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Monascus purpureus를 이용한 쌀로 부터 천연식용 색소생산에 관한 연구
변광의,장성근,고영주 순천향대학교 기초과학연구소 1995 순천향자연과학연구 논문집 Vol.1 No.1
Studies on the optimal conditions for the production of natural edible red pigments with Monascus purpureus Went were performed. The optimal pH of strain on agar medium was 5.5 and the optimum autoclaving time for rice medium was twenty minutes. Analysis of food components of the raw or the fermented rice showed a remarkable changes: the contents of components were increased in moisture, crude protein and crude fat after the fermentation, but decreased in starch. starch. There was no difference in ash content but the reducing sugar in the fermented rice was detectable. The color intensity of the fermented rice revealed a wide range of differences among the regions produced. The water activity of rice medium was decreased but colony counts were increased during the fermentation periods. In the ergosterol assay, the peaks of ergosterol standard and fermented rice revealed a similar retention time. Changes in food components during the fermentation periods were daily investigated: the contents in moisture, crude protein and reducing sugar were increased but in starch content decreased. The water soluble extracellular pigment and the alcohol soluble intracellular pigment were increased continuously but the water soluble total pigment decreased at 10 day. In effect of inorganic salts, (NH₄)₂SO₄. FeSO₄·7H₂O. NH₄NO₃. MnO₂. NaN₃ and KNO₃have stimulated the pigment production. The absorption spectra of pigment solutions exhibited the maximum at 400nm and 500nm On thin layer chromatogram, the red pigment preparation revealed five fractions {R₁(??) -R₄} and yellow pigment preparation consisted of single fraction(Y₁). These basic results will be applied for studies of the polyketide biosynthesis pathway, the innovation of solid-state bioreactor and the production of natural food ingredients.
변광의,장영상 순천향대학교 1991 논문집 Vol.14 No.1
고추올레오레진의 제조시 에탄올농도와 추출 온도 및 추출회수에 따른 수율, capsaicine함량, 색도의 변화를 조사한 결과는 다음과 같다. 즉, (1) 에탄올농도가 낮을수록 올레오레진의 수율은 증가하는 경향을 보였으며 capsaicne함량과 색도의 경우는 에탄올농도가 높을수록 추출율이 증가하였다. (2) 95% 에탄올을 추출용매로 하여 올레오레진을 제조시 수율은 추출온도가 높아질수록 증가하는 경향을 보였으나 capsaicine의 경우 추출온도가 증가함에 따라 추출율이 감소하였으며 색도는 추출율이 계속 증가하는 경향을 보였다. (3) 추출회수의 영향을 보면 수율은 1,2차 추출시 추출온도가 높을수록 증가하는 경향을 보였으며 3차 추출시는 거의 일정하였다. capsaicine함량은 추출회수와 추출온도가 증가할수록 감소하는 경향을 보였고 색도는 추출회수가 증가할수록 높아지는 경향을 보였으나 80℃이상의 고온에서는 오히려 감소하는 결과를 나타내었다. This experiment was carried out to study on the effects of ethanol concentration, extraction temperature and times on extration in the preparation of red pepper oleoresin(RPO). The yield, capsaicine content and color unit were analyzed in RPO. The yield of RPO was good at low ethanol concentration, but capsaicine content and color unit were increased when the ethanol concentration is high. When 95% ethanol was used to be a solvent to get RPO, the yield increased as the extraction temperature went up. But in teh case of capaicine, the extraction ratio decreased and the color unit increased as the extraction temperature went up. According to the extraction frequency, the yield of 1st and 2nd extraction increased as the extraction temperature went up but that of 3rd extraction was constant. The content of capsaicine decreased as the extraction temperature went up. And the color unit increased as the extraction frequency went up but it decreased above 80℃ adversely.
( Sang Soo Lee ),( Young Sang Byoun ),( Mun Hyuk Seong ),( Hyung Rae Sohn ),( Bo Young Min ),( Eun Sun Jang ),( Jin Wook Kim ),( Sook Hyang Jeong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: The objective of this study was to investigate the characteristics and outcomes of hepatocellular carcinoma (HCC) in patient with nonalcoholic fatty liver disease (NAFLD) in Korea, which were compared to HCC related to hepatitis C virus (HCV) or to alcohol drinking. Methods: A total 424 HCC patients were consecutively enrolled in the hospital from January 2003 to December 2011. NAFLD was attributable to 4.7% of HCC (20 patients), while HBV to 68.2% (n=289)), HCV to 11.6% (n=49), and alcohol to 15.6% (n=66). Retrospective analysis of dermographics, liver function, tumor characteristics, treatment of initial choice and survival rate in NAFLD-HCC group was performed in comparison with HCV-HCC or Alcohol-HCC group. Results: NAFLD-HCC patients showed mean age of 69.9 year, proportion of male (55.0%), obesity (25.0%), diabetes (40. 0%), hypertension (55.0%), and hypercholesterolemia (10.0%) at diagnosis. Moreover, NAFLD-HCC group presented significantly higher rate of single nodular tumor (75.0% vs. 47.0%, p=0.032) and lower rate of portal vein thrombosis (5.0% vs. 28.8%, p=0.027) than HCV-HCC group, respectively. The single nodular presentation was significantly higher in NAFLD-HCC than alcohol-HCC group (75.0% vs. 38.8%, p=0.028, respectively), too. However, there were no significant differences in Child Pugh class, tumor stages (TNM, JIS, CLIP, and BCLC) and overall cumulative survival rate either between NAFLD-HCC and HCV-HCC, or NAFLD-HCC and alcohol-HCC group. Conclusions: NAFLD may take about 5% of current HCC etiology in Korea. The clinical characteristics of NAFLD-HCC were frequent company by metabolic features, single nodular tumor presentation with less frequent portal vein invasion. Further study is warranted to elucidate the biological characteristics of NAFLD-HCC.
