쥐에서 Phenobarbital Sodium 및 3-Methylcholanthrene이 ^14C-carbofuran의 대사에 미치는 영향

임요섭,한성수 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

쥐에서 carbofuran 대사에 미치는 phenobarbital sodium (PB) 또는 3-methylcholanthrene (3-MC)의 영향을 조사하기 위하여 쥐에 이들을 단독 또는 조합으로 경구투여한 후 일정 간격으로 쥐의 주요 장기, 대변, 소변 및 혈액 중 대사산물의 종류와 생성율을 조사하였다. Carbofuran 단독투여와 carbofuran과 PB 또는3-MC 조합투여 모두 경구투여 후 48시간 이내에 빠르게 배설되어 ^14C-carbofuran 총 투여량의 79.9∼81.1%가 소변으로, 5.7∼6.5%가 대변으로 배설되었는데, 배설속도는 carbofuran 단독투여보다 carbofuran과 PB 또는 3-MC 조합투여에서 빨랐다. 쥐의 주요 장기, 대소변 및 혈액 중의 carbofuran의 대사산물은 공통적으로 3-hydroxycarbofurun, 3-ketocarbofuran, 3-hydroxycarbofuran phenol, 3-ketocarbofuran phenol과 carbofuran phenol이었고, 주요 대사산물은 3-hyoxycarbofuran과 3-ketocarbofuran이었는데, 주요 대사산물의 경우 carbofuran만의 투여에서는3-hydroxycarbofuran이었으나 carbofuran과 PB 또는 3-MC 조합투여는 3-ketocarbofuran이었다. 소변 중 carbofuran의 2가지 주 대사산물의 생성율은 carbofuran 단독투여시 3-hydioxycarbofuran 17.4%와 3-ketocarbofuran 12.8%이었고, carbofuran과 PB 또는 3-MC 조합투여시 3-hydroxycarbofuran 8.6%와 3-ketocarbofuran 23.5%로서, carbofuran단독투여와 carbofuran과 PB 또는 3-MC 투여사이에 대사산물의종류는 같았으나 생성율에는 큰 차이가 있었다. 이와 같은 결과는 쥐에 carbofuran 투여 후 PB나 3-MC를 투여함으로써 carbofuran의 대사가 빠르게 이루어지고, 주 대사산물 중3-hydroxycarbofuran보다 독성이 낮은 3-ketocarbofuran으로의 대사가 빠르게 이루어지기 때문에 carbohran의 독성이 경감되어 쥐가 생존할 수 있는 것으로 판단된다. In order to elucidate the effect of phenobarbital sodium (PB) and 3-methylcholanthrene (3-MC) on metabolism of insecticide carbofuran in rat. Carbofuran metabolites and its formation rates were determined when orally administered ^(14)C-carbofuran alone and its combination with PB or 3-MC to rat. ^(14)C-carbofuran administered orally, alone or in combination with PB or 3-MC, was secreted rapidly within 48 hrs. That is, 79.9 to 81.1% of the original radioactivity was secreted into the urine and 5.7 to 6.5% into the feces. The secretion rate was faster in the combined administration than that in carbofuran alone. Metabolites of carbofuran in main organs, urine, feces and blood of rat were largely 3-hydroxycarbofuran, 3-ketocarbofuran, 3-hydroxycarbofuran phenol, 3-ketocarbofuran phenol, and carbofuran phenol, the major ones being 3-hydroxycarbofuran and 3-ketocarbofuran, respectively, in all administrations of carbofuran alone, carbofuran+PB and carbofuran+3-MC. In addition, formation rate of the two major metabolites detected in the urine was 17.4% and 12.8%, respectively, when carbofuran alone was administered. Meanwhile, when carbofuran was administered with PB or 3-MC, they were 8.6% and 23.5, repectively. These results indicate that the oral administration of PB or 3-MC can reduce carbofuran toxicity by fastening and stimulating the carbofuran metabolism in rat.

