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MATHEMATICAL MODELING FOR THE ESTIMATION OF LIPASE ACTIVITY BY AGAR DIFFUSION METHOD
Koo, Yoon Mo,Chang, Woo Jin,Moon, Yoon Hee 한국화학공학회 1997 Korean Journal of Chemical Engineering Vol.14 No.3
A mathematical model was developed to estimate the lipase activities from the experimentally obtained physical dimension of halo, formed by a diffusing lipase through agar media. A diffusion equation based upon the mass balance was derived in cylindrical coordinates, the integration of which was carried out by using finite difference method. A regressional analysis of the experimental data using the proposed model has brought the values for the hindered diffusivity of lipase through agar media and the minimum effective lipase concentration of 0.817 x 10^(-6) ㎠/s and 2.86 unit/㎤, respectively. The procedures, delineated in this work are considered to be useful for the quantitative analysis of haloforming enzymes at predetermined standard conditions.
Controlled Lysis of Escherichia coli Double - Lysogen of Bacteriophages λHL1 and φ434
Koo, Yoon Mo,Lim, Henry C,Parekh, Bhavin S,Hatfield, G Wesley 한국화학공학회 1996 NICE Vol.14 No.3
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and, disruption. Construction of the doutrle-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. Thc single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Ø434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induce the double-lysogen to producx a protein and to lyse the host cell. The first phage gercome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cles, lacZ and defective Q genes was induced by increasing temperature to produce p-galactosidase, an intracellular reporter proteitc. The overproduction of 3-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second praphage from the lysogen MS21/Ø434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increasev of the extracxllular activity of galactosidase at the same time.
CONTROLLED LYSIS OF ESCHERICHIA COLI DOUBLE - LYSOGEN OF BACTERIOPHAGES λHL1 AND φ434
Koo, Yoon Mo,Parekh, Bhavin S,Hatfield, G Wesley,Lim, Henry C 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.2
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and disruption. Construction of the double-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. The single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Φ434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induice the double-lysogen to produce a protein and to lyse the host cell. The first phage genome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cI_(857), lacZ and defective Q genes was induced by increasing temperature to produce β-galactosidase, an intracellular reporter protein. The overproduction of β-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second prophage from the lysogen MS21/Φ434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increase of the extracellular activity of β-galactosidase at the same time.
ANALYSIS OF A NONLINEAR , COUPLED CHROMATOGRAPHIC SYSTEM
Yoon Mo Koo,Juan Hong 한국화학공학회 1994 Korean Journal of Chemical Engineering Vol.11 No.4
A relatively simple mathematical derivation is employed to obtain the relationship between the solute concentrations and their velocities along the column. These equations are used to calculate numerically the breakthrough curves and delineate the elution behaviors of competing solutes in the chromatographic column. A hodograph constructed from the derived equations using published data for the coupled adsorption isotherms is adopted to visualize the concepts of the shock and the diffusive waves. The resulting elution curves at different column lengths show a successful application of the scaling relationship between the column length and feed period to the nonlinear coupled system.
Koo, Ja-Choon,Chun, Hyun-Jin,Park, Hyeong-Cheol,Kim, Min-Chul,Koo, Yoon-Duck,Koo, Seong-Cheol,Ok, Hyun-Mi,Park, Soo-Jeong,Lee, Sung-Ho,Yun, Dae-Jin,Lim, Chae-Oh,Bahk, Jeong-Dong,Lee, Sang-Yeol,Cho, Mo Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated pnAMP-h1 and pnAMP-h2, and the corresponding genomic clones encoding these protein suggests that the peptides are produced as a prepropeptide consisting of and N-terminal signal peptide, the mature protein and C-terminal domains. The transcripts of the two genes are accumulated seed-specifically, and the maximum transcripts are observed in the mid-to-late stage of seed development. Constitutive over-expression of the pnAMP-h2 cDNA in transgenic tobacco under the control of the cauliflower mosaic virus 35S promoter conferred enhanced resistance against the oomycete Phytophthora parasitica, the causal agent of black shank disease. Thus the Pn-AMPs may play a role in the protection of seeds and may