재조합 Saccharomyces cerevisiae에서 Inulinase와 Invertase의 발현과 분비에 미치는 배양조건의 영향

남수완,신동하,김연희 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The effects of medium pH and culture temperature on the expression and secretion and secretion of inulinase and invertase were investigated with recombinant Saccharomyces cerevisiae cells. These cells were obtained by transformation of 2μ-based plasmids pYI10 and pYS10 which contain Kluyveromyces marxianus inulinase gene (INU1A) and S. cerevisiae invertase gene (SUC2), respectively, in the downstream of GAL1 promoter. The expression level and localization of inulinase and invertase were not affected significantly by the initial medium pH: secretion efficiencies of inulinase and invertase into the medium were about 90% and 60%, respectively, in the pH ranges of 4.0 to 6.5. However, the expression and secretion of both enzymes were strongly dependent on the culture temperature. The highest expression (7.7 units/mL) and secretion (6.7 units/mL) of inulinase were observed at 28℃ and 30℃. As a consequence of decreased localization of inulinase in the periplasmic space, the secretion efficiency increased from 68% at 20℃ to 95% at 35℃. The total expression level and secretion efficiency of invertase increased from 19 units/mL and 55% at 20℃ to 25 units/mL and 68% at 35℃, respectively. Irrespective of the culture temperature, the invertase activity in the cellular fraction (periplasmic space and cytoplasmic fractions) was kept constant at around 33∼45%.

수출용 축양품종 가지의 염절임 공정의 최적화

남학식,김남우,황성희,윤광섭,신승렬 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가지의 수출 경쟁력 향상을 위한 한 방안으로 수출용 가지품종인 축양품종을 염가공품으로 개발하기 위하여 염절임공정을 최적화하였다. 염절임 시간, 절임온도, 염농도를 독립변수로 하고 절임 후 수분함량, 염도, 표면과 내부의 색도 등을 종속변수로 하여, 중심합성계획법으로 실험을 설계하여 최적조건을 얻고자 하였다. 수립된 이차회귀모형으로 예측식을 수립할 수 있었으며 특히 수분함량이나 염도, 표면과 내부의 L과 b 값에 대하여 높은 적합도를 보여 최적조건을 수립하기 위한 제한 변수로 선정하였다. 염농도를 중심점(30%)에서 고정시켜서 얻은 절임시간과 온도는 각각 5.5~6.5일과 13-17℃였으며, 이때의 종속변수의 제한 요건으로는 수분함량 84%이하, 염도 14%이하 표면 L값 10-20, b값 0이하, 내부 L 값은 70-75, b 값이 16-18이었다. This study was conducted to the optimize salting process of eggplant for development new product and enhancement quality for export. Three variables by five level central composite design and response surface methodology were used to determine optimum conditions for salting time, temperature and salt concentration. Optimization of the process was conducted using the combination of the moisture content, salinity and color of surface and inside of salted eggplant. The regression polynomial model was suitable (P>0.05) by Lack-of-Fit analysis with highly significant. To optimize the process, based on surface response and contour plots, the individual contour plots of the response variables were superimposed. The optimum conditions for this process were 6 days and 15℃ at 30% concentration under the optimum of restricted variables as moisture content was below 84%, salinity was below 14%, L and b value of surface were 10 to 20 and below 0, L value and b value of inside were 70 to 75 and 16 to 18.

아동성폭력 예방을 위한 부모교육프로그램 개발 연구

김신연,김오남 대불대학교 2003 論文集 Vol.9 No.1

This study is to inquire into an understanding of the different kind of sexual violence of offenders the counterplan and the affter-effect of sexual violence, as well as to understand the demands on child sexual violence prevention. As a result, the goal of this study is to show the global patterns of sexual violence prevention education programs for parents which focus on models for child sexual violence prevention. The subjects were 146 parents of 4 to 7 year-old children in kindergartens located in Chonnam. Most parents thought that a child sexual violence prevention program was necessary. In the general parental understanding of sexual violence, wished body contact and others were distributed equally. Young children must have knowledge and skills to protect themselves against sexual violence and they must not have a view of the opposite sex distorted by inappropriate sexual experience.

