Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

작약의 RAPD 분석을 위한 PCR 최적조건 구명

최인수,김성만,김용철,이충렬,박현철 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

To optimize the PCR condition is one of the most important steps for RAPD analysis. The purpose of this study was to optimize PCR condition in Paeonia. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, and extension) was also conducted. The most appropriate template DNA concentration was 60ng. Clear bands were observed from 2.5mM and 4.5mM of MgCl2 if template DNA concentration and amount of taq polymerase were proper. Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit. In the consideration of results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions (60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and lunit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

분자 배향성 제어를 이용한 유기전기발광소자 개발에 관한 연구

崔淙宣,李榕洙 弘益大學校 科學技術硏究所 2000 科學技術硏究論文集 Vol.11 No.-

The organic electroluminescent devices based on small molecules or conjugated polymers have been greater interest due to their possible applications for flat panel displays. They are attractive because of the capability of multicolor emission, low operating voltage, low power consumption, and competitive cost. The high mobilities allow larger current densities to be reached under operation in the space charge limited conduction region, hence facilitate access to higher luminance as required for passive matrix devices. In this study, we have investigated the molecular orientation control effect of organic electroluminscent devices. We aligned the p-sexiphenyl, which is a blue emitting material, using simple rubbing treatment. We obtained the anisotropic optical propery by UV/visible absorption measurement. In the current density-voltage relationship, we observed that the current density of the rubbing treated devices is larger than that of the non-rubbed device. This indicates that there is a more efficient carrier injection from the anode in the rubbed p-sexiphenyl devices.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1

대장균의 nlp(Ner like protein) 유전자를 클로닝하여 구조해석을 한 결과 전사조절 단백질인 Ner와는 61%의 높은 상동성을 가지고 있음을 이미 보고한 바 있다. 본 연구는 당 대사 관련 유전자의 발현 조절을 보고자 nlp 유전자를 도입시켜 tac promoter에 의해 대량 발현시킴으로서 lactose대사 관련 유전자 lacZ와 maltose 대사 관련 유전자인 malQ, malP의 유전자 발현이 2.5배에서 최고 8.3배 정도까지 증가됨을 확인하였다. 이는 nlp유전자가 cAMP비존재하에 crp*1와 상호작용하여 maltose및 lactose대사를 촉진시킴을 시사한다. nlp-lacZ 융합 유전자 산물을 Immunoblotting하여 분석한 결과 nlp의 promoter가 in vivo 상태에서 발현되어짐을 확인하였다. tac promoter와 IPTG에 의하여 nlp유전자 산물을 대량 발현시킨 결과 약 10,000Da의 산물을 SDS-poly-acrylamide gel 전기영동으로서 확인하였으며, 부분 정제된 Nlp단백질을 조절 영역의 DNA단편에 결합함으로서 전사조절에 관여하는 것으로 사료되어졌다. An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here we show that expression of the sugar metabolism related genes, lacZ, malQ and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose-and lactose-metabolism coordinately with crp*1 in the absence of CAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified NIp protein bound a specific DNA of the regulatory region of the nlp gene.

MPLS망에서 RSVP와 DiffServ연동모델의 성능분석

최승권,오구영,지홍일,신승수,조용환 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The explosive growth of the internet traffic has imposed tremendous stress on routers, particularly, in the core network. Today's IP services reflect the unpredictable and undifferentiated packet loss and jitter characteristics of traditional best-effort routers. For above requirements, it is required not only the high-speed transmission of the packet, but the new routing function, the guarantees of QoS and bandwidth, the supports of user-requested services MPLS is proposed by the IETF and many other research institute as the solutions for this problem. In this paper, analyze performance of QoS Model applied RSVP over DiffServ to MPLS network. this model is each RSVP applied access network and DiffServ applied core network. In Result, It is amiss that basis MPLS net uses to core network because the average packet loss rate and average delay time are increased if traffic increases. Experiment result was seldom difference in case of network that apply each RSVP, DiffServ, RSVP over DiffServ. RSVP over DiffServs case packet loss rate and delay time were low than DiffServ and the packet loss rate is high because comparing than RSVP but delay time was very low.