몇 가지 식물추출물이 배양 NIH3T3 섬유모세포의 세포생존율과 세포부착률에 미치는 세포독성에 관한 연구

임요섭 ( Yo-sup Rim ),송원섭 ( Won-seob Song ),서영미 ( Young-mi Seo ),박승택 ( Seung-taeck Park ),김신무 ( Shin-moo Kim ) 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.3

This study was aimed to clerify the cytotoxicity of some plant extracts such as Hosta longissima HONDA (HL), Hemerocallis fulva var. Kwanso REGL (HFVK), Hemerocallis fulva L (HF), Macrocapium officinale NAKAI (MO) and Mentha canadensis var. piperascens HARA (MCVP), the cultured NIH3T3 fibroblasts were treated with 25, 50, 100, 150 and 200 μg/mL of five kinds of plant extracts for 48 hours, respectively. The cytotoxicity of plant extracts was measured by MTT and NR assays for the cell viability, and XTT assay for the cell adhesion activity. In this study, HL, MO and FHVK extracts showed the range of midtoxic-non toxic by the criteria of chemical cytotoxicity. While, the HF and MCVP extracts showed midtoxic. In the extract cytotoxicity, HL, MO and FHVK extracts showed non-toxic by the criteria of extract cytotoxicity. While, HF extract was determined as lower-toxic. In the responsive sensitivity of each plant extract on colorimetric assays, HF extract was sensitive to mitochondrial enzyme by MTT assay, lysosomal enzyme by NR assay and mitochondrial nucleus by XTT assay. While, MCVP extract was sensitive to mitochondrial enzyme by MTT assay and lysosomal enzyme by NR assay than other assays. While, HL, HFVK and MO extracts were most sensitive to NR assay. Cell culture is one of useful materials in the screening of cytotoxic and recovary effect on the putative chemical agents or plant extract. And also, colorimetric assay is regarded as very useful tools for quantitative measurement of cytotoxic effect on plant extracts in vitro.

Paraquat의 세포독성과 흰쥐의 폐에서 3-Methylcholanthrene의 독성경감효과

임요섭(Yo-Sup Rim),김덕수(Doc-Soo Kim),한두석(Du-Seok Han),황인택(In-Taek Hwang) 한국농약과학회 2002 농약과학회지 Vol.6 No.2

제초제 paraquat와 bentazon의 세포독성과 3-methylcholanthrene의 독성경감효과

임요섭(Yo Sup Rim),서대호(Dae Ho Seo),한두석(Du Seok Han) 한국독성학회 2001 Toxicological Research Vol.17 No.2

This study were carried out to investigate cytotoxicity oj paraquat and bentazon that is scattering to farm products were essential for human diet and compensatory effects of 3-methylcholanthrene (3-MC) in vitro and in vivo. In vitro, The 5.0×10⁴cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution of paraquat and bentazon (1, 25, 50, 100 J.1M respectively). After the NIH 3T3 Jib rob last of all groups were cultured in same condition for 48 hours, Sulfohordamin B Protein (SRB) assay were performed to evaluate the cytotoxicity of cell organelles. Paraquat and bentazon SRB50 were 1860.73 J.μM, 1913.38 J.μM respectively. In vivo, Sprague Dawley male rats divided into paraquat and bentazon only administered group and simultaneous application group of paraquat and bentazon and 3-MC. At 30 min. and I, 3, 6, 12,24,48 and 96 hrs. interval after each treatment, the animals were sacrificed by decapitation and kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM, and PAS. Under the light microscope, atrophic change of renal corpuscles were frequently observed from 3 hrs after paraquat and bentazon treatment. The increase of the mesangium was apparent 12 hrs later after paraquat and bentazon treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 48 hrs after paraquat and bentazon treatment, respectively. In contrast there were no evidences of the toxic effects on renal tissues at 48hrs in paraquat and bentazon plus 3-MC treated groups.

살충제 Carbofuran과 Phenobarbital Sodium 및 3-Methylcholanthrene이 쥐의 효소활성에 미치는 영향

임요섭(Yo-Sup Rim),한성수(Seong-Soo Han) 한국농약과학회 1999 농약과학회지 Vol.3 No.3

The Effects of Ethephon Application on Suppressing Methane Emission and Stimulating Rice Productivity in a Rice Paddy Soil: A Pot Experiment

Seongwoo Choi,Juhee Lee,Yeomyeong Lee,Pil Joo Kim,Ju-Sik Cho,Yong Hwa Cheong,Yo-Sup Rim,Sang Yoon Kim 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.4

