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Xiong, Wei,Jiang, Yong-Xin,Ai, Yi-Qin,Liu, Shan,Wu, Xing-Rao,Cui, Jian-Guo,Qin, Ji-Yong,Liu, Yan,Xia, Yao-Xiong,Ju, Yun-He,He, Wen-Jie,Wang, Yong,Li, Yun-Fen,Hou, Yu,Wang, Li,Li, Wen-Hui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Background: Preoperative 5-fluorouracil (5-FU)-based chemoradiotherapy is a standard treatment for locally advanced colorectal cancer (CRC). However, CRC cells often develop chemoradiation resistance (CRR). Recent studies have shown that long non-coding RNA (lncRNA) plays critical roles in a myriad of biological processes and human diseases, as well as chemotherapy resistance. Since the roles of lncRNAs in 5-FU-based CRR in human CRC cells remain unknown, they were investigated in this study. Materials and Methods: A 5-FU-based concurrent CRR cell model was established using human CRC cell line HCT116. Microarray expression profiling of lncRNAs and mRNAs was undertaken in parental HCT116 and 5-FU-based CRR cell lines. Results: In total, 2,662 differentially expressed lncRNAs and 2,398 mRNAs were identified in 5-FU-based CRR HCT116 cells when compared with those in parental HCT116. Moreover, 6 lncRNAs and 6 mRNAs found to be differentially expressed were validated by quantitative real time PCR (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the differentially expressed mRNAs indicated involvement of many, such as Jak-STAT, PI3K-Akt and NF-kappa B signaling pathways. To better understand the molecular basis of 5-FU-based CRR in CRC cells, correlated expression networks were constructed based on 8 intergenic lncRNAs and their nearby coding genes. Conclusions: Changes in lncRNA expression are involved in 5-FU-based CRR in CRC cells. These findings may provide novel insight for the prognosis and prediction of response to therapy in CRC patients.
Dependence of viscoelastic parameters of nematic liquid crystals on pretilt angle and temperature
Yao-Xiong Huang,Mei Tu 한국물리학회 2010 Current Applied Physics Vol.10 No.2
The viscoelastic parameters of nematic liquid crystal (LC) E7 in both splay and twist relaxation modes are investigated as functions of pretilt angle and temperature by the technique of dynamic laser light scattering. The results show that the elastic constants of the liquid crystal in the two modes not only depend on temperature, but also depend on pretilt angle. There is a critical pretilt angle (βC) at which nematic LC begins exhibiting elastic property, and beyond which the elastic constants increase with pretilt angle,then keep constant after exceeding another angle (βS, with βS > βC). This phenomenon is observed to be universal for different nematic LC’s, and significant not only in the understanding of the molecular mechanism of exhibiting viscoelastic properties in nematic LC, but also for their practical purpose.
Yao-Xiong Huang 한국물리학회 2005 Current Applied Physics Vol.5 No.5
A novel microscope light scattering spectroscopy and image analyzing system has been developed for simultaneous measure-ments on particle size distribution in solutions with size ranging from nanometer to sub-millimeter, and for surface porosity analysison solid materials. The present paper introduces the technique of this system and its applications to various materials..
Membrane surface charge and morphological and mechanical properties of young and old erythrocytes
Xing-Yao Chen,Yao-Xiong Huang,Wen-jing Liu,Zhi-jian Yuan 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
ditions were studied as a function of cell age. The young and old cells were separated from fresh rabbit blood by using Percoll densitygradients. A novel multi-dimensional microscope was employed to perform real-time, non-invasivein situmeasurements on the mem-brane bending elastic modulus and the cell shape and size. A phase-analysis micro-electrophoresis laser scattering technique was usedto measure the surface charge density. The results show that the membrane Zeta potential of red blood cells is reduced when they become.
Xiong, Ai Sheng,Yao, Quan-Hong,Peng, Ri-He,Li, Xian,Fan, Hui-Qin,Guo, Mei-Jin,Zhang, Si-Liang Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.3
Phytases catalyze the release of phosphate from phytic acid. Phytase-producing microorganisms were selected by culturing the soil extracts on agar plates containing phytic acid. Two hundred colonies that exhibited potential phytase activity were selected for further study. The colony showing the highest phytase activity was identified as Aspergillus niger and designated strain 113. The phytase gene from A. niger 113 (phyI1) was isolated, cloned, and characterized. The nucleotide and deduced amino acid sequence identity between phyI1 and phyA from NRRL3135 were 90% and 98%, respectively. The identity between phyI1 and phyA from SK-57 was 89% and 96%. A synthetic phytase gene, phyI1s, was synthesized by successive PCR and transformed into the yeast expression vector carrying a signal peptide that was designed and synthesized using P. pastoris biased codon. For the phytase expression and secretion, the construct was integrated into the genome of P. pastoris by homologous recombination. Over-expressing strains were selected and fermented. It was discovered that ~4.2 g phytase could be purified from one liter of culture fluid. The activity of the resulting phytase was 9.5 U/mg. Due to the heavy glycosylation, the expressed phytase varied in size (120, 95, 85, and 64 kDa), but could be deglycosylated to a homogeneous 64 kDa species. An enzymatic kinetics analysis showed that the phytase had two pH optima (pH 2.0 and pH 5.0) and an optimum temperature of $60^{\circ}C$.
