Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

중추신경계에 침범한 속립성 결핵 : 증례보고

이태환,김헌주,허철,한용표,홍순기,변진수,김명순,조미연,김윤미 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.11

in recent times, central nervous system(CNS) tuberculosis has been rare and the prevalence of the focal form, the tuberculoma, varies from 1 per 200 to 1 per 1000. CNS tuberculosis occurs as a result of hematogenous spread from a primary focus. mostly pulmonary tuberculosis. It can be diffuse exudative leptomeningitis or a localized tuberculoma ; the former is more common. We report a case of miliary cerebral tuberculosis in a 24-year-old female who had been having headaches for 4 months. Magnetic resonance imaging showed numerous small round Gadolinium-enhanced supratentorial and infratentorial lesions scattered throughout the brain. Histologic examination confirmed well defined tuberculous granulomas with central caseous necrosis in open biopsy, containing several acid-fast bacilli, the patient was treated with isoniazid, rifampin, ethambutol, pyrazinamide in combination with prednisolone.

알콜중독자 부부집단 프로그램에 관한 연구 : For the development of a Couples Group Intervention Program for Recovery of Alcoholics(Ⅲ) 알콜중독자의 회복을 위한 부부집단 개입 프로그램 개발 연구(Ⅲ)

윤명숙,김미혜,엄예선,이은주 한국 정신보건 사회사업학회 1995 정신보건과 사회사업 Vol.2 No.-

Couple therapy has been recognized as an effective way to treat alcoholism. However, research by the authors indicate there are no couple therapy porgrams currently in the Korean psychiatric setting. The purpose of this study is to develop an effective program to aid in the recovery alcoholics and their spouses. This program is specifically designed for the couples of hospitalized alcoholics. The program consists of 10 sessions, the first four held with gender specific groups, the final five as co-joint couple sessions. The groups will meet 2 times weekly with five monthly after care follow ups. The program is divided into 3 phases ; The first phase deals with the symptoms of alcoholism and its impact on relationships with in 1st-2nd sessions. The second phase treats the issues of co-dependency and the recovery process with in 3th-4th sessions. In the final phase, conflict resolution, communication, anger control, and stress management skills are developed with in 5th-9th sessions. In the final session of the last phase, all 3 phases are integrated and summarized. At this point the group sessions are terminated, five follow up after care sessions are scheduled, to be conducted on a monthly bases. These sessions are designed to help the couple with unresolved issues, assist with implementation of their after care plan, and to monitor the couples progress with in a life of sobriety. The methods of implementing the program are centered on the cognitive behavioral approach, utilizing lectures, group discussion, role play and feedback sessions.

GH_3 세포(rat somatomammotropic tumor cell)에서 TRH가 Phospholipase D 활성에 미치는 효과

김동선,김태화,이창범,안유헌,윤미섭,한중수 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: GH_3 세포는 TRH에 반응하여 세포막의 수용체와 G 단백, PI-PLC, PKC를 활성시켜 성장호르몬 및 프로락틴을 분비한다고 알려져 있다. PLD는 phosphatidylcholine을 phosphatidic acid(PA)와 choline으로 가수분해하는 효소로서 세포의 증식과 호르몬 분비에 관여한다. 본 연구는 GH_3 세포에서 아직 알려지지 않은 TRH의 PLD 활성에 대한 영향을 규명하고자 하였다. 방법: GH_3 세포를 1.5×10^6씩 분주하고 [^3H] myristate로 표지한 다음에 0.3% 알코올로 전 처치하였다. TRH 등의 시험제를 처치한 후에 메탄올로 반응을 종결하고 세포에서 총 지질을 추출하였다. PLD 활성은 박층크로마토그래피를 이용하여 총 [^3H] phospholipid에서 [^3H] phosphatidylethanol의 비율로 구하였다. 결과: TRH (1μM)의 처치 시에 PLC 활성은 44배 증가하였다. PLD 활성은 TRH (1μM), mastoparan (5μM), PMA(500nM)를 30분간 처치 시에 각각 1.9, 1.5, 2.2배 증가하였다. TRH(1μM)의 시간에 따른 PLD 활성 변동은 15, 30, 60, 120, 240분에 각각 142%, 170%, 172%, 160%, 115%의 증가를 보였다. 결론: GH_3 세포에서 TRH의 호르몬 분비와 세포증식의 신호전달 기전으로서는 PLC 활성뿐 아니라 PLD의 활성도 관여함을 시사한다. Backgroud: GH_3 cells are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone (TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phospholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in cellular proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in GH_3 cells, we investigated the activities of PLC and PLD in GH_3 cells treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methos : GH_3 cells were labeled with [^3H] myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by measuring [^3H] phosphatidylethanol from the [^3H] phospholipid using thin layer chromatography. Results : TRH (1μM) stimulated the PLD activity by 44-fold over that of the control values. TRH (1μM), mastoparan (5μM), and PMA (500μM) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls, The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1μM) were 142%, 170%, 172%, 160% and 115%, respectively. Conclusion : These results suggest that TRH stimulates not only PLC activity, but also the PLD activity in GH_3 cells (J Kor Soc Endocrinol 17:465∼472, 2002).