Ethephon is one of the chemical stimulator of crop growth that is also considered as a structural analogue of coenzyme M (CoM), having a promising potential for methanogenic inhibitor. However, the effect of ethephon application on greenhouse gas (GHG) emissions under rice cultivation has not been studied yet. In this pot experiment, different levels of ethephon (0, 2.5, 5 and 10 ㎎ L<SUP>-1</SUP>) were applied at the presence or absence of manure compost as an organic amendment to study its effect on GHGs emissions in particular CH₄ and rice productivity during the cultivation. Application of ethephon effectively suppressed CH₄ emissions in particular the initial periods (ca. 3 weeks) right after the application as compared with control soil during rice cultivation, but did not significantly affect N₂O emissions. In addition, the maximum CH₄ reduction (82% reduction over control) was found at 10 ㎎ L<SUP>-1</SUP> ethephon application right after its addition for 3 weeks during rice cultivation. Rice productivity and yield properties were mainly affected by ethephon application, enhancing ripened grains and number of grains per panicle. Soil properties were not influenced by ethephon application except for available phosphate, which is probably due to enhanced root growth in rhizosphere soils. Conclusively, ethephon could be a new and pioneering amendment for reducing CH₄ emission in particular at the initial 3 weeks after the application without increasing other GHG emissions and enhancing rice productivity in paddy soils.

웹 기반의 다이렉트 볼륨 렌더링 View 프로그램의 설계 및 구현

윤요섭(Yoon Yo-Sup),윤가림(Yoon Ga-Rim),김영봉(Kim Young-Bong) 한국콘텐츠학회 2004 한국콘텐츠학회 종합학술대회 논문집 Vol.2 No.2

인터넷은 사용이 매우 간편한 도구인 윌드와이드웹의 등장으로 인하여 가장 독보적이면서도 강력한 지원을 제공하는 세계에서 가장 간편한 네트워크 자원이 되었다. 더 나아가 정적인 2차원을 넘어서 3차원 View 웹 서비스와 같은 동적인 서비스를 제공하는 많은 방법들이 제시되었다. 본 논문계서는 MRI, CT, PET 같은 2차원 의료 영상을 쌓아 만든 3차원 데이터를 웹에서 인터액티브하게 가시화 하는 볼륨 렌더링 View 프로그램을 제시할 것이다. 이를 위해 우리는 COM기술을 바탕으로 하는 ActiveX 컴포넌트인 OCX 컨트롤로 만들어 웹 페이지에서 살현 가능하도록 하였다. 또한 인터넷만 있으면 쉽게 원격에서도 3차원 가시화를 통한 영상 분석을 할 수 있는 기능을 제공하여 질병의 진단에 크게 기여 할 것으로 기대된다. Since the world wide web, simple and convenient tool, has proposed, the Internet became the most simple network resource which provide many informations of the world. Furthermore, various methodologies are developed to support the dynamic service such as 3D View web service. We will propose the volume rendering view program that interactively visualize the 3D data on the web. The 3D Data is obtained by stacking the 2D images along the z-direction. We also employ the COM based OCX control which is a kind of Active component. This web program will contribute the diagnosis of the diseases through the 3D visualization and image analysis functions at remote places.

목 부분의 제거를 통한 얼굴 검출 향상 기법

윤가림(Yoon Ga-Rim),윤요섭(Yoon Yo-Sup),김영봉(Kim Young-Bong) 한국콘텐츠학회 2004 한국콘텐츠학회 종합학술대회 논문집 Vol.2 No.2

보통 사람의 정면과 측면 사진 등을 이용하여 3차원 얼굴 모델에 텍스처를 입히는 많은 연구들이 수행되어 오고 있다. 많은 좋은 결과들이 나타나고 있다. 정면 사진이나, 측면 사진에서 사람의 눈, 코 입 등의 위치를 정확히 추출하는 일은 이 작업에서 가장 중요한 요소라 할 것이다. 기존의 연구들은 눈의 위치 혹은 입이나 코의 위치를 대충 찾은 다음에 그 상관관계에 따라 얼굴의 각 요소를 추출해 내는 방법을 많이 쓰고 있다. 이 때 가장 문제가 되는 부분이 목 부분이 제대로 인식되어 얼굴 부분에서만 얼굴 요소를 찾으면 정확한 결과를 보이는데, 목과 턱 선의 구분의 불명확으로 나타난 경우에는 잘못된 결과를 보이고 있다. 따라서 본 연구에서는 전체의 얼굴 영상에서 턱 아래 부분을 제거함으로써 얼굴 요소의 검출이 향상되도록 하는 알고리즘을 제시할 것이다. 턱 아래 부분의 완전한 제거를 위해 본 연구에서는 RGB값과 그 기울기를 이용하여 목 부분을 효율적으로 제거하는 방법을 제시할 것이다. Many researchers have been studied texturing the 3D face model with front and side pictures of ordinary person. It is very important to exactly detect the psition of eyes, nose, mouth of a human from the side pictures. Previous results first found the position of eye, nose, or mouth and then extract the other face components using their positional correlation. The detection results greatly depend on the correct extraction of the neck from the images. Therefore, we present a new algorithm that remove the neck completely and thus improve the detection rates of face components. To do this, we will use the RGB values and its differences.