( Sang Soo Lee ),( Young Sang Byoun ),( Mun Hyuk Seong ),( Hyung Rae Sohn ),( Bo Young Min ),( Eun Sun Jang ),( Jin Wook Kim ),( Sook Hyang Jeong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: The rs738497 single nucleotide polymorphism (SNP) of PNPLA3 is associated with hepatic steatosis and severity of liver disease. We aimed to study the genotypic frequency of rs738497 SNP in a Korean population and to investigate the association between this SNP and severity of nonalcoholic fatty liver disease (NAFLD) in Korea. Methods: The PNPLA3 SNP rs738497 genotype was analyzed using a TagMan 5’ allelic discrimination assay in a total of 334 adults (197 with NAFLD patients and 137 health-check examinees). NAFLD patients were classified as NAFLD without or with cirrhosis. Results: The CC genotype was found in 45 patients (32.8%) and CG+GG type in 92 patients (67.2%) in the health check-examinee group. The CC genotype was found in 50 patients (25.4%) and CG+GG type in 147 NAFLD patients (74.6%), which was not different from health check-examinee group. However, cirrhotic NAFLD patients (n=18) showed significantly higher CG+GG genotype frequency (94.4%, n=17) than that (72.6%, n=130) in noncirrhotic NAFLD by dominant model of inheritance (p=0.047). Multivariate analysis results showed that male, hypertension, and CG+GG dominant allele were the independent factors associated with development of cirrhotic NAFLD. Conclusions: About two-thirds of Korean population showed the CG+GG genotype in PNPLA3 SNP rs738497, and the CG+GG genotype may confers higher risk of liver cirrhosis in NAFLD.
Original Articles : Type and cause of Liver disease in Korea: single-center experience, 2005-2010
( Sang Soo Lee ),( Young Sang Byoun ),( Sook Hyang Jeong ),( Yeo Myung Kim ),( Ho Gil ),( Bo Young Min ),( Mun Hyuk Seong ),( Eun Sun Jang ),( Jin Wook Kim ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.3
Background/Aims: The aim of this study was to describe the types and causes of liver disease in patients from a single community hospital in Korea between April 2005 and May 2010. Methods: A cohort of patients who visited the liver clinic of the hospital during the aforementioned time period were consecutively enrolled ( n=6,307). Consistent diagnostic criteria for each liver disease were set by a single, experienced hepatologist, and the diagnosis of all of the enrolled patients was confirmed by retrospective review of their medical records. Results: Among the 6,307 patients, 528 (8.4%) were classified as acute hepatitis, 3,957 (62.7%) as chronic hepatitis, 767 (12.2%) as liver cirrhosis, 509 (8.1%) as primary liver cancer, and 546 (8.7%) as a benign liver mass or other diseases. The etiologies in the acute hepatitis group in decreasing order of prevalence were hepatitis A (44.3%), toxic hepatitis (32.4%), other hepatitis viruses (13.8%), and cryptogenic hepatitis (9.1%). In the chronic hepatitis group, 51.2% of cases were attributed to viral hepatitis, 33.3% to nonalcoholic fatty liver disease, and 13.0% to alcoholic liver disease (ALD). Of the cirrhoses, 73.4% were attributable to viral causes and 18.1% to alcohol. Of the hepatocellular carcinoma cases, 86.6% were attributed to viral hepatitis and 11.6% to ALD. Among the benign tumors, hemangioma comprised 52.2% and cystic liver disease comprised 33.7%. Conclusions: Knowledge of the current status of the type and cause of liver disease in Korea may be valuable as a basis for evaluating changing trends in liver disease in that country. (Clin Mol Hepatol 2012;18:309-315)
( Sang Soo Lee ),( Young Sang Byoun ),( Mun Hyuk Seong ),( Hyung Rae Sohn ),( Bo Young Min ),( Eun Sun Jang ),( Jin Wook Kim ),( Sook Hyang Jeong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: The objective of this study was to investigate the characteristics and outcomes of hepatocellular carcinoma (HCC) in patient with nonalcoholic fatty liver disease (NAFLD) in Korea, which were compared to HCC related to hepatitis C virus (HCV) or to alcohol drinking. Methods: A total 424 HCC patients were consecutively enrolled in the hospital from January 2003 to December 2011. NAFLD was attributable to 4.7% of HCC (20 patients), while HBV to 68.2% (n=289)), HCV to 11.6% (n=49), and alcohol to 15.6% (n=66). Retrospective analysis of dermographics, liver function, tumor characteristics, treatment of initial choice and survival rate in NAFLD-HCC