위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

CROSS 공법을 이용한 폐수의 생물학적 처리에 관한 연구

박광하,심재도,권영두,강동수,김인호,정태명,황갑성 7개 국립대학교 환경연구 논문집 공동발행 위원회 2003 공업기술연구 Vol.3 No.-

This study aims to develop using a continuous removal of organic and settling solid(CROSS) to increase the efficiency of aeration system for wastewater treatment. To solve many problems of the conventional activated-sludge process, authors suggested a new CROSS model in conjunction with aeration systems in 1996. This system is characterized by utilizing an intensive aeration pipe with inlets at the lower portion and outlets at the higher portion in an aeration tank. Through the experiments on CROSS process, the average removal efficiences of BOD, COD_(Mn), SS, T-N and T-P were showed 98.8%, 93.9%, 98.0%, 71.3% and 49.8%. The average DO (Dissolved-Oxygen) concentration was constantly maintained at 8.0 mg/L by the intensive aeration which was accompanied by rapid reduction of BOD. Degradation of the organic matters in sewage then continued for the whole aeration period. The process demonstrated that it was very adaptive without seeding and returning the sludge. Compared to the activated sludge system, the system was very flexible for the continuous flow treatment. This preliminary assessment showed that the porous media in CROSS reactor is viable for the sewage treatment, since the porous media under the intensive aeration achieves acceptable effluent quality.

두경부 악성종양 절제술 후 유리피판을 이용한 재건술 40예의 고찰

백정환,손영익,김성민,추광철,하범준,심영목 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

측두하악장애 환자에서 과두크기와 관절융기의 후방경사에 관한연구

박원희,이영수,우순섭,심광섭 大韓顎顔面成形再建外科學會 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.1

The purpose of this study was to investigate the interrelationship between temporomandibular joint disorders, and the condyle size and angle of the posterior slope of the articular eminence. The subjects used in this study were 100 patients with temporomandibular joint disorders and 100 volunteers with normal temporomandibular joints. All the patients and the volunteers were subjected to take panoramic and temporomandibular radiographs for the morphologic evaluation. This films were traced, measured, and analyzed. The data were processed with SPSS/PC+package for statistical analysis. The obtained results were as follows : 1. The posterior slope of the articular eminence in the group of temporomandibular joint disorders was larger than that in the normal group (p<0.05). The mean articular eminence angle was 31.6。±6.3 in the group of temporomandibular joint disorder, and 29.9。±8.4 in the normal group. 2. There were no statistically significant differences in the discrepancies of the left and right articular eminence angular measurements between the group (p>0.05). 3. There were no statistically significant differences in the condylar ratio between the groups (p>0.05). 4. There were no statistically significant differences in the discrepancies of the left and right ramus length measurements between the groups (p>0.05). 5. The relative size of condyle to fossa in the group of temporomandibular disorders was smaller than that in the normal group(p<0.05).

구강 및 악안면 영역의 감염증에 관한 임상적 연구

백진현,이영수,심광섭 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.2

This is a retrospective study on the patients with infection of the oral and maxillofacial region with the purpose of obtaining some useful data for diagnosis and treatment plan of that relatively common disease in dentistry. The used materials of study were 87 in total, including 52 male patients, 35 female patients who diagnosed and treated at the Department of the Dentistry in Hanyang Medical College Hospital for the period of Jan. 1990 to Dec. 1994. The author analyzed the distribution and incidence of sex, age, admission period, etiologic factors, etiologic teeth, treatment method of infections, pus culture, antibiotics sensibilities and medication. The result obtained as follows : 1. The developmental incidences by sex was superior in male by the ratio of 1.5 : 1 and the infection was most frequently occurred during the third decades(35.6%). 2. The number of admitted patients elevated in February, March, and April, and average of admission period was 9.8 days. 3. Main etiologic teeth showed on lower molar region in adult(63%) and upper molar region in primary dentition(46.1%). 4. Medications were administrated in all of the cases, and surgical incision and drainage were performed in 53% and extraction of the causative teeth were performed in 63.6% of all cases. 5. The most common involved fascial spaces were Buccal space(41.4%), Infraorbital space(27.6%), Submandibular space(16.1%),in order, and 9 cases(10.3%) were Ludwig's Angina. In 68.2% of the patients, and infection involved only one fascial space and in 21.8% of the patients, it involved to more fascial spaces. 6. The most causative organisms isolated from pus culture were Gram-positive facultative cocci(55.5%), and antibiotics sensitivities on the total isolated bacterial strains were exposed chloramphenicol(88.6%), Cephalothin(88.6%), Erythromycin(81.5%), Lincomycin(77.8%) in order, but it showed resistant on Gentamycin(58.3%), Tetracycline(56.5%), Methicillin(38.5%).

