Insectisidal activity and feeding behavior of green peach aphid, Myzus persicae against bio-nano type of Pyrifluqinazon

Kang, Min Ah,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Shin Hyuk Jo,Shin Hyo Seob,Sae Hee Kim,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

데이터시각화와 시각적분석의 사용자 경험적 접근

강연아(Youn-ah Kang) 한국정보과학회 2016 정보과학회지 Vol.34 No.11

Molecular Cloning of Prophenoloxidase Genes from the Multicolored Asian Lady Bird Beetle, Harmonia axyridis

Min Ah Kang,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

The multicolored Asian ladybird beetle, Harmonia axyridis, is a generalist predator of aphids also, shows a high level of phenotype polymorphism in color pattern of elytra. Although, it is not sure about genetic information of color polymorphism, it has been confirmed that this phenomenon comes from their genetic traits. The color of H. axyridis elytra is mainly composed of black and red pigment. Phenoloxidase (PO) plays an important role in many insect physiological functions, i.e. sclerotization and pigmentation of cuticle and melanization of parasites. Following activation, PO catalyses the hydroxylation of tyrosine and subsequent oxidation of phenolic substance into quinines, which are further converted to melanin. However, the molecular bases of H. axyridis color pattern formation are almost unknown but it may be that the different pro-POs have different expression. In this study, total RNA samples from four each color pattern individuals, for example, succinea 1, succinea 2, conspicua and spectabilis was extracted. A cDNA enconding pro-PO was molecular cloned from each color pattern of H. axyridis and its putative amino acid sequence shared homology with pro-PO of other insects. We are pursuing to elucidate that their pro-PO sequence will be similar with those other insect PPO sequence. There are also regions of high sequence similarity, including putative activation site and two copper binding sites.

External Morphology and Distribution of Antennal Sensilla of Aphidius colemani

Hye Ri. Kwon,Min Ah Kang,Kyu Sik Yoon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,565.60± 194.64㎛) are longer than females (1,303.83±156.41㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely ten types of sensilla. We classified 3 types of sensilla trichodea as without pores, with a tip pore and with wall pores, 3 types of sensilla coeloconica, 1 sensilla placodea and 1 Bohm bristles. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception (Bohm bristles and sensilla coeloconica) and olfaction (sensilla trichodea and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.

External Morphology and Distribution of Antennal Sensilla of Aphidius colemani (Hymenoptera: Braconidae)

Kwon Hye Ri,Kyu Sik Yoon,Min Ah Kang,Min Woo Park,Shin Hyuk Jo,Shin Hyo Seob,Sae Hee Kim,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,515.20±116.48 ㎛) are longer than females (1,275.06±116.42㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely seven types of sensilla. We classified sensilla placodea, Bohm bristles, 2 types of sensilla coeloconica, , 2 types of sensilla basiconica as with a tip pore and with wall pores, sensilla trichodea. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception(Bohm bristles, sensilla coeloconicaⅡ and sensilla trichodea) and chemoreception(sensilla coeloconicaⅠ, sensilla basiconicaⅠ,Ⅱ and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.

복림이: 고령자들의 복지 정보 접근성 향상을 위한 카카오톡 기반 챗봇 디자인 컨셉 제안

한우정(Ujeong Han),유영재(Youngjae Yoo),박소연(Sohyun Park),김소연(Youn-ah Kang),강연아(Youn-ah Kang) 한국HCI학회 2021 한국HCI학회 학술대회 Vol.2021 No.1

고령 인구가 증가함에 따라 노인 복지에 대한 필요성이 증대되었다. 이에 따라 정부에서도 수요에 발맞춰 노인복지 예산을 증대하고 신규 정책을 수립하고 있다. 하지만, 복지를 위한 투자 비용 대비 국민의 복지체감도와 그 효과는 미미한 것으로 나타났다. 이러한 원인에는 복지 서비스 전달 체계의 부족이 꼽힌다. 본 논문에서는 이를 해결하기 위한 방안으로 카카오톡 챗봇 기반 복지정보 전달 서비스를 제안한다. 이를 위해 먼저 카카오톡에서 고령자에게 정보를 제공하는 방법과 챗봇의 대화 방식을 디자인하였다. 본 서비스를 통해 고령자의 복지 정보접근성을 보장하고 복지체감도를 향상시키고자 한다.

