Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

가토안의 Acid Phosphatase의 활성 및 분포

김태조,손해숙,송미영,이양진 曉星女子大學校 藥學大學 學生會 1990 曉星藥誌 Vol.5 No.-

Lysosomal enzyme activity of choroid, newrosensory retina, iris and ciliarybody, cornea and sclea of the rabbit eye was measured by acid phosphatase(ASPase)as enzyme maker. We reported lysosomal enzyme activity on retinal pigment epitherium in 1998. Distribution of ACPase in the tissues was carried out using Fukuzawa method and Kind-king method. Of the tissues of the rabbit eye the ACPase activity was highest in the choroid(28.6583±12.6920)by Kind-king method. The meurosensory retina(4.5433±2.2091), Iris & ciliary body(2.4138±0.8307), cornea(1.7223±0.6492)and sclea(0.9550±0.0646)also showed the specipic activity of enzyme.

농촌지역 영유아 보육의 문제점

김경희,박분희,양미경 목포대학교 여성문제연구소 2000 여성연구 Vol.8 No.-

Buffer/CoFe/Cu/Co 샌드위치 박막의 자기저항 특성

김희중,김미양,오미영,이장로,송은영,김경민 숙명여자대학교 자연과학연구소 1996 자연과학논문집 Vol.- No.7

DC magnetron sputtering방법으로 Corning glass 기판위에 버퍼층을 Fe와 ??로 바꾸어가면서 보자력이 다른 ??와 Co를 이용하여 buffer/CoFe(35Å)/Cu(tÅ)/Cu(35Å)의 형태로 샌드위치 박막을 제작하고 자기저항비의 버퍼층 두께 및 비자성층 Cu층 두께, 자성층 두께 의존성을 조사하였다. 자기저항비와 포화 자기장(??)은 버퍼층의 두께가 두꺼워짐에 따라 증대하다가 극대치 3%를 보인 후 완만하게 감소하였다. NiFe, Fe버퍼층을 갖는 시료를 비교한 경우, 각각 CoFe층과 Co층 사이의 결합 자기장(??)은 큰 차이가 없었으나 NiFe버퍼층을 갖는 시료가 minor자기저항 곡선의 반가폭 ??는 감소하고 자기저항(MR) slope와 관련된 field senditivity(%/Oe)는 향상되었다. Buffer(tÅ)/CoFe(35Å)/Cu(50Å)/Co(35Å) sandwiches prepared by dc magnetron sputtering on a corning glass substrate using ?? and Co possess different coercivities. Dependence of magnetoresistance on the type and thickness of buffer layer, thickness of Cu and thickness of magnetic layer in buffer/CoFe/Cu/Co sandwiches were investigated. Magnetoresistance ratio and saturation field ?? increased as the thickness of buffer layer becomes thicker, then decreased smoothly after maximum value. Improved field sensitivity was realized by the use of ?? buffer layer.

여성 요실금에서 Kontinence™와 Compact Elite™의 치료효과 비교

양상국,정상원,오미희,노용수,김홍섭,우응제,박승훈,이수열 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-

Biofeedback and pelvic floor electrical stimulation are new modalities that have been advocated for the treatment of female urinary incontinence. The aim of this study was to compare prospectively the therapeutic effect of Kontinence™(HMT Co., Korea) with Compact Elite™(ECL Co., France) in female urinary incontinence. All patients were evaluated with a complete history, physical examination, urinalysis, urine culture and urodynamic study. Eighteen patients were treated with Compact Elite™, and thirteen patients with Kontinence™. Enrolled patients had completed 8 office sessions consisting of biofeedback and intravaginal electrical stimulation according to the program of each machines. Overall treatment response was graded by patients on a scale of complete cure to aggravation. At two weeks after last session, overall improvement rate was 78% in Compact Elite™group and 92% in Kontinence™group(p>0.05). Overall patients' acceptability during treatment course was greater than 80% in both machines(p>0.05). There were no unexpected clinical findings during Kontinence™ treatment. After Kontinence™treatment, there were no laboratory changes including electrocardiography, complete blood count, liver function profile, and chest PA. These results showed that the biofeedback and electrical stimulation with Kontinence™was safe and moderately effective for female urinary incontinence comparable to Compact Elite™. However, long-term results of a larger patients are needed to ensure the therapeutic effect of Kontinence™.

국내산 및 제초제 내성 콩(HS2906)의 일반성분, 무기질 및 지방산 조성

양윤형,이정희,김형진,윤원기,김환묵,김미리 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

Proximate analysis, mineral and fatty acid composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 were evaluated by AOAC method, ICP-AES and gas chromatography. There were several differences in the proximate analysis among three conventional domestic soybean cultivars ; higher crude fat in the cultivar Hwanggumkong, higher crude protein in Pungsankong, and higher carbohydrate and crude ash in Duyukong. The ranges of contents of proximate components of domestic cultivars were similar to the data previously reported. There were no significant differences in proximate analysis between conventional soybean WS82 and glyphosate-tolerant HS2906 ; 23.55~23.90% of crudefat, 34.22~35.55% of crude protein, 6.25~6.45% of crude ash, and 25.35~26.47% of carbohydrate. The mineral and fatty acid compositions of HS2906 were similar to those of conventional soybeans previously reported.