Chitosan 濾過 System을 利用한 새로운 中水道 工程의 開發과 溫泉水 淨化에 관한 硏究

김용범,백광욱,안병제,조영주,심재용 서울産業大學校 1994 논문집 Vol.39 No.1

각종 금속이나 중금속을 비롯하여 각종 오염물(SS, COD, BOD, CI 등)을 강력히 흡착제거하는 성능을 가진 chitosan(Cs)응집제와 여과사(SF) 및 활성탄 column(ACC)을 병용하여 hotel잡수와 온천사용수등의 정화실험을 행하여 생물학적 처리과정을 생략할 수 있는 사용수의 재활용 process(Fig. 1)를 개발하였다. 이 process에서 hotel 잡수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 60%이상의 오염물을 제거한 후 여과사와 활성탄 column을 이용하여 최종적으로는 80~99%의 오염물을 제거할 수 있었다. 또한 온천수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 50%이상의 COD와 BOD의 제거율을 달성할 수 있었다. 따라서 이러한 process를 산업현장에 적용할 경우 종전의 생물학적 공정을 생략하거나 소규모화하여 보다 간편하며 처리공간이나 경비등을 절감하면서도 사용수를 방류수 또는 재활용수 기준이내로 처리할 수 있음을 확인하였다. 본 연구는 수자원 보호나 수자원 재활용면에서 크게 기여 되리라 믿는다. A simplifid and economic process for recycling the used water discharged from a large scale residence(e. g. hotel and apartment etc.) was developed. The used water was purified through adsorption and flocculation of contaminants by chitosan at the first stage where the average removal rates of COD and BOD of the used water were found more than 60%. The previously treated water through flocculation by chitosan was subjected to filtration systems of a sand filter and an activated carbon column, and the average removal rates of COD and BOD were found more than 80%. The experimental results suggest that the biological process which is very popular in the treatment of the used water for the removal of COD and BOD can be omitted and expected to reduce a spacial and running expenditure. On the other hand, testing the used water discharged from hot springs with chitosan proves a possibility of development of a simple and economical process for the treatment of the used water to the criteria of recycled water quality without any addition of more complicated equipments and facilities.

분무 열분해에 의한 Ba1-xSrxFe12O19계 자성 미립자의 제조 및 특성 변화

김동현,김광만,김경남,최세영,심인보,이용근 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

Magnetic particles with alternating magnetic field are expected to be useful as thermoseeds in hyperthermic cancer treatment, since they can be targeted and confined to the cancer site. Hard magnetic ferrites such as barium ferrite and strontium ferrite are good candidates for thermoseeds of hyperthermia because of their great hysteresis loss. In order to verify the effect of Sr-substitution to Ba-site, synthesized Ba1-xSrxFe12O19 microspheres with various compositions through spray pyrolysis followed by sol synthesis using barium nitrate, strontium acetate and iron nitrate. Coercive force was increased with increasing substitution amount of Sr whereas magnetic saturation was almost constant. Spherical microspheres with average diameter of 11.7~17.0 ㎛ were produced by spray pyrolysis at 400~1000℃. The mean size was dependent on the pyrolysis temperature and flow rate of carrier gas. In order to obtain a single phase of SrFe12O19 crystal, the spray pyrolyzed spheres were undertaken subsequent heat-treatment above 1000℃. During the subsequent heat-treatment, however, spherical microspheres were agglomerated because of necking between particles. Further study has to be continued working on synthesis of pure SrFe12O19 crystal by means of improvement of pyrolysis temperature scheme and extend the heating period.

치근단 병소의 임상적 연구

김상우,우순섭,유임학,이영수,심광섭 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

The purpose of this study was to find the clinical features of periapical lesions. A total of 130 periapical lesions which were obtained from biopsy and diagnosed histopathologically as periapical cyst, periapical abscess, and periapical granuloma at the Department of Dentistry in Hanyang University Hospital were throughly analysed according to the distribution and incidence of age, sex, location, and so on. The following results were obtained : 1.Out of 130 periapical lesions, 88 cases(67.7%) were periapical cysts, 30 cases(23.1%) were periapical abscesses, and 12 cases(9.2%) were periapical granulomas. 2.The periapical lesions occurred most frequently in the third decade, and followed by the fourth, fifth, sixth, and second decade. The periapical cysts occurred most frequently in the third decade(26.1%), the periapical granulomas in the fourth decade(33.3% ) the periapical abscesses in the sixth decade(26.7%). 3.The periapical lesions were more frequent in men than in women with the ratio of men to women of 1.4 : 1. The radio of men to women of periapical cysts was 1.6 : 1, that of periapical granulomas was 0.5 : 1, and that of periapical abscesses was 1.3 : 1. 4.The periapical lesions were more frequent in maxilla than in mandible with the ratio of 1.2 : 1. The ratio of maxilla to mandible of periapical cysts was 1.5 : 1, that of periapical granulomas was 0.5 : 1, that of periapical abscesses was 1 : 1. 5.The most commonly involved location of the periapical lesions was maxillary anterior teeth(40.8%), and followed by mandibular molars, mandibular premolars, and mandibular anterior teeth and maxillary molars. The most frequent location of the periapical cysts was maxillary anterior teeth(48.9%), that of periapical granulomas was mandibular molars(50.0%), that of periapical abscesses was mandibular molars(40.0%).

대장통과시간 측정용 방사선 비투과성 표지자인 KolomarkTM의 국내 개발

김지은,이풍렬,김영호,성인경,심상군,김재준,고광철,최규완,임효근,서수원 대한소화관운동학회 1999 Journal of Neurogastroenterology and Motility (JNM Vol.5 No.2