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Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction
( Ji Young Kim ),( Yong Beom Choe ),( Kyu Joong Ahn ),( Yang Won Lee ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.3
Background: Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. Objective: To assess the value of multiplex PCR in identification of dermatophytes. Methods: Using three specially- designed primers which contained the ITS1-2, 18S rRNA, and 28S rRNA regions, three cycles of PCR were performed on 11 standard strains and scales were collected from 73 patients with fungal infection. Results: The 11 standard strains were successfully identified with analysis of band patterns of ITS1-2, 18S rRNA, and 28S rRNA, obtained from PCR. Based on this information, the causative organisms in 73 patients with fungal infection were revealed to be T. rubrum in 69 cases, T. menta in 1 case, T. tonsurans in 2 cases, and M. gypseum in one case. Conclusion: With three cycles of PCR using three sets of primers, 11 standard strains and the clinical strains from 73 patients with fungal infection were successfully identified.
( Min Seok Hur ),( Yong Beom Choe ),( Kyu Joong Ahn ),( Yang Won Lee ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.4
Background: Although oral antihistamines (H1-histamine receptor antagonists) are the main treatment option for pruritus in general skin dermatosis, their effect in treating pruritus of atopic dermatitis (AD) has not yet been established. Objective: We conducted a systematic review and meta-analysis to evaluate the effectiveness of combined therapy of H1-antihistamines and topical steroids. Methods: We systematically searched MEDLINE, Embase, and CENTRAL databases for articles published from 1967 to 2015. We identified 1,206 studies and assessed their titles, abstract, and full-text. Random effects meta-analysis was used to calculate mean differences (MD) with 95% confidence intervals (CI). Results: Two studies satisfying the inclusion criteria of antihistamine therapy with mandatory topical steroid use were selected. Comparing antihistamine monotherapy with combination therapy, patients treated with the addition of antihistamine to topical corticosteroids showed a statistically significant clinical improvement (standard MD, -0.24; 95% CI, -0.42 to -0.05; p=0.01). Conclusion: H1-antihistamines may have a synergistic effect when combined with topical steroids by influencing various associative factors of chronic pruritus in AD. (Ann Dermatol 31(4) 420∼425, 2019)