Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

뿜칠시공에 의한 단면복구재의 시공성 및 공학적 특성에 관한 실험적 연구

조봉석,김영선,장재봉,김용로,김경덕,김무한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

These days, serious concerns of reinforced concrete have been raised recently over steel-bar corrosion, cracking, the deterioration of durability and structural stress resistance and so on, all due to the effects of exterior factors. Such being the case, in domestic, various repair materials and method systems to keep up with these reinforced concrete deteriorated due to salt damage, carbonation, chemical decay et. developed and applied. However, on-site quality control of various repair materials and method systems isn't achieved desirably because it is depend completely on a men of experience' opinions above all else regardless of various on - site environments. In this background, mock up test with due regard to real on-site environments was performed to secure fundamental data for establishment of desirable on-site quality control. Mock up test using repair mortar analyzed from angles of construction methods, mechanical spraying pressures, W 1M. Construction methods were designed manpower method and spraying method, spraying pressures were designed 32, 42, 52 psi, W/M were designed 14.4, 15.4, 16.4 %. And compressive strength, Chloride ion diffusion coefficient, bond strength, SEM of mock up test specimens were evaluated. In conclusion, we confirmed excellency of mechanical spraying pressures, fined extremely excellency of condition of spraying pressure 42 ps, W/M 14.4% within this study. therefore the results of this study will be useful to provide fundamental data for establishment of desirable on-site quality control.

장기간 체중부하 운동훈련이 흰쥐 노화 심근에 미치는 영향

박원학,조현국,이영실,정형재,이용덕 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.2

장기간 체중부하운동이 흰쥐의 심근에 미치는 효과를 규명하기 위하여 생후 3개월, 10개월 및 20개월된 흰쥐를 5개월간 운동 시킨 후 각각의 비운동군과 비교하여 심근의 조직학적 ·세포학적 변화를 관찰하고, 심근세포내 미세구조적 변화를 입체해석학적으로 비교 ·분석하였다. 운동 15개월군부터 노화색소의 양이 현저히 증가되었고 사립체의 변성, 공포화 현상 그리고 간질조직의 증식이 뚜렷하였으며, 25개월군에서는 윤반분리, 근원섬유의 소실 및 과수축대 출현빈도가 증가되었다. Glucose-6-phosphatase의 활성도는 15개월 운동군에서 감소하여 25개월 운동군에서는 활성도가 거의 나타나지 알았다. 입체해석학적 결과에서도 장기간 체중부하운동으로 인해 15개월, 25개월군에서 모두 사립체와 근원섬유의 체적밀도는 감소되었고, 간질조직의 체적밀도는 증가되었다. 본 연구의 결과로 볼 때 장기간 체중부하운동은 3개월군에 있어서 심장에 미치는 역효과가 없으나 10개월군과 20개월군에서는 심근 기능강화에 역효과를 초래 할 우려가 큰 것으로 판단된다. There is considerable interests in the effect of regular, vigorous exercise, in particular weight training as a possible measure in improving myocardial function. The present investigation aimed to examine possible effect of a long-term weight training program on the heart in aging rats. Male rats aged 3-, 10-, and 20-month-old were divided at random into a control (sedentary) and the exercise group. The training group was exercised for 5 days a week by 1 RM of ten times with weight training apparatus. This investigation examined the changes of the heart muscle relative to histological, ultrastructural, cytochemical, and stereological studies in rats. Quantity of lipofuscin pigments was clearly increased in the weight training group of 15-month-old rats, and mitochondrial degeneration, vacuolization, and interstitial proliferation were observed as well. In the weight training group of 25-month-old rats the frequency as separated intercalated discs, fraying myofibrils and hypercontraction band increased in number compared with the same 15-month-old group. From the experimental result of glucose-6-phosphatase activity, the enzyme activities decreased in the weight training group of 15-month-old rats, and more decreased in the same 25-month-old group. In stereological study, both 15- and 25-month-old training groups, mitochondrial and myofibrilar volume densities significantly decreased, whereas interstitial volume density significantly increased. From the experimental results obtained in the present study, it is suggested that long-term weight training exercise do not cause any significantly qualitative and quantitative ultrastructural change of the heart muscle in the young. On the contrary, long-term weight training exercise stress may actually induce degenerative changes in the heart muscle in the old age.

