean Society for Molecular Biology 2008 Molecules and cells Vol.26 No.2

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

태반 추출물 주사 후 발생한 과민성 폐장염

이상석 ( Sang Seok Lee ),추영광 ( Young Kwang Choo ),방창석 ( Chang Seok Bang ),김윤섭 ( Youn Seup Kim ),박재석 ( Jae Seuk Park ),지영구 ( Young Koo Jee ),김도형 ( Doh Hyung Kim ),명나혜 ( Na Hye Myong ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.66 No.6

Human placenta contains various kinds of nutritional elements essential for embryonic development. Currently, human placenta extracts are widely overused in Korea to improve certain health conditions (postmenopausal syndrome, liver function, and cosmetic purposes) without scientific evidence that they actually work. The use of placenta extracts should be restricted, due to a lack of systematic research on the therapeutic effectiveness and adverse results from these treatments. While the common adverse effects that have been reported are fever, rash, itching, nausea, vomiting, breast pain, and rare cases of anaphylactic shock, there have been no reports of pulmonary complications such as hypersensitivity pneumonitis. Recently, we experienced a patient with hypersensitivity pneumonitis following a placenta extract injection. To our knowledge, this is the first case of hypersensitivity pneumonitis associated with placenta extract use.

선형유도전동기를 이용한 자기부상추진시스템의 슬립주파수 제어

이석영(Lee Seok-Young),박상욱(Park Sang Uk),목형수(Mok Hyung soo),임재원(Lim Jae-Won),박도영(Park Doh-Young) 전력전자학회 2015 전력전자학술대회 논문집 Vol.2015 No.07