The Cytotoxic Effects Paraquat and Bentazon Compensatory Effects of 3-methylcholanthrene on Kindney of the Rat

Doc Soo Kim,Yo Sup Rim,Du Seok Han,Seong Soo Han 한국토양비료학회 2001 한국토양비료학회 학술발표회 초록집 Vol.2001 No.5

우분뇨와 왕겨 혼합물의 정치식과 통기퇴적식 퇴비화 과정에서 CO2 및 NH3 가스 발생과 토마토 생육

홍지형,임요섭,양원모,김길용,손보균,박금주 한국환경농학회 1997 한국환경농학회지 Vol.16 No.2

비닐온실내의 간헐 통기방식의 우분뇨 퇴비화에서 발효열에 의한 근권토양의 지온변화와 CO₂ 및 부숙최기의 NH 가스발생양상을 조사하고 토마토 재배를 통하여 그 혜택을 구명하고자 수행하였다. 퇴비화시설 온실 30㎝깊이의 지중온도 변화는 퇴비재료의 부숙온도 상승에 따라 지중온도 증가를 보여 부숙개시후 1주일에 최고 32℃까지 상승하여 대조구에 비해 18℃의 차이를 보였고, 부숙개시 20일 후부터는 주발효의 종료와 함께 점차 온도감소를 나타냈으며 35일 이후는 23℃수준을 일정하게 유지하는 반면에 대조구인 관행온실의 지중온도는 70일동안 14∼15℃범위를 벗어나지 않아 퇴비발효열에 의한 토마토 근권토양의 가온효과가 확인되었다. 전 조사기간에 퇴비화 온실에서 발생되는 CO₂ 평균값은 782∼1154ppm으로 관행온실(대조구) 440∼462ppm수준에 비해서 1.7∼2.6배의 차이를 보였다. 간헐통기 퇴비화에서 발생하는 NH₃ 가스는 부숙개시 후 3∼10일내에 다량 방출되었으며, 5일째 조사시 최고치인 134ppm을 보인 후 감소하여 17일부터는 3∼4ppm수준을 나타냈다. 비닐하우스내 밀폐 강제통기 퇴비화방식은 퇴비재료가 부숙되는 동안 방출되는 CO₂와 발효열에 의한 광합성 증대와 작물근권 온도를 높여 주는 효과로 인해 토마토의 생육, 수량 및 당도의 증가를 보였으며 총과중의 경우 60%의 증수를 나타냈다. This study was performed to evaluate the influence of composting process with an intermittent aeration on the variation of rhizosphere soil temperature, CO₂ and NH₃ release, and the growth reponse of tomato plantlet in traditional and composting greenhouse. As the temperature of composting materials increased, rhizosphere soil temperature in 30㎝ depth rose up to 32℃ at one week after introduction. This was 18℃ higher than that of traditional greenhouse. After 20 days of active composting, temperature of rhizosphere soil started to decrease and remained constant at 23℃ after 35 days. For the traditional greenhouse, the averaged temperature ranged at 14∼15℃. This results showed that composting greenhouse had the greater effect on increasing the underground temperature. Average value of evoluted CO₂ from the composting greenhouse for 70 days was 782∼1154ppm. This was 1.7∼2.6 times higher than that of the traditional greenhouse with an average of 440∼462 ppm. NH₃ release was highest during 2∼10 days in intermittent aerated composting and reached to 134 ppm maximum on the 5th day, then decreased rapidly, and maintained at 3∼4 ppm after 17 days. Increased photosynthesis due to the CO₂ gas and a favorable rhizosphere environment due to the increased underground temperature resulted in improved growth, yield, and Brix degree of tomato fruit.