남성·여성의 혼합DNA에서 Y-STR의 유효성 검토

이양한,신상철,이은정,박정우,구형남,김대열,김윤신 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

木浦地方의 안개發生特性

蘇鮮燮,全閏南,申弘烈 공주대학교 사범대학 과학교육연구소 1996 과학교육연구 Vol.27 No.1

안개는 수증기의 供給源인 바다, 호수, 강, 인공댐 및 태양 에너지의 입사량 변화로 發生하게 된다. 목포지방은 三面이 바다로 둘러 싸여 있으며, 주변의 造成된 영산강 댐과 2개의 人工 호수 때문에 안개 發生에 좋은 조건을 갖추고 있다. 본 硏究는 목포지방의 안개 發生 特性을 밝히기 위하여 안개 發生日數, 特續時間, 風向, 風速, 氣溫과 水溫의 偏差를 비교 分析하였다. 또한 안개의 發生 및 消散시각의 분포에 따라 4가지 Case로 분류하고 氣象要素의 變化에 따른 안개의 視程 변화를 分析해 본 결과 夏節期에는 따뜻하고 수증기를 많이 포함한 空氣가 찬 바다 위로 이동하면서 移流안개가 발생하고 봄·가을에는 氣溫의 日較差에 의해 輻射안개와 ??發안개가 複合的으로 나타난다. A fog is variously induced by the sea, the lake, the river, the artificial dam and the solar energy which originate the vapor. The Mokpo area is surrounded by the sea, the Youngsan river and the artificial lakes which naturally tend to cause a dense fog. This research shows the analogy and comparison to prove the traits of what made a fog in the Mokpo area; how many days a month the fog originates; how many hours it lasts, both the direction and the velocity of the wind, and the anomaly between atmos-pheric temperature and the sea-water temperature. The types of the fog occurrence and the dstribution of the fog-clearing time are classified into four cases. This research also tells that as a result, the change of the fogcl-earing speed brings those facts as follows: (i) when the air, warm and vaporous, is d-rifted away over the cold sea-water in the summertime; (ii) it makes the advection fog and in the season of spring and fall, it makes both the evaporation fog and the radiati-on fog at the same time, due to the great daily temperature difference. The fog in Mokpo area has high occurrence-frequency both when the velocity of the wind runs below 3 m/s and when the direction of the wind indicates NNW,NW,SSW,SSE, etc...

마스낵 제조를 위한 당절임 공정의 최적화

한주영,김남우,황성희,윤광섭,신승렬 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

대부분의 마는 건조분말제품으로 가공되고 있는데, 마를 다양한 가공품으로 개발하기 위한 방법의 하나로 삼투건조라는 전처리를 행하여 건조에 의한 품질손상을 억제하고 단맛으로 기호성을 높인 건조 마 제품을 얻을 수 있는 당절임 공정의 최적화를 시도하여 최적조건을 얻고자 하였다. 침지시간을 3-7시간, 온도는 20-60℃, 당농도는 30-70%로 하는 중심합성계획법으로 실험을 설계하여 최적화하였다. 이때의 종속변수로는 침지 후 수분함량, 당도, 색도, 그리고 동결건조후의 수분함량과 재수화율로 하여 분석한 결과 동결건조 후 수분함량에 대해서는 유의성이 없었다. 세 가지의 공정변수 중 온도의 영향이 가장 적어 온도를 중심으로 고정한 후 침지시간과 당농도의 최적조건을 찾은 결과, 수분함량을 66-70%, 당도를 25-30%, L 75이상, a-2.1-2.4, b를 5이상 그리고 재수화율을 200-250을 제한 조건으로 하는 조건으로 하는 영역은 5.2-5.9시간, 56-61%로 나타났다. This study was conducted to the optimize sugaring process of yam for development of new snack product and enhancement acceptability. Three variables by five level central composite design and response surface methodology were used to determine optimum conditions for sugaring time, temperature and concentration. Optimization of the process was conducted using the combination of the moisture content, solid content, color and rehydration ratio. The regression polynomial model was suitable (P>0.05) model by Lack-of-Fit analysis with highly significant. To optimize the process, based on surface response and contour plots, superimposing the individual contour plots for the response variables. The optimum conditions for this process were 5.5 hours and 58% at 40℃ under the optimum of restricted variables as moisture content was 66 to 70, solid content was 25 to 30%, L value was above 75, a value was -2.1 to -2.4, b value was above 5 and rehydration ratio was 200 to 250.

