Male Sterile (MS) line to pollen parent ratio of oilseed rape on fatty acid compositions and yield performances

Yang-Gyu Ku,Sun-Young Yang,Yong-Su Jung,Jang-Ock Kim,Young-Seok Jang,Mi-Chung Suh,Sung-Ju Ahn 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Brassica napus L. (Brassicaceae) plantation has increased as its seed is the primary source for bio-diesels. This study was conducted Male sterile (MS) line (cv. 'Sun-Mang') to pollen parent (cv. 'Mokpo 64') ratio of rape plants on fatty acid compositions of six sequential stages and yield performances. Ratio of MS line to pollen parent was followed, 4:2, 10:1, and 10:2, respectively. Seeds of six sequential stages (35, 40, 45, 50, 55, and 60 days after flowering) were collected and determined using SEED FAME ESTIMATION. The results showed that oleic acid content (C18:1) of the MS to pollen parent ratio (4:2) relatively increased up to 60 days after flowering compared to other ratios. Other fatty acid compositions such as palmitic acid (C16:0), stearic acid (C18:0), linoleic acid (C18:2) and linolenic acid (C18:3) contents decreased during seed maturation period. Seed weight (1000 seeds) of MS to pollen parent ratio (4:2) was higher than other treatments. These results suggest that fatty acid compositions and seed weight was affected by ratio of MS line to pollen parent. For rape seed production, a suitable ratio of MS line to pollen parent will be required to cultivate rape plantations.

담즙산으로 유도된 백서의 췌장손상에 대한 Prostaglandin E1의 효과

서정일,양진석,김학산,남홍우,이봉휘,송형운,지영구,이태광,한창순,한길로 대한소화기학회 1991 대한소화기학회지 Vol.23 No.2

To elucidate the cytoprotective effect of prostaglandin on pancreatic injury, we measured the mortality rate, the degree of pancreatic necrosis, and serum amylase level after prostaglandin E₂ administration in acute experimental pancreatitis which was induced by the retrograde injection of 5% sodium taurocholate into the biliopancreatic duct of rat. Prostaglandin E₁ (100 ㎍/㎏ Body Weight) were injected subcutaneously after 1 and 6 hour later after taurocholate injection, and normal saline solution (1 ㎖) to the control group by same method. The obstained results were as follows: 1) The mortality rate upto 72 hour was not different (11%: VS 29 %) in both group, but mortality rate upto 96 hour was significantly reduced in prostaglandin group compared with that of control group (11% vs 58% p$lt;0.05). 2) The mean serum level of anylase of control group was 773±55.2U and that of prostaglanin group was 772±61.1 U, which were not significantly different each other. 3) The degree of pancreatic necrosis was also reduced in prostaglandin group compared with control group. In conclusion, the present study suggest that prostaglandin E₂ will have a benefical effect on the course of acute pancreatic injury induced by sodium taurocholate.

Physiological Response, Fatty Acid Composition and Yield Component of Brassica napus L. under Short-term Waterlogging

Yang-Gyu Ku(구양규),Won Park(박원),Jin-Ki Bang(방진기),Young-Seok Jang(장영석),Yong-Beom Kim(김용범),Hyun-Jong Bae(배현종),Mi-Chung Suh(서미정),Sung-Ju Ahn(안성주) (사)한국생물환경조절학회 2009 시설원예‧식물공장 Vol.18 No.2

