국내 상급종합병원과 종합병원 간호사의 정맥주입간호실무지침의 확산정도

은영(Young Eun),구미옥(Mee Ok Gu),조용애(Young Ae Cho),정재심(Jae Shim Jeong),권정순(Jeong Soon Kwon),유정숙(Cheong Suk Yoo5),정영선(Young Sun Jeong),정인숙(In Sook Jung),김경숙(Kyeong Sug Kim),이선희(Seon Heui Lee),서현주(Hyun Ju S 한국근거기반간호학회 2015 근거와 간호 Vol.3 No.1

Purpose: This study was conducted to investigate the extent of diffusion of Intravenous infusion nursing practice guideline among nurses in advanced general hospitals and general hospitals in Korea. Methods: The subjects were 234 nurses who practice the intravenous infusion in 24 advanced general hospitals and general hospitals. Data were collected between October 5 and November 2, 2015 by mail (return rates: 97.5 %). Data were analyzed using descriptive statistics, t-test, and ANOVA with SPSS/ WIN 21. Results: The average guideline diffusion score and levels of diffusion was 3.39±0.58 (level of “use sometimes”). 44 recommendations (46%) were in level of “use always” and 50 recommendations (53%) were in “use sometimes”. Extent of diffusion were significantly different according to present status (F=2.81, p=.040) and education (F=4.35, p=.014). The facilitating factors to use the guideline were education by department of nursing service, convenient composition of guideline and barrier factors were “no time to use the guideline”, “don’t know the guideline” and “there is no guideline at ward”. Conclusion: Extent of diffusion of Intravenous infusion nursing practice guideline among nurses was moderate level. The strategies for promoting the implementation of guideline in clinical practice is needed. Further study of outcome evaluation of guideline implementation will be needed.

혈청 감마지티(gamma glutamyl transferase)치와 대사증후군 연관성

장미,유병욱,조용진,오정은,홍성호,조주연 순천향대학교 의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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혈청 감마지티(gamma glutamyl transferase)치와 대사증후군의 연관성

장미,유병욱,조용진,오정은,홍성호,조주연 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

Background: Serum gamma glutamyl transferase(GGT) level has been widely used as an index of liver dysfunction and a marker of alcohol intake. But, recently it is identified that the elevated GGT level was associated with insulin resistance. This study aimed to examine the association between metabolic syndrome and elevated GGT level in the women who had less alcoholic influence generally. Methods: From 585 women who received their annual health check-up, 577 women were selected for analysis except for 28 women who had hepatitis B surface antigen positive or antibody to hepatitis C virus positive, Categories of serum GGT were classified into four groups by quartile and then evaluated. Results: A abnormal GGT level(≥52U/L) was seen in 29(5.2%) of the 557 women. As GGT level increased, the prevalence rate of factors(body mass index≥25kg/m2, hypertension, hemoglobin≥14g/dL, total cholesterol≥220mg/dL, triglyceride≥150mg/dL, fasting plasma glucose≥110mg/dL, HDL-choles tero1<50mg/dL) except for hyperuhcemia was increased. The GGT level was correlated with age, body mass index, thglyceride and uric acid in women with fatty liver, but not in women without fatty liver. Factors independently contributing to elevated GGT level was diabetes, hyperthglycehdemia and obesity. Conclusion: The elevated, albeit normal, GGT level was strongly associated with the factors of metabolic syndrome in Korean women. Because measurement of serum GGT level is easy, reliable, and not expensive, it might have more important implication as predictive factor of metabolic syndrome.

