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Effects of Vancomycin, Roxithromycin, and Rifampin on Biofilm Formed by Staphylococcus epidermidis
Lim, Young-Seong,Kim, Yang-Ree,Choi, Jung-Hyun,Yoo, In-Hong,Shin, Wan-Shik,Kang, Moon-Won 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-
Background : Catheter-associated peritonitis occurs frequently in patients with chronic renal failure with peritoneal dialysis. It can be controlled by antibiotics, but relapses are common due to biofilms that affect the pathogenesis of peritonitis. This study was designed to evaluate the efficacy of antibiotics against biofilm formed by Staphylococcus epidermidis which is the most common causative organism of peritonitis in patients with periodontal dialysis. Methods : After incubation of silastic plates for 24 hours in peritoneal dialysis fluid containing S. epidermidis, the establishment of bacterial biofilm on the plates was establishment of bacterial biofilm on the plates was confirmed by scanning electron microscopy. Then the plates were incubated in dialysate containing various concentrations of antimicrobial agents, such as vancomycin, roxithromycin or rifampin, in combination or alone. The quantification or organisms on silastic plates was performed for evaluation of antibiotic efficacy against biofilm formed by S. epidermidis after getting samples of plates at 0 hour, 4, 24, and 48 hours after incubation. Results : The reduction of colony counts in groups with antibiotics was significantly lager than that in the control group and the difference was increased as time passed. In vacomycin treated plates, the colony count per plate for 2×MIC (minimal inhibitory concentration) was significantly lower than that for 1/8×MIC or 1/64×MIC at 4 hour, but no significant differences were noted at 24 hours and 48 hours among those of the various concentrations. The reduction of colony counts in accordance with concentrations of roxithromycin was not significantly different at 4 hours, but the reduction for 1/8×MIC was larger than that for 1/64×MIC at 24 hours and 48 hours. There was no significant difference in reduction of colony counts between groups of antibiotic combination containing vancomycin and groups of vancomycin alone. Conclusions : It is suggested that the sub-inhibitory concentrations of vancomycin, roxithromycin, and rifampin might be effective on biofilm-forming S. epidermidis in in vitro model of infection related with peritoneal dialysis catheter. This result may be applicable for development of a new method to control biofilm-associated peritonitis during peritoneal dialysis. (Korean Journal of Infectious Disease 31:317-324, 1999)
문유선,임기영,김도훈,이혜리,유계준,오병훈,민경준 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.6
This study was designed to determine the frequency and characteristics of physical symptoms and diseases in the psychiatric inpatients, and was directed to assess the physical problems and possible ways to resolve them in a closed psychiatric unit. Through evaluation of medical records of 386 inpatients from Kwangju Severance Psychiatric Hospital of Yonsei Medical College during a one-year peroid, from September 1, 1993 to August 31, 1994, we classified the physical problem according to ICD-9CM, comparing with the contents of primary health care in care in family practice. The results were as follows ; Among the total number of 386 psychiatric inpatients, 159 patients(41.2%) had the physical problems with 196 physical diagnoses(1.23 diagnoses per a patient). The most common diagnoses were constipation, upper respiratory tract infection, tinea, alcholic liver disease, dental caries in a descending order. The most common systemic diagnoses were diseases of digestive system, skin, ENT(ear, nose and throat)and dental diseases. The 81.6% of the physical problesm were consulted to a family physician with referral rate of 5.3%. Our results suggest that the majority of the physicial problems in a closed psychiatric unit could be resolved by consulting to a family physician.
