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Ying Shan,Ying Dong,Dong Jiang 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6
Antibiotic peptides are a battery of broadspectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human a-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel- Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.
Novel Architecture Design of Large-Scale Distributed Object Storage System
Shan Ying,YAO Nian-min 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1
A novel architecture design of large-scale distributed object storage system (called DOSS) is proposed. Our design takes several aspects effecting on the overall performance of DOSS into consideration, including an improved model of interaction based on the traditional interactive mode of object-based storage systems, MDS(Metadata Server) management scheme and a load balancing scheme combines the definition of the maximum load for OSD(Object Storage Device), hierarchical model and re-polling in order to solve access of heat objects issues. Our experimental results show that the architecture is an effective way to promote the performance of DOSS.
A Cache Design of Load Balancing System for Object-Based Storage
Shan Ying,Liu Dan,Nian-min Yao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.6
In design of the object-based storage system, cache design of the server becomes an important guarantee for improving service quality. This paper presents a cache design of load balancing system for object-based storage, which consists of three main aspects. First, the cache update policy is proposed. The strategy considers the overall cache as a pool, consistency and accuracy of updating is ensured through the locking mechanism, while using Bloom Filter combined with the updated time series to achieve updating simplification. Secondly, the cache replacement strategy is introduced which achieving the cache replacement policy using cache spanning tree combined with cache stack. Finally, cache design model is proposed based on energy consumption. Experimental results show that the proposed cache design can improve the efficiency of cache operation in object-based storage system, while reducing energy consumption of cache operation.
Shan, Ying,Dong, Ying,Jiang, Dong 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6
Antibiotic peptides are a battery of broad-spectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human a-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel-Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.
Nonlinear Adaptive Decentralized Stabilization Control for Multimachine Power Systems
Shan-Ying Li,이상성,윤용태,박종근 제어·로봇·시스템학회 2009 International Journal of Control, Automation, and Vol.7 No.3
This paper presents a decentralized adaptive backstepping controller to dampen oscillations and improve the transient stability to parametric uncertainties in multimachine power systems. The proposed design on the ith synchronous generator uses only local information and operates without the need for remote signals from the other generators. The design of the nonlinear controller is based on a modified fourth-order nonlinear model of a synchronous generator, and the automatic voltage regulator model is considered so as to decrease the steady state voltage error. The construction of both the con-trol law and the associated Lyapunov function is systematically designed within the design methodol-ogy. A 3-machine power system is used to demonstrate the effectiveness of the proposed controller over two other controllers, namely a conventional damping controller (power system stabilizer) and one designed using the feedback linearization techniques.
Topological Analysis of Chaos Characteristics in a Power System
Li, Shan-Ying,Lee, Sang-Seung,Park, Jong-Keun The Korean Institute of Electrical Engineers 2004 KIEE International Transactions on Power Engineeri Vol.a4 No.1
This paper proposes a totally new method in the chaos characteristics' analysis of power systems, the introduction of topological invariants. Using a return histogram, a bifurcation graph was drawn. As well, the periodic orbits and topological invariants - the local crossing number, relative rotation rates, and linking number during the process of period-doubling bifurcation and chaos were extracted. This study also examined the effect on the topological invariants when the sensitive parameters were varied. In addition, the topological invariants of a three-dimensional embedding of a strange attractor were extracted and the result was compared with those obtained from differential equations. This could be a new approach to state detection and fault diagnosis in dynamical systems.