종합병원 간호사의 밤번 근무에 영향을 미치는 요인에 관한 연구

박혜자,유인영,이영미,정문희 한국보건통계학회 2003 보건정보통계학회지 Vol.28 No.1

This study is conducted to find influencing factors on unrse's night shift works. From 20th July to 20th August, the survey was performed in a general hospital located at Kyunggi-do area, by the use of questionnaire. Collected 204 data were analysed through SPSS/Win 11.0. Results are as follows: 1. It was shown that night shifting word periods in special ward is longer than that in general ward. Exactly, shifting periods of night nurse work is 28.7days, 16.7days in special and general ward, individually. But numbers of night nurse worker are 3.4 and 2.48 persons in special and general ward, individually. And there were more patients in general wards than that in special ward. Namely, 54.3 patients in general ward and 19.3 patients in special ward. And off duty days and overtime on average in general ward were more much than that in special ward. The preference about night nurse works, the general ward was higher than that in general wards. In case of avoiding the night nurse work, the reasons that were mental stress. excess of duty, the burdens on work by oneself in general wards were shown much more frequency than that in special ward. 2. Physical-mental, social conditon of nurse worker's health in special wards were better than that in general wards. The stress level of nurse workers in general ward was higher than that in special ward. The factors of the influence on the preference on social-mental conditions of health were number of off duty days on average per month, number of night nurse working, and income of all family. Therefore, theses results were shown that the stress of nurses in general ward was much more than that in special ward. And the preference on night nurse work in general ward was lower than that in special ward. Accordingly, It was shown that the policy for support were need. The nurse administrator have to prepare complementary systems in organized level, to develop the positive recognition about night nurse work. For example have to reduce the dissatisfaction with systematic improvement, in other words control of off duty bonus, reasonable overtime and night nurse work bonus, and night working periods, and with control nursing human power and policy for reduction of overstres according working conditions and characters of business in various types of ward.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

POSTERS / PW-096 : THE NEURAL RESTRICTIVE SILENCER ELEMENT REGULATES DYNAMIN 1 GENE PROMOTER ACTIVITY FOR NEURON - SPECIFIC EXPRESSION

(Young Mee Park),(Moon Jin Jeong),(Mi Young Han),(Ji Yun Yoo),(Sang Seop Lee),(Kyung Im Lee),(Dong Sun Kim) 한국생화학분자생물학회 (구 한국생화학회) 1999 6th IUBMB SEOUL CONFERENCE Vol.- No.-

Alterations of Tyrosine Hydroxylase and Choline Acetyltransferase in the Retina of the Diabetic Rat

최미영(Mee-Young Choi),김영희(Young-Hee Kim),김윤숙(Yoon-Sook Kim),남희(Hee Nam),유지명(Ji-Myung Yoo),최완성(Wan-Sung Choi),조경제(Gyeong-Jae Cho) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.6

To investigate the effect of hyperglycemia on the visual system, we investigated the retinal dopaminergic and cholinergic systems using tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) in the rat retinas of streptozotocin (STZ)-induced diabetes. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg) to Sprague-Dawley rats (250~300 g). We first analyzed morphologic thickness changes in the several retinal layers of 6-week-old control and STZ-diabetic rats after H & E staining. To confirm whether TH and ChAT protein expressions changed, we carried out immunohistochemistry analysis and Western blotting. After induction of diabetes, significant changes were not shown in the retinal thickness at 6 weeks. TH and ChAT immunoreactivities were clearly detected in amacrine cells and sublaminas in the inner retina of both control and diabetic rats, showing continuously reduced positive amacrine cells in the retinas during diabetes. In addition, the decline in TH and ChAT protein expression was already present to a significant extent in the retina at 6 weeks in early diabetes. Our present study demonstrates the possibility that the observed alterations in TH and ChAT in the diabetic retina may cause the visual system changes in the retinal pathophysiology associated with diabetes mellitus. 본 연구에서는 고혈당이 시각의 dopaminergic 및 cholinergic system에 미치는 영향을 알아보기 위하여 streptozotocin (STZ)으로 유도한 당뇨병 모델 흰쥐의 망막에서 tyrosine hydroxylase (TH)와 choline acetyltransferase (ChAT)의 변화를 알아보았다. 당뇨병은 250~300 g Sprague-Dawley (SD) 흰쥐에 50 mg/kg STZ을 복 막내 주사하여 유도하였고 6주 후에 희생시켰다. H & E 염색을 통하여 정상쥐와 당뇨쥐 망막의 형태학적인 변화를 관찰하였고, 면역조직화학법과 western blotting을 이용하여 TH와 ChAT 단백질의 발현과 변화를 분석하였다. 당뇨병 유발 6주 후 흰쥐의 망막에서 뚜렷한 형태학적인 변화는 나타나지 않았으나 TH와 ChAT의 단백질 발현과 면역반응성의 유의적인 감소가 확인되었다. 본 연구는 TH와 ChAT의 변화가 초기 당뇨 흰쥐 망막의 시각시스템변화와 연관되어 있음을 시사한다.

