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( Yijing Wu ),( Chao Zhao ),( Zheng Xiao ),( Hetong Lin ),( Lingwei Ruan ),( Bin Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12
A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Twodimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple “omics” analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.
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Cooperative spectrum leasing using parallel communication of secondary users
( Ping Xie ),( Lihua Li ),( Junlong Zhu ),( Jin Jin ),( Yijing Liu ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.8
In this paper, a multi-hop transmission protocol based on parallel communication of secondary users (SUs) is proposed. The primary multi-hop network coexists with a set of SUs by cooperative spectrum sharing. The main optimization target of our protocol is the overall performance of the secondary system with the guarantee of the primary outage performance. The energy consumption of the primary system is reduced by the cooperation of SUs. The aim of the primary source is to communicate with the primary destination via a number of primary relays. SUs may serve as extra decode-and-forward relays for the primary network. When an SU acts as a relay for a primary user (PU), some other SUs that satisfy the condition for parallel communication are selected to simultaneously access the primary spectrum for secondary transmissions. For the proposed protocol, two opportunistic routing strategies are proposed, and a search algorithm to select the SUs for parallel communication is described. The throughput of the SUs and the PU is illustrated. Numerical results demonstrate that the average throughput of the SUs is greatly improved, and the end-to-end throughput of the PU is slightly increased in the proposed protocol when there are more than seven SUs.
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