흉선종의 병리학적 검색 및 bcl-2 단백과 MIC-2 단백표현에 대한 면역조직화학적 연구

양경무,조미연,홍순원,김태승,박찬일,양우익 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.5

효율적인 인터넷 검색을 위한 패션정보사이트 분석

이미진,김양원 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.1

인터넷을 이용한 패션정보 사이트를 분석하기 위하여 국내 검색 도구인 야후, 네이버, 알타포탈, 심마니, 한미르, 라이코스, 와카노, 까치네를 이용하였다. Fashion이라는 검색어를 이용하여 검색결과로 나온 각 사이트를 교육정보, 상품정보, 회사정보로 분류하여 방문자수가 10,000회를 넘은 사이트를 분석한 결과는 다음과 같다. 1. 방문자수는 교육정보에 관한 사이트가 가장 많았고, 회사정보에 관한 사이트가 가장 적었다. 2. 교육정보에 관한 사이트는 패션트랜드, 패션쇼, 코디에 관한 내용이 많았다. 3. 상품정보에서는 상품소개가 가장 많았고 다음으로는 쇼핑정보가 많았다. 4. 회사정보를 제공하는 사이트는 다른 정보를 제공하는 사이트보다 방문자수가 적었으며, 주로 회사소개, 상품소개에 관한 내용이 많았다. To analyse the contents of the fashion internet sites, domestic seach engines such as Yahoo, Naver, Altavista, Simmani, Hanmir, Lycos, Wakano, Kachi were used. Searching word was fashion, each site was classified by sites for education, products, and brand information . Also they was analysed the fashion sites that were connected over 10,000times. The results were as followings. 1. The numbers of visitors to fashion internet sites were the most in fashion sites for education and the fewest in the fashion brand sites. 2. The contents of fashion education sites were a lot of fashion trend, fashion show, fashion coordinate. 3. The most contents in fashion products sites were the introduction of fashion products, the next was information of shopping. 4. The contents in fashion brand sites was generally a lot of introduction to brand and products.

Ribbon형 Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35) 합금의 자기특성

장현숙,김미양,이장로,이용호 숙명여자대학교 자연과학연구소 1994 자연과학논문집 Vol.- No.5

급냉법으로 리본형 결정질 Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35)합금을 제조하여 Cr의 조성에 따른 자기적 특성을 조사하였다. 포화자화값은 벌크값보다 컸지만 박막의 경우보다는 작았다. Cr의 함량이 증가함에 따라 포화자화값은 감소하였다. 27 at.% Cr의 경우를 예외로 하면 Cr 함량 증가에 따라 결정립의 크기는 감소하였고, 항자력은 증가하였다. 리본형 Co1-x Crx 는 포화자화값의 온도 의존성이 비정질 시료인 경우와 유사한 경향을 나타내었으며 Cr의 함량이 증가할수록 Curie온도(Tr)는 감소하였다. Cr 함량의 증가에 따라 exchange interaction의 평균자승거리<r²>와 spin wave stiffness 상수D는 감소하는 경향을 나타내었다. 천이금속(Co)의 원자당평균 자기모멘트는 Cr의 증가에 따라 선형적으로 감소하였다. The magnetic cnaracteristics of crystalline Co1-x Crx (x=0.27, 0.29, 0.30, 0.32, 0.35) ribbons prepared by the rapid solidification were investigated. The saturation magnetization for ribbon was greater than that for bulk alloys containg the same amount of Cr. but smaller for the films. The grain size of ribbon became smaller and coercive force increased with increasing Cr contents except case of 27 at.% Cr. The saturation magnetization decreased with increasing Cr contensts. The temperature dependence of saturation magnetization for Co1-x Crx ribbon showed the similar behavior as the amorphous alloy and the Curie temperatures were decreased with the increase of Cr contents. The spin wave stiffness constant and the mean square range of exchange interaction, also were decreased. The average magnetic moment per transition metal atom was linearly decreased with increasing the contents of Cr.

Ni53-Fe47자성박막의 신형전류자기 기전력효과

정한,손희영,김미양,장현숙,이장로,이용호 숙명여자대학교 자연과학연구소 1994 자연과학논문집 Vol.- No.5

진공증착방법으로 제작한 Ni53-Fe47 박막에 관한 신형전류자기 기전력효과의, 전류와 자장 방향이 만드는 각θ의존성이, 자기저항효과의 cos 2θ, Hall 1효과의 경우 sin θ임에 대하여 sin 2θ로 관측되고, 이 효과의 자장에 의존하는 전압변화율이 자기저항효과와 비교할 때 차수가 다르게 큰 것이 조사된다. 이 효과가 2 Carrier형 모델로 이해 할 수 있음을 이론적으로 확인된다. A new Galvanomagnetic electromotive force effect of Ni53-Fe47 thin films is studied. The dependence of this effect on θ, angle between the current and the magnetic field, is found to be the form of sin 2θ, in contrast with that of the magnetoresistance effect cos2θand that of the Hall effect sinθ. Property of this effect is that 1the rate of the voltage variation depending on the magnetic field is extremely large as compared with the magnetiresistance effect. It is theoretically confirmed that this effect is well understood on the basis of the two carrier types model.