당뇨병환자에서 게이트심장혈액풀신티그라피를 이용한 심기능 평가

윤상임,송치운,이진홍,안미애,성기양,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Major cardiovascular complications of diabetes are coronary atherosclerosis, diabetic dilated cardiomyopathy, autonomic neuropathy and those are major causes of morbidity and mortality in diabetic patients. Gated blood pool heart scan is noninvasive and useful method for evaluation of functional status of heart in diabetics. We evaluated 52 patients with diabetes and divided 3 groups. Group 1 were 11 patients without proteinuria or with proteinuria less than 550mng during 24 hours. Group 2 were 9 patients with proteinuria more than 550mg during 24 hours and group 3 were 32 patients with endstage renal diasese due to diabetes. We performed 99mTc-HSA cardiac gated blood pool scan and used left ventricular ejection fraction(LVEF), peak ejection rate(PEF) to indices of LV systolic function and peak filling rate(PER) to index of LV diastolic function. The results were follows : 1) LVEF, PER were significantly lower in diabetics with ESRD than diabetics without ESRD, but there were no significant difference between normal controls and diabetics without ESRD 2) PFR was significantly lower in diabetics than normal controls, but there were no significant differences in diabetics with or without nephropathy. 3) There were negative correlation between PER, PFR and duration of diabetes. On the basis of results, PFR is a LV functional index of GBPS which can disclose early change of LV dysfunction in patients with diabetes.

석유화학 공정의 가상사고 시나리오 유형분석

윤동현,강미진,이영순,김창은 한국안전학회 2003 한국안전학회지 Vol.18 No.3

This paper presents a set of typical pseudo-accident scenarios related to major equipments in petrochemical plants, which would be useful for performing such quantitative risk analysis techniques as fault tree analysis, event tree analysis, etc. These typical scenarios address what the main hazard of each equipment might be and how the accident might develop from an "initiating event". The proposed set of accident scenarios consists of total thirteen (13) scenarios specific for five (5) major equipments like reactor, distillation column, etc., and has been determined and screened out of one hundred and twenty-five (125) potential accident scenarios that were generated by performing semi-quantitative risk analysis practically for twenty-five (25) petrochemical processes, considering advices from the operation experts. It is assumed that with simple consideration or incorporation of plant-specific conditions only, the proposed accident scenarios could be easily reorganized or adapted for the relevant process with less time and labor by the safety engineers concerned in the petrochemical industries.