더덕의 효율적인 재분화 및 뿌리발달

심주선,조숙녀,손화,김무성,노영덕,김세영,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

더덕은 식용, 관상용, 생약재료로 쓰이는 방향성 식물이다. 본 연구는, 종자의 기내배양을 통하여 식물체 재분화와 뿌리발달을 실험했다. 종자의 발아는 GA_(3) 100 mg·L(-1)처리와 저온처리 시 매우 양호하였다. 더덕의 기내에서 shoot의 형성은 2 mg·L^(-1) BA, 2 mg·L(-1) NAA가 첨가된 MS 기본배지에서 가장 양호하였으며, 줄기의 절편체와 비교하여 잎 절편체에서 재분화가 잘되었다. 또한 뿌리의 발육은 계속적인 계대배양에 의해서 양호하였는데 shoot의 길이를 1 cm 정도 남긴 뿌리를 연속적으로 4회 계대배양에서 뿌리가 주근으로 발육하였으며 길이는 약 3 cm 및 직경은 0.5 cm의 정상적인 뿌리를 얻을 수 있었다. Codonopsis lanceolata is widely used as edibles, ornamental plant and crude drug material. This study was carried out to confirm the ratio of germination and efficient differentiation of shoot and root in vitro. The germination of Codonopsis lanceolata seed was excellent in the medium with GA_(3) 100 mg·L(-1) and chilling treatment. The shoot formation of Codonopsis lanceolata was good at the MS medium with 2 mg·L(-1) BA and 2 mg·L(-1) NAA. According to inoculum part, ratio of re-differentiation was higher from the leaf explant compared with the stem explant. For root development, successive subculture with 1 cm shoot of upper part from root was efficient. Roots of Codonopsis lanceolata cultured in vitro system was grown like normal root with length of 3 cm and thickness of 0.5 cm at the 4 successive subcultures.

154kV용 활선 안전 경보기 개발에 관한 연구

정민규,조현광,우종수,안창회,서영석,김응태,박창기,오세득 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

In this report we developed a high voltage alarm for the safety of workers who check and repair the 154kV power line. This high voltage alarm we developed is attached on workers' helmet and alarms automatically in order to let workers know when workers work over the safety distance near 154kV power line. Until now the exact safety distance hasn't been regulated and high voltage alarm we've been using was for the range of 6∼22.9kV power line. We fabricated this high voltage alarm on the basis of electric density and actual measurement of power line. The experimental result was that high voltage alarm at 66kV power line alarmed near 90cm, at 154kV power line alarmed near 160cm from the line. Accordingly, we developed the new high voltage alarm which is operated in the range of 66∼154kV power line.

벼 상온 저장 시기별 쌀 품질 변화

권석주,송영은,권영립,이덕렬,조승현,최동철 원광대학교 2005 論文集 Vol.35 No.-

Increasing concern about a rice quality, it is considered postharvest technology as well as cultivation management as an important factor. Therefore it is necessary to find optimal drying, storage method that did not affected a rice quality. In order to find the optimum storage period of rice, the rice quality was investigated during storage period. The temperature and humidity of storage bin were greatly changed but those of rice were a little do. The germination rate was slowly decreased as the storage period is long. The content of moisture was affected by a room temperature, it was increased at stored rice in rainy season(10-Jul~20-Jul). As storage period is long, the content of amylose, fatty acidity were a just Little changed but did not showed a constant tendency to it. The content of protein was increased and mechanic palatability value was decreased during storage period. The chromaticity of milled rice showed a range of lightness(L); 75.15~71.75, redness(a); 0.21~0.91, yellowness(b); 16.78~19.28 during storage period.

