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Lee Kyung Jae,Ko Jae Sung,Park Kie Young,Kang Ki Soo,Lee Kunsong,Hong Jeana,Kim Soon Chul,Lee Yoon,Kang Ben,Kim Yu Bin,Kim Hyun Jin,Eun Byung Wook,Cho Hye-Kyung,Kim Yae-Jean,Kim Mi Jin,Lee Jin,Lee Tae 대한의학회 2023 Journal of Korean medical science Vol.38 No.47
Background: Several cases of pediatric acute hepatitis of unknown etiology related to adenoviral infections have been reported in Europe since January 2022. The aim of this study was to compare the incidence, severity, possible etiology, and prognosis of the disease with those in the past in Korea. Methods: The surveillance group collected data between May and November 2022 using a surveillance system. Acute hepatitis of unknown etiology was defined in patients aged < 16 years with a serum transaminase level > 500 IU/L, not due to hepatitis A-E or other underlying causes. For comparison, data from 18 university hospitals were retrospectively collected as a control group between January 2021 and April 2022. Results: We enrolled 270 patients (mean age, 5 years). The most common symptom was fever. However, the incidence was similar between 2021 and 2022. Liver function test results, number of patients with acute liver failure (ALF), liver transplantation (LT), death, and adenovirus detection rates did not differ between the two groups. None of the adenoviruspositive patients in either group experienced ALF, LT, or death. In the surveillance group, adenovirus-associated virus-2 was detected in four patients, one of whom underwent LT. Patients with an unknown etiology showed significantly higher bilirubin levels, a lower platelet count, and a higher LT rate than patients with a possible etiology. Conclusion: The incidence of pediatric acute hepatitis of unknown etiology and adenovirus detection rate have not increased in Korea.
Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis
Yoon, Yae Jin,Han, Young-Min,Choi, Jiyeon,Lee, Yu-Jin,Yun, Jieun,Lee, Su-Kyung,Lee, Chang Woo,Kang, Jong Soon,Chi, Seung-Wook,Moon, Jeong Hee,Lee, Sangku,Han, Dong Cho,Kwon, Byoung-Mog Elsevier 2019 Biochemical pharmacology Vol.163 No.-
<P><B>Abstract</B></P> <P>Metastasis is the leading cause of cancer mortality and cancer cell migration is an essential stage of metastasis. We identified benproperine (Benp, a clinically used antitussive drug) as an inhibitor of cancer cell migration and an anti-metastatic agent. Benp selectively inhibited cancer cell migration and invasion, which also suppressed metastasis of cancer cells in animal models. Actin-related protein 2/3 complex subunit 2 (ARPC2) was identified as a molecular target of Benp by affinity column chromatography with Benp-tagged Sepharose beads. Benp bound directly to ARPC2 in cells, which was validated by pull-down assay using Benp-biotin and label-free biochemical methods such as the drug affinity responsive target stability (DARTS) and cellular thermal shift assay (CETSA). Benp inhibited Arp2/3 function, showing disruption of lamellipodial structure and inhibition of actin polymerization. Unlike Arp2/3 inhibitors, Benp selectively inhibited the migration of cancer cells but not normal cells. ARPC2-knockdown cancer cells showed defective cell migration and suppressed metastasis in an animal model. Therefore, ARPC2 is a potential target for anti-metastatic therapy, and Benp has the clinical potential to block metastasis. Furthermore, Benp is a useful agent for studying the functions of the Arp2/3 complex in cancer cell migration and metastasis.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Improvement of Rice Fertility of Breeding Lines in Cold Water Treatment Screening
Jin Chul Shin,Sang Bok Lee,Jong Min Jeong,Jeong Heui Lee,Jeong Il Lee,O Young Jeong,Yong Hwan Choi,Chang Ihn Yang,Im Soo Choi,Eung Gi Jeong,Woon Ho Yang,Dae Ha Seo,Jong Doo Yae 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-
Jin, Yena,Yoon, Yae Jin,Jeon, Yoon Jung,Choi, Jiyeon,Lee, Yu-Jin,Lee, Joonku,Choi, Sangho,Nash, Oyekanmi,Han, Dong Cho,Kwon, Byoung-Mog Pergamon Press 2017 Biochemical pharmacology Vol.142 No.-
<P><B>Abstract</B></P> <P>The roles and significance of signal transducer and activator of transcription 3 (STAT3) in human cancers have been extensively studied and STAT3 is a promising therapeutic target for cancer drug discovery. During the screening of natural products to identify STAT3 inhibitors, we identified geranylnaringenin (CG902), which decreased luciferase activity in a dose-dependent manner. CG902 specifically inhibited STAT3 phosphorylation at Tyr-705 in DU145 prostate cancer cells and decreased the expression levels of STAT3 target genes, such as cyclin D1, cyclin A, and survivin. Notably, the knockdown of the SHP-2 gene by small interfering RNA suppressed the ability of CG902 to inhibit STAT3 activation and CG902 activated the phosphatase activity of SHP-2 through direct interaction with SHP-2 and induced the phosphorylation of SHP-2. The interactions between CG902 and SHP-2 were confirmed by pull-down assay using biotinylated CG902. The interactions were also further validated by the drug affinity responsive target stability (DARTS) and cellular thermal shift assay (CETSA). The inhibitory effect of CG902 on cell growth was confirmed using the DU145 mouse xenograft model. We propose that CG902 inhibits STAT3 activity through a mechanism that involves the interactions between CG902 and SHP-2, and the phosphorylation of SHP-2, which leads to SHP-2 activation in DU145 cells. CG902 is the first compound to regulate STAT3 activity via the modulation of SHP-2 activity, and our results suggest that CG902 is a novel inhibitor of the STAT3 pathway and an activator of SHP-2, and may be a useful lead molecule for the development of a therapeutic STAT3 inhibitor.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
The effect of silver ion-releasing elastomers on mutans streptococci in dental plaque
Yae-Jin Kim,이동렬,Jin-Yong Lee,Yong-Kyu Lim 대한치과교정학회 2012 대한치과교정학회지 Vol.42 No.2
Objective: The purpose of this study was to investigate the antimicrobial effect of silverized elastomers on mutans streptococci in dental plaque. Methods: Forty patients undergoing orthodontic treatment were randomly placed into 1 of 2 groups. We examined the maxillary right and left central incisors and premolars, and the mandibular right and left canines of all participants. We ligated the right maxillary and left mandibular teeth of the participants in group 1 with silverized elastomers and ligated their contralateral teeth with conventional elastomers. We ligated the left maxillary teeth and right mandibular teeth of group 2 participants with silverized elastomers. Each participant visited the clinic 4 times at 3-week intervals. We applied the elastomers to the teeth on one side of each patient’s mouth during their first visit. During the second visit, the elastomers were removed for microbiological analysis and replaced with steel ligatures. During the third visit, we used silverized elastomers to ligate the teeth contralateral to those treated on the first visit. The elastomers were removed during the fourth visit, and microbiological analyses were performed. We compared the quantity of bacteria on silverized and conventional elastomers at the 0.05 level of significance. Results: The percentage of mutans streptococci was not significantly different in cultures of dental plaque from the silverized and the conventional elastomers (p > 0.05). Conclusions: There was no significant difference between the antimicrobial effect of the silverized elastomers and that of the conventional elastomers.