Inhibitory Effect of Undaria pinnatifida, Petalonia binghamiae and Punctaria latifolia Extracts on the Growth of Cancer Cells

Kang-Sik Choo,Hae-Nim Lee,Seong-Ah Shin,Ji-Youn Jung 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

Environmental Enrichment Enhance Functional Recovery after Focal Cerebral Ischemia through Modulation of Cyclophillin a Expression

Youn Jung Kim,Kyoung Ah Kang,Sang Sook Han 한국간호과학회 2007 한국간호과학회 학술대회 Vol.2007 No.11

Slitrk Missense Mutations Associated with Neuropsychiatric Disorders Distinctively Impair Slitrk Trafficking and Synapse Formation

Kang, Hyeyeon,Han, Kyung Ah,Won, Seoung Youn,Kim, Ho Min,Lee, Young-Ho,Ko, Jaewon,Um, Ji Won Frontiers Media S.A. 2016 Frontiers in molecular neuroscience Vol.9 No.-

<P>Slit- and Trk-like (Slitrks) are a six-member family of synapse organizers that control excitatory and inhibitory synapse formation by forming <I>trans</I>-synaptic adhesions with LAR receptor protein tyrosine phosphatases (PTPs). Intriguingly, genetic mutations of Slitrks have been associated with a multitude of neuropsychiatric disorders. However, nothing is known about the neuronal and synaptic consequences of these mutations. Here, we report the structural and functional effects on synapses of various rare <I>de novo</I> mutations identified in patients with schizophrenia or Tourette syndrome. A number of single amino acid substitutions in Slitrk1 (N400I or T418S) or Slitrk4 (V206I or I578V) reduced their surface expression levels. These substitutions impaired glycosylation of Slitrks expressed in HEK293T cells, caused retention of Slitrks in the endoplasmic reticulum and <I>cis</I>-Golgi compartment in COS-7 cells and neurons, and abolished Slitrk binding to PTPδ. Furthermore, these substitutions eliminated the synapse-inducing activity of Slitrks, abolishing their functional effects on synapse density in cultured neurons. Strikingly, a valine-to-methionine mutation in Slitrk2 (V89M) compromised synapse formation activity in cultured neuron, without affecting surface transport, expression, or synapse-inducing activity in coculture assays. Similar deleterious effects were observed upon introduction of the corresponding valine-to-methionine mutation into Slitrk1 (V85M), suggesting that this conserved valine residue plays a key role in maintaining the synaptic functions of Slitrks. Collectively, these data indicate that inactivation of distinct cellular mechanisms caused by specific Slitrk dysfunctions may underlie Slitrk-associated neuropsychiatric disorders in humans, and provide a robust cellular readout for the development of knowledge-based therapies.</P>

The Effects of Controlling the Aphis gossypii Glover (Homoptera, Aphididae) on Cucunber of Entomopahtogenic Fungus, Beauveria bassiana

Kang, Min Ah,Youn, Young Nam 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.2

Beauveria bassiana can be used as a biological insecticide to control a number of pests. It has been known as that B. bassiana TBI-1 have a insecticidal effect on the twospotted spider mite (Teranychus urticae) and the greenhouse whitefly (Trialeurodes vaporariorum) these days. The biological pest control agent, B. bassiana, showed somewhat insecticidal activities against the cotton aphid, Aphis gossypii, too. After treatment with biological insecticide, the mean of increasing rate of aphids was at around recorded at around 0.21 while at around 0.24 in non-treated TBI-1 biological insecticide condition during 1 week. Consequently, it was found that treatment of Beauveria bassiana reduced an increasing rate of aphid population. Also, we could visually observe dead aphids changed as a reddish mold due to this insecticidal fungus. B. bassiana TBI-1 can significantly reduce an increasing rate of aphid population, so effective biological control against the cotton aphid are possible. 곤충병원성 곰팡이인 Beauveria bassiana는 미생물 살충제로서 다양한 해충들을 방제하는데 사용해오고 있다. 최근에 B. bassiana TBI-1균주가 두점박이응애, 온실가루이에 살충효과가 있는 것으로 보고되었다. 생물학적 방제 인자인 B. bassiana TBI-1를 목화진딧물에도 어느 정도의 살충활성을 나타내었다. B. bassiana TBI-1 균주를 처리한 후 일주일 동안 평균 진딧물의 증가율은 0.21로 나타났으나 처리하지 않은 대조군에서는 0.24의 증가율로 약간 높게 나타나 보였다. 이러한 결과에서 백강균의 처리구에서 진딧물의 평가 증가율이 0.03 낮게 나타나는 것을 알 수 있었다. 또한 오이 잎에 B. bassiana TBI-1 균주에 의하여 붉은빛 곰팡이의 형태로 죽은 진딧물의 죽은 흔적을 육안으로 확인할 수 있었다. 적정농도로 B. bassiana TBI-1을 처리하면 목화진딧물의 개체군 증가율을 현저히 감소시킴으로 효과적으로 진딧물을 방제할 수 있을 것이다.