전로제강 슬래그(BOF-Slag)를 이용한 고온탈황에 관한 연구

오광중,정덕영,최성원,조상원,손병현 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.51 No.-

고온 석탄연료가스의 탈황에 BOF-Slag의 이용가능성을 실험해 보았다. BOR-Slag의 온도, 유량, 탈황제의 특성등이 H₂S 제거효율에 미치는 영향을 조사해 본 결과, 최적의 H₂S 제거효율은 700℃에서 이루어졌고 입자크기가 작을수록 황포집능은 향상되었으나 0.214-0.631mm에서는 큰 영향이 발견되지 않았다. 조업온도 800℃이상에서는 탈황제의 응집으로 내부물질전달저항이 증가하여 탈황제의 반응성이 저하되었다. Experiments have been made to test the practical feasibility of using BOF-Slag to desulfurize hot coal-derived fuel gas. In this study, the effects of particle size, temperature, flow rate and sorbent characteristics on the H₂S removal efficiency of BOF-Slag were investigated. Experimental results showed that the removal efficiency of H₂S was optimum when the temperature was about 700℃ and the smaller sample size, the better sorbent capacity had and the sample size in the range of 0.214∼0.631mm didn't influence. When the temperature was above 800℃, the reactivity of sorbent has lowered because agglomeration of sorbent increased intraparticle transport resistance.

장기간 체중부하 운동훈련이 흰쥐 노화 심근에 미치는 영향

조현국(Hyung Gug Cho),이영실(Young Sil Lee),정형재(Hyeung Jae Chung),이용덕(Yong Deok Lee),박원학(Won-Hark Park) 대한의생명과학회 1998 Biomedical Science Letters Vol.4 No.2

장기간 체중부하운동아 흰쥐의 심근에 미치는 효과를 규명하기 위하여 생후 3개월, 10개월 및 20개월된 흰쥐를 5개월간 운동 시킨 후 각각의 비운동군과 비교하여 심근의 조직학적ㆍ세포학적 변화를 관찰하고, 심근세포내 미세구조적 변화를 입체해석학적으로 비교ㆍ분석하였다. 운동 15개월군부터 노화색소의 양이 현저히 증가되었고 사립체의 변성, 공포화 현상 그리고 간질조직의 증식이 뚜렷하였으며, 25개월군에서는 윤반분리, 근원섬유의 소실 및 과수축대 출현빈도가 증가되었다. Glucose-6-phosphatase의 활성도는 15개월 운동군에서 감소하여 25개월 운동군에서는 활성도가 거의 나타나지 않았다. 업체해석학적 결과에서도 장기간 체중부하운동으로 인해 15개윌, 25개월군에서 모두 사립체와 근원섬유의 체적밀도는 감소되었고, 간질조직의 체적밀도는 증가되었다. 본 연구의 결과로 볼 때 장기간 체중부하운동은 3개월군에 있어서 심장에 미치는 역효과가 없으나 10개월군과 20개월군에서는 심근 기능강화에 역효과를 초래할 우려가 큰 것으로 판단된다. There is considerable interests in the effect of regular, vigorous exercise, in particular weight training as a possible measure in improving myocardial function. The present investigation aimed to examine possible effect of a long-term weight training program on the heart in aging rats. Male rats aged 3-, 10-, and 20-month-old were divided at random into a control (sedentary) and the exercise group. The training group was exercised for 5 days a week by 1 RM of ten times with weight training apparatus. This investigation examined the changes of the heart muscle relative to histological, ultrastructural, cytochemical, and stereo logical studies in rats. Quantity of lipofuscin pigments was clearly increased in the weight training group of 15-month-old rats, and mitochondrial degeneration, vacuolization, and interstitial proliferation were observed as well. In the weight training group of 25-month-old rats the frequency as separated intercalated discs, fraying myofibrils and hypercontraction band increased in number compared with the same 15-month-old group. From the experimental result of glucose-6-phosphatase activity, the enzyme activities decreased in the weight training group of 15-month-old rats, and more decreased in the same 25-month-old group. In stereological study, both 15-and 25-month-old training groups, mitochondrial and myofibrilar volume densities significantly decreased, whereas interstitial volume density significantly increased. From the experimental results obtained in the present study, it is suggested that long-term weight training exercise do not cause any significantly qualitative and quantitative ultrastructural change of the heart muscle in the young. On the contrary, long-term weight training exercise stress may actually induce degenerative changes in the heart muscle in the old age.