( Jin Sung Lee ),( Gwang Ha Kim ),( Geun Am Song ),( Jeong Heo ),( Dong Uk Kim ),( Hyun Young Woo ),( Bong Eun Lee ),( Yae Su Jang ),( Byoung Gil Ha ),( Jong Hun Seo ),( Tae Wook Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Some gastric subepithelial tumors (SETs) have malignant potential. But, pathologic diagnosis is challenging. Recently, there are increasing reports about endoscopic resection applied to resect gastrointestinal SETs. We aimed to determine the effi cacy and safety of endoscopic resection for the removal of gastric SETs. Methods: From January 2006 to December 2013, 51 patients were included in this study. They underwent routine upper gastrointestinal endoscopy and endoscopic ultrasonography (EUS). endoscopic submucosal dissection (ESD) and endoscopic mucosal resection (EMR) was used to remove gastric SETs. The results of complete resection rates and complications were analyzed. Results: The ratio of male to female was 18:33 (35.3%:64.7%), and the mean age was 55.6 ± 11.1 years. The median tumor size measured by EUS was 14.2 mm (range 4-80 mm), and the median procedure time was 25 minutes (range 6-140 minutes). En bloc resection was achieved in 44 cases (86.3%) and histologic complete resection (CR) was done in 42 (82.4%). Complete resection was considerably low when tumor originate from proper muscle layer (4/8, 50.0%) than submucosal layer (38/43, 88.4%, p=0.019). Histologic diagnosis was obtained in all cases, including 16 carcinoid tumors, 12 infl ammatory fi brinoid polyps, 6 gastrointestinal stromal tumors and 17 others. Signifi cant bleeding during procedure was occurred in three cases and delayed bleeding was occurred in one case. Perforation was detected in 2 cases. The mean follow-up period was 22 months and any recurrence was not identifi ed. Conclusions: Endoscopic resection is safe and effective method to resect and acquire a histologic diagnosis of gastric SETs. However, to achieve en bloc excision for completely curative aim, novel endoscopic procedure including endoscopic full-thickness resection or conventional surgical wedge resection would also be considered, when tumor originate from proper muscle layer.
Flower color modification through expression of Aquilegia buergeriana F3′5′H in Petunia hybrida
Lee Young Ah,천경성,Shin Ju Young,Kim Jeong Ho,Song Bina,Kim Se Jin,박필만,An Hye Ryun,Kim Yae Jin,이준대,Lee Su Young 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.4
Aquilegia buergeriana is a native plant in Korea with blue fl owers. Flavonoid 3′,5′ hydroxylase ( F3′5′H ) is a key gene involved in the synthesis of delphinidin pigment responsible for the fl ower's blue color. We isolated the F3′5′H from the petals of A. buergeriana ( AbF3′5′H ) and introduced the AbF3′5′H gene into Petunia hybrida using Agrobacterium-mediated transformation. Forty-fi ve plants were acquired from a kanamycin-supplemented medium. Fifteen of these were identifi ed as transgenic plants using polymerase chain reaction (PCR). Quantitative real-time PCR (qRT-PCR) analysis revealed that the AbF3′5′H was expressed in the petal, corolla tube, and stigma of P. hybrida . AbF3′5′H -transgenic plant (T 0 ) fl ower color was darker than that of non-transgenic plants (NTs). Particularly, the stigma color was dramatically changed, from light yellow green (145C) to purple (N77C or N79D). The segregation ratio of the three transgenic (T 1 ) lines was identifi ed as 3:1 by PCR analysis of AbF3 ′5′H and neomycin phosphotransferase-II. The fl ower color change of the transgenic lines (T 1 ) was similar to that of T 0 . qRT-PCR analysis showed that AbF3′5′H -transgenic T 1 lines had a higher AbF3′5′H expression than NT in all fl oral organs. Moreover, delphinidin was confi rmed to be accumulated in both corolla tube and stigma and was enhanced in the petals of AbF3′5′H -transgenic T 1 lines through UPLC analysis. Our fi ndings indicate the role of AbF3′5′H in fl ower color change. These results also indicate the functionality of AbF3′5′H in bluish fl ower modifi cations