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sRNA EsrE Is Transcriptionally Regulated by the Ferric Uptake Regulator Fur in Escherichia coli
Bingbing Hou,Xichen Yang,Hui Xia,Haizhen Wu,Jiang Ye,Huizhan Zhang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.1
Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.
Tianshu Wang,Gongxuan Zhang,Xichen Yang,Yang Lv 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3
In hierarchical structures determined by traditional routing protocols of wireless sensor networks, clustering is not structured and the networks prone to generate scatters, leading to some nodes die quickly. So this paper presents a cluster head preferred hierarchical clustering routing protocol based on G-Means (GHPHC). It uses G-Means algorithm to detect each clustering structure, so that each cluster is Gaussian distribution and it avoids the generation of scatters. At the same time, the cluster head preferred algorithm proposed in this paper gives the corresponding weight for each node of all clusters, thus select a suitable cluster head node for every cluster. Experimental results show that compared to traditional routing protocols, the death rate of nodes in a wireless sensor network which uses GHPHC protocol is more slow, and has a longer life cycle.
Xi Chen,Jian-yang Xue,Hechao Li,Guangxu Tu,Xin Lu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.10
Experimental and theoretical research on the rotational behavior of Multi-cell shaped concrete-filled steel tubular (MCFST) connection under low-period cyclic load is performed. The experimental results showed that the design parameters had significant effects on the elastic rotational stiffness of the connection, and affected the sequence and position of the plastic hinges appearance at the column and beam ends. Furthermore, the damage mechanism of the plastic hinge lines is obtained, and the simultaneous yielding for the plastic hinges results in the rapid increase of the beam end rotation. In order to handle the problem with irregular geometry boundary and uneven distribution of tightening-ring stresses a differential element of an unified material is proposed considering the effective tightening-ring stresses of special-shaped steel tube on confined concrete, which can be used to analyse the whole mechanical and damage performance of the connection. Combining unified design theory and experimental results, the calculation for the elastic bending rigidity of the connection is obtained. Finally, the moment-rotation relation model of the connection is proposed by regression analysis on experimental results, and the theoretical results are observed to agree well with the experimental results.