유자종실의 Sterol 조성

최상도,주옥수,조용운 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

남해산 柚子種實油을 分析試料로 하여 日本, 基準油脂分析試驗法에 따라서 不檢化物量을 定量하고 T. L. C法으로 不檢化物을 分劃하였으며 G. C法에 依해 sterol組成을 分析하였다. 유자종실기름의 불검화물량은 기름 1g당 19.2mg으로서 1.9%이였고 불검화물의 분획 pattern은 less polar compounds가 20.3%, 4,4-dimethylsterol이 9.6%, 4-desmethylsterol이 47.1% 및 미전개물질이 22.9%이였다. sterol조성은 cholesterol이 0.3%, campesterol이 11.9%, stigmasterol이 9.3%, β-sitosterol이 72.2% 및 未知 sterol이 6.3% 이였다. 불검화물량, 불검화물의 분획 pattern 및 sterol조성은 他植物種實油와 거의 같은 경향을 나타냈다. This experiment was performed to the unsaponifiable matters content of the citrus seed oil. Also, the fractionated sterol pattern and the sterol composition of the citrus seed oil were examined. The citrus seed oil were contained 19.2mg unsaponifiable matters per gram. The unsaponifiable matters in seed oil were fractionated into less polar compounds(20.3%), 4, 4-dimethylsterol(9.6%), 4-monomethylsterol(0%), 4-desmethylsterol(47.1%) and undeveloped matters(22.9%). The sterol composition in citrus seed oil were cholesterol 0.3%, campesterol 11.9%, stigmasterol 9.3% and beta-sitosterol 72.2%. But brassicasterol and avenasterol were not detected.

부비동 환기의 연구를 위한 인체 비부비동 모형

정용수,김상렬,김동환,정동근,최순섭,정인호,서세훈 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.9

복합재료 구조물 수리시 발생하는 잔류변형에 대한 덧대기 현상

최항석,이수용 한국항공대학교 1999 論文集 Vol.37 No.-

복합재료 구조물의 덧대기 수리시에 발생하는 잔류변형에 대한 덧대기의 영향을 파악하기 위해서 유한요소 해석을 수행하였다. 유한요쇼 프로그램은 삼차원 응축 쉘 요소와 일차 전단변형 이론에 근거하여 개발되었다. 준등방성, 직교이방성, angle-ply 적층 쉘에 대해 각각 angle-ply, 준등방성, 직교이방성 덧대기 적층으로 수리하였을 때 최종 잔류변형률을 계산하였다. 최대 잔류변형률은 대부분 적층쉘과 덧대기의 인접 부위에서 발생하였고, 그 부위에서 상당히 급격하게 변화하였다. 잔류변형은 적층쉘과 덧대기의 적층순서에 따라 크게 좌우되며, 또한 cutout 영역이 커짐에 따라 일반적으로 잔류변형이 증가하였다. Finite element analysis is performed to investigate the effect of patches on residual deformation induced by the patch repair of composite structures. The finite element program is developed based upon a 3-D degenerated shell element and the first shear deformation theory. Final residual strains are calculated for quasi-isotropic, orthotropic and angle-ply laminated shells repaired with angle-ply, quasi-isotropic and orthotropic prepreg patches, respectively. Maximum residual strains are occurred and abruptly changed near at interfaces of the laminated shell and the patch. Residual deformation is depended on stacking sequences of the laminated shell and the patch, and is generally increased as the region of a cutout is increased.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

웨이블릿 영역에서 상관함수를 이용한 3-State 블라인드 디지털 워터마크

최용수,김형중 강원대학교 정보통신연구소 2002 정보통신논문지 Vol.6 No.-

The content's authentication is very important in the field of Digital Multimedia Contents which is based on the Internet. There are several methods to perform the authentication. The Watermarking is the one of those methods. This paper's every process is based on the image application. The proposed watermarking method has the 3-State information and performs the embedding and the detection without reference Content. when we authenticate the Owner Information, it uses correlation function. The special feature is embedding the watermark depends on the original contents and using the DWT(Discrete Wavelet Transform) Domain when it is applying the SS(Spread Spectrum) method. therefore, certain extra probability of error will be reduced. and DWT is more faster than other transform method(DFT, DCT etc). When we use N size image, the computation can reduce from O(N·logN). and we can hide some more information per bit. In this experiment, We used some general signal processing (such as Noise Addition, Compression, Scaling etc.) as the Attacks.