Loss-of-function screens of druggable targetome against cancer stem–like cells

Song, Mee,Lee, Hani,Nam, Myung-Hee,Jeong, Euna,Kim, Somin,Hong, Yourae,Kim, Nayoung,Yim, Hwa Young,Yoo, Young-Ji,Kim, Jung Seok,Kim, Jin-Seok,Cho, Yong-Yeon,Mills, Gordon B.,Kim, Woo-Young,Yoon, Sukjo Federation of American Societies for Experimental 2017 The FASEB Journal Vol.31 No.2

<P>Cancer stem–like cells (CSLCs) contribute to the initiation and recurrence of tumors and to their resistance to conventional therapies. In this study, small interfering RNA (siRNA)-based screening of ∼4800 druggable genes in 3-dimensional CSLC cultures in comparison to 2-dimensional bulk cultures of U87 glioma cells revealed 3 groups of genes essential for the following: survival of the CSLC population only, bulk-cultured population only, or both populations. While diverse biologic processes were associated with siRNAs reducing the bulk-cultured population, CSLC-eliminating siRNAs were enriched in a few functional categories, such as lipid metabolism, protein metabolism, and gene expression. Interestingly, siRNAs that selectively reduced CSLC only were found to target genes for cholesterol and unsaturated fatty acid synthesis. The lipidomic profile of CSLCs revealed increased levels of monounsaturated lipids. Pharmacologic blockage of these target pathways reduced CSLCs, and this effect was eliminated by addition of downstream metabolite products. The present CSLC-sensitive target categories provide a useful resource that can be exploited for the selective elimination of CSLCs.—Song, M., Lee, H., Nam, M.-H., Jeong, E., Kim, S., Hong, Y., Kim, N., Yim, H. Y., Yoo, Y.-J., Kim, J. S., Kim, J.-S., Cho, Y.-Y., Mills, G. B., Kim, W.-Y., Yoon, S. Loss-of-function screens of druggable targetome against cancer stem–like cells.</P>

원어민 교사 회화 수업에 나타난 대학생들의 말하기 불안 연구

유영미 ( Young Mee Yoo ),표경현 ( Kyong Hyon Pyo ) 한국외국어대학교 외국어교육연구소 2009 외국어교육연구 Vol.23 No.2