단기간 침수처리에 의한 유채의 영양쟁장과 생식생장기의 생리적 반응, 지방산 조성과 수확량에 관하여 조사하였다. 영양생장 단계에서 10일과 생식생장 단계에서 4일 침수한 결과를 영양생장과 생식생장기 모두 광합성, 기공전도도와 증산량은 침수처리 기간과 회복시기에 현저하게 감소하였다. 침수처리가 끝난 처리구에서 광합성과 기공전도도는 2~3일 내에 대조구와 비슷한 경향을 보였다. 침수처리된 작물의 지방산 조성은 대조구에 비해 통계적 유의성의 차이를 보이지 않았지만 수확량과 관련된 요인들에서 현저하게 감소함으로 생식생장 단계에서의 단기간 침수처리가 수확량 요인에 큰 영향을 줌으로서 종지 수확량에 영향을 미칠 것으로 사료된다. The effects of short-term waterlogging on physiological responses, fatty acid compositions and yield components of rapeseed at both the vegetative growth and the reproductive growth stages were assessed in this study. Waterlogged plants were treated for a period of 10 days at the vegetative growth stage and 4 days at the reproductive growth stage. The results show that photosynthesis and stomatal conductance at both the vegetative growth and the reproductive growth stage were significantly reduced during the waterlogging period and after the recovery period. When the plants were removed from water, photosynthesis and stomatal conductance progressively restored similar values to those of control plants within 2~3 days. Fatty acid compositions were unaffected by waterlogging treatment. However, yield components (pod number and pod length) of the waterlogged treated plants at the reproductive growth stage were significantly reduced. These results suggest that short-term waterlogging may thus influence oilseed yield component.

Photosynthetic activity, fatty acid composition and yield performances of rapeseed plants to drought and waterlogging

Yang-Gyu Ku,Won Park,Jin-Ki Bang,Young-Seok Jang,Mi-Chung Suh,Sung-Ju Ahn 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Brassica napus L. (Brassicaceae), is one of major oilseed crops commonly cultivated cereal-growing areas after the rice harvest in Asia countries, has recently focused much attention as its seed is the primary source for bio-diesels. In rapeseed growing regions of South Korea, typical double-cropping regions are constantly or periodically received with poorly drained soil condition or encountered dried soil condition during the reproductive stage. This study was conducted on rapeseed plants subjected to both waterlogging and drought conditions for 4 days to investigate responses in physiological characteristics, fatty acid compositions and yield performances at reproductive stage. The treatments were thus as follows: (1) control normally irrigated, (2) waterlogging treatment were flooded by placing their pots inside larger plastic pots filled with tap water with a 2-cm water layer over soil surface, (3) drought treatment was not irrigated. For recovery period, rapeseed plants subjected to waterlogging for 4 days were drained after waterlogging, and irrigated daily as control. Drought treatment after recovery period was irrigated daily as control. Rate of photosynthesis and stomatal conductance of waterlogging and drought treated plants significantly decreased within 4 days. For recovery period, stomatal conductance and photosynthetic rate of the waterlogged and drought treated plants occurred level similar to that of the controls by 2 days after recovery period. Fatty acid compositions of the both waterlogging and drought treated plants did not affect, but yield performances of the waterlogging treatment significantly decreased. These results suggest that both waterlogging and drought conditions for 4 days did not influence fatty acid compositions, but affected yield performance at final harvest.

Genetic Diversity and Differentiation in Remnant Populations of Bupleurum latissimum Nakai, an Endangered Endemic Plant Species to Ulleung Island, Korea

Ku, Youn-Bong,Oh, Hyun-Kyung,Kong, Hak-Yang,Suh, Min-Hwan,Lee, Min-Hyo,Sviatlana, Trybush,Cho, Kang-Hyun The Korean Society for Integrative Biology 2004 Korean journal of biological sciences Vol.8 No.4

Bupleurum latissimum is a narrowly endemic and endangered plant, restricted to only two small populations on steep cliffs of a small island, Ulleung Island, in Korea. The genetic diversity and population differentiation in the two remnant populations of the species were investigated using RAPD (random amplified polymorphic DNA) analysis. The Neis gene diversities were 0.146 in the smaller population of 45 individuals, and 0.151 in the larger population of 61 individuals. The genetic variation was not significantly different between these two populations. Genetic diversity within populations was not low considering the very small size of populations. Analysis of molecular variance (AMOVA) revealed higher variation within populations (65.9%) than genetic differentiation between them (34.1%). B. latissimum revealed higher population differentiation than other outbreeding species. The differentiation of the populations corresponded to low gene flow (Nem = 0.482). The cluster and principal coordination analyses provide strong support for high population differentiation, showing that all individuals of the two populations have built up population-specific clusters. Although gene flow between the two populations of B. latissimum was limited, they have preserved relatively high levels of genetic variation.