두부회전에 따른 측모두부방사선 계측치의 변화

김광수,황미선,최의환,김광원,윤영주 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.1

본 연구는 측모두부방사선사진 촬영시 발생될 수 있는 두부회전이 측정된 선, 각계측치들에 어느 정도의 투사오차를 야기시키는지 알아보기 위해 조선대학교 의과대학 해부학교실에 소장중인 건조두개골 중 비교적 상태가 양호하고 특별한 비대칭이 없는 영구치열기의 건조두개골 17개를 표본으로 선택하여 시행하였다. 각각의 건조두개골을 수직축(Z축)을 중심으로 기준위치(0˚)에 대해 1˚ 간격으로 ±15˚ 까지 실험적으로 회전시켜 총 527장의 측모두부방사선사진을 촬영하였다. 이를 근거로 기준위치(0˚)에서의 계측치와 각 회전각에서의 계측치들 사이에 paired t-test를 시행하여 측모두부방사선사진 계측치 간의 차이를 규명하였으며, 이를 통해 투사오차의 관점에서 교정학적으로 유용한 측모두부방사선 계측항목을 구한 결과 다음과 같은 결론을 얻었다. 1.각계측항목이 선계측항목에 비해 투사오차가 작았다. 2.각계측항목은 정중시상면에 위치한 기준점들을 많이 포함할수록 투사오차가 작았다. 3.수평선계측항목의 길이는 필름방향으로 회전됨에 따라 점진적으로 감소되었으나, 초점 방향으로 회전됨에 따라서는 증가되다가 감소되었으며 상대적으로 그 변화양이 작았다. 4.두부회전에 따른 투사오차는 수직선계측항목에 비해 수평선계측항목에서 컸다. 5.수직선계측항목은 회전축으로부터 거리가 증가함에 따라 투사오차가 증가하였다. 이상을 종합해 볼 때 두부회전에 따른 측모두부방사선사진 계측치의 투사오차를 최소로 하기 위해서는 선계측항목보다는 각계측항목을 사용하는 것이 유용할 것으로 사료된다. This study was performed to find out the effect of projection errors on cephalometric linear and angular measurements according to head rotation during taking lateral cephalometric radiographs. Seventeen skulls with permanent dentition and no gross asymmetry were obtained from the Department of Anatomy, Medical School, Chosun University. Total 527 x-ray films were taken with 1˚ interval from the reference position(0˚) to ±15˚ around the vertical axis (Z axis) which is perpendicular to the midpoint of the line connecting the center of two ear rods in submento-vertex direction. Statistical analysis was performed by faired t-test if there were statistically significant differences between the mean of the reference position(0") and that of each rotation angle. The following results were obtained. 1.The projection errors of angular measurements were smaller than those of linear measurements. 2.The projection errors of angular measurements including midline landmarks were smaller than those including bilateral landmarks. 3.The horizontal linear measurements were gradually decreased when the skull was rotated toward the film, but slightly increased and then decreased when the skull was rotated toward the focal spot. However, the changes were smaller in focal direction. 4.The projection errors of horizontal linear measurements were larger than those of vertical linear measurements. 5.The projection errors of vertical linear measurements were increased with increased distance from the rotation axis to vertical measurements. It is concluded that the use of angular measeurements rather than linear measurements is recommended to minimize the projection errors.

일 대학 병원 마취 · 회복실 간호사의 간호활동 분석

강윤경,김경미,김연이,박혜옥,서광희,송숙녀,이현숙,조의영 대한간호행정학회 2006 간호행정학회지 Vol.12 No.1

Purpose: This study was aimed to specify roles of nurses from the anesthesia and recovery room by analyzing nursing activities as well as anesthesia nursing during surgery. Method: The objects were 12 RNs working in the recovery room of a university hospital located in Incheon. Self-report was performed by measuring stop-watch for five days from May 17th to May 21st in 2004. Research method was designed to record the time and frequency of the nursing activity from anesthesia and recovery room based on nurses' statement and other references for five days. The data were analyzed with descriptive statistics, ANOVA, and t-test via SPSS Win 10.0 program. Result: Nursing activities in anesthesia recovery room were classified into two different characteristics such as direct and indirect nursing activity. The activities consisted of 11 direct nursing areas and 39 nursing activities in anesthesia preparation room. The indirect nursing was classified into 8 nursing areas and 32 nursing activities. The direct nursing was classified into 12 nursing areas and 55 nursing activity. Also, the indirect nursing was classified into 7 nursing areas and 21 nursing activities in recovery room. In terms of prevalence of nursing activities in the anesthesia preparation room, observation and cooperation of anesthesia was the most prevalent activity, drug and eqipment management the second prevalent, and drug administration the third. On the other hand, in the recovery room, the most prevalent activity was vital sign checking and observation, the second most prevalent activity informative activity, and the third body temperature control. Nursing activity time was recorded according to the nursing characteristics. In the anesthesia preparation room, the direct nursing spent for 8092.20 minutes was larger than the indirect nursing spent for 7198.50 minutes. Also, in the recovery room, the direct nursing spent for 2361.16 minutes was larger than the indirect nursing spent for 1134.13 minutes. 4. Nursing activity time was compared to duty shifts. In the anesthesia preparation room, the direct nursing was more prevalent on day shift and the indirect nursing was prevalent on evening shift. However, in the recovery room, both direct and indirect nursing activities were prevalent on day shift. Conclusion: The role of anesthesia and recovery room nurses was analysed according to the time, frequency, and its characteristics.

임신성 영양모세포 질환에서 bcl-2 및 p53 단백 발현의 상관성에 관한 면역조직화학적 연구

조미연,송지선,김윤미,이상엽,정순희,이광길 연세대학교 원주의과대학 1999 원주의대논문집 Vol.12 No.1

경련유발자극이 흰쥐 뇌에서 heat shock protein72 유전자발현에 미치는 영향

김현,김창미,강윤희,유임주,박재현,서영석 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4