수소화물 발생-유도결합 플라스마 원자 방출 분광법을 이용한 폐광산 광미 시료 중의 As(III)와 As(V)의 동시 정량
김선태,임유리,박경수,정진호,Kim, Sun-Tae,Lim, Yoo-Ree,Park, Kyung-Su,Chung, Jin-Ho 한국분석과학회 2000 분석과학 Vol.13 No.2
수소화물 발생-유도 결합 플라스마 원자 방출법(HG-lCP-AES)을 이용하여 폐광산 광미 시료중의 As(III)와 As(V)를 동시 정량하였다. 폐기물 공정 시험법에 준하여 용출한 폐광간 광미 시료 중에서 전체 비소는 1M KI를 사용하여 As(V)를 As(III)로 예비 환원 후 2% $NaBH_4$와 6M HCl로 분석하였고, As(III)는 pH 5-6의 citrate/citric acid 완충 용액을 사용하여 선택적으로 정량한 후 전체 비소의 양에서 As(III)의 양을 보정하여 As(V)를 정량하였다. 그 결과 폐광간 광미 시료에서 용출된 전제 비소 중에서 As(V)의 함량이 90% 이상이었다. Arsenic (III) and arsenic (V) in disused mine tailing samples have been determined simutaneuously by hydride generation inductively coupled plasma atomic emission spectrometry (HG-ICP-AES). Total arsenic was determined using 2% $NaBH_4$ and 6 M HCl after prereduction of As(V) to As(III) with) 1M KI. Arsenic (III) was determined selectively using citrate/citric acid buffer with range of pH 5-6, it was determined by HG-ICP-AES. Arsenic (V) can be evaluated by the differences. According to the results, arsenic (V) was over 90% among the total arsenic extracted from disused mine tailing samples.
페로니켈 슬래그로부터 Mg 이온의 용출특성과 화합물 제조
추용식,임유리,박홍범,송훈,이종규,이승호,Chu, Yong-Sik,Lim, Yoo-Ree,Park, Hong-Bum,Song, Hun,Lee, Jong-Kyu,Lee, Seung-Ho 한국세라믹학회 2010 한국세라믹학회지 Vol.47 No.6
Ferro-Nickel slag is one of the by-products in Ferro-Nickel manufacturing process. The slag is composed of $SiO_2$, MgO, $Fe_2O_3$ and others. But the slag has been buried at landfill despite having valuable elements. This study tried to extract Mg ion and fabricate Mg compound from ferro-nickel slag using hydrochloric acid solution. Mg ion was extracted with Si, Fe and other ions in HCl solution. So reprocess was needed for gaining high purity Mg ion. It was thought that Si ion or $SiO_2$ precipitated in HCl solution and removed from solution in filtering process. Fe ion converted into $Fe(OH)_3$ after reacted with $NH_4OH$ and precipitated in HCl solution. After these process, the filtrate was composed of high purity Mg ion. $MgCl_2{\cdot}NH_4Cl{\cdot}6H_2O$ was obtained through drying of filtrate and this product was changed into MgO by burning process ($600^{\circ}C$-30 min). That is, 1st material or solution for manufacturing 2nd product was fabricated using acid dissolution method and other treatments.
( Jong-kil Seo ),( Ji Hwoon Baek ),( So Jeong Kim ),( Ja Hyun Ryu ),( Min Ji Kim ),( Da Jung Jo ),( Young Kyoung Seo ),( Han Jo Kim ),( Yoo Ree Lim ),( Jong Hyun Kim ),( Sung Min Yang ),( Hye-jin Ahn 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2
Background: Despite many reports on the skin aging factors using non-invasive methods, there is a need to identify reliable skin aging factors obtained in sufficient subjects and in a properly controlled environment. Objectives: To investigate the characteristics of facial skin of eight Asian countries and to confirm the aging parameters of 20- to 50-year-old Asians. Methods: The subjects consisted of 5,205 healthy males and females ranged from 20 to 59 years-old in South Korea, Mongolia, Taiwan, Kazakhstan, Russia, Turkey, the Philippines, and China. Skin hydration, transepidermal water loss (TEWL), pore area, pH, elasticity, wrinkles, and skin color on the face were measured under the environmental conditions of specific relative temperature and humidity, controlled and maintained identically for each volunteer. Results: Elasticity, pore area, wrinkles, skin color, and TEWL on the face were correlated with age, but skin hydration and pH were not correlated with age. Elasticity, pore, and wrinkles parameters showed higher r-values than others. The r-value of elasticity was -0.740 in R2, -0.436 in R5, and -0.539 in R7 (p <0.01). The r-value of pores and wrinkles were 0.523 and 0.502, respectively. Conclusion: The present study suggests that the aging parameters of the facial skin that are highly correlated with early aging in 5,205 subjects are elasticity, area of pores, and wrinkles.