링형 초음파모터의 고정자 진동 해석 및 구동회로 개발

박기준,백수현,김용,윤신용,김일남 동국대학교 산업기술연구원 1999 산업기술논문집 Vol.14 No.-

본 논문에서는 링형 초음파 모터의 고정자의 굽힘진동에 대한 일반적인 해석에 대해 기술하였다. 전기-기계 결합효과를 고려하여 일반화 응력과 변형의 개념을 정립하였고, 일반화 압전방정식, 일반화 응력과 변형의 관계, 미분운동방정식에 대해 기술하였다. 또한 고정자의 진동모드 계산과 공진주파수를 계산하기 위해 유한요소법을 적용하였으며, 유한요소법을 이용한 진동해석 프로그램인 ATILA를 사용하여 고정자의 공진주파수와 변위를 계산하였다. 초음파 모터의 구동을 위해 20kHz 이상의 고주파 전원이 필요하기 때문에 2상 병렬 공진형 인버터를 제작하여 구동전압의 주파수 및 크기를 측정하여 고정자 해석과 구동회로 시뮬레이션과 비교하였다. In this paper, the bending vibration analysis of the stator for a ring type ultrasonic motor is described. Considering the electromechanical coupling effect, the concepts of generalized stress and strain are explained in detail using the generalized piezoelectric equations, which is the relationship between generalized stress and strain, the differential motion equation were derived. The vibration modes and resonance frequencies of the stator were calculated using the finite element code ATILA. For driving of ultrasonic motor, a two-phase parallel-resonant inverter was made. The stator analysis results and driving circuit simulation results are compared with measured frequencies and amplitudes.

골수염으로 발현된 재발성 콕시디오이데스 진균증 1예

백지현,박은영,정윤숙,홍재원,채윤태,진성준,최희경,신소연,한상훈,진범식,김창오,최준용,송영구,조남훈,김준명 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Coccidioidomycosis is a fungal infection that results from inhaling the airborne arthroconidia of the Coccidioides species. It is an endemic disease in the southwest part of North America and rarely diagnosed in Korea. As tourism to endemic areas and the number of immunocompromised patients have been increasing, the incidence of this infection has increased in non-endemic areas. Treatment is usually successful with antifungal agents; however, recurrence is common. It is difficult to decide when to discontinue the antifungal treatment especially in non-endemic areas where doctors are not familiar with the disease. We report a case of recurrent coccidioidomycosis manifesting as osteomyelitis after the treatment of the patient for disseminated coccidioidal infection. The complement fixation test was a useful tool for the assessment of patient response and to evaluate suspected recurrence.

Characterization of Staphylococcal Cassette Chromosome mec (SCCmec) Complex Integrated in Methicillin Resistant Staphylococcus aureus (MRSA) Isolates in Korea.

Nam-hoon Kwon,Kun-taek Park,Jin-san Moon,Hwa-young Youn,Yyoun-hee Lee,Jun-man Kim,Ji-youn Lim,Woo-kyoung Jung,Soon-keun Hong,Ji-youn Koo,Jin Hur,Sook Shin,Yong-ho Park 대한수의학회 2003 대한수의학회 학술대회발표집 Vol.2003 No.-

Analysis of Physical activity in Korean Elderly Women (With Health Center Program as the Central Figure)

Youn Shin Nam 한국여성체육학회 2012 한국여성체육학회 학술세미나자료집 Vol.2012 No.2