group was performed in comparison with HCV-HCC or Alcohol-HCC group. Results: NAFLD-HCC patients showed mean age of 69.9 year, proportion of male (55.0%), obesity (25.0%), diabetes (40.0%), hypertension (55.0%), and hypercholesterolemia (10.0%) at diagnosis. Moreover, NAFLD-HCC group presented significantly higher rate of single nodular tumor (75.0% vs. 47.0%, p=0.032) and lower rate of portal vein thrombosis (5.0% vs. 28.8%, p=0.027) than HCV-HCC group, respectively. The single nodular presentation was significantly higher in NAFLD-HCC than alcohol-HCC group (75.0% vs. 38.8%, p=0.028, respectively), too. However, there were no significant differences in Child Pugh class, tumor stages (TNM, JIS, CLIP, and BCLC) and overall cumulative survival rate either between NAFLD-HCC and HCV-HCC, or NAFLD-HCC and alcohol-HCC group. Conclusions: NAFLD may take about 5% of current HCC etiology in Korea. The clinical characteristics of NAFLD-HCC were frequent company by metabolic features, single nodular tumor presentation with less frequent portal vein invasion. Further study is warranted to elucidate the biological characteristics of NAFLD-HCC.
( Sang Soo Lee ),( Young Sang Byoun ),( Mun Hyuk Seong ),( Hyung Rae Sohn ),( Bo Young Min ),( Eun Sun Jang ),( Jin Wook Kim ),( Sook Hyang Jeong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: The rs738497 single nucleotide polymorphism (SNP) of PNPLA3 is associated with hepatic steatosis and severity of liver disease. We aimed to study the genotypic frequency of rs738497 SNP in a Korean population and to investigate the association between this SNP and severity of nonalcoholic fatty liver disease (NAFLD) in Korea. Methods: The PNPLA3 SNP rs738497 genotype was analyzed using a TagMan 5` allelic discrimination assay in a total of 334 adults (197 with NAFLD patients and 137 health-check examinees). NAFLD patients were classified as NAFLD without or with cirrhosis. Results: The CC genotype was found in 45 patients (32.8%) and CG+GG type in 92 patients (67.2%) in the health check-examinee group. The CC genotype was found in 50 patients (25.4%) and CG+GG type in 147 NAFLD patients (74.6%), which was not different from health check-examinee group. However, cirrhotic NAFLD patients (n=18) showed significantly higher CG+GG genotype frequency (94.4%, n=17) than that (72.6%, n=130) in noncirrhotic NAFLD by dominant model of inheritance (p=0.047). Multivariate analysis results showed that male, hypertension, and CG+GG dominant allele were the independent factors associated with development of cirrhotic NAFLD. Conclusions: About two-thirds of Korean population showed the CG+GG genotype in PNPLA3 SNP rs738497, and the CG+GG genotype may confers higher risk of liver cirrhosis in NAFLD.
조소영 ( So Young Jo ),김나영 ( Nayoung Kim ),이정원 ( Jung Won Lee ),임지환 ( Ji Hwan Lim ),최치언 ( Chiun Choi ),전일영 ( Il Young Chon ),길호 ( Ho Kil ),민보영 ( Bo Young Min ),변영상 ( Young Sang Byoun ),이반석 ( Ban Seok Lee 대한소화기학회 2012 대한소화기학회지 Vol.59 No.6
Background/Aims: The aim of this study was to compare polyethylene glycol (PEG) 4 L, split method of PEG 4 L and PEG 2 L plus sodium phosphate (NaP) in the aspect of bowel preparation quality, safety, patients` compliance and preference. Methods: Total 249 subjects were prospectively enrolled and received bowel preparation for colonoscopy from August to October in 2010; PEG 4 L (93 subjects), split method of 4 L PEG (74 subjects) and PEG 2 L plus NaP 90 mL group (82 subjects). To investigate the completion, preference for bowel preparation and safety, a questionnaire survey was conducted before colonoscopy. Results: There were no significant intergroup differences in the aspect of completion of preparation, cecal intubation time and success rate. Satisfaction and preference were higher in PEG 2 L plus NaP 90 mL and split method of 4 L PEG compared with PEG 4 L. In the aspect of the bowel preparation quality PEG 4 L showed significantly higher quality in the morning colonoscopy (p<0.001). However, in the afternoon colonoscopy PEG 2 L plus NaP 90 mL showed better result than PEG 4 L (p=0.009). Hyperphosphatemia was most frequently observed in PEG 2 L plus NaP 90 mL, but no severe adverse events occurred (p<0.001). Conclusions: PEG 4 L showed better result than split method of 4 L PEG or PEG 2 L plus NaP 90 mL in the aspect of bowel preparation quality and safety. (Korean J Gastroenterol 2012;59:414-422)