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        A new species of Hedysarum (Fabaceae, Hedysareae) from Xizang (Tibet), China.

        최병희,Yasuhiko Endo,Xiang-yun Zhu 한국식물분류학회 2011 식물 분류학회지 Vol.41 No.3

        A new species of Hedysarum (Fabaceae, Hedysareae) was found in Tibet, China. This new species, Hedysarum hirtifoliolum, belongs to sect. Hedysarum and is readily distinguishable in having greenish yellow flowers, pubescent above surface of leaflets and transversely obovate loments. So far, it is collected from only one locality in Tibet.

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        Improvement of Ti-Plasma Coating on Ni-Ti Shape Memory Alloy Applying to Implant Materials and its Evaluation

        Okuyama, Masaru,Endo, Jun,Take, Seisho,Itoi, Yasuhiko,Kambe, Satoshi 한국부식방식학회 2002 Corrosion Science and Technology Vol.31 No.6

        Utilizing of Ni-Ti shape memory alloy for implant materials has been world-widely studied. It is, however, known that Ni-Ti alloy is easily attacked by chloride ion contained in body liquid. To prevent Ni dissolution, the authors tried to coat the alloy surface with titanium metal by means of plasma-spray coating method. The plasma coating films resulted in rather accelerating pitting corrosion because of their high porosity. Therefore, sealing of the porous films was required. In order to solve this problem and satisfy prolonged lifetime in the body, the authors tried to use the vacuum evaporation technique of titanium metal. Two types of Ti vacuum evaporation procedures were employed. The one was to cover a thin film on Ni-Ti alloy surface prior to massive Ti plasma spray coating. The other was to first coat plasma spray films on Ni-Ti alloy and then to cover them with vacuum evaporation films of Ti. Protective ability against pitting corrosion was examined by electrochemical polarization measurement in physiological solution and the coating films were characterized by microscopic and SEM observation and EPMA analysis. Vacuum evaporation thin films could not protect Ni-Ti alloy from pitting corrosion. In the case of plasma spray coating over the Ti vacuum evaporation thin film, the substrate Ni-Ti alloy could not be better protected. On the contrary, vacuum evaporation of Ti over the porous plasma spray coating layer remarkably improved corrosion protective performance.

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        Basic Verification of β-D Glucan in Leukocyte-Rich Plasma for the Diagnosis of Deep Mycosis

        Shimoyama Ken,Kan Shigenori,Takahashi Gaku,Morino Gota,Yamada Yasuhiko,Inoue Yoshihiro,Inada Katsuya,Endo Shigeatsu 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.1

        Background: Currently, supplementary serological testing for β-D glucan (BDG) is often selected to diagnose deep mycosis in care covered by the health insurance in Japan. The Wako method used by our center has low sensitivity, and different studies have used different cut-off values due to factors that cause false positives and false negatives. One possible cause of false negatives is the use of platelet-rich plasma (PRP) as the sample material. Because phagocytic white blood cells (WBC) are precipitated by centrifugation and only plasma is measured, it seems unlikely that the actual amount of BDG is being measured when using PRP. Further, a frequent cause of false positives is contamination from blood products and gauze containing BDG. To resolve these issues, the blood cell separator, hydroxyethyl starch, is used to precipitate only the red blood cells to obtain leukocyte-rich plasma (LRP). We hypothesized that it might be possible to improve the diagnostic rate of deep mycosis by measuring the BDG content of plasma containing WBC and fungal components and by comparing the BDG content of PRP and LRP measured simultaneously. Materials and Methods: Healthy human blood, albumin-added blood, wrung-out gauze fluid-added blood, and fungal solution-added blood were prepared, and PRP and LRP were prepared using hydroxyethyl starch. The BDG content of each sample was measured using the Wako method and compared. In addition, PRP and LRP of fungal-added blood were Gramstained and examined under a microscope, and the number of WBCs and phagocytosed fungi was counted visually and compared. Results: Measuring the BDG content of LRP confirmed that there were no false positives with LRP, and in vitro experiments comparing albumin-added false-positive blood to fungal-added blood showed significant differences between PRP and LRP only in the fungal-added blood. Conclusion: Calculating the BDG-ratio (LRP/PRP) by measuring both LRP and PRP may eliminate false positives and false negatives of true deep mycosis and improve the diagnostic rate.

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