Isolation of a Phytase-Producing Bacillus sp.KHU-10 and Its Phytase Production

Choi, Yang Mun,Noh, Dong Ouk,Cho, Sung Ho,Lee, Hyo Ku,Suh, Hyung Joo,Chung, Soo Hyun 한국미생물 · 생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.2

A bacterial strain producing high level of an extracellular phytase was isolated from cooked rice and identified as a strain of Bacillus sp. and designated as Bacillus sp. KHU-10. Optimum culture conditions were investigated for the maximum productivity of phytase by Bacillus sp. KHU-10. 1.0% Maltose and 1.0% peptone with 0.5% beef extract were the best carbon source and nitrogen source, respectively. The addition of CaC1_2 stimulated the enzyme productivity with concentration between 0.01% and 0.2%, in the medium. Although sodium phosphate increased the cell mass, the enzyme activity decreased. Calcium phytate and wheat bran containing phytate did not enhance the enzyme production. Under the optimum medium. the production of the phytase reached the highest level of 0.2 unit/㎖ after 4 days of incubation.

瓔兒性 肝血管內皮腫 2例 報告

朱亮求,金洪,徐修之,禹聖龜 啓明大學校 醫科大學 1987 계명의대학술지 Vol.6 No.2

Hemangioendothelioma of the liver is a rare neoplasm which may be responsible for hepatomegaly or abdominal mass in infancy. We reports two cases of infantile hepatic hemangioendothelioma with exophytic growth from lateral segment of the left lobe. CT showed solitary, large, homogeneous circumscribed area of lower attenuation than that of the liver and spleen in left upper abdomen displacing the stomach medially, and dense peripheral enhancement was shown after bolus injection of contrast medium.

Bacillus sp. LK-1의 Exopolysaccharides(Pol-Ⅱ) 생산 및 특성

김양효,안성구,서현효,김혜자,윤병대 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5

토양에서 분리한 고점도 다당류 생산 균주 LK-1을 동정한 결과 Bacillus sp.로 추정되어 최종적으로 Bacillus sp. LK-1으로 명명하였다. 본 균주가 생산하는 다당류(POL-II)의 발효조에서의 대당 수율은 15.5%(6.5 g/ℓ)였으며 이때 점도는 60,000cp였다. POL-II의 구성당은 glucose : glucuronate : xylose : mannose이며, 그 구성비는 1 : 2 : 1 : 2이다. 한편, POL-II는 대조구 xanthan gum과의 성질 비교에 있어 xanthan gum보다 훨씬 높은 점도를 보이며 1% 요액에서 xanthan gum에 비해 상대적으로 약 10배의 점도를 나타내었다. 또, extreme pH와 50℃이상의 온도 및 NaCl의 첨가에 의해서 점도가 감소하는 현상을 보였다. DSC와 IR spectrum의 결과에서 독특한 특성을 보였으며 수용액상에서 투명하며 음의 하전을 가진 polyelectrolite의 특성을 가지고 있고 film 형성능이 우수한 것으로 판명되었다. The strain which produced highly viscous exopolysaccharides (EPS) in liquid culture was selected from soil. The strain was supposed to Bacillus sp. from the results of morphological, biochemical and physiological tests. The medium composition for EPS productin was trypton 0.75%, sucrose 4%, CaCO_3 0.01%, Winogradsky’s nitrogen free mineral medium 5 ㎖/ℓ and pH 7.0. In 2-ℓ jar fernenter, the viscosity of culture broth after 120-hr cultivation time was very high (60,000 cps) and the amount of EPS was 6.2 g/ℓ. The EPS was composed of glucose, glucuronate, xylose, mannose(1:2:1:2). The viscosity of the EPS which was named POL-II was very higher than that of xanthan gum and substantially decreased under extreme pH condition, high temperature and salt addition.

Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

Hong, Yoochan,Ku, Minhee,Lee, Eugene,Suh, Jin-Suck,Huh, Yong-Min,Yoon, Dae Sung,Yang, Jaemoon SPIE--the International Society for Optical Engine 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.5

In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

Hong, Yoochan,Ku, Minhee,Lee, Eugene,Suh, Jin-Suck,Huh, Yong-Min,Yoon, Dae Sung,Yang, Jaemoon SPIE - International Society for Optical Engineeri 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.5