Few-shot transfer learning with attention for intelligent fault diagnosis of bearing
Yao Hu,Qingyu Xiong,Qiwu Zhu,Zhengyi Yang,Zhiyuan Zhang,Dan Wu,Zihui Wu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12
The bearing is one of the key components in modern industrial equipment. In the past few years, many studies have been carried out on bearing diagnosis through datadriven methods. However, there are two practical problems. First, under actual working conditions, the lack of fault samples is a major factor that hinders the application of these methods in industrial environments. Second, there is a lack of full utilization of a priori knowledge in the current stage of methods using relational networks for fault diagnosis. It is manifested by the incompleteness of the relational network structure. To address these problems, we present a new diagnosis method based on few-shot learning, which is suitable for the environment where the data is scarce. In this method, we train the model with the data generated by the artificial damaged bearings instead of the data from the real bearing. We experimentally validate the performance improvement of the complete relational network structure. It is able to perform the few-shot learning task better. In addition, we also reduce the global feature discrepancy by introducing an attention mechanism to improve the performance of the model. And the impact of the number of layers of the attention mechanism on the model is also discussed in detail. In this paper, our model performs better under the same experimental conditions compared with other transfer learning models.
Directed Evolution of Beta-galactosidase from Escherichia coli into Beta-glucuronidase
Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Xu, Fang,Cai, Bin,Guo, Zhao-Kui,Qiao, Yu-Shan,Chen, Jian-Min,Zhang, Zhen,Yao, Quan-Hong Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3
In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.
Jia, Yao,Hu, Ting,Hang, Chuan-Ying,Yang, Ru,Li, Xiong,Chen, Zhi-Lan,Mei, Ye-Dong,Zhang, Qing-Hua,Huang, Ke-Cheng,Xiang, Qun-Ying,Pan, Xiu-Yu,Yan, Yu-Ting,Wang, Xiao-Li,Wang, Shao-Shuai,Hang, Zhou,Tang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10
Purpose: To investigate the diet of patients with cervical cancer and precancerosis in the Wufeng area, a high-incidence region in China. Methods: In the case group, 104 patients diagnosed with cervical cancer or cervical intraepithelial neoplasias (CINII/III) were recruited from the Wufeng area. Nine hundred thirty-six healthy women were selected from the same area as the matched controls. A questionnaire, which included questions about general lifestyle conditions, smoking and alcohol status, source of drinking water, green tea intake, and diet in the past year, was presented to all participants. Results: Green tea intake (P=0.022, OR=0.551, 95% CI=0.330-0.919) and vegetable intake (P=0.035, OR=0.896, 95% CI=0.809-0.993) were identified as protective factors against cervical cancer or CINII/III. There was no indication of any associations of other lifestyle factors (smoking status, alcohol status, source of drinking water) or diet (intake of fruit, meat/egg/milk, soybean food, onion/garlic, staple food and pickled food) with cervical cancer. Conclusions: The results suggest that eating more fresh vegetables and drinking more green tea may help to reduce the risk of cervical cancer or CINII/III in people of the Wufeng area.
Yuelan Yao,Hanjie Ying,Jian Xiong,Yong Chen,Jiapeng Tang 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.1
The process of ATP biosynthesis from adenosine catalyzed by Saccharomyces cerevisiae was studied using an efficient energy regeneration system. A fractional factorial design (2^(9-5)) was used to evaluate the effects of different components in the medium. Magnesium chloride, toluene, and acetaldehyde were found to significantly influence ATP production. The concentrations of the three factors were then optimized using central composition design and response surface analysis. Based on the second-order polynomial model obtained from the experiments, the optimal parameters were obtained as follows: adenosine 20 g/L; glucose 67 g/L; S. cerevisiae cells 250 g/L; magnesium chloride 4.37 g/L;potassium dihydrogen phosphate 67 g/L; toluene 1.40 mL/L; acetaldehyde 2.67 mL/L; pH 7.0; and temperature 37.0 ℃. Under the condition, the yield and concentration of ATP reached 97.5% and 37 g/L, respectively. The yield was nearly 10% higher than the level before optimization and the concentration increased two-fold. In addition, the utilization efficiency of energy after optimization increased nearly 6%.
Pan, Xiong-Fei,Yang, Shu-Juan,Loh, Marie,Xie, Yao,Wen, Yuan-Yuan,Tian, Zhi,Huang, He,Lan, Hui,Chen, Feng,Soong, Richie,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4
Objectives: Interleukin (IL) -10 is a potent cytokine with a dual ability to immunosuppress or immunostimulate. We aimed to explore the association of IL10 promoter polymorphisms with risk of gastric cancer (GC) in a Han population in Southwestern China. Methods: We enrolled 308 pairs of GC and control subjects from four hospitals and a community between October 2010 and August 2011 in a 1:1 matched case-control design. Demographic information was collected using a designed questionnaire. IL10-592 A>C and IL10-1082 A>G polymorphisms were determined by Sequenom MassARRAY analysis. Results: Patients with GC reported statistically higher proportions of family history of cancer (29.9% versus 10.7%, P<0.01) and alcohol drinking (54.6% versus 43.2%, P<0.01) than did controls. Similar results were observed in comparison between non-cardia GC patients and controls (P<0.01 and P=0.03). Variant genotypes of IL10-592 A>C and IL10-1082 A>G were not associated with overall GC risk (adjusted OR, 0.94, 95% CI, 0.66-1.33; adjusted OR, 1.00, 95% CI, 0.62-1.60). Sub-analysis showed that the IL10-592 AC/CC variant genotype was associated with decreased non-cardia GC risk (adjusted OR, 0.58; 95% CI, 0.36-0.95). No association was found between any of the IL10 haplotypes established from two polymorphisms and risk of non-cardia GC. Conclusions: In conclusion, our data do not link the two SNPs of IL10-592 and IL10-1082 with overall GC risk. We demonstrate that IL10-592 polymorphism is associated with protective effect against non-cardia GC. Our findings may offer insight into risk associated with the development of GC in this region.