본 연구는 대학생들이 원어민 교수 수업에 참여하는 동안 어떠한 말하기 불안감을 느끼고 있고 그 주된 불안의 원인은 무엇인지 살펴보고자 하였다. 본 연구자는 449명의 원어민 회화 수업 수강생들을 대상으로 내부귀인과 외부귀인에 따른 불안을 범주화하여 학습자들의 불안 연구를 통해 그 양상을 살펴보았다. 본 연구를 통하여 분석한 결과는 다음과 같다. 첫째, 원어민 회화 수업에서 대부분의 학생들이 불안의 원인을 자신의 의사소통 능력 부족으로 귀인하였다. 둘째, 외부귀인에 있어서 교수나 동료 학생의 부정적인 평가로부터 오는 불안의 정도는 크지 않았으나 교사의 긍정적인 평가에 대한 학습자들의 동기부여에는 매우 크게 영향을 미치는 것으로 나타났다. 셋째, 학습자들은 오류 없는 완벽한 말하기를 해야 한다고 생각하지 않으나, 예고 없이 말하기를 해야 하는 상황에서 매우 높은 불안을 보였다. 넷째, 설문지 결과 학생들은 개개인이 지목당하여 말하는 때보다 다 같이 교수자가 말한 것을 그대로 따라 하는 경우에 말하기 불안 수준이 낮은 것으로 나타났다. 학습자들의 말하기 불안이 가장 낮은 상태에서 회화 수업을 진행할 수 있다면 학습자들의 학업 성취도도 높일 수 있으므로 연구를 통하여 나타난 위의 결과를 참고하여 원어민 교수에게 적절한 자료를 제시함으로써 원어민 교수들에게는 학습자들의 불안에 대한 이해의 기초와 학습자들의 말하기 불안 요인을 낮출 수 있는 수업방안을 제공하고 학습자들에게는 적절한 학습방식을 제공하여 원어민 교사의 수업운용이 갖는 효용성을 적극 살릴 수 있도록 이에 대한 더 많은 연구와 개선방안의 제시가 필요함을 시사하고 있다. The main purpose of this study is to understand the sources of college students` speaking anxiety in English conversation classes with native English speaker instructors. The researchers asked 449 students who have previously taken conversation classes with a native English speaker instructor to complete questionnaires and participate in interviews. Speaking anxiety in these classes was found to be the result of a lack of linguistic or communicative competency on the part of the students. It was also found that negative feedback from fellow students or the teacher was not an essential factor causing students` high speaking anxiety. However, positive feedback from the teacher was shown to be important in increasing student confidence and building motivation to study English. It was also found that even though students were free to make errors during the classes, they nevertheless felt embarrassed when called on to speak English abruptly. The students reported that they were indifferent about actually being able to speak English without errors, and the students preferred group or pair work within the class so as to lower their anxiety. This result implies that native speaker English teachers might consider using group or pair work in order to reduce student anxiety.

Bacteroides fragilis Enterotoxin Induces Human β-Defensin-2 Expression in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase/IκB Kinase/NF-κB-Dependent Pathway

Yoon, Young Mee,Lee, Jin Young,Yoo, Doyoung,Sim, Young-Suk,Kim, Young-Jeon,Oh, Yu-Kyoung,Kang, Ju Seop,Kim, Sunil,Kim, Joo Sung,Kim, Jung Mogg American Society for Microbiology 2010 Infection and immunity Vol.78 No.5

<B>ABSTRACT</B><P>Enterotoxigenic <I>Bacteroides fragilis</I> (ETBF) produces an approximately 20-kDa heat-labile enterotoxin (BFT) that plays an essential role in mucosal inflammation. Although spontaneous disappearance of ETBF infection is common, little information is available on regulated expression of antibacterial factors in response to BFT stimulation. This study investigates the role of BFT in human β-defensin 2 (hBD-2) induction from intestinal epithelial cells. Stimulation of HT-29 and Caco-2 intestinal epithelial cell lines with BFT resulted in the induction of hBD-2. Activation of a reporter gene for hBD-2 was dependent on the presence of NF-κB binding sites. In contrast, suppression of AP-1 did not affect hBD-2 expression in BFT-stimulated cells. Inhibition of p38 mitogen-activated protein kinase (MAPK) using SB203580 and small interfering RNA (siRNA) transfection resulted in a significant reduction in BFT-induced IκB kinase (IKK)/NF-κB activation and hBD-2 expression. Our results suggest that a pathway including p38 MAPK, IKK, and NF-κB activation is required for hBD-2 induction in intestinal epithelial cells exposed to BFT, and may be involved in the host defense following infection with ETBF.</P>