서영경 ( Young Kyoung Seo ),김민지 ( Minji Kim ),김소정 ( So Jeong Kim ),백지훈 ( Ji Hwoon Baek ),고재숙 ( Jae Sook Koh ),양성민 ( Sung Min Yang ),김종현 ( Jong Hyun Kim ),임유리 ( Yoo Ree Lim ),최성원 ( Sung Won Choi ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.3
오늘날의 사회에서는 삶의 질 향상으로 실제 나이보다 젊어 보이며 이에 따라 노화방지에 대한 관심이 증가하고 있다. 또한 같은 나이의 사람들과 비교했을 때 본인이 젊어 보이는지에 대한 관심이 높아지고 있다. 이 연구에서 우리는 외적 노화에 가장 주요한 영향을 주는 주름 지수를 구하고자 하였다. 전체 얼굴의 주름은 8개로 분할된 영역에 의해 점수화되었고, 실제 연령과 피부 파라미터 사이의 상관 관계를 분석하였다. 연구 대상자 206명(한국인 여성 105명, 몽골 여성 101명)을 대상으로 하였다. 대상자는 연령대별로 20대, 30대, 40대, 50대를 네 군으로 나누었다. 주름 패턴은 주름, 미간, 코뿌리, 눈꺼풀, 눈밑주름, 눈꼬리, 팔자주름, 입가 8개 부위의 주름을 평가하고 주름 점수에 따른 계산식을 개발 하였다. 또한 계산식에 의해 얻어진 주름지수와 연관성 있는 피부 특성 파라미터를 알아보고자 피부 탄력, 모공, 주름, 피지 분비를 측정하였다. 방정식을 적용하여 계산한 한국인의 경우 주름연령과 실제연령과의 차이는 없었다. 반면, 몽골인에서는 실제연령과 비교하여 9세가 더 나이 들어 보인다고 밝혀졌다. 얼굴 주름 나이와 피부 특성 파라미터 사이의 상관 관계는 양국에서 피부 탄력 > 모공 또는 눈꼬리 주름 > 피부 색 > 피지 분비 순으로 나타났다. 피부 탄력은 얼굴 주름 나이와 가장 관련이 있는 변수로 나타났다. 본 연구를 통해 한국과 몽골 여성의 연령별 피부 주름 패턴을 규명하였으며, 이 연구로부터 개발된 주름살 계산식을 향후 화장품의 효능 연구에서 얼굴 주름의 나이를 계산하는 도구로 사용할 수 있을 것으로 생각된다. In today’s society, where people look younger than their chronological age due to improvements in the quality of life, there is a gaining interest in anti-aging and how people compare to those in the same age group. We evaluated the wrinkle index, which is the most important attribute amongst skin parameters, to evaluate external age (wrinkle age). The wrinkles of the whole face were scored by divided 8 areas (forehead, glabella, nasal root, upper eyelid, lower eyelid, crow’s feet, nasolabial groove and perioral skin) and analyzed the correlation between chronological age and skin parameters. 206 subjects (Korean female, n = 105 and Mongolians female, n = 101) were enrolled. Subjects were divided into four groups by ages: 20s, 30s, 40s, and 50s. Wrinkle scores of 8 areas were evaluated and developed a calculation formula based on the wrinkle scores. Skin characteristic parameters were measured about skin elasticity, pore, wrinkle, sebum secretion. There was no difference between the calculated ages and the chronological ages in Korean women. On the other hand, Mongolian looked older than chronological age by 9 years. The correlation between the facial wrinkle ages and skin physiology parameters was presented in the order of skin elasticity > pore or crow’s feet > skin tone > sebum secretion in both countries. Skin elasticity represented the most related parameter with the facial wrinkle ages. This study identified the skin wrinkle patterns of Korean and Mongolian women and the wrinkle age calculation formula developed from this study can be used as a tool for calculating the facial wrinkle ages in cosmetic studies.